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Studies on the Antioxidant and Whitening Effects of Chamaecyparis obtusa Extract (편백나무(Chamaecyparis obtusa) 추출물의 항산화, 미백효과에 관한 연구)

  • Joung, Yeo-Won;Kim, Yu-mi;Jang, Young-Ah
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.6
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    • pp.1496-1506
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    • 2020
  • This study was conducted as follows in order to find out the possibility of using (Chamaecyparis obtusa) by part as a cosmetic material. Chamaecyparis obtusa was separated leaves, bark, wood, and root, and extracted with 99.9% ethanol, and the antioxidant and whitening effects of the sample were confirmed. For this, the following studies were conducted. Antioxidant evaluation was confirmed by 1 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+) radical scavenging activity. To evaluate the whitening effect, mushroom tyrosinase inhibitory activity evaluation and cytotoxicity evaluation of Chamaecyparis obtusa extract through MTT assay were conducted, and cellular tyrosinase inhibition rate was measured and melanin contents was confirmed. As a result of antioxidant activity, Bark extract among Chamaecyparis obtusa parts showed the best efficacy, and bark in B16F10 melanoma cell showed tyrosinase activity inhibition and melanin contents inhibitory effects. Therefore, it was confirmed that the ethanol extract of Chamaecyparis obtusa was developed as a natural material for safe and excellent whitening functional cosmetics.

Effect of Saline Soil and Crop Growth with Bottom Ash from Biomass Power Plant Based Wood Pellet (우드펠릿 기반 바이오매스 발전소로부터 배출된 저회를 활용한 염류토양 및 작물성장에 미치는 영향)

  • So-Hui Kim;Seung-Gyu Lee;Jin-Ju Yun;Jae-Hyuk Park;Se-Won Kang;Ju-Sik Cho
    • Korean Journal of Environmental Agriculture
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    • v.41 no.4
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    • pp.310-317
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    • 2022
  • BACKGROUND: The salt in soil interrupts crop growth. Therefore, water resources are used to remove any salt found in the soil. However, water resources have been reduced by global warming; thus, a new study is required into reducing the salt in soil. Recently, the bottom ash (BA) of a biomass power plant was found to be similar to biochar. Hence, it can be used to remove heavy metals and wastewater through the adsorption characteristics of BA. The objective of this study was to evaluate the improvement effects on crop growth in saline soil containing the BA from biomass power plants. METHODS AND RESULTS: The effect on crop growth in the saline soil supplemented with BA was studied with the crop-planted pots, which were packed by reclaimed greenhouse soils collected from Byolyang, Suncheon. The BA application level was 25, 50, 100, 200, and 400 kg/10a (referred as BA25, BA50, BA100, BA200, and BA400, respectively). The BA increased the fresh weights of the leaf and root, while nitrogen uptake increased by approximately 24-102% and 54-77%, respectively for the lead and root. The phosphorous uptake increased by 38%, although only in the leaf of the lettuce. In the case of soil, BA increased water content, pH, EC, CEC, and NH4+ and the SAR of the soil decreased by 5-15%. The bottom ash increased the contents of Ca2+ and Mg2+, and fixed the amount of Na+. CONCLUSION(S): It was confirmed the bottom ash of a biomass power plant, based on wood pellets, improved crop growth, and increased the nutrient uptake of crops in saline soil. In addition, bottom ash, which has a wide range of porosity and high values of pH and EC, improved properties of the saline soil. However, the BA has a large amount of B, As, and heavy metals. Finally, it may require a study on the safety and contamination of heavy metals contained in the bottom ash, which would be applied in soil for a long time.

Eeffect of Wood Xylem Flour in Liquid Culture on Mycelial Biomass of Lentinus lepideus and Lentinus edodes (목분 첨가 액체배양에 의한 잣버섯 및 표고 균사배양의 촉진 효과)

  • Lee, Wi-Young;Ahn, Jin-Kwon;Park, Eung-Jun;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.36 no.1
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    • pp.45-50
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    • 2008
  • This study was carried out to investigate the promoting effect of wood flour on the mycelial growth of Lentinus lepideus and Lentinus edodes. To determine the optimal culture condition, we first examined the tissue origin of pine flour (Pinus densiflora) including needle, bark, root and xylem. Only the xylem-derived flour increased mycelial growth compared to no treatment control. The addition of the xylem flour (5 g/l) showed the highest increase and the glucose level in the basal medium was best at 10 g/l. The smaller particle size of the xylem flour showed the positive effect on mycelial growth; two-fold increase when supplemented with flour of which particle size is less than $106\;{\mu}m$ in diameter compared to $425\;{\mu}m$. The addition of the xylem flour continuously increased the mycelial production for 25 days while mycelia stopped growing within 15 days without the xylem flour. In addition, when woody flour obtained from the different tree species was applied to L. edodes mycelial culture, all treatments accelerated mycelial production compared to the control. Based on all results described above, we conclude that the supplementation of woody flour to culture medium may be an another promising way to increase mycelial production of economically important fungi.

Selection of Ectomycorrhizal Isolates of Tricholoma matsutake and T. magnivelare for Inoculation on Seedlings of Pinus densiflora In Vitro (소나무 유묘에서 송이 외생균근 형성 균주의 선발)

  • Ka, Kang-Hyeon;Park, Hyun;Hur, Tae-Chul;Bak, Won-Chull
    • The Korean Journal of Mycology
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    • v.36 no.2
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    • pp.148-152
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    • 2008
  • We inoculated hypal suspension of Tricholoma matsutake and T. magnivelare were examined on Pinus densiflora seedlings grown in a granite soil substrate with 1/2 PDMP (12 g/l potato dextrose broth, 1.5 g/l malt extract, and 0.5 g/l peptone) medium. Four months after inoculation, the pine seedlings were examined for infection rate, matsutake aroma, and Hartig-net formation. The roots of pine seedling formed ectomycorrhizal roots in the 9 isolates from 12 isolates of T. matsutake and T. magnivelare. However, the seedlings showed different ectomycorrhizae forming rates among the 9 isolates. While matsutake aroma was confirmed from the ectomycorrhizal seedlings, the pine seedling contaminated by bacteria or fungi did not form matsutake ectomycorrhizae with sickening smell. Thus, the aroma was chosen as a good way for the verification of mycorrhizal infection. At the early stage, the mycorrhizal roots showed unramified and branched types without root hair. They also showed thin mantle layers, Hartig-nets, and turned into black color at later stage. Among the examined strains, that of Yecheon isolated in 1995 showed the best infection rate, which indicated that we need to pay attention to the selection of isolates for better result.

Mesquite (Prosopis juliflora) Pods as a Feed Resource for Livestock - A Review -

  • Sawal, R.K.;Ratan, Ram;Yadav, S.B.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.5
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    • pp.719-725
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    • 2004
  • Mesquite or Vilayati babul (Prosopis juliflora) is a drought resistant, evergreen, spiny tree with drooping branches and a deep laterally spreading root system. It grows in semi-arid and arid tracts of tropical and sub-tropical regions of the world and is spreading because the leaves are unpalatable and animals do not digest its seed. The mesquite has become a major nuisance; cutting or pruning its branches to form a canopy would provide shade for travelers, aid harvesting of pods, as well as make available wood for fuel. An average plant starts fruiting by 3-4 years of age and yields annually 10-50 kg pods/ tree, which can be collected from May-June and September-October. Availability of pods worldwide is estimated to be about 2-4 million metric tonnes. Ripe pods are highly palatable; on dry matter basis they contain 12% crude protein, 15% free sugar, a moderate level of digestible crude protein (7% DCP) with a high level of energy (75% TDN). The pods contain low tannin levels below those toxic to animals. Seeds contain 31-37% protein; pods should be finely ground before feeding to facilitate utilization of the seeds. Mesquite pods could replace costlier feed ingredients such as grain and bran contributing 10-50% of the diet. Phosphorus supplements need to be added when mesquite pod, exceeds 20% of animals' diet.

The Scavenging Effect of 207 Oriental Medicines and 19 Oriental Prescriptions on 1,1-Diphenyl-2-Picrylhydrazyl Radical (207종 한약과 활성 한약이 포함된 19종 한약 방제의 1,1-Diphenyl-2-Picrylhydrazyl 라디칼 소거작용)

  • Kim Min-San;Her Jong-Moon;Park Jong-Cheol
    • Herbal Formula Science
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    • v.12 no.1
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    • pp.159-175
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    • 2004
  • The inhibitory effects of 207 oriental medicines and 19 oriental prescriptions on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were examined. The most effective oriental medicines were the fruit of Terminalia chebula, followed in order by the seed of Areca catechu, the heart wood of Caesalpinia sappan, the pericarp of Castanea crenata, the seed of Nelumbo nucifera, Rhus galls, the root of Sanguisorba officinalis, and the bark of Betula platyphylla at the concentration of 10 ${\mu}g$/mL. Of the 19 oriental prescriptions, Buyeonsan and Samsinhwan showed the potent scavenging activities of 88% and 71% on DPPH radical at the concentration of 5 ${\mu}g$/mL, respectively.

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A Study on the Dyeing Properties of Yellow and Red Natural Dyes(1) (황색 및 적색계 천연염료의 염색성에 관한 연구(1))

  • Shin, Young-Joon
    • Journal of the Korea Fashion and Costume Design Association
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    • v.17 no.2
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    • pp.45-61
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    • 2015
  • In order to analysis on color difference of natural dyes, I have dyed Hanji, cotton, silk fabric and exposed them to carbon arc light. The results of experiment have been analysed by wavelength of maximum absorption, amounts of dye uptake, color difference, Hunter's value and Munsell's value. Gardenia Jasminoides is monogenetic dye, but it gained darker color by pre-mordanting method used Al mordant agent and greenish yellow color by Fe mordant agent. whereas Curcuma, an Amur cork, and bud of pagoda tree are shown as greenish yellow color, and A barberry root, Betel nut, and Rhubarb are shown as reddish yellow color. these gained khaki colored dyed fabric by Fe mordanting. In addition, Sappan wood showed great result in pre-mordanting. Especially, it gained dark red color by Al pre-mordanting. The pink color was shown by post-mordanting. and Logwood showed great dyeing result in Hanji and cotton better than silk. Specially pre-mordanting was effective. Hanji and cotton showed greenish blue color by Al pre-mordanting, and silk showed brown color.

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A Study on the Dyeing Properties of Yellow and Red Natural Dyes(2) (황색 및 적색계 천연염료의 염색성에 관한 연구(2))

  • Shin, Young-Joon
    • Journal of the Korea Fashion and Costume Design Association
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    • v.18 no.4
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    • pp.77-86
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    • 2016
  • In order to analysis on color difference of natural dyes, I have dyed Hanji, cotton, silk fabric and exposed them to carbon arc light. The results of experiment have been analysed by Munsell's value, and compared the difference of colors which were recognized visually. Gardenia Jasminoides is monogenetic dye, but it gained darker color by pre-mordanting method used aluminum mordant agent and greenish yellow color by ferric mordant agent. whereas Curcuma, an Amur cork, and bud of pagoda tree are shown as greenish yellow color, and A barberry root, Betel nut, and Rhubarb are shown as reddish yellow color. these gained khaki colored dyed fabric by ferric mordanting. In addition, Sappan wood showed great result in pre-mordanting. Especially, it gained dark red color by aluminum pre-mordanting. The pink color was shown by post-mordanting. and Logwood showed great dyeing result in Hanji and cotton better than silk. Specially pre-mordanting was effective. Hanji and cotton showed greenish blue color by aluminum pre-mordanting, and silk showed brown color. However the color, which was recognized visually, differed from colorimeter sometimes. Therefore, such color table might be necessary for the natural dyeing.

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Development of multipurpose seed paper from waste paper(II) - Focused on field test of manufactured seed paper - (폐지를 이용한 기능성 육묘지의 제조(제2보) - 육묘지 적성 시험 -)

  • Eom, Tae-Jin;Park, Soung-Bae
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.39 no.1 s.119
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    • pp.30-37
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    • 2007
  • The seed paper was used in farm field recently for a sound young plant. The most of seed paper are made of synthetic non-woven sheet. Therefore, it is very difficult to bio-degrade in soil and is very hard to have some special function, for example keeping herbicide and/or insecticide activity because of its lack of chemical acceptability. The purpose of this research is manufacture of seedling paper which have a function of herbicide activity from waste paper. The fiber properties from waste paper were remarkably improved by fine removal with washing and/or flotation process. The paper-making ability for seed paper was enhanced with enzyme treatment of secondary fibers. The paper for seedling must have a good bio-degradation ability in soils. The absorption amount of chemical like as dithiopyr was increased remarkably in enzyme treated base paper. The embossing treatment of base paper was very effective for seed attachment and chemicals retention. And also, the developed seed paper showed a good penetration property of young root through embossed paper.

Prevention of Oak Wilt by Tree Injection of Culture Suspension of an Antifungal Microorganism, Streptomyces blastmyceticus against Oak Wilt Fungus, Raffaelea quercus-mongolicae

  • Lee, Jin Heung;Hong, A Reum;Yun, Ji Ho;Seo, Sang Tae;Lee, Jong Kyu
    • Journal of Forest and Environmental Science
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    • v.34 no.5
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    • pp.376-381
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    • 2018
  • For the control of oak wilt caused by Raffaelea quercus-mongolicae, an antifungal microorganism, Streptomyces blastmyceticus, was used as a potential agent. Culture suspension of S. blastmyceticus was injected into Quercus mongolicae in the research forest of Kangwon National University by $ChemJet^{(R)}$ trunk injection and Macro-infusion at root flare injection. $Alamo^{(R)}$ (a.i., propiconazole 14.5%), a fungicide currently used for the control of oak wilt in USA, was also treated by both methods to compare the efficacy. For preventive efficacy, culture suspension of the pathogen was inoculated at 1 month after injection of either agent. Tested trees were cut at 3 months after treatment, stained with 1% Fuchsin acid, and then non-conductive area (NCA) and re-isolation frequency (RIF) of oak wilt fungus were compared among treatments. While NCA was the highest as 47.3% in pathogen only treatment, it was the lowest as 16.0% in sterilized water treatment by Macro-infusion. NCAs of Alamo treatment by Macro-infusion and ChemJet injection were 25.3% and 32.1%, respectively. NCA of S.blastmyceticus treatment by ChemJet injection was 32.3%, similar with Alamo treatment's by ChemJet injection. All treatments by either injection method showed significantly lower NCA compared to the pathogen only treatment. These results indicate that S. blastmyceticus injection shows the preventive efficacy against oak wilt disease by suppressing the growth of pathogen injected. NCA of Macro-infusion injection of sterilized water was lower as 16.0%, compared to 21.3% of ChemJet injection. It means that Macro-infusion is more effective in translocation of sterilized water than ChemJet injection by even distribution. RIF from wood discs of treated trees showed high in pathogen only treatment, but relatively low in S. blastmyceticus treatment. RIF results were correlated with NCA results. From the above results, it was confirmed that S. blastmyceticus showed preventive efficacy against oak wilt disease by ChemJet trunk or Macro-infusion at root flare injection.