• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.190.1-190
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.274.2-274
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• 1995

• Journal of Plant Biotechnology
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• v.3 no.1
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• pp.39-44
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• 2001

Plastid transformation was attempted with soybean [Glycine max (L.) Merr.] leaves and photoautotrophic and embryogenic cultures by particle bombardment using the transforming vector pZVII that carries the coding sequences for both subunits of Chlamydomonas reinhardtii Rubisco and a spectinomycin resistance gene (aadA). Spectinomycin resistant calli were selected from the bombarded leaves but the transgene was not present, indicating that the resistance was due to mutations. The Chlamydomonas rbcL and rbcS genes were shown to be site-specifically integrated into the plastid genome of the embryogenic cells with a very low transformation efficiency. None of the transformed embryogenic lines survived the plant regeneration process so no whole plants were recovered. This result does indicate that it should be possible to insert genes into the plastid genome of the important crop soybean if the overall methods are improved.

• The Korean Journal of Ecology
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• v.20 no.4
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• pp.239-244
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• 1997

Hybrid poplar (Populus trichocarpa $\times$ P. deltoides) clones were fumigated with ozone. Fumigation was applied for 6 to 8 hours each day for approximately 3 months at ozone concentrations of 0.090 to 0.115 ppm using by open-top chambers. Growth and biomass of hybrid poplar seedlings were reduced by ozone exposure. Mean percentage of falling leaves in ozone-treated plant was 6 times higher than that of charcoal-filtered plant. Among physiological responses, rate of photosynthesis, stomatal conductance, transpiration and initial Rubisco activity were significantly lower in seedlings grown in ozone environment compared seedlings grown in charcoal-filtered air. All these physiological results supported that biochemical process to be a key feature to understand reduction in photosynthesis.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.63-79
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• 1987

Light regulates a variety of genes in higher plants. The expression of light-induced plant genes is regulated at the level of transcription via red- light photomorphogenic receptor, phytochrome, as well as unknown blue light photoreceptor(s). Ribulose-5-phosphate carboxylase/oxygenase (Rubisco) small subunit (SSB) and light harvesting chlorophyll a/b (Cab) protein are those of the best understood genes regulated by light. 5'-upstream flanking sequence (- -400) of Rubisco SSB and Cab genes sis known as a light responsive, enhance-like element. It responses to red and blue light in transgenic plant system as a tissue specific manner. Phytochrome gene is also regulated by light. In contrast to most of the light regulated plant genes, it is negatively controlled by red light. Search for the cis- and trans-acting factors responsible for the light signal is in progress to understant photomorphogenesis and development in higher plants.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.219.2-220
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• 2000

No Abstract, See Full Text

• Journal of Korean Society of Forest Science
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• v.88 no.2
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• pp.221-228
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• 1999

This study measured the seasonal changes in physiological characteristics and antioxidants of Alnus firma to compare several enzyme activities(Rubisco, Superoxide dismutase(SOD) and Glutathione Reductase(GR)) between resistant and sensitive Alnus firma trees. Resistant and sensitive Alnus firma individuals near Yochon industrial complex were selected to conduct this study in 1997. Photosynthetic capacity, stomatal conductance, transpiration, Rubisco, SOD and GR activities of resistant trees which had no visible damages to air pollution were higher than those of sensitive trees in same area. All physiological results supported that biochemical process to be one of the important key features to understand resistance to air pollution. Increases of photosynthetic capacity and antioxidant enzyme activity in resistant trees in response to air pollution were the results of biological compensation to stress.

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.127.1-127
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• 1998

No Abstract, See Full Text

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.725-728
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• 1996

This study was conducted to investigate effect of leaf temperature on light response and respiration during short-term exposure to a sequnce of PPFD cycle(100-200-300-400$\mu$ mol m$^{-2}$ s$^{-1}$ ) under increasing leaf temperature sequnce up to 3$0^{\circ}C$ and in dark condition, increasing up to 46$^{\circ}C$ with step size (1$^{\circ}C$) in Panax ginseng C. A. Meyer. When leaflet exposed to low light intensity and temperature, Rubisco activity was higher than remained activity in high condition. Leaves adapted to 100$\mu$mol m$^{-2}$ s$^{-1}$ PPFD had a peak response similar to that of 200$\mu$mol m$^{-2}$ s$^{-1}$ at 18$^{\circ}C$, but in above PPFD cycle(300, 400$\mu$mol m$^{-2}$ s$^{-1}$ ) it represented at 17$^{\circ}C$ and 16$^{\circ}C$, re-spectively. $CO_2$ evolution in dark condition increased rapidly when leaf temperature was increased up to 28$^{\circ}C$ and then 'dipped' below steady-state level from above 4$0^{\circ}C$. Thus, Pananx ginseng is able to take advantage of irradiance increase and decrease of $CO_2$ evolution in dark condition to control leaf temperature.mperature.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.1
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• pp.11-17
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• 2004

This study was conducted to determine the relationship of stomatal responses, photosynthesis, and intercellular $CO_2$ concentration( $C_{i}$) of Betula Species to ozone exposure. Five Betula Species(B. costata, B. davurica, B. schmidtii, B. platyphylla var, iaponica and B, ermani) were grown in the greenhouse. One-year-old potted seedlings of the five Betula Species were exposed to ozone(100 pub) for 8 hours da $y^{-1}$ for 5 weeks in a fumigation chamber. Net photosynthesis was significantly different among species and treatments from early in the period of the fumigation. Stomatal conductance and transpiration rate differences among species and treatments became significant after three weeks of fumigation. $C_{i}$ was significantly different only among treatments; $C_{i}$ of four species, except for B. davurica, was higher than that of control plants. Carboxylation efficiency and photo-respiration rate were significantly different among species or treatments; carboxylation efficiency and photo-respiration rate of the five Betula Species were decreased by ozone treatment. It was concluded that stomatal closure of Betula Species may be the result of the reduction of photosynthesis and rubisco activity and the resulting increase of $C_{i}$. The higher $C_{i}$ likely resulted from reduced photosynthesis because of physiological processes.ocesses.