• 생명과학회지
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• 제25권3호
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• pp.349-356
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• 2015

최근 차세대염기서열해독법(Next Generation Sequencing, NGS)의 급속한 발전에 힘입어, 다양한 가축 종에 대한 전장유전체 수준의 해독 및 분석 연구수행이 가능하게 되었다. 소의 경우 현재 한우, 칡소, 흑우, 제주흑우 4품종의 재래소가 국제연합식량농업기구 가축다양성 정보시스템에 등록돼 있는 상태이다. 이러한 재래유전자원은 최근 NGS 기술을 이용 전장유전체에 걸친 대용량의 단일염기다형성 정보를 얻는데 성공하였으며, 또한 한국 재래소품종이 유럽기원의 소 품종들과 유전학적으로 차이가 있다는 점이 밝혀졌다. 또한 소 유전체학 분야에서 이 NGS의 응용은 유전체의 구조적 변이 특히 종전 대용량으로 정확한 발굴이 어려웠던 전장유전체에 널리 퍼진 복제수변이의 발굴에 성공적으로 적용되었다. 이러한 일련의 성공에도 불구하고 최근 NGS를 이용한 연구는 내재적인 한계점이 있었는데, 이는 연구 당시 고가의 연구비용 및 분석의 난해함으로 인해 각 대표 소 품종의 단수 또는 소수 개체에 대해서만 적용되었다는 점이 그 대표적 예라 할 수 있을 것이다. 즉, NGS에서 파생된 데이터의 보다 정확한 생물학적 의의를 찾기 위해서는 추가 실험적 검증과 더불어 면밀한 해석이 필요하다는 점을 시사하는 것이다. 최근 차세대염기서열 해독 비용이 지속으로 하락하고 있으며, 이는 단수개체가 아닌 집단수준에서의 NGS 적용이 가능해 짐에 따라 다양한 집단유전체학적 이론이 접목된 연구가 가능해지고 있다. 현재 국내 재래소 품종에 대한 집단수준에서의 연구는 극히 미흡한 상태이나, 이러한 상황은 최근 고밀도 칩, 차세대염기서열 자료와 같은 대용량 유전정보를 생산, 분석 중에 있어 재래가축에 대한 집단수준에서의 연구가 일부 해소될 것으로 기대된다.

• Journal of Preventive Medicine and Public Health
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• 제38권2호
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• pp.147-153
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• 2005

Objectives: Studies on the geographical differences in mortality tend to use a census population, rather than a registration population, as the denominator of mortality rates in South Korea. However, an administratively determined registration population would be the logical denominator, as the geographical areas for death certificates (numerator) have been determined by the administratively registered residence of the deceased, rather than the actual residence at the time of death. The purpose of this study was to examine the differences in the total number of a district population, and the associated district-specific mortality indicators, when two different measures as a population denominator (census and registration) were used. Methods: Population denominators were obtained from census and registration population data, and the numbers of deaths (numerators) were calculated from raw death certificate data. Sex- and 5-year age-specific numbers for the populations and deaths were used to compute sex- and age-standardized mortality rates (by direct standardization methods) and standardized mortality ratios (by indirect standardization methods). Bland-Altman tests were used to compare district populations and district-specific mortality indicators according to the two different population denominators. Results : In 1995, 9 of 232 (3.9%) districts were not included in the 95% confidence interval (CI) of the population differences. A total of 8 (3.4%) among 234 districts had large differences between their census and registration populations in 2000, which exceeded the 95% CI of the population differences. Most districts (13 of 17) exceeding the 95% CI were rural. The results of the sex- and age-standardized mortality rates showed 15 (6.5%) and 16 (6.8%) districts in 1995 and 2000, respectively, were not included in the 95% CI of the differences in their rates. In addition, the differences in the standardized mortality ratios using the two different population denominators were significantly greater among 14 districts in 1995 and 11 districts in 2002 than the 95% CI. Geographical variations in the mortality indicators, using a registration population, were greater than when using a census population. Conclusion: The use of census population denominators may provide biased geographical mortality indicators. The geographical mortality rates when using registration population denominators are logical, but do not necessarily represent the exact mortality rate of a certain district. The removal of districts with large differences between their census and registration populations or associated mortality indicators should be considered to monitor geographical mortality rates in South Korea.

• Journal of Nutrition and Health
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• 제42권4호
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• pp.374-385
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• 2009

This study aims to develop an index of food security in order to assess food security status in Korea and to evaluate reliability and validity of the index. The index of food security was developed based on the US Household Food Security Survey Module (US-HFSS). After the US-HFSS was translated and back-translated, it was evaluated and modified by a focus interview for experts. The developed Korean Household Food Security Survey Module (K-HFSS) was tested by cognitive interviews and a pretest for general community population to assess its application for Korean population. For the reliability and validity of K-HFSS, the survey was conducted with 300 adults aged 40 years or more living in a rural community and 212 infants and toddlers living in an urban community. The reliability was assessed by Cronbach' alpha and the validity was evaluated by content validity, construct validity, and criterion-related validity. The questionnaire items of K-HFSS were partly modified, accounting for Korean social and cultural backgrounds and adapted by general community population. The reliability was relatively high, showing Cronbach' alpha coefficients ranged from 0.80 (for adults) to 0.87 (for infants and toddlers). The content and construct validities were all acceptable. The result of criterion-related validity showed that food security status was significantly related to the household income level. In conclusion, the K-HFSS would be used as a reliable and valid instrument to assess food security status in parts of the Korean population.

• Journal of Ecology and Environment
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• 제33권4호
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• pp.317-323
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• 2010

Nutrient availability is a critical component of agroecosystems, and is relevant to both above- and below- ground interactions. The principal objective of this study was to determine how the cessation of fertilization affects the communities of weeds and soil organisms in a corn/wheat field. Changes in dominant weed species, substrate-induced respiration, and the population density of nematodes and microarthropods were evaluated. Microbial substrate-induced respiration (SIR) and the population density of microarthropods decreased following the cessation of fertilization and were partly correlated with the aboveground weed biomass. The cessation of organic fertilizer application but continuing application of inorganic fertilizer reduced the population density of nematodes. In response to the cessation of fertilization, weed communities were dominated by species with little dependency on fertilization. Amaranthus retroflexus was identified as the most dominant species in the corn field; however, it was replaced by Digitaria ciliaris after the cessation of fertilization. In the wheat field, the cessation of fertilization led to a rapid reduction in the biomass of most weeds, except for Vicia angustifolia, supposedly as the result of symbiotic nitrogen fixation. Additionally, the fact that weed biomass was partially correlated with SIR or the population density of microarthropods may reflect a mutual feedback between soil organisms and weeds. The results indicate that the cessation of fertilization alters communities of weeds and soil organisms through changes in weed biomass and interactions with symbiotic microorganisms.

• 한국농공학회논문집
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• 제60권3호
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• pp.37-50
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• 2018

Local climate characteristics for both urban and rural areas can be attributed to multiple factors. Two factors affecting these characteristics include: 1) greenhouse gases related to global warming, and 2) urban heat island (UHI) effects caused by changes in surface land use and energy balances related to rapid urbanization. Because of the unique hydrological and climatological characteristics of cities compared with rural and forested areas, distinguishing the impacts of global warming urbanization is important. In this study, we analyzed anthropogenic climatic changes caused by rapid urbanization. Weather elements (maximum temperature, minimum temperature, and precipitation) over the last 60 years (1955-2016) are compared in urban areas (Seoul, Incheon, Pohang, Daegu, Jeonju, Ulsan, Gwangju, Busan) and rural/forested areas (Gangneung, Chupungnyeong, Mokpo, and Yeosu). Temperature differences between these areas reveal the effects of urbanization and global warming. The findings of this study can be used to analyze and forecast the impacts of climate change and urbanization in other urban and non-urban areas.

• 교육시설 논문지
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• 제19권3호
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• pp.21-31
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• 2012

According to the decrease in birth rate and reduction of population in rural area, primary schools have brought about many problems for decrease of number of student, and environment of education have became deteriorated. Accordingly, primary schools be required improvement, and it should be altered placement of schools because it change number of student, distance and time of attending school, and formation of school zone are changed by alternating of human and physical environment. Merger and abolition of primary school is inevitable for change of placement standard to primary school and qualitative improvement of environment of education in rural area. This study suggests the effective space planning methods for unified school and appropriate utilization alternatives for closed school following after the merger and abolition of small rural schools. In doing so, it explores the present models of merger and abolition through analysis of all sort of actual condition and the possibilities for various utilizations of the abolished schools. It proposes a classroom-operation type fused the comprehensive class and departmental class system for merged primary schools in rural area. It also suggests the re-use methods of closed school facilities for students and community. Finally, it emphasizes that in order to success the policy of the merger and abolition of small schools, it be attributed to the social-cultural environment based on local residents and community and required provision of a variety of utilization plans reflecting local specifics and guarantee of local residents' participation and selection.

• 농촌계획
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• 제21권2호
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• pp.177-188
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• 2015

This research was establishment of landscape formation plan to Yusang village region Yeongcheon city. Literature research, field research, surveys carried out for this purpose. In addition, by utilizing design code that appears in the common characteristics of the rural scenic views of the region in improving the quality of life of the local population and preserve the indigenous landscape as well as improve the quality of rural landscapes, management, capital town of the region to form Yeongcheon the center was established in the landscape plan form. The natural environment of the capital region for the landscape formation plan, Humanities and Social Environment, Landscape Resources Status, Social Consciousness, landscape map, landscape design, code identification and basic design and planning through the basic steps to derive design guidelines were applied to the landscape formation plan. Formation landscape plan for the regional launch of the regional landscape improvements, region entrance space and yusang reservoirs place, rest zone in the sacred tree and nurse trees around the plan, and repair the town hall. residents living village road maintenance to improve the environment, and develop a detailed plan, such as the lungs maintenance. The plan is likely to be able to contribute to improving the quality of life of the people and landscapes to improve regional, creating a guide for future business landscape is formed to have a significant advantage as a basis.

• 가정과삶의질연구
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• 제27권1호
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• pp.61-71
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• 2009

The purpose of this study was to resolve the issues of inferior housing environment and the population decrease in rural community by improving the environment and attracting urban inhabitants. A simulation on the costs and the local programs was operated from a point of view that Pension Insurance House with Long-term Lease and a plan for the program in connection with local resources should be accompanied to attract urban inhabitants. The study was carried out through mainly documents analysis and specialists' opinions. The simulation results are as follows. Firstly, the pre-existing rural housing development projects have only emphasized the hardware, while underestimated the post-management with operating programs. The software should be underlined when Pension Insurance House is developed. Secondly, as a result of the simulation on construction expenditure and the operating and maintenance cost for 30 years, about 82.3 million Wons are necessary residential expenses for 15 years per unit. Thirdly, in case of MUJU County, it has made the most of its pre-existing institutions. It's medical institutions provide medical care system with health education, facilities related leisure and culture offer recreational programs and the local community center and its program of each town helps new habitants adopt to the rural life. Additionally, the employment project of a local welfare center allow people living in a rural community to continue their careers with their talents and interests through local class programs for a life worth living. Lastly, guide for getting information of rural life, local community gathering and preliminary education should be carried out to reduce expectant tenants' incompatibility and assist them settle down early. The community program expansion is also required at the local government level.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.148-148
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• 2017

Soybean is an important economical resource of protein and oil for human and animals. The genetic basis of seed protein and oil content has been separately characterized in soybean. However, the genetic relationship between seed protein and oil content remains to be elucidated. In this study, we used a combined analysis of phenotypic correlation and linkage mapping to dissect the relationship between seed protein and oil content. A $F_{10:11}$ RIL population containing 222 lines, derived from the cross between two Korean soybean cultivars Seadanbaek as female and Neulchan as male parent, were used in this experiment. Soybean seed analyzed were harvested in three different experimental environments. A genetic linkage map was constructed with 180K SoyaSNP Chip and QTLs of both traits were analyzed using the software QTL IciMapping. QTL analyses for seed protein and oil content were conducted by composite interval mapping across a genome wide genetic map. This study detected four major QTL for oil content located in chromosome 10, 13, 15 and 16 that explained 13.2-19.8% of the phenotypic variation. In addition, 3 major QTL for protein content were detected in chromosome 10, 11 and 16 that explained 40.8~53.2% of the phenotypic variation. A major QTLs was found to be associated with both seed protein and oil content. A major QTL were mapped to soybean chromosomes 16, which were designated qHPO16. These loci have not been previously reported. Our results reveal a signi cant genetic relationship between seed protein and oil fi content traits. The markers linked closely to these major QTLs may be used for selection of soybean varieties with improved seed protein and oil content.

• 대한수의학회지
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• 제50권3호
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• pp.179-185
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• 2010

The present study was undertaken to establish reference values for the composition blood lymphocyte populations and compare forty three Hanwoo neonatal calves (KC) with twenty one Holstein calves (HC) by blood cell count and immunophynotying. The percentages of CD2+, CD4+, CD8+, CD26+, ACT2+, MHC class, MHC class II and WC1+ T cells, B cells were determined by flow cytometry. The number of lymphocyte and monocyte in HC were higher than those of KC. However, the number of neutrophils was higher in HC than KC. The proportions of CD2+, CD4+, CD8+, MHC class, and WC1+ lymphocytes remained relatively stable during the study period, while there was a moderate increase in the relative percentage of CD26+, ACT2+, MHC class II and B cell from birth to approximately 3 weeks of age. Marked differences in the relative proportions of the lymphocyte subpopulations were noted between the individual calves. The present study shows that the T-cell subpopulations are present in peripheral blood of KC at levels comparable with HC, while the MHC class II and B cell population of KC increases significantly with age. The absolute number of WBC in KC was due to the decrease of absolute number of neutrophil rather than the increase of lymphocyte. The results indicated that KC have significantly higher number of neutrophils, and proportion of MHC class II and B cell than HC.