• 제목/요약/키워드: sKD

검색결과 234건 처리시간 0.036초

An Immunological Approach to ABA Receptor and its Gene

  • Xie Zhou;Jin, Zhen-hua;Zheng, Zhi-fu;Kai Xia;Zhang, Neng-gang;Wan, Yin-sheng;Sang, Yong-ming;Chen, Kao-shan;Liu, Shi-ming
    • 한국식물학회:학술대회논문집
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    • 한국식물학회 1996년도 식물학심포지움 식물호르몬과 신호전달
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    • pp.68-78
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    • 1996
  • Two types of immunoloigcal probes, anti-ABBP Abs, have been developed. The purified ABBP from ABA-C1-BSA-sepharose 4B column was identified by PAGE and appeared in one band of about 56KD, as well as showed a specific binding ability and a high affinity for ABA (Kd2.0$\times$10-9 mol/L). Unexpectedly, the existence of rRNA with a length of around 300 nucleotides could be found, when the ABBP was digested with proteinase K and identified by eletrophorsis on an agarose gel (1%). As a result, about 120 cDNA clones coding maize 17s RNA and only one cDNA clone coding ABBP (24cDNA) were obtained from 200,000 seperated phage plaques by the anti-ABBP pAbs. 24cDNA had 1075bp and contained an open reading frame coding 254 amino acids. The anti-idiotypic Ab raised against an ABA MAb showed the ability of either mimicking ABA or competing with ABA. The localization of ABBPs in plant cell was investigated.

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Clinical features, diagnosis, and outcomes of multisystem inflammatory syndrome in children associated with coronavirus disease 2019

  • Kwak, Ji Hee;Lee, Soo-Young;Choi, Jong-Woon;Korean Society of Kawasaki Diseasety of Pediatric Endocrinology (KSPE),
    • Clinical and Experimental Pediatrics
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    • 제64권2호
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    • pp.68-75
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    • 2021
  • The novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been spreading worldwide since December 2019. Hundreds of cases of children and adolescents with Kawasaki disease (KD)-like hyperinflammatory illness have been reported in Europe and the United States during the peak of the COVID-19 pandemic with or without shock and cardiac dysfunction. These patients tested positive for the polymerase chain reaction or antibody test for SARS-CoV-2 or had a history of recent exposure to COVID-19. Clinicians managing such patients coined new terms for this new illness, such as COVID-19-associated hyperinflammatory response syndrome, pediatric inflammatory multisystem syndrome temporally associated with COVID-19, or COVID-19-associated multisystem inflammatory syndrome in children (MIS-C). The pathogenesis of MIS-C is unclear; however, it appears similar to that of cytokine storm syndrome. MIS-C shows clinical features similar to KD, but differences between them exist with respect to age, sex, and racial distributions and proportions of patients with shock or cardiac dysfunction. Recommended treatments for MIS-C include intravenous immunoglobulin, corticosteroids, and inotropic or vasopressor support. For refractory patients, monoclonal antibody to interleukin-6 receptor (tocilizumab), interleukin-1 receptor antagonist (anakinra), or monoclonal antibody to tumor necrosis factor (infliximab) may be recommended. Patients with coronary aneurysms require aspirin or anticoagulant therapy. The prognosis of MIS-C seemed favorable without sequelae in most patients despite a reported mortality rate of approximately 1.5%.

인공신장 투석기에서 Ultrafiltration이 Clearance에 미치는 영향 (Effect of Ultrafiltration on the Clearance of Artificial Kidney Dialyzers)

  • 장호남;김진곤;박한철
    • 대한의용생체공학회:의공학회지
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    • 제3권1호
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    • pp.3-8
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    • 1982
  • Solute transfer in artificial kidney dialyzers was analyzed using Kedem-Katcha- Isky's description on membrane transport. Mass transfer coefficient, K was deduced from the diffusive clearance of commercial hemodialyzers. It can to seen that Kd increases with the increase of blood flow rate, which means that there is substantial resistance in the blood phase for solute transport. Total clearance was estimated with the Werynski's formula. The increase in total clearance due to ultrafiltration was most significant for middle molecules like vitamin Bla, however that for smaller molecules such as urea and creatinine was minimal.

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부분방전 신호의 누적검출과 뉴럴-퍼지 알고리즘을 이용한 컴퓨터 진단에 관한 연구 (A study on the computer diagnosis that apply Neural-Fuzzy algorithm accumulation detection of Partial Discharge signal)

  • 황경준;염경태;김용갑;김진수
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2007년도 제38회 하계학술대회
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    • pp.1445-1446
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    • 2007
  • In this paper, we have studied for analysis of the partial discharge(PD) signal in power transmission line. The PD signal has estimated as detected signal accumulation of a PRPDA method by using Labview, and analyzed with neural-fuzzy algorithm. With practical PD logic implementation of theoretical detected system and hardware implementation, the device for Hipotronics Company's 22.9kV or 154kV setup have generated and then have applied with 18kV,20kV with 1:1 time probe. It's also used the LDPE 0.27mmt (scratch error 0.05mmt) to sample for making PD. Our new class of PD detected algorithm have also compared with previous PRPDA or Neural Fuzzy algorithm, which has diagnose more conveniently by adding numerical values.

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Time-dependent Degradation of Polyphenol Oxidase in Perilla Fructescens Leaves

  • Kim, An-Keun;Kim, Yoo-Kyung
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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    • pp.142.3-143
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    • 2003
  • Time-dependent PPO activity was determined at $4^\times$C and $30^\times$C. The result of activity determination, PPO extracted by phosphate buffer containing triton x-1l4(tPPO) was more stable than PPO by phosphate buffer(bPPO). The result of electrophoresis, at first a band was appeared at 48kd. After 1-3days a partial degrade band was appeared in bPPO and three partial degrade bands in tPPO. No activity band was appeared in PPOs at $30^\times$C and bPPO at $4^\times$C after 4 days. (omitted)

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New Challenge of the CPU for a Moving-Actuator type Total Artificial Heart

  • Yi, S.W.;Ahn, J.M.;Park, S.K.;Om, K.S.;Jo, Y.H.;Choi, J.S.;Lee, J.H.;Choi, J.H.;Kim, W.E.;Choi, S.W.;Kim, H.C.;Min, B.G.
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1997년도 추계학술대회
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    • pp.118-120
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    • 1997
  • Intel's 80C196KD has been applied at the moving-actuator type total artificial heart ( MA-TAH ) from about early 1990th at our laboratory and it fulfilled its role successively. But the increased need of higher performance brought the other-type of the CPU, and the new challenge of ASIC to the application to the MA-TAH accelerated its need. So the new concept of the utilization of the DSP based CPU to the motor control was surveyed and analyzed deeply or about ew months and the results were introduced and summarized in this paper.

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Influence of Pressure Toasting on Starch Ruminal Degradative Kinetics and Fermentation Characteristics and Gelatinization of Whole Horse Beans (Vicia faba) in Lactating Dairy Cows

  • Yu, P.;Goelema, J.O.;Tamminga, S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권4호
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    • pp.537-543
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    • 1999
  • Whole horse beans (Vicia faba cv. Alfred) (WHB) were pressure toasted at different temperatures of 100, 118 and $136^{\circ}C$ for 3, 7, 15 and 30 minutes in order to determine an optimal heating conditions to increase bypass starch (BPSt) as glucose source which is usually limiting nutrient in highly producing dairy cows in the Netherlands. Starch (St) Ruminal Degradative Kinetics and Fermentation Characteristics of (SRDC) of WHB were determined using in sacco technique in 4 lactating dairy cows fed 47% hay and 53% concentrate according to Dutch dairy cow requirements. Measured characteristics of St were soluble fraction (S), potentially degradable fraction (D) and rate of degradation (Kd) of insoluble but degradable fraction. Based on measured characteristics, percentage bypass starch (BPSt) was calculated according to the Dutch new feed evaluation system: the DVE/OEB system. Pressure toasting temperatures significantly affected starch gelatinization (p<0.01). Degradability of Starch in the rumen was highly reduced by pressure toasting (p<0.01). S varied from 58.2% in the raw WHB (RWHB as a control) to 19.6% in $136^{\circ}C/15min$. S was reduced rapidly with increasing time and temperature (p<0.01). D varied from 41.8% in RWHB to 80.5% in $136^{\circ}C/15min$. D fraction was enormously increased with increasing time and temperature (p<0.01). Kd varied from 4.9%h in RWHB to 3.4%/h in $136^{\circ}C/15min$. All these effects resulted in increasing %BPSt from 29.0% in RWHB to 53.1% in $136^{\circ}C/15min$. Therefore BPSt increased from 93.5 g/kg in RWHB to 173.5 g/kg in $136^{\circ}C/15min$. The effects of pressure toasting on %BPSt and BPSt seemed to be linear up to the highest values tested. Therefore no optimal pressure toasting conditions could be determined at this stage. But among 10 treatments, The treatment of $136^{\circ}C/15min$was the best with the highest BPSt content. It was concluded that pressure toasting was effective in shifting starch degradation from rumen to small intestine to increase bypass starch.

계면활성제에 의해 유도된 피부염증에 있어서 Rose, Lavender essential oil 의 유효성 연구 (Study of Efficiency with Rose, Lavender essential oil about Skin Inflammation Iinduced by Surfactant)

  • 최정숙;남정해
    • 한국의류산업학회지
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    • 제8권4호
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    • pp.471-475
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    • 2006
  • Rose와 Lavender essential oil의 계면활성제 유발피부염증에 대한 유효성 연구결과는 다음과 같다. 단백질 분석결과 Rose essential oil 과 Lavender essential oil 이 피부에 자극은 있지만, 단백질의 과도한 변성을 한다는 것은 거의 없다. 단 43Kd 범위이하 해당되는 단백질의 밴드 변화에 있어서는 대조군의 Rose essential oil 처리군과 Lavender essential oil 처리군 에서만 나타났다. 표피층 관찰결과에서 손상된 처리군은 과각질이 일어났으며 각질층이 상당히 비후된 상태로 나타났고, 3주동안 Rose essential oil 처리군에서는 표피구조가 정상적인 표피 구조로 회복되어 가고 있는 것을 볼 수 있었으며, 반면에 손상군의 Lavender essential oil 처리군에서는 과각화 되었던 박리층이 떨어져 나감을 볼 수 있었다. 기저층의 변화를 관찰한 결과 대조군에서는 Rose essential oil 처리군과 Lavender essential oil 처리군에서 모두 일정한 세포층을 이루었으며, 손상군에서는 Lavender보다 Rose essential oil 처리군이 정상적인구조의 기저층이 발생됨을 확인할 수 있었다. Mast cell 실험에서 보면 손상된 처리군에서 Mast cell의 숫자의 변화는 Lavender essential oil 처리군이 Mast cell의 수가 많아짐을 알 수 있고 손상군에서 보다 세포의 크기가 커지고 수도 많아짐을 알 수 있었다.

김치유래 Pichia속 효모가 생산하는 $L-galactono-{\gamma}-lactone\;oxidase$의 분리 정제 및 특성 (Purification and Characterization of $L-galactono-{\gamma}-lactone$ Oxidase in Pichia sp. Isolated from Kimchi)

  • 오지영;한영숙
    • 한국식품과학회지
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    • 제35권6호
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    • pp.1135-1142
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    • 2003
  • 본 연구는 김치의 발효 말기에 비타민 C의 증가 현상을 조사하였으며, 이는 김치 발효 후기에 생육하는 효모에 의한 현상으로 미생물인 효모에서 비타민 C 생합성 경로를 밝히기 위해 수행된 것으로 그 결과는 다음과 같았다. 김치의 숙성에 따른 pH와 총 산함량의 변화는 김치가 숙성되면서 pH는 담금 초기 5.29에서 숙성 33일에 pH 3.50으로 감소되었고, 총 산함량은 담금 초기 0.24%에서 숙성 33일에 2.48%로 증가되었으며, 비타민 C의 함량을 측정한 결과 발효 3일째까지 점점 상승하다 감소되었으나, 발효 15일째는 19.58mg%로 초기 함량보다 다소 높게 측정되었다. 김치의 PG 활성은 김치 숙성초기에는 낮았으나, 김치 숙성 10일 이후부터 증가되어 발효 13일에는 15.4unit/mg protein으로 PG활성이 증가하는 경향을 보였다. 김치에서 분리된 효모 중 배양액의 비타민C의 함량이 높게 측정된 효모를 선별하였으며, 동정결과 Pichia onychis, Pichia rabaulenis, Pichia jadinii, Candida humilis로 각각 동정되었다. 분리, 동정된 효모 중 비타민 C생성량이 가장 높았던 Pichia onychis 16-4 균주로부터 비타민 C 합성 조효소액을 추출하였으며, specific activity는 7.26unit/mg protein로 나타났다. 최종 정제 시 효소활성은 4,698unit/mg protein으로 나타나 처음보다 647.10배로 농축되었음을 알 수 있었다. 정제된 효소액의 분자량은 31,000, 39,000, 50,000 KD으로 3개의 subunits를 갖는 것이 확인되었다. 정제된 효소는 $L-galactono-{\gamma}-lactone$를 최적 기질로 사용하였으며 $35^{\circ}C$에서 pH 7.8일때 최적활성을 나타내었다. 본 연구에서 정제된 효소는 산소를 전자수용체로 하는 $L-galactono-{\gamma}-lactone$ oxidase임을 알 수 있었다.

알루미나/지르코니아 테이프 케스팅 슬러리의 분산과 레올로지에 미치는 용매혼합의 영향 (Effects of Solvent Mixtures on Dispersion and Rheology of Alumina/zirconia Tape Casting Slurries)

  • 김지현;양태영;이윤복;윤석영;박흥채
    • 한국재료학회지
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    • 제11권6호
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    • pp.519-526
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    • 2001
  • Al$_2$O$_3$/ZrO$_2$ 비수성 현탁액의 분산과 레올로지에 미치는 methyl isobutyl ketone (MIBK) /ethanol (EtOH) 용매혼합의 영향을 침전밀도, 점도를 측정함으로써 조사하였다. 분산제 'Hypermer' KD-1이 첨가된 다량의 MIBK를 함유하는 용매 ($\geq$60vol%)에서 $Al_2$O$_3$와 ZrO$_2$ 입자의 침전밀도는 증가하였다. 80MIBK/20EtOH(vol%)에서 ball milling한 현탁액이 좁고 unimodal한 입자크기분포를 나타내었다. 모든 현탁액은 의가소성유동을 나타내었으나 shear thinning은 $Al_2$O$_3$/ZrO$_2$의 혼합비, MIBK/EtOH의 혼합비에 따라서 다소 다르게 거동하였다. 순수한 MIBK를 사용한 $Al_2$O$_3$(<300 s$^{-1}$ ) 및 ZrO$_2$(<3000 s$^{-1}$ ) 현탁액은 주어진 전단속도범위내에서 가장 강한 shear thinning을 나타내었다. 동일한 용매 (80MIBK/20EtOH, vol%)를 사용한 경우의 shear thinning은 $Al_2$O$_3$/ZrO$_2$의 혼합비에 거의 의존하지 않았다.

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