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Potential Detection of γ-Irradiated Panicum miliaceum by Viscosity Measurement during Storage (점도 측정법을 이용한 기장(Panicum miliaceum)의 저장 중 감마선 조사 검지 가능성)

  • 최맑음;권중호;김현구
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.4
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    • pp.531-538
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    • 2003
  • To elucidate the physicochemical change of Panicm miliaceum powders caused by irradiation and identify detection of irradiation, viscosity, TBA value, Hunter color and total viable count were measured after irradiation of Panicum miliaceum powder. Panicum miliaceum powders from Korea and China were irradiated at 0, 2.5, 5, 7.5, 10, 15 kGy using a $Co^{60}$ irradiator and stored for 9 months at $0^{\circ}C$ and $20^{\circ}C$. All samples resulted in decrease for viscosity and specific parameter values by increasing dosage at 50 rpm after irradiation. These values showed dose-dependent relationship (above $r^2$=0.92) between non-irradiated and irradiated samples during storage. These results suggest that the detection of irradiated Panicum miliaceum at various doses is possible by using viscometric method during storage. Total viable count, TBA value and Hunter color were determined as assistant indices for the samples viscosity measured. Total viable count, TBA values showed dose-dependent relationship (2.5 ~15 kGy). The difference in viscosity along with total viable count, TBA values between non-irradiated samples little or some changed with the lapse of post-irradiation time, but was still distinguishable each other more than 6 months at $0^{\circ}C$ and $20^{\circ}C$ for Panicum miliaceum powders from Korea and China.

Clarification of Mixed Fruit and Vegetable Juices Using Various Clarification Methods (혼합과채주스의 청징 및 품질특성 비교)

  • Sohn, Kyung-Suck;Lee, Jun-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.5
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    • pp.629-634
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    • 2006
  • Clarified mixed fruit and vegetable (apple, carrot and orange) juices were prepared using different clarification methods including centrifugation, ultrafiltration (UF), and combined treatment with the blend ratio of 1:1:2, 1:2:1, and 2:1:1 (apple, orange and carrot). Effects of clarification methods on the clarification efficacy as well as other quality parameters were investigated. Clarification was improved with increase in centrifugation speed but was less affected by lowering temperature. Ultrafiltration process was very effective to produce clarified mixed fruit and vegetable juice. Combined treatment did not significantly improve the clarification efficacy since most clarification was already achieved during ultrafiltration process (p>0.05). L*- and b*-values increased while a*-value decreased significantly after clarification regardless of methods in all blend juices (p<0.05). Blend juices with high amount of orange or carrot were better in clarification efficacy and high amount of apple resulted in high turbidity in blend samples.

Physiological and Sensory Characteristics of Demi-glace Sauce with Roux (루(Roux) 첨가에 따른 데미글라스 소스의 이화학적 및 관능적 특성)

  • Kim, Dong-Seok;Choi, Soo-Keun;Lee, Jong-Pil;Choi, Suk-Hyun
    • Culinary science and hospitality research
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    • v.15 no.2
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    • pp.150-160
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    • 2009
  • This study analyzed the quality characteristics of demi-glace sauce with different addition of roux which is usually used as a thickener. The trust level and acceptability for commercial demi-glace sauce were surveyed in order to draw the visual idea and direction for the development of large scale commercial production of demi-glace sauce. Also, the optimal level of salt and roux content in the sauce was determined with various cooking conditions. As the roux content increased, the color became light and the viscosity of the sauce increased while pH, salinity and brix were not affected much by amount of roux. The professional chefs as well as general sensory panel preferred the demi-glace sauce prepared with 9% roux. To sum up, it can be claimed that the optimal conditions established in this study for commercial production of demi-glace sauce ensure the desired quality and economic feasibility.

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Isolation and Characterization of a Bacterium with a Fibrinolytic Activity (Fibrin 용해 균주의 분리 및 특성)

  • 정용준
    • KSBB Journal
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    • v.14 no.1
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    • pp.103-108
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    • 1999
  • A bacterium having strong fibrinolytic activity, S7-16 strain, was isolated from soil. The isolated bacterium was identified and named as Bacillus sp. S7-16. The optimal composition of the medium for the production of fibrinolytic enzyme by Bacillus sp. S7-16 was 0.5%(w/v) polypeptone, 0.5%(w/v) yeast extract, 0.3%(w/v) NaCl, 0.1% (w/v) $KH_2PO_4,\;0.3%(w/v)\;K_2PHO_4,\;and\;0.01%(w/v)\;MgSO_4{\cdot}7H_2O$. The optimal temperature and initial pH of the medium for the production of the enzyme were $35^{\circ}C$ and 7.0, respectively. The maximum production of the fibrinolytic enzyme was obtained after 24 hours of the incubation. Under the above conditions, the culture supernatant had strong fibrinolytic activity. Within pH4~11, the crude fibrinolytic enzyme was stable. The enzyme was stable up to $50^{\circ}C$. The optimum pH and temperature for the enzyme activity were around 7.5 and $40^{\circ}C$, respectively.

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Recovery and Utilization of Proteins and Lipids from Washing Wastewater in Marine Manufacture by Isoelectric Point Precipitation Method 1. The Coagulation Treatment for Washing Wastewatfr of Minced Mackerel Meat (수산가공공장 폐액의 등전점 침전처리에 의한 유용성분 재회수 이용 1. 고등어 육 고기풀 제조시 발생되는 폐액의 처리장치 개발)

  • 서재수;조순영
    • KSBB Journal
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    • v.10 no.1
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    • pp.1-8
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    • 1995
  • A lot of water soluble proteins and lipids are released from minced mackerel meat and lost into the washing waste during the leaching process of Kamaboko or surimi manufacture. The removed proteins and lipids are not only an edible things but also a big burden for treating the wastewater. In order to recover the proteins from the effluent and to use as food stuff, the "pH-shifting" treatment, a modified isoelectric point precipitation method, was tried. This method is based on a myogen-aggregation phenomenon, which occurs when a solution of sarcoplasmic proteins is acidified or alkalified beyond the critical pH zone of 2∼3 or 12∼13 respectively and then neutralized. The maximum amount of precipitation was obtained by shifting the pH of the wastewater from original pH to isoelectric point (pH 4) or alkali pH 12 and then changing to neutral pH. The precipitates were easily collected by filteration or centrifuging at 10,000rpm. The oils which were only floating in the washing wastewater are easily recovered by seperating with oil separator after pouring. The recovered proteins were slightly denaturated during this pH shifting precipitation process, while the composition of amino acids was good balance as a food.

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A High Viscosity of Curdlan at Alkaline pH Increases Segregational Resistance of Concrete (염기성 pH에서의 고점도 커들란에 의한 콘크리트의 재료분리 억제 효과 증진)

  • 이인영;김선원;이중헌;김미경;조인성;박영훈
    • KSBB Journal
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    • v.14 no.1
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    • pp.114-118
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    • 1999
  • In order to use a polysaccharide, curdlan, as a concrete admixture, we first developed a pilot-scale fermentation process for the mass production of curdlan. We also examined the rheological properties of curdlan, and tested how well the curdlan obtained in this work increased the segregational resistance of the cement slurry. Fermentation was performed in a 300-liter fermenter equipped with 3 disk-turbine impellers. Since curdlan production is stimulated under nitrogen-limiting conditions, the culture pH was shifted from the optimal pH for cell growth (pH 7.0) to the optimal pH for curdlan production (pH 5.5) at the onset of ammonium exhaustion. We obtained a curdlan production of 65 g/L in 120 hr batch cultivation of Agrobacterium species. The insoluble curdlan at the final stage of fermentation was readily harvested by centrifugation together with the cells. The freeze-dried sample contained 78% (w/w) of curdlan. The solubility and viscosity of the curdlan increased with the increase of the solution pH, which enhances the viscosity of concrete since the pH of concrete is extremely high (pH 13.0). Test results of the curdlan as a concrete admixture with cement slurry demonstrated that it prohibits the leakage of water. In conclusion, this work certifies and enlarges curdlan's industrial potential as a concrete admixture.

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Characterization of Low-Trans Solid Fat from Canola and Fully Hydrogenated Soybean Oil by Lipase-Catalyzed Interesterification Reaction (효소적 에스테르 교환 반응 시 카놀라유와 대두극도경화유의 비율에 따른 저트랜스 고체지방의 특성)

  • Kim, Young-Joo;Lyu, Hyun-Kyeong;Lee, Seon-Mo;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.9
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    • pp.1320-1327
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    • 2010
  • Lipase-catalyzed interesterification of canola (CO) and fully hydrogenated soybean oil (FHSBO) at different weight ratios (70:30, 75:25, and 80:20) was performed in a batch type reactor to produce low-trans solid fats. Each reaction was conducted in the shaking water bath for various reaction times (1, 3, 6, 18 and 24 hr) at 70oC and 220 rpm using Lipozyme TLIM (20 wt% of total substrate) from Thermomyces lanuginosus. After 24 hr reaction, solid fat content (SFC) by differential scanning calorimetry (DSC), fatty acid and triacylglycerol (TAG) composition of low-trans solid fats were determined. SFC of the products was reduced when the content of canola oil in the reaction mixture was increased. Major fatty acids were stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2). Trans fatty acid content in the low-trans solid fats showed less than 0.3 wt%. In the HPLC analysis, major TAG species showed LOO (linoleyl-oleoyl-oleoyl), OOO, POO/SOL, SOO, and SOS.

Effect of Grinding on Solubility and Particle Size of Pefioxacin by Planetary Ball-Mill (유성볼밀을 이용한 난용성 Pefloxacin의 분쇄조작에 의한 입자 설계)

  • 임영근;김진우;최우식;야마모토;정해영
    • Journal of Life Science
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    • v.9 no.2
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    • pp.194-200
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    • 1999
  • Grinding aid is a necessary unit operation when the raw materials are handled in solid form, and the purpose is to improve the bioavailability by reducing the particle size. For the particle design of pefloxacin, the dry planetary ball-mill was used. With the drying process, 330 g of zirconia ball with its size of 2 mm in diameter and 10 g of pefloxacin were transferred to the pot and mixed well. The mixture was ground at 112 rpm (60 Hz) for 15, 30, and 60 min, respectively. The most satisfactory grinding products were generated at 15 min of grinding time for their particle size. The volume mean diameter $\X_50$ of the grinding products was 2.97 $\mu$m. X-ray diffraction (XRD), differential scanning calorimeter (DSC), and infrared spectroscopy (IR) patterns were relatively unchanged before pulverizing pefloxacin and in the progress of grinding. Thus, these results suggest that this pulverizing method can be used for grinding products without evident effect on stability of the drug pefloxacin. Dissolution test was carried out to set up the optimal detective condition against residual antibacteria of fish by HPLC. The grinding pefloxacin for 15 min is most effective in dissolution test.

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Study on the Improvement of Milling Recovery and Performance(II) -Experimental Study on Rice Whitening Mechanism- (도정수율(搗精收率)과 성능향상(性能向上)을 위(爲)한 연구(硏究)(II) -벼의 정백과정(精白過程)에 대(對)한 실험적(實驗的) 연구(硏究)-)

  • Park, Joon Gul;Chung, Chang Joo;Noh, Sang Ha
    • Journal of Biosystems Engineering
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    • v.7 no.1
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    • pp.62-72
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    • 1982
  • 이 연구(硏究)는 정백과세중(精白過稅中)에 발생(發生)하는 도정산물(搗精産物) 즉, 정백미(精白米), 미당(米糖), 쇠미등(碎米等)의 발생(發生)을 조사(調査)함으로써 현미(玄米)의 정백(精白)이 진행(進行)되는 과정(過程)을 밝히고자 함이다. 본(本) 실험(實驗)에서는 분풍마찰식(噴風摩擦式) 정미기(精米機)를 사용(使用)하였으며, 정미기(精米機)의 롤러 회전속도(回轉速度)를 850rpm으로 고정(固定)하고 출구저항(出口抵抗)은 3가지 수준(水準)으로 변화(變化)시켰다. 한편 이 실험(實驗)에 사용(使用)된 벼는 밀양 23호(號)로써 전형적인 통일계품종(品種)의 일종(一種)이다. 매순환(每循環)마다 생산(生産)되는 정백미(精白米) 및 부산물(副産物)로부터 sample를 채취(採取)하여 미당(米糖), 대소쇄미(大小碎米), 완전미(完全米) 및 도정수율(搗精收率)을 분석(分析)하였다. 실험결과(實驗結果)를 요약(要約)하면 다음과 같다. 1) 전체당층(全體糖層)의 75%가 2번째 순환이내(循環以內)에서 제거(除去)되었다. 당층제거율(糖層除去率)은 출구저항(出口抵抗)에 크게 영향(影響)을 받으며, 높은 출구저항(出口抵抗)에서 높은 당층제거율(糖層除去率)을 보였다. 2) 대쇄미(大碎米)의 발생(發生)은 대부분 정백과정(精白過程) 초기단계(初期段階)에서 이루어 졌으며, 완전미(完全米)로 부터의 새로운 쇄미발생(碎米發生)은 순환(循環)이 반복(反復)됨에 따라서 급격(急激)히 감소(減少)하였다. 출구저항(出口抵抗)과의 관계(關係)는 출구저항(出口抵抗)이 증가(增加)할수록 대쇄미(大碎米)의 발생(發生)도 증가(增加)하였다. 3) 미당집적기(米糖集積器)로부터 채취(採取)한 sample에서 측정(測定)한 소쇄미(小碎米)는 양적(量的)으로 다른 도정산물(搗精産物)에 비(比)해 적은 편이었다. 8%의 미당(米糖)이 제거(除去)된 후(後) 소쇄미(小碎米)는 현미중량(玄米重量)의 0.6% 이하(以下)로 나타났다. 4) 도정수율(搗精收率)과 완전미수율(完全未收率)은 어느 정도 출구저항(出口抵抗)의 영향(影響)을 받는 것으로 나타났다. 출구저항(出口抵抗)이 증가(增加)함에 따라서 특히 완전미수율(完全未收率)은 감소(減少)하는 경향(傾向)을 나타냈기 때문에 백미(白米)의 질적저하(質的低下)를 막기 위해 높은 출구저항(出口抵抗)(본(本) 실험결과(實驗結果)에 의(依)하면 85g/$cm^2$)을 사용(使用)하는 것은 부적당(不適當)한 것으로 사려(思慮)된다. 5) 백도계(百度計)의 측정치(測定値)와 미당제거정도(米糖除去程度)(정백도(精白度)) 사이에는 고도(高度)의 상관관계(相關關係)가 있었다. 그러므로 "KETT"제(製) 백도계(百度計)는 정백미(精白米)의 정백도(精白度)를 판명(判明)하는데 사리(使利)하게 사용(使用)될 수 있다.

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Technical Feasibility of Ethanol-Kerosene Blends for Farm Kerosene Engines (에타놀-석유(石油) 혼합연료(混合燃料)의 농용석유(農用石油)엔진에의 이용(利用)에 관(關)한 연구(硏究))

  • Bae, Yeong Hwan;Ryu, Kwan Hee
    • Journal of Biosystems Engineering
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    • v.7 no.1
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    • pp.53-61
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    • 1982
  • As an attempt to reduce the consumption of petroleum resources and to improve the performance of a kerosene engine, a series of experiments was conducted using several kinds of ethanol-kerosene blends under the various compression ratios. The engine used in this study was a single-cylinder, four-cycle kerosene engine having a compression ratio of 4.5. To investigate the feasibility of ethanol-kerosene blends in the original engine, kerosene and blends of 5-percent, 10-percent, and 20-percent-ethanol, by volume, with kerosene were used. And to investigate the feasibility of improving the performance of the kerosene engine, a portion of the cylinder head was cut off to increase the compression ratio up to 5.0 by reducing the combustion chamber volume. Kerosene and blends of 30-percent and 40-percent-ethanol, by volume, with kerosene were used for the modified engine with an increased compression ratio. Variable speed tests at wide-open throttle were also conducted at five speed levels in the range of 1000 to 2200 rpm for each compression ratio and fuel type. Volumetric efficiency, engine torque, and brake specific fuel consumption were determined, and brake thermal efficiency based on the lower heating values of kerosene and ethanol was calculated. The results obtained in the study are summarized as follows: A. Test with the original engine: (1) No abnormal conditions were found when burning ethanol-kerosene blends in the original engine. (2) Volumetric efficiency increased with ethanol concentration in blends. When burning blends of 5-percent, 10-percent, and 20-percent ethanol, by volume, with kerosene, average volumetric efficiency increased 1.6 percent, 2.6 percent, and 4.1 percent respectively, than when burning kerosene. (3) Mean engine torque increased 5.2 percent for 5-percent-ethanol blend, 9.3 percent for 10-percent-ethanol blend, and 11.5 percent for 20-percent-ethanol blend than for kerosene. Increase in engine torque when using ethanol-kerosene blends was due to the improved combustion characteristics of ethanol as well as an increase in volumetric efficiency. (4) Up to ethanol concentration of 20 percent, mean brake specific fuel consumption was nearly constant inspite of the difference in heating value between ethanol and kerosene. (5) Brake thermal efficiency increased 0.3 percent for 5-percent-ethanol blend, 3.8 percent for 10-percent-ethanol blend, and 6.8 percent for 20-percent-ethanol blend than for kerosene. B. Test with the modified engine with an increased compression ratio: (1) When burning kerosene, mean volumetric efficiency, engine torque, and brake thermal efficiency were somewhat lower than for the original engine. (2) Engine torque increased 15.1 percent for 30-percent-ethanol blend and 18.4 percent for 40-percent-ethanol blend than for kerosene. (3) There was no significant difference in brake specific fuel consumption regardless of ethanol concentration in blends. (4) Brake thermal efficiency increased 15.0 percent for 30-percent-ethanol blend and 19. 5 percent for 40-percent-ethanol blend than for kerosene.

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