• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.61-65
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• 1995

Karyotype analysis was carried out in four lines of Bupleurum falcatum L. cultivated in Korea and SDS-PAGE was applied to determine the seed protein profiles among the lines. Chromosomes were classified into two groups, large and small ones. Two kinds of karyotype, 2n=20 and 2n=26, were identified. Chromosome 1 of 2n=20 were all submedian, while that of 2n=26 were median. Chromosomes 2, 3 and 5 of 2n=20 showed polymorphism in size and arm-ratio. Chromosome 2 was submedian, while others were median in the line of 2n=26. Karyotypcs of cultivars native of Korea were similiar each other, while those introduced from Japan showed different patterns. In SDS PAGE gels, qualitative difference s in high molecular weight proteins, more than 45KD, were detected among the lines. The numbers of specific band were three in lines of 2n=20 and two in 2n=26.

• Korean journal of applied entomology
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• v.27 no.4
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• pp.211-218
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• 1988

Polyhedral proteins and the endogenous alkaline protease associated with larval-derived polyhedra of nuclear viruses isolated from Spodoptera litura, Bombyx mori, and Hyphantria cunea were investigated. Polyhedral proteins prepared under alkaline protease heat-inactivated condition were separated as one band with 31Kd in S. litura a H. cunea NpV and 30Kd in B. mori NPV by the SDS-polyacrylamide gel electroptoresis. Whereas polyhedral proteins without heat-inactivation were degraded into smaller polypeptides with a certain pattern in alkaline solution. The results of double-immunodiffusion and western blot analysis with antisera against polyhedral proteins indicated that those three polyhedral proteins had common antigenic determinants and the degradation of polyhedral proteins by alkaline protease could be confirmed.

• 발명특허
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• v.10 no.8 s.114
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• pp.64-64
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• 1985

금성반도체(대표 : 구자두)는 미국, 일본에 이어 세계 3번째로 첨단반도체제품인 CMOS 64K SRAM을 자체개발하는데 성공했다. 국내 최초로 개발된 금성반도체의 CMOS 64K SRAM은 우리나라의 반도체 기술수준을 선진국 수준으로 성큼 다가서게 했다. CMOS 64K SRAM은 NMOS의 256K DRAM에 비해 작동속도가 2배이상 빠를 뿐만 아니라 재충전이 필요없는 완전한 스태틱(static) RAM으로 대용량$\cdot$고속$\cdot$고신뢰성을 요하는 고성능 컴퓨터, 통신장비등 첨단 산업용 기기의 기억장치에 주로 사용된다.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2001.02a
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• pp.35-37
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• 2001

인간 Lactadherin은 비유 개시와 동시에 mammary gland의 epithelial cell에 의해 생성되는 46 Kd의 glycoprotein이다. 이 단백질은 성장기 어린이의 diarrheal disease의 원인이 되는 rotavirus에 specific하게 결합하여 그 증식을 억제시켜서 바이러스성 설사를 예방한다. 본 연구에서는 한국 여성의 lactadherin gene을 cloning 하여 그 sequence를 분석 비교하고 서양 여성과의 유전자 변이를 조사하였다. 보고된 서양 여성의 lactadherin 유전자와 비교하여 한 여성의 유전자에는 silent mutation을 포함하여 3 곳의 mutation이 있었고, 또 다른 여성의 유전자에서 새로운 3 곳의 mutation이 존재하여 이 유전자의 SNP가 여러 곳에 산재되어 있음이 관찰되었다.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.1
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• pp.35-42
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• 1998

Whole faba beans (WFB) were dry roasted at different temperatures (110, 130, $150^{\circ}C$) for 15, 30, 45 minutes to determine the optimal heating conditions of time and temperature to increase nutritional value. Ruminant degradation characteristics of crude protein (CP) of WFB were determined by the nylon bag incubation technique in dairy cows fed 60% hay and 40% concentrate. Measured characteristics of crude protein (CP) were soluble (washable) fraction (S), undegradable fraction (U), lag time (T0), potentially degradable fraction (D) and the rate of degradation (Kd) of insoluble but degradable fraction. Based on measured characteristics, percentage bypass crude protein (%BCP) and bypass crude protein (BCP in g/kg) were calculated. Degradability of CP was reduced by dry roasting (p < 0.01). S was reduced rapidly with increasing time and temperature, from 49.0% in the raw WFB (RWFB) to 26.3% in $150^{\circ}C/45$ min. D varied from 50.7% in RWFB to 73.7% in $150^{\circ}C/45^{\prime}$. U varied from 0% in $130^{\circ}C/45^{\prime}$, $150^{\circ}/30^{\prime}$ and $150^{\circ}/45^{\prime}$ to 0.66% in $110^{\circ}/45^{\prime}$ (0.24% for the RWFB). Lag time (T0) varied from 1.58 h in $130^{\circ}C/30^{\prime}$ to 2.40 h in $150^{\circ}C/45^{\prime}$ (1.87 h for RWFB). Kd varied from 24.2% in the $110^{\circ}C/30^{\prime}$ to 4.3% in $150^{\circ}C/45^{\prime}$ (21.4% for the RWFB). Kd was significantly reduced with time and temperature. All these effects resulted in increasing % BCP from 8.9% in the $110^{\circ}C/45^{\prime}$, 11.3% in the RWFB to 43.1% in the $150^{\circ}C/45$. Therefore BCP increased from 31.3 and 39.9 to 148.4 g/kg respectively. Both %BCP and BCP at $150^{\circ}C/45$ increased nearly 4 times over the raw faba beans. The effects of dry roasting temperature and time on %BCP and BCP seemed to be linear up to the highest values tested. Therefore no optimal dry roasting conditions of time and temperature could be determined at this stage. It was concluded that dry roasting was effective in shifting crude protein degradation from rumen to intestine to reduce unnecessary nitrogen (N) loss in the rumen. To determine the optimal treatment, the digestibility of each treatment should be measured in the next trial using mobile bags technique.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.165-170
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• 1987

This study was undertaken to elucidate the behavior of herbicide bensulfuron-methyl[methyl-2-[[[[[(4,6-dimethoxy pyrimidine-2yl)amino]carbonyl]amino]sulfonyl]methyl]benzoate]in soils. Adsorption of the herbicide in soils was mainly correlated with content of organic matter and clay, and canon exchange capacity. Adsorption distribution coefficient(Kd value) in clay loam soil was greater than those in loam and sandy loam soils. The Kd value decreased in the order of zeolite, bentonite, halloysite and laziolite clay minerals. Bensulfuron-methyl moved to 3cm deep in clay loam soil and 4cm deep in sandy loam and herbicide treated layer was 0 to 2cm profile in the two soils. The decomposition rate of bensulfuron methyl varied with the soil properties. The rate was slower in sterilized soil than in nonsterilized. Addition of organic matters to the soils accelerated the decomposotion. The degradation was more rapid in 30$^{\circ}C$ soil temperature than in 20$^{\circ}C$.

• Pediatric Infection and Vaccine
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• v.24 no.2
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• pp.102-107
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• 2017

Purpose: Coronary arterial lesions (CALs) were reported to have developed in children with systemic inflammatory diseases, as well as those with Kawasaki disease (KD). The purpose of this study was to confirm that the CAL development in children with KD occurs in a mouse model of sepsis presenting typical systemic inflammatory response syndrome (SIRS). Methods: To induce the sepsis mouse model with SIRS, 6-week-old C57BL/6 mice were intraperitoneally injected with endotoxin. We compared histological findings of the major organs between the control and the sepsis groups and examined CAL in the heart of the septic mice. Results: Infiltrating inflammatory cells were relatively increased in the heart, liver, and kidneys of the sepsis group, compared with those of the control group. We confirmed lymphocytic infiltration in the myocardium (myocarditis) and the pericardial soft tissue of the heart. Furthermore, coronary artery of the septic mouse was identified, but CAL was not observed. Conclusions: In this study, we failed to confirm the existence of CAL in a mouse model of sepsis. However, it is well-known that CALs are seen in many kinds of diseases that cause SIRS. Our findings suggest further investigation into the clinical significance of CAL in various systemic inflammatory diseases, including KD.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1619-1624
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• 2003

The effect of maize hybrid (Suco and Dekalb 765, DK 765), maturity stage (milk, $R_3$ and 1/2 milk line, $R_5$) and animal diet (Diet 1: 70% lucerne hay+30% maize silage; Diet 2: 50% maize silage+20% sunflower meal+30% maize grain) on ruminal stover dry matter (DM) degradability was studied. Additionally, morphological and chemical plant composition was evaluated. Fodder samples ground at 2 mm were incubated in three Holstein steers (400 kg body weight) using the in situ technique. Ruminal degradation kinetics was studied and the effective degradability (ED) was estimated for an assumed kp of 5%/h. The in situ data was analyzed in a complete randomized block design with the animals as blocks. Significant interactions between hybrid${\times}$diet and maturity${\times}$diet on kinetic digestion parameters were detected. In Diet 1, hybrids did not differ in degradable fraction, kd or ED, although a minor difference (p<0.05) in the soluble fraction was found (25.5 and 23.2% for Suco and DK 765, respectively). In Diet 2, the DK 765 had greater degradable fraction (p<0.001) but smaller (p<0.01) kd than Suco, without differences in the soluble fraction or in ED. Anticipating the harvest increased ED of stover from 37.5% in $R_5$ to 44.6% in $R_3$ (average values across hybrids and diets) due to the increase (p<0.001) in the soluble fraction ($R_5$: 22.6%, $R_3$: 28.8%). It is concluded that hybrids had similar stover in situ DM degradability and that soluble fraction represent the main proportion of degradable substrates. Advancing the date of harvesting may not improve the in situ DM degradability of whole maize plant silage since the increase in stover quality is counteracted by the depression in the grain-to-stover ratio. The diet of the animal consuming silage might not improve stover utilization either.

• Korean Journal of Food Science and Technology
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• v.22 no.4
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• pp.386-392
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• 1990

Soybean proteins of 19 varieties recommended In Korea were characterized by solubility classes, SDS-PAGE and amino acid composition. In distribution of the protein fractions by solubility difference, glycinin content was $48.19{\sim}58.86%$ of total protein. Prolamin, constituting about 1.16% of total protein, was the fraction showing the significant differences between varieties. The electrophoretic patterns of whole soybean proteins exhibited no varietal differences except in 6 varieties of Padal, Jangbaek, Jangyeob, Danyeob, Nameheon and S-138 in molecular weight range of $21.5{\sim}31.0%$ kd. Cystein, methionine, tyrosine and threonine were the minor components of soybean protein and percentage of tyrosine to the total proteins showed significant varietal differences.

• Korean Journal of Clinical Pharmacy
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• v.27 no.4
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• pp.221-227
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• 2017

Background: As personalized healthcare industry has attracted much attention, big data analysis of healthcare data is essential. Lots of healthcare data such as product labeling, biomedical literature and social media data are unstructured, extracting meaningful information from the unstructured text data are becoming important. In particular, text mining for adverse drug reactions (ADRs) reports is able to provide signal information to predict and detect adverse drug reactions. There has been no study on text analysis of expert opinion on Korea Adverse Event Reporting System (KAERS) databases in Korea. Methods: Expert opinion text of KAERS database provided by Korea Institute of Drug Safety & Risk Management (KIDS-KD) are analyzed. To understand the whole text, word frequency analysis are performed, and to look for important keywords from the text TF-IDF weight analysis are performed. Also, related keywords with the important keywords are presented by calculating correlation coefficient. Results: Among total 90,522 reports, 120 insulin ADR report and 858 tramadol ADR report were analyzed. The ADRs such as dizziness, headache, vomiting, dyspepsia, and shock were ranked in order in the insulin data, while the ADR symptoms such as vomiting, 어지러움, dizziness, dyspepsia and constipation were ranked in order in the tramadol data as the most frequently used keywords. Conclusion: Using text mining of the expert opinion in KIDS-KD, frequently mentioned ADRs and medications are easily recovered. Text mining in ADRs research is able to play an important role in detecting signal information and prediction of ADRs.