• 제목/요약/키워드: root to shoot ratio

검색결과 192건 처리시간 0.022초

Efficient Micropropagation of Pear Germplasm Using Soot Tips and Nodal Explants

  • Yi, JungYoon;Lee, GiAn;Chung, JongWook;Lee, YoungYi;Gwag, JaeGyun;Lee, SeokYoung
    • 한국자원식물학회지
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    • 제28권6호
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    • pp.690-696
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    • 2015
  • We micropropagated pear (Pyrus species) using shoot tips and nodal explants from three pear genotypes. The ability to establish shoot tip cultures, proliferate shoots, induce rooting, and acclimatize the resulting plantlets are all elements of in vitro micropropagation. Shoots were induced from shoot tips on Murashige and Skoog medium (MS) with five different plant growth regulator combinations. The highest shoot formation rates were achieved for the three genotypes using MS supplemented with 1.0 mg/L N6-benzyladenine (BA) and 0.1 mg/L gibberellic acid (GA3). The maximum shoot number and shoot length for the three cultivars were recorded with 2.0 mg/L BA and 0.2 mg/L indole-3-butyric acid (IBA) in multiplication medium using nodal explants produced from microshoots. Nodal explants with one or two axillary buds cultured for three weeks initiated roots on medium supplemented with various concentrations of 1-naphthaleneacetic acid (NAA) or/and IBA in half-strength MS medium for adventitious rooting. The highest rooting response was with the combination of 0.2 mg/L NAA and 0.2 mg/L IBA. A combination of NAA and IBA resulted in a significant increase in the rooting ratio over NAA or IBA alone. In this medium, the root formation rate according to ranged from 68.9% for the BaeYun No. 3 genotype to 51.8% for the Hwanggeum genotype. We also investigated the influence of the concentration the polyamine phloroglucinol in rooting medium. For all three genotypes, the highest rooting ratio, longest root length, and greatest root number were observed in the treatments with 75-150 mg/L phloroglucinol. Most rooted plants were acclimatized successfully.

금호강 하류 소리쟁이군락의 무기물 및 중금속 축적의 변이 (Variation of Heavy Metal Accumulation and Inorganic Matter of Rumex crispus Community from Kumho Riverside)

  • 박태규;박용목;송승달
    • The Korean Journal of Ecology
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    • 제22권3호
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    • pp.139-144
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    • 1999
  • 오염된 환경에서 널리 군락을 이루는 소리쟁이의 생태적인 생존전략을 밝히기 위해 금호강 하류 지역인 침산교, 팔달교, 달서천 및 금호교 지역에서 생육하고 있는 소리쟁이의 무기물자 중금속의 축적양상을 분석하였다. 소리쟁이는 지하부에 비해 지상부의 빠른 생장으로 인해 T/R 비가 생육초기에 1.3∼3.7에서 후기에는 2,4∼4.6까지 차이를 보였고, 다른 식물에 비해 신속하게 군락을 형성하는 것으로 나타났는데, 오염이 심한 달서천에서 가장 높은 T/R비를 보였다. 식물체에 축적된 총질소와 인은 지하부에 비해 지상부에서 높게 유지되어 T/R비가 생육초기에는 각각 3.1∼6.1과 1.5∼4.5를 나타내었으나, 후기에는 각각 6.7∼17.3과 3.9∼8.3을 보였다. 흡수한 중금속류는 지하부에 비해 지상부에서 Cu와 Zn은 최고 3.6배와 1.7배, Fe와 Pb는 각각 1.5와 4.8배 높은 값이었고, 식물체당 축적량도 지상부에서 61∼85% 높은 분배율을 나타내었다. 식물체에 축적된 중금속은 Cu, Zn, Fe, Pb가 각각 89.7, 376.6, 2946.1 및 13.2 ㎍/g dw로 최고치를 보였고, Fe>Zn>Cu>Pb 순으로 지상부에서 높은 값으로 축적되었다. 소리쟁이는 엽육 비후와 함께 80%이상의 높은 함수량을 보였고, 지하부에 비해 지상부에서 빠른 생장을 보였다. 생육 초기부터 지상부에 보다 많은 질소와 인을 함유하며, 흡수한 Ca는 지하부에 축적해 두고 중금속은 지상부로 전이시킴으로써 오염된 중금속환경에서도 널리 군락을 이루는 것으로 사료된다.

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Seasonal Growth and Root Respiration of North American Ginseng

  • John, T.A. Proctor;Dean, Louttit;Jirong, Jiao
    • Journal of Ginseng Research
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    • 제22권3호
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    • pp.161-167
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    • 1998
  • American ginseng plants (Panax quinquefolium L.) of various ages were harvested every two weeks during each of three growing seasons and dry matter yield of components and root respiration determined. Shoot dry weight was about 0.5 g, 2.5 g and 4 g for 2, 3 and 4-year-old plants, respectively and fruit dry weight was as much as 50% of this in 3- and 4-year-.old plants. Root dry weight decrease by 30~50% as shoots emerged and at the end of the season was about 2 g, 3.5 g and 5 g in 2, 3, 4 and 5-year-old plants, respectively. Shoot and root dry weight were linearly related with an approximate 1:2 ratio. Root respiration rate at 2$0^{\circ}C$ in the dark was about 5 $\mu\textrm{g}$ CO2 g-1 DW(dry weight) min-1 in the early season, then doubled within 50 days as shoots emerged, and thereafter declined over the season to 2~5 $\mu\textrm{g}$ CO2 g-1 DW min-1. The Q10 for dark respiration over the interval from 10 to 2$0^{\circ}C$ was 1.58. Root respiration rate and shoot growth rate was positively linearly related in all ages of plants. Key words: Dry weight, partitioning.

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Chitosan 용액에 의한 목초 종자의 피복효과 (Coating Effects on Grass Seeds with Chitosan Solution)

  • 이주삼;조익환;안종호
    • 한국유기농업학회지
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    • 제6권1호
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    • pp.51-61
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    • 1997
  • This experiment was carried out to investigate the growth response of 3 grasses to seed coating with chitosan solution and the attempt was made to estimate adequate seed coating concentrations of chitosan solution in each grass for the growth to be stimulated. Three species used in this experiment were orchardgrass, tall fescue and reed canarygrass. Six different seed coating concentrations of chitosan solution were applied as 0%(control), 0.01%, 0.05%, 0.1% and 1.0%, respectively. the results were obtained as follows; 1. Dry weight of tiller(WT), leaf area(LA), dry weight of leaf(LW), dry weight of stem(SW), dry weight of shoot(SHW), biological yield(BY) and C/F ratio were significantly different between species. 2. Number of tillers per plant(NT), dry weight of tiller(WT), dry weight of leaf(LW), dry weight of root(RW), dry weight of shoot(SHW), biological yield(BY) and T/R ration were significantly different between seed coating concentrations of chitosan solution. 3. The adequate seed coating concentrations of chitosan solution for the growth stimulating effect were different between species. The highest values of yield components and dry weight of plant parts were obtained at 1% in orchardgrass and tall fescue, and 0.05% in reed canarygrass, respectively. 4. Growth stimulating effect of seed coating in each species were different. The highest values were obtained in leaf area(LA), dry weight of leaf(LW), dry weight of root(RW), dry weight of shoot(SHW) and dry weight of biological yield(BY) in orchardgrass. The values of dry weight of stem(SW) and C/F ration were highest in reed canarygrass. 5. An increase in number of tillers per plant(NT), dry weight of leaf(LW), dry weight of stem(SW) and dry weight of root(RW) according to seed coating was attributed to the increase in dry weight of shoot(SHW). Among the aboved increasing factors, the dry weight of leaf(LW) was a main factor for the increase in dry weight of shoot(SHW). 6. An increase in dry weight of leaf(LW), dry weight of stem(SW) and dry weight of root(RW) according to seed coating was attributed to the increase in biological yield(BY). Both the dry weight of leaf(LW) and dry weight of root(RW) were main factors for the increase in biological yield(BY).

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Perennial Ryegrass 품종의 계절적 생육특성 III. 가을철 생육의 품종간 차이 (Seasonal Growth Chrateristics of Perennial ryegrass varieties III. Varietal differences in autumn growth)

  • 김성규;이주삼;조익환
    • 한국초지조사료학회지
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    • 제12권1호
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    • pp.12-18
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    • 1992
  • 본 시험은 가을철 생육기에 perennial ryegrass 5개 품종을 공시하여 생육시기별 품종에 대한 생물학적 수량(지상부중+지하부중), 지상부중(엽중+경중), 근중, 엽면종, 지상부/지하부 비솔, 경수, 근장,근장/근중 및 1 경중을 조사하였으며 시험 결과는 다음과 같다. 1. 수량구성요소는 생육이 진행됨에 따라 악화되었다. 2. 가을철 수량구성요소의 비솔은 지상부 73%, 지하부 27%였다. 3. 생물학적 수량이 많았던 품종은 Tempo, Maprima 였으며 P-2, Caliente등이 적었다. 4. 수량구성요소는 Tempr 품종에서 경중, 근중, 근장/근중, 1 경중이 많았고 Caliente 품종은 엽중, 경중,엽면종, 지상부/지하부/ 비솔, 1 경중이 적었다. 5. 조사시기별 생물학적 수량은 4~5차(10월 4일 ~10월 8일 )에 가장 높았으며, 3~4차(9월 30일~10월 4일)에 가장 낮았다. 6. 품종별 생물학적수량은 Tempo는 5~6차(10월 8일 ~10월 12일), Marprima는 1~2차(9월 22일 ~9월 26일), Manhattan, P-2, Caliente는 4~5차(10월 4일 ~10월 8일)에서 많았다.

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유산균과 효모균 배양액 함유 액비 시용이 크리핑 벤트그래스의 생육에 미치는 영향 (Effect of Liquid Fertilizer Contained Medium of Lactobacillus sp. and Saccharomyces sp. on Growth of Creeping Bentgrass)

  • 김영선;함선규;이상진
    • 아시안잔디학회지
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    • 제24권2호
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    • pp.138-144
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    • 2010
  • 본연구는효모균(Saccharomyces sp.)과유산균(Lactobacillus sp.)을 포함하는 미생물배양액에 기능성성분이 포함하는 기능성비료의 시비가 크리핑벤트그래스의 잔디품질과 생육에 미치는 영향에 대해 조사하였다. 처리구는 비료의 종류에 따라 무처리구(NF), 대조구(CF), 미생물배양액(MO; CF+MO),철 함유 미생물배양액(MO-Fe; CF+MO-Fe), 그리고 황함유 미생물배양액(MO-S; CF+MO-S)였다. 시험 전후 토양분석결과, pH는 시험 후에 전보다 모든 처리구에서 약간 증가하였고, 시험 종료 후 처리구간의 토양화학성은 비슷하여 공시비료의 시비는 토양환경에 거의영향을 미치지않았다. 잔디생육 조사결과, 엽색지수와 엽록소지수는 무처리구와 비교할 때, 관행구와 처리구에서 높았고, 관행구와 처리구들 사이에는 조사시기별 변화경향은 비슷하나 MO처리구에서 약간 높게 나타났다. 잔디뿌리는 MO와 MO-Fe에서 더 길었다. 잔디의 지상부와 지하부 생육을 조사결과, 지상부에서는 MO와 MO-S가, 지하부에서는 MO와 MO-S가 관행구보다 높았으며, MO처리가 NF와 CF보다 각각 26%와 6%증가하여 MO처리가 잔디생육에 가장 효과적이었다. T/R ratio는 모두 무처리구보다 증가였으나 처리구별 차이는 나타나지 않았다. 잔디 지상부 지하부의 무기성분함량을 CF와 비교할 때, 지상부에서는 CF보다 MO, MO-Fe 및 MO-S 처리구에서 질소와 인 함량 증가하였고, 지하부에서는 인 함량이 증가하였다. 본 연구 결과, 유산균과 효모균의 처리는 크리핑벤트그래스의 뿌리생육과 양분흡수를 촉진하여 잔디품질과 성장을 향상시키는 잔디성장촉진 기능이 있는 비료임을 알 수 있었다.

무기적(無機的) 환경요인(環境要因)이 잣나무 유묘(幼苗)의 생육(生育)에 미치는 영향(影響)에 관(關)한 연구(研究)(III) -중량생장(重量生長)과 T/R율(率)에 대(對)한 피음처리(被陰處理) 및 단근(斷根)의 영향(影響) (Effect of Inorganic Environmental Factors on the Growth of Pinus koraiensis Seedlings III -The Influence of Shading Pretreatment and Root Cutting on the Weight Growth and T/R Ratio)

  • 김영채
    • 한국산림과학회지
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    • 제76권3호
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    • pp.218-229
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    • 1987
  • 본(本) 연구(硏究)는 광환경(光環境) 및 근장(根長)의 정도(程度)를 각기 달리한 잣나무 유묘(幼苗)의 중량(重量) 생장(生長)을 측정(測定) 고찰(考察)한 것으로 그 결과(結果)는 다음과 같았다. 1) 묘목(苗木)의 중량(重量) 생장(生長)은 상대광도(相對光度) 63%구(區)가 가장 높게 나타났으나 전체적(全體的)으로는 피음(被陰)의 정도(程度)가 강(強)할 수록 점차(漸次)로 감소(減少)하는 경향(傾向)을 보였으며, 단근(斷根) 처리(處理)의 경우는 1/4 단근구(斷根區)가 타(他) 처리구(處理區)에 비(比)해 제일 높았다. 2) 묘목(苗木)의 T/R율(率)값은 광도(光度)가 낮았을 때와 단근(斷根) 정도(程度)가 강(強)할 수록 각각(各各) 그 값이 점차(漸次)로 높게 나타나서 결국(結局) 피음(被陰) 및 단근(斷根)의 정도(程度)가 강(強)할 수록 묘목(苗木)의 생장(生長)은 약세(弱勢)를 나타냈다. 3) 그리고 광도(光度) 및 단근량(斷根量)과 T/R율(率) 간(間)에는 2차(次) 회귀관계(回歸關係)에 있음을 알 수 있었다.

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생장조절체 처리가 원통형 종이포트묘의 도장 억제 및 근권부 발달에 미치는 영향 (Effects of Different Application Approaches with Diniconazole on the Inhibition of Stem Elongation and the Stimulation of Root Development of Cylindrical Paper Pot Seedling)

  • 장동철;수찬;김시홍;김대훈;김재경;허재윤;넉탕부;최기영;김일섭
    • 생물환경조절학회지
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    • 제29권4호
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    • pp.365-372
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    • 2020
  • 본 연구는 원통형 종이포트 토마토 육묘시 Diniconazole의 처리방법이 도장억제 및 근권발달에 미치는 영향을 검토하기 위하여 수행되었다. 그 결과, 엽면적, LAR, 초장, 충실도, 생체중, RGR 및 R/S 에서 시험구간 유의한 차이를 보였다. 동일한 농도를 처리했을 경우, 근권부와 지상부의 흡수도 차이로 인해 저면관수가 엽면살포에 비해 도장억제에 효과적이었다. 저면관수는 엽면시비의 10분의 1의 농도만으로도, 20~30%정도의 동일한 도장억제 효과를 얻을 수 있었다. 디니코나졸 처리에의한 근권부 반응이 흥미로웠는데, 저면관수시 총근장, 근권부피, 평균 근경 및 근단수가 증가하였다. 특히, 0.3mm 이하의 초미세근이 감소하고 0.3~0.6mm의 세근이 증가하였다. 따라서 원통형 종이포트 육묘시 저면관수를 하는 것이 기존 엽면시비에 비해 사용량이 적으면서도 도장억제 및 근권부 활착률을 높힐 수 있을 것으로 판단된다.

지렁이분립의 혼합상토가 고추유묘의 생육에 미치는 영향 (Effect of Earthworm Cast Mixtures on the Growth of Pepper(Capsicum annuum L.) Seedlings)

  • 전하준;조익환
    • 한국유기농업학회지
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    • 제4권1호
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    • pp.75-84
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    • 1995
  • This study was carried out to find the effects of the mixtures of earthworm cast, peatmoss, and vermiculite as a vegetable plant growth medium on the growth pepper seedlings. The mixed ratios of earthworm cast-peatmoss-vermiculite were 40-20-40, 40-30-30, 40-40-40, 50-20-30, 50-30-20, 60-10-30, 60-20-20 and 60-30-10%. The results of the study are as follows: 1, There was a significant difference of plant length, leaf area, shoot dry weight, root dry weight and biological yield per plant for growth stages and mixed ratios(p<0.05). But there was no significant interactions for both of them. 2. The nursery soil with earthworm cast was generally higher than the control treatment in shoot dry weight, root dry weight adn biological yield per plant. The shoot dry weight and biological yield per plant were high in the treatment including 60% of the earthworm cast to the 3rd week and in the one including 50% from the 4th week. But in root dry weight, the treatment including 40% of it was higher than treatment of the others. 3. The shoot dry weight per plant in treatments consisting both of 40% of earthworm, 40% of peatmoss and 20% of vermiculite and of 60%, 10% and 30% was more significant than that in the control treatment(p<0.05). 4. The average relative growth rates of shoot dry weight, root dry weight and biological yield for all treatments were higher than the ratio of control treatment except the ration of the treatment consisting of 60% of earthworm, 20% of peatmoss and 20% of vermiculite.

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Physiological Response of Tetrapleura tetraptera (Schum. and Thonn.) Taub. to Soil Textural Class, Moisture and Light Intensity

  • Akinyele, Adejoke O.;Wakawa, Lucky Dartsa
    • Journal of Forest and Environmental Science
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    • 제33권2호
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    • pp.97-104
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    • 2017
  • Investigation was carried out on response of Tetrapleura tetraptera (Schum. and Thonn.) to soil, water and light with the view of its domestication and introduction to different ecological regions. The experiment was arranged in a factorial experiment of $3{\times}3{\times}3$ in a completely randomized design (CRD) with three replicates. The factors were: soil textural class (Loamy sand, Sand and Sandy clay loam), watering regime (daily, twice a week and once a week) and light intensity (100%, 75% and 50%). Soil textural classes had significant influence on collar diameter, stem height, number of leaflets, root/shoot ratio and relative growth rate of Tetrapleura seedlings. Seedlings grown on loamy sand recorded the highest mean value- 2.28 mm for collar diameter, stem height- 12.9 cm, number of leaflets- 19.9, chlorophyll b- $0.34mg\;mL^{-1}$, leaf relative water content- 27.4% and relative growth rate- $0.037mg\;g^{-1}\;day^{-1}$. Watering regime had significant influence on the collar diameter of Tetrapleura. Seedlings watered daily recorded the highest mean value- 2.25 mm for collar diameter. Light intensity significantly influenced collar diameter and root/shoot ratio. Seedlings exposed to 100% light intensity recorded higher mean value for collar diameter- 2.28 mm and root/shoot ratio- 1.481 cm. The interaction between soil textural class and light intensity significantly affected collar diameter, stem height and number of leaflets. Higher mean value for collar diameter (2.47 mm) stem height (13.25 cm) and number of leaflets (21.16) were recorded while the interaction between soil textural class, light intensity and watering regime was significant for only number of leaflets. Tetrapleura exhibited some level of tolerance to different soil texture, drought and light intensity. Therefore, Tetrapleura has the potentials to be raised in different ecological zones characterized by difference in soil, rainfall and amount of sunshine.