• Title/Summary/Keyword: s-LDH

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Using Effective Temperatures to Determine Safety Cultivation Season in Direct Seeding Rice on Dry Paddy (작물생육 유효기온 출현시기를 이용한 건답직파 벼의 지역별 안전작기 설정)

  • 최돈향;윤경민;윤성호;박무언
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.666-672
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    • 1997
  • Twenty years' daily mean air temperature data was used to calculate the critical early seeding date(CESD), the optimum heading date(OHD), the critical late heading date for stable ripening(CHDR) and the critical late ripening date(CLRD) for rice seeded on dry paddy in different agroclimatic zones in Korea. The CESD was defined as the first day with mean air temperature of 13$^{\circ}C$, and the OHD as the first day of the 40 consecutive days with mean air temperature of 22$^{\circ}C$ or above after heading. The CHDR was defined as the date after which the cumulative daily mean air temperature would be at least 76$0^{\circ}C$. Lastly, the CLRD was defined as the last day when daily mean air temperature remains above 15$^{\circ}C$. This information was used for the estimation of periods from the earliest date of seeding to optimum heading date, the latest possible date of heading and the latest possible date of ripening in respective regions. For instance, in Suwon, those respective periods mentioned were found to be 104days, 124days, and 165days.

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Comparison of Egg Productivity, Egg Quality, Blood Parameters and Pre-Laying Behavioral Characteristics of Laying Hens and Poor Laying Hens (산란계와 과산계의 난생산성, 계란품질, 혈액 특성 및 산란 전 행동 특성의 비교)

  • Woo-Do, Lee;Hyunsoo, Kim;Jiseon, Son;Eui-Chul, Hong;Hee-Jin, Kim;Hwan-Ku, Kang
    • Korean Journal of Poultry Science
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    • v.49 no.4
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    • pp.189-197
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    • 2022
  • This study was conducted to compare the egg productivity, egg quality, and blood characteristics of laying hens with different laying rates, and the frequency and cumulative duration of the sitting behavior observed before laying was investigated. Twelve 45-week-old Hy-Line Brown laying hens were randomly assigned to two treatment groups with three replicates. Treatment groups were classified as layers laying over 80%(high egg performance layers; HEP) and layers laying below 50%(poor egg performance layers; PEP). The experiment lasted 4 weeks. HEP showed higher hen-house egg production ratio and egg mass and lower feed conversion ratio(FCR) (P<0.05) compared with PEP, although egg weight was higher in PEP (P<0.05). In terms of egg quality, PEP showed differences in eggshell quality (eggshell color, eggshell thickness, and eggshell weight) (P<0.05). Additionally, HEP showed high triglycerides(TG), and PEP showed high alanine transaminase(ALT) level (P<0.05) in serum collected in the morning. In the afternoon, the HEP showed higher lactate dehydrogenase(LDH) levels (P<0.05). No differences in the Ca: P ratio were observed between layers with different laying rates. One hour before egg laying, HEP exhibited sitting behavior 4 times on average, each lasting 25 minutes. In conclusion, egg production and quality differ between HEP and PEP, and HEP showed frequent sitting behavior before egg laying. However, additional research is necessary to explore approaches other than specific behavioral observation to distinguish poor layers in the flock for application in farms.

Changes in Sink capacity and Source Activity of Rice Cultivars in Response to Shift of Heading date (벼 품종들의 출수기에 따른 동화산물 생산능력 및 수용기관 크기 변화)

  • Lee, Sok-Young;Kwon, Yong-Woong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.2
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    • pp.260-267
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    • 1995
  • In temperate zone planting rice at different date subjects the Crop to different climatic condition. The present study aimed at comparison of the change in source-sink relationship of the Japonica(J) and that of IndicaxJaponica(I$\times$J) type rice cultivars caused by shift of heading date. Two J- and two I$\times$J-type cultivars were made to head on August 16, August 26, and September 5. Sink capacity was changed by shift of heading date in different mode between the types of cultivars. In both types major determinant of sink capacity was number of effective tillers, and the number of spikelets per panicle was the minor. In J-type earlier planting/heading was beneficial to increased panicle numbers and this was due mainly to a larger diurnal difference in temperature. I$\times$J-type cultivars favored a higher daily mean temperature to increase the sink capacity. The ability of source at heading, in terms of leaf area per panicle, chlorophyll content per spiklet, photosynthetic ability of leaves per unit area at 25$\^{\circ}C$, carbohydrate and N contents of leaves, was not so different among different heading dates in both types. However, the source activity was governed principally by temperature during grain filling. The J-type cultivars headed on Sept. 5 and I$\times$J-type cultivars headed later than August 16 could not have had sufficient source activity in grain filling due to lower temperature.

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Popping Mechanism and Shape Moulding Factor of Popcorn (튀김옥수수의 파열방향 및 튀김형태 결정요인)

  • Kim, Sun-Lim;Park, Seung-Ue;Kim, E-Hun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.98-102
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    • 1995
  • Popped popcorn generally have a regular popping direction and typical shape. But the reason and mechanism are not clear yet. This experiment was carried out to investigate the shape moulding factor of popped popcorn. Pericarp thickness of tip-cap section of kernels is slightly thicker than that of top section and this fact provides the important information to the reason. Popping starts when the moisture pressure of heated popcorn is increased and reaches at the critical pressure. Therefore, in the same moisture pressure conditions, top sections are bursted first because their pericarp section is thinner than that of tip-cap section. At the very moment tip-cap sections pull down the top sections of peri carp as bi-metal does. So kernels which removed tip-cap section showed the irregular popping shape because they lost the tip-cap pericarp function. How-ever, kernels which removed embryo showed the typical popping shape but their popping volume was small due to emition and shortage of critical moisture pressure. But kernels which removed the whole pericarp and top pericarp were not popped at all because moisture was entirely emitting out of kernels. These results suggest that the shape moulding factor of popped popcorn is the pericarp thickness differences between the top and tip-cap section of kernels.

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Studies on the Varietal Response of Soybeans to Nitrogen Application Level under Different Soil Acidity II. Effect of pH and Nitrogen Application on the Growth and Yield of Soybean Cultivars (대두의 토양산도에 따른 질소반응 연구 II. 토양 및 양액의 산도와 질소시용량에 따른 대두의 생육 및 수량반응)

  • Lee, Hong-Suk;Kwon, Oh-Ha;Ahn, Yong-Tae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.2
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    • pp.103-111
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    • 1988
  • This study was carried out with two cultivars under two levels of pH and four levels of nitrogen fertilization in a field and nutri-culture experiments to obtain the information about the effects of pH and nitrogen fertilization on the growth and yield of soybean. Acidic condition suppressed the growth of soybean plants, and thus yield and yield components of soybean decreased under acidic condition. But they increased with increased nitrogen fertilization. Especially, these respones were more remarkable under acidic condition and in the variety Jangbaegkong. Grain yield of soybean were highly correlated with the content of allantoin and total nitrogen of soybean plants in the variety Jangbaegkong, but this was not in the variety Danyeobkong. The content of protein and fat of soybean seeds decreased under acidic condition, and more nitrogen fertilization increased the protein content, but decreased the fat content.

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Effects of Hot Water Extracts from Lentinus edodes on Hepatic Functional Enzyme Activities in the Rat Fed Butter Yellow(p-Dimethylaminoazobenzene) (표고버섯 열수 추출물이 발암원을 급여한 흰쥐의 간 기능 관련 효소활성에 미치는 영향)

  • 최미연;정수자;임상선
    • The Korean Journal of Food And Nutrition
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    • v.11 no.1
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    • pp.114-122
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    • 1998
  • This study was designed to observe the effect of hot water soluble polysaccharides extract(PS) from Lentinus edodes on the enzyme activities related with hepatic function and peroxidation in the rats fed better yellow. The four groups of male SD rats were fed with the diets contained 15% casein(basal diet; NO group), added butter yellow(BO group) or /and PS(NP, BP group) for 6 weeks. The activities of ${\gamma}$-GTP and GPT in BP were significantly lower compared with BO. The activities of glutathione peroxidase, catalase and lactate dehydrogenase were not significantly different between NP and NO, while those activities were significantly lower value in BP than BO. The activities of glutathione S-transferase of the microsomal and cytosol fractions were significantly lower in BP than in BO. The contents of glutathione and malondialdehyde in the liver were considerably low value in BP. In a view of these results the PS of Lentinus edodes prevents the lipid peroxidation and diminishes the liver toxicity caused with better yellow. The superoxide dismutase activity in cytosolic fraction of liver was not found any effect in all groups. But hepatic function enzyme activities such as catalase and glutathione peroxidase, LDH activities were remarkably decreased in the groups 2(basal diet + PS) and the ${\gamma}$-GTP, GOT and GPT activities, too. In liver, the contents of glutathione decreased by PS supplementation but HDL-cholesterol and total cholesterol ratio in plasma decreased at the groups 3, 4. The ${\gamma}$-GTP, GOT and GPT in plasma were remarkably higher in the rats fed the p-DAB than the control group, too. But above enzyme activities significantly decreased in the groups fed PS.

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동굴생물의 특성과 보전

  • 김병우
    • Proceedings of the Speleological Society Conference
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    • 1995.09a
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    • pp.39-44
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    • 1995
  • $\bigcirc$ 동굴생물은 식물과 동물, 미생물로 크게 구분되지만 태양광선이 완전히 차단된 동굴내 환경에서는 동굴 동물들이 주종을 이루고 있다. $\bigcirc$ 동굴내의 동물에 관한 연구는 1966년 고씨동굴, 용담굴을 시발로 고수굴, 천동굴, 노동굴, 백룡글, 대이굴, 환선굴 등 강원도내에 분포하는 동굴 중 약 30% 정도는 동물을 포함한 생물상이 조사되었다. $\bigcirc$ 강원도의 동굴동물로서 확인된 것은 9강 30목 141종이며 강별로는 곤충강이 9목 50종 주형강이 4목 49종 갑각강이 6목 18종의 순으로 우세하고 목별로는 거미목이 15과 39종, 톡토기목이 7과 20종, 딱정벌레목이 8과 15종으로 우세한 편이다(남, 1987). $\bigcirc$ 동굴생물에 관한 연구는 육상생태계와 격리된 환경에서 생리, 생태적 적응을 통한 종의 분화나 유전, 진화문제를 추구하며 지하생태계와 육상생태계의 생물적인 유연관계 규명에 중요한 분야로 인정되며 아울러 독특한 특성을 지닌 동굴생물의 생존을 위한 방안과 노력이 일부동굴의 개방 및 개발에 앞서 더욱 구체적으로 모색되고 증대되어야 한다. $\bigcirc$ 동굴환경의 주요 특성은 첫째 햇빛에 차단되어 암흑상태이며, 둘째 내부습도가 높고 기온이나 수온의 년중변화가 심하지 않고, 셋째 먹이연쇄에 필수적인 영양공급원이 제한되어 있다. 따라서 광합성작용으로 성장하는 녹색식물은 태양광선이 유입되는 곳이나 인공조명시설 지역을 제외한 곳에서는 서식이 불가능하고 대형동물이나 초식동물은 생존이 어렵다. $\bigcirc$ 동굴내에는 환경에 적응하는 종들만이 서식할 수 있고 이들을 생태적 특성에 따라 세가지로 구분 할 수 있다. 환경적응 요인으로는 광도, 습도, 온도 영양공급원과 섭식장소, 수중생물의 경우는 특히 수온, 수량 영양원등이다.(중략). 본 연구의 접근방법으로는 ASRS의 개념적인 Reference Model을 수립하고 이 Reference Model에 대한 Formal Model로 DEVS(Discrete Event System Specification)을 이용하여 시스템을 Modeling하였다. 이의 Computer Simulation을 위하여 DEVS형식론 환경에서의 Simulation Language인 DEVSim ++ⓒ를 이용하여 시스템을 구현하였다.. 실형 결과로는 먼저 선형 상미분방정식의 예로 mass-damper-spring system, 비선형 상미분방정식의 예로는 van der Pol 방정식, 연립 상미분방정식의 예로는 mixing tank problem 등을 보였으며, 그의 공학에서 일어나는 여러 가지 문제들도 다루었다.화물에 대한 방어력이 증가되어 나타난 결과로 여겨지며, 또한 혈청중의 ALT, ALP 및 LDH활성을 유의성있게 감소시키므로서 감잎 phenolic compounds가 에탄올에 의한 간세포 손상에 대한 해독 및 보호작용이 있는 것으로 사료된다.반적으로 홍삼 제조시 내공의 발생은 제조공정에서 나타나는 경우가 많으며, 내백의 경우는 홍삼으로 가공되면서 발생하는 경우가 있고, 인삼이 성장될 때 부분적인 영양상태의 불충분이나 기후 등에 따른 영향을 받을 수 있기 때문에 앞으로 이에 대한 많은 연구가 이루어져야할 것으로 판단된다.태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([$[W1]_{XO-}$ $[W1]_{XO}$ ]$_{XO}$)로 그리고 [-wh] 의미의 겹의문사는 중복된 발은을 지닌 한 단어로 ([W]$_{XO}$

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Protection effect of New-Yeolda-Hanso tang against $\beta$-Amyloid Induced Cytotoxicity in NGF-differentiated PC12 Cells ($\beta$-Amyloid로 유도된 신경독성에 대한 열다한소탕(熱多寒少湯) 가감방(加感方)의 항(抗)치매효과)

  • Bae, Na-Young;Yang, Hyun-Ok;Ahn, Taek-Won
    • Journal of Sasang Constitutional Medicine
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    • v.21 no.3
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    • pp.138-153
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    • 2009
  • 1. Objectives: Yeolda-Hanso tang (YH) has long been used as traditional herbal formula in Korea as various diseases. Now we modified Yeolda-Hanso tang (YH) for neurodegenerative diseases treatment and named New-Yeolda-Hanso tang (NYH). We investigated neuroprotective effects of NYH on NGF-differentiated PC12 cells cytotoxicity induced by $\beta$-Amyloid peptide (A$\beta$25-35) and evaluated the ability of NYH to prevent and treat for neurodegenerative diseases via autophagy enhancement. 2. Methods and Results: 1) Protective effect of NYH on PC12 cells cytotoxity induced by A$\beta$25-35. PC12 cells survival was measured by MTT and lactate dehydrogenase (LDH) assay. $20{\mu}M$ $\beta$-Amyloid peptide (A$\beta$25-35) induced cytotoxicity on NGF-differentiated PC12 cells. NYH attenuated the cytotoxic effects of A$\beta$25-35 in a dose-dependent manner. 2) Pharmacological induction of Autophagy by NYH in PC12 cells Autophagy induction and activation was measured by immunoblot assay. Marker of autophagy, LC3 II expression and the ratio of LC3-II/I was slightly increased in the protein treated with YH, and significantly augmented in the protein treated with NYH. NYH-induced increase of LC3-II protein level was inhibited by 3MA. 3) Induction of Autophagy by NYH on A$\beta$25-35-induced injury in PC12 cells In MTT assay, $100{\mu}g/ml$ re-treated NYH attenuated $20{\mu}M$ A$\beta$25-35-induced cytotoxicity in PC12 cells. Protection effect of NYH was blocked by autophagy inhibitor 3MA. In immunoblot assay, $1200{\mu}g/ml$ pre-treated NYH activated autophagy in $20{\mu}M$ A$\beta$25-35-induced cytotoxicity in PC12 cells. The observed effect was partially blocked by 3MA. 3. Conclusions: All the results indicated that NYH possesses neuroprotective potential partially mediated by autophagy enhancement and NYH may be considered to be a promising new herbal formula to prevent and treat for neurodegenerative diseases including Alzheimer's disease (AD).

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Effects of Ojeoksangamibang Extract on the Recovery of Liver Function in $CCl_4$-exposed Rats (오적산가미방(五積散加味方) 추출물이 $CCl_4$ 중독 Rat의 간기능 회복에 미치는 영향)

  • Lee, Soo-Yeon;Park, Won-Hyung;Cha, Yun-Yeop;Lee, Eun
    • Journal of Korean Medicine Rehabilitation
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    • v.23 no.3
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    • pp.45-53
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    • 2013
  • Objectives The objective of present study was to investigate the effect of Ojeoksangamibang ($W\check{u}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) extract on recovery of liver function in carbontetrachloride ($CCl_4$)-exposed rat. Methods Male rats weighing $230{\pm}7.21g$ fed experimental diet for 1 week and 28 rats were divided into 4 groups. Each of 7 rats was devided into a control group and experimental groups. We fed a control group of rats a basal diet and administered normal saline (100 mg/kg, 1 time/1 day) for 3 weeks. And we fed each experimental group of rats basal diet and administered an extract of Ojeoksangamibang extracts (100 mg/kg, 200 mg/kg, 300 mg/kg, 1 time/1 day) for 3 weeks. We measured lipid of plasma and liver, concentration of proinflammatory cytokines ($IL-1{\beta}$, IL-6 and IL-10). Statistical analysis was done by one-way analysis of variance (ANOVA) and Duncan's multiple range test with significance level at p<0.05. Results Plasma a-fetoprotein (AFP) and total protein concentration showed a tendency to decrease in Ojeoksangamibang extract-treated groups. However, plasma albumin concentration showed no significant differences in all treatment groups. Activity of plasma Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) in the Ojeoksangamibang extract-treated groups, increased addition amount of Ojeoksangamibang extracts tended to decline. Alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and ${\gamma}$-glutamyl transferase (${\gamma}$-GT) activities showed a tendency to decrease in Ojeoksangamibang extract-treated groups, increased addition amount of Ojeoksangamibang extracts tended to decline. Concentration of plasma triglyceride and total cholesterol showed a lower value than that of control group. The liver $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ concentration were decreased, and IL-10 was increased in Ojeoksangamibang extract groups, compared to control group. Plasma $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ concentration were decreased, and IL-10 was increased in Ojeoksangamibang extract groups, compared to control group. Conclusions This study suggested that Ojeoksangamibang may alleviate liver inflammatory reaction induced by liver toxicity.

The Study on Mucin Release by Airway Goblet Cells in Primary Culture

  • Yang, Ji-Sun;Kim, Ok-Hee;Roh, Yong-Nam;Yi, Sook-Young;Park, Ki-Hwan;Rheu, Hang-Mook
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.04a
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    • pp.89-89
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    • 1995
  • Surface epithelial cells isolated from hamster tracheas and grown on a thick collagen gel become a highly enriched population of mucus-secreting cells. Epithelial cells from tracheas of hamsters were collected using enzymatic procedures and cultured under various conditions. The medium used consisted of a 1:1 mixture of medium 199 and Dulbecco's modified Eagle's (DME) medium which was conditioned before use. Insulin, transferrin, hydrocortisone, epidermal growth factor, and extract from bovine hypothalamus were used as supplement. Due to relatively low basal rates of min secretion from in vitro cultures, cultures are generally radiolabeled using $^3$H-glucosamine as a metabolic precursor. The radiolabeled mucinsreleased are quantitated by precipitation with TCA/PTA. Using this cell culture system, we investigated mucin release of goblet cells by altering the media bathing the apical surface of hamster tracheal surface epithelial(HTSE) cells. Acidic media added sulfuric acid caused sigcificant increases in mucin relesse (155${\pm}$20% at pH 4 and 146${\pm}$16% at, pH 5). Ammonium hydroxide also increased mucin release at pH 9.0(156${\pm}$17%) and pH 10(295${\pm}$9%) respectively. This additional mucin release seems to be associated with cell membrane damage as indicated by release of cellular LDH. SP stimulates secretion of mucin in cultured HTSE cells(154${\pm}$16% at 1${\times}$10$\^$-6/M and 165${\pm}$25% at 1${\times}$10$\^$-5/M. PAF at 5${\times}$10$\^$-6/M and 5${\times}$10$\^$-5/M enhanced by HTSE cells in vitro 168${\pm}$34% and 259${\pm}$30% of mucin secretion, respectively. The increase in mucin release by PAF and SP was not secondary to cell damage or necrosis. SP and PAF may be in mediating mucous secretion induced by inflammation irritantion and infection.

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