• 수도
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• 통권38호
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• pp.36-39
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• 1987

• Journal of Microbiology
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• 제41권2호
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• pp.73-77
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• 2003

Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were identified as B. vallismortis, B. subtilis, Aeromonas hydrophila and B. amyioliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses. The optimal proteolytic activity was observed in the temperature and pH ranges of 40-70$^{\circ}C$ and 8.0-8.5, respectively. The proteolytic activities of all the isolates were partially inhibited by phenylmethylsulfonylfluoride (PMSF), and the isolates Ni 26, Ni 39 and Ni 49 were inhibited by the metalloprotease inhibitor, 1,10-phenanthroline. Zymographic analyses of the culture supernatants revealed the presence of at least two pretenses in all isolates.

• 한국물환경학회지
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• 제29권1호
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• pp.1-6
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• 2013

This study was focused on improving nitrification efficiencies of M-dephanox (Modified-Dephanox) process. Rotating biological contactor (RBC) was used instead of floating sponge type media in nitrification reactor. High ammonia removal efficiencies were observed in nitrification reactor, regardless of organic loading from contactor of M-dephanox process. Denitrification efficiencies were also increased to maintain low $NO_3-N$ concentration in effluent. This enhanced phosphate release in anaerobic contactor and resulted in high removal efficiencies of phophorus. Average removal efficiencies of $TCOD_{Cr}$ and $SCOD_{Cr}$ were 93.8% and 81.6%, respectively, while those of TKN and ${NH_4}^+-N$ were 80.9% and 74.4%, respectively. As for phosphorous treatment, the average removal efficiencies of TP and OP were 94.7% and 94.3%, respectively. Also, effect of operating temperature on nitrogen removal was examined. Average removal efficiency of TN was 65.8 % at $15^{\circ}C$ or below (at average temperature of $13.3^{\circ}C$), while that was 82.8% at $15^{\circ}C$ or above (at average temperature of $21.9^{\circ}C$).

• 한국물환경학회지
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• 제18권2호
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• pp.189-199
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• 2002

Nitrogen, in its various forms, can deplete dissolved oxygen levels in receiving waters, stimulate aquatic growth, exhibit toxicity toward aquatic life and affect the suitability of sewage for reuse. Pilot-scale Rotating Biological Contactor(RBC) experiments were conducted to examine biological nitrification, respectively, of municipal sewage with five different internal recirculation ratios of 0, 1, 2, 3, and 4 using the constant hydraulic loading of $205L/m^2{\cdot}day$. The use of internal recirculation improved nitrification on account of the dilution of biodegradable organic carbon in influent sewage down to 15 mg/L of $SBOD_5$ or less. Ammonium nitrogen of $14.3{\pm}2.4%$ was consumed by cellular assimilation without the occurrence of denitrification. The thickness of biofilm didn't seem effect significantly the nitrification and denitrification. Nitrification with internal recirculation was found to occur using hydraulic loading rate of as high as $205L/m^2{\cdot}day$, which was beyond the generally known values of it.

• 한국물환경학회지
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• 제20권2호
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• pp.190-195
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• 2004

A new technology for advanced wastewater treatment was developed using a modified Rotating Biological Contactor (RBC) process, named as Rotating Activated Bacillus Contactor (RABC) process that utilizes Bacillus sp., the facultatively anaerobic or activated microaerophilic bacteria on multiple-stage reticular rotating carriers, as a predominant species. The RABC process for a municipal wastewater with relatively low concentrations of organics, nitrogen, and phosphorus showed stable and high removal efficiencies, less than $BOD_5$ 10 mg/L, T-N 15 mg/L, and T-P 1.5 mg/L in final effluent. The performance load of RABC process was shown to be $1.23kg{\cdot}BOD/m^2{\cdot}day$ for the first stage (average $0.31kg{\cdot}BOD/m^2{\cdot}day$ for the total stages) based on both removed BOD and converted disc area corresponding to the reticular one. The sludge produced in the RABC process is characterized by low generation rate (about $0.18kg{\cdot}MLSS/kg{\cdot}BOD$) and excellent settleability. The number ratio of Bacillus ($2.4{\times}10^6CFU/ml$) to heterotrophic bacteria ($3.6{\times}10^7CFU/ml$) inhabiting in the biofilms of the RABC process was 6.7 %, indicating that Bacillus sp. was a predominant species in the biofilms. The RABC process with reticular rotating carriers showed its excellent performance for the advanced wastewater treatment without any offensive odor problem due to organic overloading.

• 미생물학회지
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• 제28권4호
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• pp.337-344
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• 1990

미국 뉴저지주에 있는 10개의 회전원판 처리장에서 생물막 시료를 채취하여 사상세균에 대해 현미경적 연구를 실시하였다. 활성오니에서 부유형 성장을 하며 흔히 출현하는 Type 1701, Type 0041. Type 021N, Nocardia, Beggiatoa 및 Sphaerotilus가 회전원판체 생물막에서도 출현하였으나 수도(abundance)는 크게 달랐다. 여기서는 Beggiatoa의 출현빈도가 가장 높았으며, Sphaerotilus, Type 0041, Type 1701, Type 021N, Nocardia의 순이었다. 회전원판 생물막에서 발견된 Beggiatoa는 형태적으로 뚜렷히 구별되는 2가지 Type이 존재하였다. 회전원판체의 중심축으로 100cm 깊이에 존재하는 생물막과 외부의 생물막에 존재하는 사상세균의 군집에 대해 인자분서법에 의한 통계처리를 수행한 결과 대부분의 경우, 내부의 군집과 외부의 군집이 통계적으로 유의하게 달랐으며 장소(내부, 외부)와 단(stage) 사이에도 많은 경우 통계적으로 유의한 상호작용이 있었다.

• 한국양식학회지
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• 제6권4호
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• pp.311-321
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• 1993

Ammonia accumulation is regarded as the limiting factor of the first priority in water qualities of aquatic culture systems. Nitrification efficiency and characteristics in seawater were evaluated using Rotating Biological Contactor (RBC) process as a part of the recycling water treatment facilities for marine fish culture system. Ammonia removal efficiency regarded 99.7 to $83.7\%$ at the ammonia surface loading rates of 48 to $393 mg/m^2$ -day. RBC process was able to withstand to the fluctuation of influent ammonia concentrations and loading and produced the stable effluent. The mathematical model on the fixed-film biological reactor developed by Kornegay seemed to be suitable to RBC process kinetic evaluation for the recycling water treatment of the marine fish culture system. Area capacity constant (P) and half-velocity constant (Ks) in the model were 0.188g/m^2$-day and 1.25mg/l, respectively.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.95.1-95
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• 1999

No Abstract, See Full Text

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.243-246
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• 2002

In this study, biological production of fumaric acid by Rhizopus oryzae KCTC 6946 using rotary biofilm contactor was investigated. In study of neutralizing agent on fumaric acid production, $Na_2CO_3$ was more effective than NaOH. After 24 hr of incubation with a rotating speed of 10 rpm at $35^{\circ}C$, biofilm was grown on and around the surface of the disks. The yield and volumetric productivity of rotary biofilm contactor were 33.8% and 0.595 g/L${\cdot}$h, respectively, with the optimum effective disk surface area of 1,583 $cm^2/L$.

• 한국수산과학회지
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• 제35권1호
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• pp.21-26
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• 2002

Package 형태로 설계하고 제작된 pilot scale 순환여과식 양식시스템에 틸라피아를 $2\%$의 사육밀도로 수용하여 사육조의 각종 수질인자의 변화를 측정하고 회전원판 반응기, floating bead filter, 포말분리기 등의 순환수 처리효율을 검토하여 순환여과식 양식시스템의 개시운전시 발생하는 현상을 고찰하고 순환수 처리장치의 안정화에 소요되는 시간을 측정하고자 하였다. 평균 어체중 392.8g의 나일 틸라피아 173마리를 수용하여 일간 보충수를 사육조 용적의 $10\%$를 사용하여 14일간 어류사육을 실시하여 생물학적 순환수 처리장치인 회전 원판 반응기의 효율 변화를 검토하였다. 암모니아성 질소는 사료 투입 직후부터 증가하여 7일 후 부터는 $0.3gm^3$ 정도의 농도를 보였다. 사료 투입 후 3일이 경과하면서부터 아질산성 질소, 7일 이후부터 질산성 질소가 증가하였으며 12일이 경과하면서 시스템의 아질산성 질소의 농도가 감소하여 회전원판 반응기가 숙성되었다 총 무기질소는 사료 투입 후 10일이 경과하면서 $30g/m^3$ 정도의 일정한 농도를 유지하였다. 사육조 내의 용존 산소농도는 $4{\~}5g/m^3$의 안정된 농도를 유지하였으며 pH와 총알칼리도는 질산화 반응기의 숙성에 따라 어류생장에 영향을 미치지 않는 범위에서 감소하였다 총 부유 고형물과 화학적 산소 요구량은 보충수와 거의 동일한 수질을 유지할 수 있었다. 회전원판 반응기는 암모니아 제거와 동시에 용존산소 폭기 효율도 나타내었고 floating bead filter는 고형물 제거뿐 아니라 질산화에도 뛰어난 효율을 보였으며 회전 원판 반응기는 사료 투입 후 2일, floating bead filter는 4일째부터 암모니아 제거가 시작되었다.