• Title/Summary/Keyword: root and leaves

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Effects of Gamma-ray Irradiation on Growth Characteristics and DNA Damage in Licorice (Glycyrrhiza uralensis) (감마선 조사가 감초(Glycyrrhiza uralensis)의 초기 생육 및 DNA 손상에 미치는 영향)

  • Ryu, Jaihyunk;Im, Seung Bin;Kim, Dong Sub;Ahn, Joon-Woo;Kim, Jin-Baek;Kim, Sang Hoon;Kang, Si-Yong
    • Journal of Radiation Industry
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    • v.8 no.2
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    • pp.89-95
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    • 2014
  • This study was conducted to determine the optimal dose of gamma-ray on the growth and nucleus DNA damage for mutation breeding in licorice. Gamma-rays irradiated to dry seeds with various doses (0 to 1000 Gy). Significant decreases in germination rate (%), survival rate (%) and growth characteristics (plant height, number of leaves, root length and fresh weight) were observed by dose of increased. $LD_{50}$ (lethal dose) was approximately 400 Gy to 500 Gy. Also, reduction doses ($RD_{50}$) of plant height, number of leaves, root length and flash weight were 428 Gy, 760 Gy, 363 Gy and 334 Gy, respectively. It is supplest that the optimal dose of gamma irradiation for licorice mutation induction might be about 400 Gy in this study. We also conducted comet assay to observe nucleus DNA damage due to gamma irradiation. In comet assay, a clear difference was identified over 300 Gy treatments. With increasing doses of gamma-ray in the range of 100 to 1000 Gy, the rate of head DNA was decreased significantly from 92.88% to 73.09%. Tail length(${\mu}m$) was increased as the dose of increased over 300 Gy. Growth characteristics (Germination rate, Survival rate, plant height, number of leaves, root length and fresh weight) were highly negatively ($P{\leq}0.01$) correlated with dose. While the tail length was highly positively ($P{\leq}0.01$) correlated with dose.

Resistance Induction by Salicylic Acid Formulation in Cassava Plant against Fusarium solani

  • Saengchan, Chanon;Phansak, Piyaporn;Thumanu, Kanjana;Siriwong, Supatcharee;Le Thanh, Toan;Sangpueak, Rungthip;Thepbandit, Wannaporn;Papathoti, Narendra Kumar;Buensanteai, Natthiya
    • The Plant Pathology Journal
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    • v.38 no.3
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    • pp.212-219
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    • 2022
  • Fusarium root rot caused by the soil-borne fungus Fusarium solani is one of the most important fungal diseases of cassava in Thailand, resulting in high yield losses of more than 80%. This study aimed to investigate if the exogenous application of salicylic acid formulations (Zacha) can induce resistance in cassava against Fusarium root rot and observe the biochemical changes in induced cassava leaf tissues through synchrotron radiation based on Fourier-transform infrared (SR-FTIR) microspectroscopy. We demonstrated that the application of Zacha11 prototype formulations could induce resistance against Fusarium root rot in cassava. The in vitro experimental results showed that Zacha11 prototype formulations inhibited the growth of F. solani at approximately 34.83%. Furthermore, a significant reduction in the disease severity of Fusarium root rot disease at 60 days after challenge inoculation was observed in cassava plants treated with Zacha11 at a concentration of 500 ppm (9.0%). Population densities of F. solani were determined at 7 days after inoculation. Treatment of the Zacha11 at a concentration of 500 ppm resulted in reduced populations compared with the distilled water control and differences among treatment means at each assay date. Moreover, the SR-FTIR spectral changes of Zacha11-treated epidermal tissues of leaves had higher integral areas of lipids, lignins, and pectins (1,770-1,700/cm), amide I (1,700-1,600/cm), amide II (1,600-1,500/cm), hemicellulose, lignin (1,300-1,200/cm), and cellulose (1,155/cm). Therefore, alteration in defensive carbohydrates, lipids, and proteins contributed to generate barriers against Fusarium invasion in cassava roots, leading to lower the root rot disease severity.

Effects of Amendments on Ginseng Root Rot Caused by Fusarium solani Population Changes of the Microorganisms in Soil (토양(土壤)개량(改良)이 Fusarium solani 에 의한 인삼근부병과(人蔘根腐病)과 미생물(微生物) 변동(變動)에 미치는 효과(效果))

  • Son, Suh-Gyu;Shin, Hyun-Sung;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.13 no.1
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    • pp.41-47
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    • 1985
  • Eighteen plant residues were added to soil and the amended soil was inoculated with chlamydospores (5,000 cells/g soil) of Fusarium solani causing root rot of ginseng in soil to test the effects of amendment using pea (Pisium sativa L.) as an index plant. Distributional conditions of microogranisms in soil were compared with each other before and after dealing with soil amendment by plant debries. Infection rate by index plant's infectivity showed a higher degree in the treatment of wheat crushed than in control group, and followed by stalk of sweet potato, chinese cabbage, ginseng leaves and soybean pod. On the other hand, the de­creasing order of infection rate was root of garlic, welsh onion, cabbage leaf and stalk, green onion stalk, wheat straw and barley straw. In comparison with control group, the propagules of fungi increased in the treatment of ginseng leaves, soybean ground, wheat crushed, maize stalk, and chinese cabbage, but decreased in the root of garlic, cabbage, and barley straw. Population of total bacteria increased in the treatment of soybean ground, chinese cabbage, radish stalk, welsh onion, and wheat crushed, but decreased in barley straw, tobaco root, ginseng stalk, and wheat straw. The numbers of actinomycetes increased only in the treatment of soybean ground in a comparison with control and also decreased in the garlic stalk and tobaco root. The propagules of Fusarium spp increased in the treatment of chinese cabbage, welsh onion, radish stalk, wheat crushed, and sweet potato stalk, wheat crushed, and sweet potato stalk, but decreased in the treat­ment of wheat straw, ginseng leaves, and cabbage than control.

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Residual Stress and Fracture Analysis of Thick Plate for Partial Penetration Multi-Pass Weldment

  • Kim, Seok;Shim, Yong-Lae;Song, Jung-Il
    • Journal of Mechanical Science and Technology
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    • v.16 no.9
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    • pp.1033-1039
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    • 2002
  • Partial penetration welding joint refers to the groove weld that applies to the one side welding which does not use steel backing and to both side welding without back gouging, that is, the partial penetration welding joint leaves an unwelded portion at the root of the welding area. In this study, we analyzed the residual stress and fracture on the thick metal plates that introduced the partial penetration welding method. According to the above-mentioned welding method, we could draw a conclusion that longitudinal stress and traverse stress occurred around the welding area are so minimal and do not affect any influence. We also performed the fracture behavior evaluation on the partial penetration multi pass welding with 25.4 mm thick plate by using the J-integral, which finally led us the conclusion that the partial penetration multi-pass welding method is more applicable and effective in handling the root face with less than 6.35 mm.

Molecular Characterization and Expression Analysis of Ascorbate Peroxidase in Codonopsis lanceolata (S. et Z.) Trautv

  • In Jun-Gyo;Lee Eun-Kyung;Kim Ha-Na;Yoon Jae-Ho;Lee Mee-Sook;Yang Deok-Chun
    • Plant Resources
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    • v.8 no.3
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    • pp.194-201
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    • 2005
  • A cytosolic ascorbate peroxidase, hydrogen peroxide-scavenging enzyme, was characterized from Codonopsis lanceolata. The cytosolic ascorbate peroxidase cDNA (CAPX) was 983 nucleotides long and possess an open reading frame of 753 bp with 251 amino acids (MW 27.9 kDa) with pI 5.61. The deduced amino acid sequence of CAPX shows high homology to other known cytosolic APXs ($78{\sim}83%$), but the CAPX was clustered independently from compared ten plant APXs. The CAPX gene was highly expressed in leaf and stem tissues, but not in root. When Codonopsis leaves cut using scalpel were soaked in 1 mM hydorgen peroxide, the expression of CAPX gene was suppressed.

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Effect of Microbial Fertilizers on the Yield of Aitari Radish (생균제 미생물비료가 알타리무의 수량에 미치는 영향)

  • 김경제;김석균
    • Korean Journal of Organic Agriculture
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    • v.6 no.2
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    • pp.107-116
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    • 1998
  • The result of application of the organic manure obtained from livestocks fed with BLCS in the altari radish under structure were as follow. The no. of leaves, leaf, root length, root width, and total weight of altari radish treated with dong of fowl(BLCS) were the highest in any other treatment, and cattle drop-ping (BLCS) was higher, also. The contents of total N, P2O5, K2O, CaO, MgO, and sugar content in the altari radish treated with dong of fowl(BLCS) were the highest, and the second was cattle dropping (BLCS). The contents of OM, P2O5, K, Ca, Mg, and CED of soil treated with microorganism were the highest in the cattle dropping (BLCS) in any other plots, however. In general, effect of cattle manure fed with BLCS on the altari radish yield and mi-crobial activity were higher than cattle manure did not feed BLCS.

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Effect of Applied Substrates on Foliage Growth in Hydro-Culture (수경재배 시 첨가매질이 관엽식물의 생장에 미치는 영향)

  • Jang, Hye-Sook;Lee, Sang-Gyu;Moon, Ji-Hye;Pak, Chun-Ho
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.460-467
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    • 2009
  • This research was conducted for the purpose of investigating the influence substrates addition such as germanium or Granite porphyry gravel have on the growth of foliage, on the inorganic compositions of leaves as well as on root activity, in order to discover effective means of introducing plants to the indoors. Syngonium podophyllum, Dracaena sanderiana, Epipremnum aureum and Hedera helix were used as study subjects to which soil (mixed top-soil: Sunshine Mix No. 2, USA), tap water, tap water with germanium (300g), tap water with Granite porphyry gravel (300g) were added respectively and formulated. Studies on growth variations according to substrates addition indicate that growth of Syngonium podophyllum was most sluggish under tap water only treatment. Plant growth was most active under geranium-gravel treatment. In the case of Dracaena sanderiana, treatment of substrates addition had no meaningful influence on plant growth. However, the growth of Epipremnum aureum and Hedera helix was also shown to be most active under geranium-gravel treatment. Geranium-gravel treatment was shown to be particularly beneficial for root length, number of roots and dry weight. Root activity was analyzed on ten day intervals and there was discernable difference in the root activity of all the plants according to the varying treatments. In the case of the Syngonium podophyllum, the root activity was sluggish in all three types of treatment on the 10th day but improved gradually. On the 30th to the 50th day of the experiment, the root activity was found to be best under the Granite porphyry gravel treatment. In the case of the Dracaena sanderiana, the root activity was most active under the geranium-gravel treatment from the 10th to the 40th day but deteriorated from the 50th day. However, there was no significant different in all three treatments from the 60th day onwards. Analysis of the inorganic components of the leaves indicates that, while Ca and Mg were higher in the geranium than in the Granite porphyry gravel, they do not directly influence the content of inorganic components in the leaves. The results indicate that Epipremnum aureum and Hedera helix grow better under hydro-culture than when grown in soil and growth under hydro-culture is shown to increase when germanium is added to tap water.

Effect of Chicory (Chicorium intybus L.) Root Diameter on Chicon Growth Characteristics (치커리 근경이 치콘 생육에 미치는 영향)

  • Seo, Hyun-Taek;Won, Jae-Hee;Jeon, Shin-Jae;Kang, Ho-Min
    • Journal of Bio-Environment Control
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    • v.20 no.2
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    • pp.139-143
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    • 2011
  • This study was conducted to investigate correlation between external factors of chicory root and chicon fresh weight, whereby, to provide basic information of external yardstick of producing standardized chicon. For correlation between external factors (root diameter, root weight) of chicory root and chicon fresh weight were highly correlated. The relationship between external factors (root diameter, root weight) of chicory root and chicon fresh weight, root diameter was strongly affecting chicon fresh weight due to significant at its regression coefficient ($6.06^{***}$). In order to verity this correlation, the root diameter (x) of chicory that were 3 different varieties were based on the 4 different root diameter treatments, such as x ${\leq}$ 30 mm, 30 < x ${\leq}$ 40 mm, 40 < x ${\leq}$ 50 mm and x > 50 mm. The chicon production increased as root diameter increased and the chicon fresh weight, chicon width, and the number of leaves increased as well. Therefore, the measure of root diameter of chicory instead of the internal factors (carbohydrate, and so on.) can be used to predict the chicon fresh weight.

Changes in Protein Contents and Activities of Proteolytic Enzymes in Medicago sativa During Regrowth

  • Kim, Tae-Hwan
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.357-363
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    • 1994
  • An expreiment with non-nodulating alfalfa (Medicago sativa L.) plants was designed to investigate the changes in protein contents and the activities of proteolytic enzymes during a regrowth period of 24 d. Shoot removal caused a depression of root growth and significantly reduced protein contents in roots. An initial decline of root proteins for the first 10 d was followed by a rapid recovery from d 11 to 24. The major increase of regrowing shoot weight occurred also from d 11. The activities of aminopeptidase and endoprotease slightly decreased in regrowing leaves, while protein contents remains stable after shoot removal. Roots exhibited source behaviour with a rapid increase of endoprotease activities for the first 10 d of regrowth; about a 370% increase over the initial level was observed. Increase in endoprotease activity in roots coincided with the time of protein remobilization after shoot removal, indicating the important role of endoproteases in protein degradation.

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An Information Structure Graph: A Structural Formalization of Information Semantics

  • Lee, Choon-Yeul
    • The Journal of Information Technology and Database
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    • v.7 no.1
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    • pp.55-65
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    • 2000
  • Information semantics is a well-known issue in areas of information systems researches. It describes what data mean, how they are created, where they can be applied to ; thus, it provides indispensable information for management of data. This article proposes to formalize information semantics by the processes that data are created or transformed. A scheme is proposed to describe an information production structure, which is called an information structure graph. An information structure graph is a directed graph, whose leaves are primary input data objects and whose root and internal nodes are output objects. Information semantics is derived from an information structure graph that has data as its root. For this, rules are proposed to manipulate and compare graphs. The structural relationships among information structure graphs are mapped into semantic relationships among data.

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