• 방사선산업학회지
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• 제8권2호
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• pp.89-95
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• 2014

This study was conducted to determine the optimal dose of gamma-ray on the growth and nucleus DNA damage for mutation breeding in licorice. Gamma-rays irradiated to dry seeds with various doses (0 to 1000 Gy). Significant decreases in germination rate (%), survival rate (%) and growth characteristics (plant height, number of leaves, root length and fresh weight) were observed by dose of increased. $LD_{50}$ (lethal dose) was approximately 400 Gy to 500 Gy. Also, reduction doses ($RD_{50}$) of plant height, number of leaves, root length and flash weight were 428 Gy, 760 Gy, 363 Gy and 334 Gy, respectively. It is supplest that the optimal dose of gamma irradiation for licorice mutation induction might be about 400 Gy in this study. We also conducted comet assay to observe nucleus DNA damage due to gamma irradiation. In comet assay, a clear difference was identified over 300 Gy treatments. With increasing doses of gamma-ray in the range of 100 to 1000 Gy, the rate of head DNA was decreased significantly from 92.88% to 73.09%. Tail length(${\mu}m$) was increased as the dose of increased over 300 Gy. Growth characteristics (Germination rate, Survival rate, plant height, number of leaves, root length and fresh weight) were highly negatively ($P{\leq}0.01$) correlated with dose. While the tail length was highly positively ($P{\leq}0.01$) correlated with dose.

• The Plant Pathology Journal
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• 제38권3호
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• pp.212-219
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• 2022

Fusarium root rot caused by the soil-borne fungus Fusarium solani is one of the most important fungal diseases of cassava in Thailand, resulting in high yield losses of more than 80%. This study aimed to investigate if the exogenous application of salicylic acid formulations (Zacha) can induce resistance in cassava against Fusarium root rot and observe the biochemical changes in induced cassava leaf tissues through synchrotron radiation based on Fourier-transform infrared (SR-FTIR) microspectroscopy. We demonstrated that the application of Zacha11 prototype formulations could induce resistance against Fusarium root rot in cassava. The in vitro experimental results showed that Zacha11 prototype formulations inhibited the growth of F. solani at approximately 34.83%. Furthermore, a significant reduction in the disease severity of Fusarium root rot disease at 60 days after challenge inoculation was observed in cassava plants treated with Zacha11 at a concentration of 500 ppm (9.0%). Population densities of F. solani were determined at 7 days after inoculation. Treatment of the Zacha11 at a concentration of 500 ppm resulted in reduced populations compared with the distilled water control and differences among treatment means at each assay date. Moreover, the SR-FTIR spectral changes of Zacha11-treated epidermal tissues of leaves had higher integral areas of lipids, lignins, and pectins (1,770-1,700/cm), amide I (1,700-1,600/cm), amide II (1,600-1,500/cm), hemicellulose, lignin (1,300-1,200/cm), and cellulose (1,155/cm). Therefore, alteration in defensive carbohydrates, lipids, and proteins contributed to generate barriers against Fusarium invasion in cassava roots, leading to lower the root rot disease severity.

• 한국균학회지
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• 제13권1호
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• pp.41-47
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• 1985

18종(種)의 식물질(植物質)을 토양(土壤)에 첨가(添加)하여 개량(改良)한 토양(土壤)에 인삼근부병원(人蔘根腐病原)에 하나인 Fusarium solani의 후막포수(厚膜胞數)$5{\times}10^3\;Cells/g\;soil)$를 접종(接種)하고 여기에 지표(指標)가 되는 식물(植物) 로 완두(Pisium sativa L.)를 심어 이병성(罹病性) 정도(程度)에 의하여 개량효과를(改良效果) 조사하고 또한 개량물질(改良物質)의 처리(處理) 전(前)과 후(後)의 토양내(土壤內)의 미생물분포(微生物分布)의 변동(變動)을 비교(比較)하였다. 식물질(植物質)로 개량(改良)한 토양처리구(土壤處理區)에서 분쇄밀처리구에서는 대조구(對照區) 이상으로 이병성( 罹病性)이 높았고, 다음이 고구마 줄기, 배추, 인삼잎 및 콩깍지의 순이였다. 반면 마늘 뿌리를 처리한 토양에서는 이병률(罹病率)이 가장 낮아 효과적(效果的) 인 개량물질(改良物質)이었고, 다음에 파, 양배추, 들깨대, 밀짚, 보리짚등에서도 이병률(罹病率)이 감소(減少)하였다. 총균수(總菌數)는 파, 콩, 분쇄밀, 옥수수대, 배추등에서 다수(多數)하였고, 반면 밀짚, 마늘뿌리, 양배추, 보리짚 등의 처리구에서는 감소하였다. 세균수(細菌數)는 콩, 양배추, 무우줄기, 파, 분쇄밀처리구에서는 증가(增加)되었으나 보리, 담배뿌리, 인삼대, 밀짚등에서는 감소(減少)하는 경향을 나타냈다. 방선균(放線菌)의 분포(分布)는 콩처리구에서만 증가(增加)하였으나 기타는 대조구(對照區) 보다 감소(減少)하고 담배뿌리와 마늘대에서는 그 수(數)가 감소(減少)되었다. Fusarium spp의 분포수(分布數)는 배추, 파, 무우줄기, 분쇄밀, 고구마줄기 등의 처리구에서는 증가(增加)하고 반대로 밀짚, 인삼잎, 양배추등의 처리구는 대조구(對照區) 보다 감소(減少)하였다.

• Journal of Mechanical Science and Technology
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• 제16권9호
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• pp.1033-1039
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• 2002

Partial penetration welding joint refers to the groove weld that applies to the one side welding which does not use steel backing and to both side welding without back gouging, that is, the partial penetration welding joint leaves an unwelded portion at the root of the welding area. In this study, we analyzed the residual stress and fracture on the thick metal plates that introduced the partial penetration welding method. According to the above-mentioned welding method, we could draw a conclusion that longitudinal stress and traverse stress occurred around the welding area are so minimal and do not affect any influence. We also performed the fracture behavior evaluation on the partial penetration multi pass welding with 25.4 mm thick plate by using the J-integral, which finally led us the conclusion that the partial penetration multi-pass welding method is more applicable and effective in handling the root face with less than 6.35 mm.

• Plant Resources
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• 제8권3호
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• pp.194-201
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• 2005

A cytosolic ascorbate peroxidase, hydrogen peroxide-scavenging enzyme, was characterized from Codonopsis lanceolata. The cytosolic ascorbate peroxidase cDNA (CAPX) was 983 nucleotides long and possess an open reading frame of 753 bp with 251 amino acids (MW 27.9 kDa) with pI 5.61. The deduced amino acid sequence of CAPX shows high homology to other known cytosolic APXs ($78{\sim}83%$), but the CAPX was clustered independently from compared ten plant APXs. The CAPX gene was highly expressed in leaf and stem tissues, but not in root. When Codonopsis leaves cut using scalpel were soaked in 1 mM hydorgen peroxide, the expression of CAPX gene was suppressed.

• 한국유기농업학회지
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• 제6권2호
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• pp.107-116
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• 1998

The result of application of the organic manure obtained from livestocks fed with BLCS in the altari radish under structure were as follow. The no. of leaves, leaf, root length, root width, and total weight of altari radish treated with dong of fowl(BLCS) were the highest in any other treatment, and cattle drop-ping (BLCS) was higher, also. The contents of total N, P2O5, K2O, CaO, MgO, and sugar content in the altari radish treated with dong of fowl(BLCS) were the highest, and the second was cattle dropping (BLCS). The contents of OM, P2O5, K, Ca, Mg, and CED of soil treated with microorganism were the highest in the cattle dropping (BLCS) in any other plots, however. In general, effect of cattle manure fed with BLCS on the altari radish yield and mi-crobial activity were higher than cattle manure did not feed BLCS.

• 생물환경조절학회지
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• 제18권4호
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• pp.460-467
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• 2009

본 연구는 실내에 식물을 보다 효율적으로 도입하고자 수경재배 시 첨가매질(게르마늄, 맥반석)이 관엽식물의 생장과 잎의 무기성분, 뿌리활력에 미치는 영향을 구명하기 위하여 수행되었다. 싱고니움(Syngonium podophyllum), 드라세나 산데리아나(Dracaena sanderiana), 스킨답서스(Epipremnum aureum) 및 아이비(Hedera helix)를 식물재료로 사용하였으며 토양(혼합된 상토: Sunshine Mix No. 2, USA), 수돗물, 수돗물에 게르마늄(300g), 수돗물에 맥반석(300g)을 첨가하여 정식하였다. 첨가매질에 따른 생육변화는 싱고니움의 경우 수돗물만 처리한 구에서 가장 저조하였고 게르마늄 첨가구에서 생육이 가장 좋았으며, 드라세나 산데리아나는 처리구에 따른 생장의 차이가 거의 없는 반면, 스킨답서스와 아이비의 생장도 게르마늄 첨가구에서 가장 좋았다. 특히 뿌리의 생장에서 커다란 차이를 보였는데 근장, 근수 및 근중이 게르마늄 첨가구에서 가장 좋았다. 뿌리활력은 10일 간격으로 분석하였는데 작물마다 처리구에 따라서 변화가 있었는데 싱고니움의 경우 10일째는 3처리구 다 뿌리활력이 낮아졌으나 차츰 좋아져 30일과 50일째에는 맥반석 첨가구에서 가장 좋았다. 드라세나의 경우 10일째부터 40일째까지 게르마늄 첨가구에서 가장 좋았으나 50일째부터 뿌리활력이 나빠졌다. 그러나 60일 후에는 처리구간 큰 차이가 없었다. 식물체 잎의 무기성분 분석 결과, 게르마늄의 Ca과 Mg 함량이 맥반석 보다 높았지만 잎의 무기성분 함량에는 영향을 미치지 않는 것으로 나타났다. 이상의 결과에서 스킨답서스와 아이비는 배지재배보다 수경재배에서 생장이 좋았고, 수경재배시 수돗물에 게르마늄을 첨가하였을 때 생육이 증가하는 것으로 나타났다.

• 생물환경조절학회지
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• 제20권2호
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• pp.139-143
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• 2011

본 연구는 치커리 뿌리의 외적요인과 치콘의 생체중간에 관계를 분석하고, 이중 높은 상관관계를 보인 요인을 치콘 규격 생산에 적합한 종근의 외적 판단기준으로 이용할 수 있는 자료를 만들고자 수행하였다. 종근의 외적요인인 근경(0.726)과 근중(0.508)의 상관관계를 보면 모두 생체중과 정(+) 상관관계를 보였다. 치콘 생체중에 영향을 주는 종근의 외적요인인 근경과 근중 중 상대적으로 높은 영향인자를 찾기 위하여 다중회귀 분석을 실시한 결과 치커리 근경의 회귀계수($6.06^{***}$)만이 유의하여 치콘 생체중에 영향을 미치는 것으로 나타났다. 3가지 품종의 치커리를 근경(x) 크기별로 x ${\leq}$ 30mm, 30 < x ${\leq}$ 40mm, 40 < x ${\leq}$ 50mm, x > 50mm로 분류하여 치콘을 생산한 결과 거의 모든 품종에서 근경이 증가할수록 치콘 생체중, 치콘 폭, 엽수 등이 증가하였다. 이상의 결과로 보아 치커리 종근의 탄수화물 함량 등 내부요인을 분석하지 않고, 상대적으로 간편한 외적요인인 치커리 근경을 수확 후 측정하여 치콘의 생체중을 미리 판단할 수 있을 것으로 사료되었다.

• Journal of Plant Biology
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• 제37권3호
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• pp.357-363
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• 1994

An expreiment with non-nodulating alfalfa (Medicago sativa L.) plants was designed to investigate the changes in protein contents and the activities of proteolytic enzymes during a regrowth period of 24 d. Shoot removal caused a depression of root growth and significantly reduced protein contents in roots. An initial decline of root proteins for the first 10 d was followed by a rapid recovery from d 11 to 24. The major increase of regrowing shoot weight occurred also from d 11. The activities of aminopeptidase and endoprotease slightly decreased in regrowing leaves, while protein contents remains stable after shoot removal. Roots exhibited source behaviour with a rapid increase of endoprotease activities for the first 10 d of regrowth; about a 370% increase over the initial level was observed. Increase in endoprotease activity in roots coincided with the time of protein remobilization after shoot removal, indicating the important role of endoproteases in protein degradation.

• 정보기술과데이타베이스저널
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• 제7권1호
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• pp.55-65
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• 2000

Information semantics is a well-known issue in areas of information systems researches. It describes what data mean, how they are created, where they can be applied to ; thus, it provides indispensable information for management of data. This article proposes to formalize information semantics by the processes that data are created or transformed. A scheme is proposed to describe an information production structure, which is called an information structure graph. An information structure graph is a directed graph, whose leaves are primary input data objects and whose root and internal nodes are output objects. Information semantics is derived from an information structure graph that has data as its root. For this, rules are proposed to manipulate and compare graphs. The structural relationships among information structure graphs are mapped into semantic relationships among data.