• 한국수산과학회지
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• 제33권6호
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• pp.585-590
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• 2000

A hemolysin producing strain was isolated from Kum rivet estuary located in west part of Korea. In the process of identification the isolated strain was similar to V. mimicus but did not show characteristics of known Vibrio species; therefore, the strain was designated as Vibrio sp. D9 ( V. kumkang) tentatively and further identification study was carried out by comparing its bacteriological characteristics, Morphologically Vibrio sp. D9 was a typical straight roe with a polar flagellium. Among known Vibrio species no identical strains were found when using automatic bacteria identification system ($MicioLog^(TM)$system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp. D9 showed 18 and 13 different responses as compared to V. mimicus and V. cholerae, respectively. Clear hemolysis zones were observed with the strain against human and sheep blood agar plate, Hemolytic toxicity was confirmed by strong vascular permeability and fatal toxicity against mouse was also observed. Thus the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. D9 were salinity of $0{\~}5.0{\%}$, pH of $6.4{\~}9.8$, temperature of $15{\~}41^{\circ}C$, respectively.

• 한국동물위생학회지
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• 제35권1호
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• pp.33-37
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• 2012

As people's interest about wildlife animal increases, more wildlife animal centers are developing. In South Korea, currently 120 mammal species live, and 22 of them are designated as endangered species. It is important to protect these natural treasures and endangered species, because they are being harmed by human industrial activity. For example, road expansion and poaching have led to the destruction of wildlife animal habitats, which is increasing year by year. Nevertheless, data regarding the systematic treatment of wild animals that are in distress is lacking, and it is a reality that analysis of such data is also lacking. The Kangwon Wildlife Rescue Center investigates these types of distress experienced by mammals. By analyzing the shape of the wild animals killed or injured, the center seeks an efficient and systematic way to manage such distress. The data collected from the Wildlife Animal Center from September 2007 to August 2010 includes 195 different cases, and the animals point of rescue. There are 16 species, including 87 water deer (Hydropotes inermis), 39 raccoons (Nyctereutes procyonoides), 20 roe deer (Capreolus pygargus bedfordi), 10 goral (Nemorhaedus goral), 7 siberian weasels (Mustela sibirica), 6 otters (Lutra lutra) and etc. Within the first year (September 2007 to August 2008), the number of mammals rescued was 46. In the second year (September 2008 to August 2009), 60 animals were rescued. in the third year (September 2009 to August 2010), the Wildlife Animal Center had 89 animals. We can see that these numbers are increasing, which shows that people are interested in the well-being of these animals because they are reporting animal injuries more and more. The mains form of distress caused by humans are from car accidents 67 (34.4%). Traps, and other hunting tools used by poachers are responsible for 25 (13.0%). Also, human industrial activity thus indirectly led to the starvation 25 (13.0%). and the orphaning of young animals 37 (19.0%).

• 한국의류학회지
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• 제14권2호
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• pp.85-97
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• 1990

This study investigates, through the Ka-rae-ban-cha-do(가예반차도) in the Ka-rae-do-gam­eui-gue (가예도감의궤), how the system of costume used in ceremony exchanged during the middle, late the Yi dynasty and in the period of introducing Wastern civilization. The procedure of the royal marriage is also studied here. The procedures of Ka-rae were divided into the six traditional etiguettes(륙예) : Nap-che (납채) , Nap-gyng(납징), Go-gi(고기), Chaik-bi(책비), Chin-young(친영), and Dong-roe(동뢰). The change of the general structure and characteristic of the costume used in the ceremony in Ka-rae-ban-cha-do are as follows: The costume in Ka-rae-ban-cha-do can be divided into the Guard costume (호위복식) and the Ceremonial arms costume (의장복식). The most formal attire of the Guard costume, Yangkwan-Chobok (양관-조복) and Samo -Danryeong(사모-단령) with Huygpai(흉배) can be seem only in the Ka-rae of late king's. Of the Ceremonial arms costume, the most outstanding was Hongkun- Hongeui (홍건-홍의), which showed up in almost all Ka-rae-ban-cha-do. Heukkun-Changos$\cdot$Deogrei (흑건-창옷$\cdot$더그레) were worn as well. In the style of costume emphasis convenience and practicality rather than on beauty, because they had to carry the arms used in ceremony with them nevertheless by using primary colors it was very colorful. This costume in Ka-rae-ban-cha-do has little difference, but great changes are shown in Sunjongsunjongbi Ka-rae-ban-cha-do (순종순종비가례반차도). Most costume of the past was replaced with western hat and suit, so the duplicate state of our costume and western costume came into existence during the period of introducing western civilization.

• 혜화의학회지
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• 제15권2호
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• pp.75-93
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• 2006

Chang San-roe re-explained the contents from the 30th question to the 47th question of "Nan Jing" which refered to the Chang-Pu and physiological theories as follows through the western theory which wss different from the tranditioonal chinese theory 1. "Nan Jing" refered to the circulation and the coming into being of Yeong-Wi. He denied that it was classified Yeong from Wi or Eum from Yang. 2. He denied that Sam-Cho was separated to three parts and the theory of "Sam-Cho is name only, not shape", using western medical theory. 3. He denied the view about "Left side is kidney, right side is Myung-Mun" because it was incorrect that kidney was separated to two parts which were Su and Hwa or Eum and Yang 4. Re-explaining the contents of "Nan Jing"- the portions of heart and lungs, the rising and falling of liver and lungs, the relationships of five viscera and sound color smell taste fluid, the relationships of five viscera and nine hole, the relationships of five viscera and O-Chu, the physiological difference of the old and the young owing to the deflection of Yeong-Wi Chi-Heol- he thought that viewing the human body using theory of Eum and Yang, Five elements(五行) was a far-fetched interpretation, so he denied the tranditional chinese theory which understood human body's organs, physiology, pathology. 5. He explained the contents of "Nan Jing"- the portions of the colon and the small intestines, the funtion of the six bowls, the corespondence of Chang-Pu, physical peculiarity of liver, the anatomical aspects which were the sizes, weights, volumes and shapes of the Chang-Pu and the alimentary canal, Chil-Chung-Mun, Pal-Hoe-Hyeolthrough western anatomy and physiology in detail.

• 플랜트 저널
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• 제11권1호
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• pp.30-38
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• 2015

본 연구는 최근 도입된 단기사채를 통해 한국수출입은행의 대외채무보증과 연계하여 자산유동화 구조에 접목한 자산담보부 단기사채의 금리를 추정하고 해외 투자개발형 민자발전사업의 수주 확대를 위한 프로젝트파이낸스의 한 방안으로 이용할 수 있는지에 대한 것이다. 회사채 신용등급 AA-부터 BBB+까지 건설사 자산담보부 단기사채와 비교한 결과, 자산 유동화 구조와 접목한 한국수출입은행의 대외채무보증부 자산담보부 단기사채는 금리 경쟁력이 있고, 프로젝트의 자기자본수익률이 개선됨을 확인할 수 있었다. 특히, 본 금융구조는 회사채 등급이 낮은 시공사에 적용할수록 더 높은 자기자본수익률 확보가 가능함을 알 수 있었다. 이를 통해, 경쟁력 있는 자산담보부 단기사채 금융조건은 수요 창출 및 수익성 확보는 물론 우리기업의 해외 투자개발형 민자발전사업 수주확대에 기여할 것으로 기대된다.

• 한국식품영양학회지
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• 제29권2호
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• pp.237-245
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• 2016

In this study, the physicochemical and sensory characteristics of low-salt Myungran jeotkal (Alaskan pollock roe) were evaluated after fermentation at $4^{\circ}C$ and $20^{\circ}C$ with or without the addition of deep sea water, salt from deep sea water, and vegetable-origin lactic acid bacteria (Lactobacillus fermentum JS, LBF). When fermented at $20^{\circ}C$, the addition of LBF to Myungran jeotkal resulted in a slow increase in lactic acid content, followed by an abrupt increase after five days of fermentation. However, when fermented at $4^{\circ}C$, the lactic acid content did not change significantly. Further, when Myungran jeotkal fermented at $4^{\circ}C$, the pH decreased as lactic acid production increased. The salinity of Myungran jeotkal fermented at $4^{\circ}C$ and $20^{\circ}C$ was 7% and was not affected by fermentation period. When fermented at $20^{\circ}C$, volatile basic nitrogen and amino nitrogen contents increased with increasing duration of fermentation. Further, volatile acid content decreased, however, the content of amino nitrogen increased after 11 days of fermentation with LBF and no salt effects were observed. When fermented at $20^{\circ}C$ for 13 days, preference (sensory evaluation) was the highest in all experimental groups after 9 days of fermentation, and then decreased as the fermentation period increased. The free amino acid content was highest (1,648.8 mg/100 g) in Myungran jeotkal when sun-dried salt and LBF were added, 2.3 times higher than in the control.

• 미생물학회지
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• 제28권1호
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• pp.19-26
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• 1990

To examine the effects of sequence variations near the transcriptional start site on the rate of formation of the open complexes at bacteriophage $\lambda P_{R}$ promoter, two mutant promoters were created by site-specific mutagenesis using synthetic oligonucleotides. Mutant I coatains changes at positions -3 and -4 from TT to CC, thus having a 6-bp long G/C stretch between -10 region and transciptional start site (+1). Mutant II has changes at positions -5 and -6 from GG to AA, thereby having a 9-bp long A/T stretch between positions -11 and -3. Selective filter binding assays were performed to measure the rate of formation of the open complexes between the wild-type or two mutant $P_{R}$ promoters on 664 bp fragments and E. coli RNA polymerase at two temperatures. At 37.deg.C, the wild-type and two mutants showed similar rates for the formation of open complex. The second order rate constant $k_{a}$ and $\tau _{int}$, as determined from the .tau.-plot analysis, were $(6.0\pm0.4)\times10^{6}M^{-1}sec^{-1}$ and $11\pm5$sec, respectively. At 18.deg.C, however, the wild-type and two mutant promoters showed differences in the kinetic parameters. k for the wild-type promoter was (2.2$\pm$0.1)\times 10^{6}M^{-1}sec^{-1}$ and $\tau _{int}$ was 76$\pm$sec. Mutant I and II exhibited differences mainly in the rate of isomerization ($\tau_{int,I}=91\pm$10 sec, int,II=34$\pm$ sec), whereas the second order rate constant $k_{a}$ was similar to the wild type value. This result implies that at $18^{\circ}C$, the isomerization rate is determined by both protein conformational change and DNA melting, which are separable kinetically according to the 3-step mechanism of Roe et al.(1984,1985), and that the base changes affected mainly the rate of DNA melting as predicted.lting as predicted.

• 한국한의학연구원논문집
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• 제7권1호
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• pp.105-114
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• 2001

This study was carried out to investigate correlation with food preference of subjects in three constitution groups on the view point of constitutional medicine. The constitutions of 124 college students(male 80, female 44) was classified by QSCC II method. Food preference of the subjects was surveyed in accordance with suggested foods for each constitution and was analyzed using Hedonic scale. The distribution of the constitutional revealed that Soeum individuals accounts 53%, followed by ; Soyang 19%, Taeum 28%. For men, sorghum, potatoes, beef, scombroid, oyster, saury, octopus, codfish, walleye pollack, shrimp, croaker, pineapple, eggplant, leek, pumpkin, toenjang, draft beer, diluted liquor, coffee, cocoa were statistically different in food preference score by sasang constitution(p<0.05). For woman, chicken, pollack roe, a walleye pollack, persimmon, jujube, melon, citron, celery, burdock, vinegar, bean-paste soup, draft beer, ginseng, honey were statistically different in food preference score by sasang constitution(p<0.05). Association of sasang constitutions and food thought to be harmful or healthy to each constitution was analyzed. But Food preference score of food thought to be harmful or healthy were not statistically different in score among sasang constitutions. This results suggested that food intake patterns of subjects were associated with sasang constitutional food in parts.

• 경영과정보연구
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• 제35권4호
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• pp.21-36
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• 2016

본 연구는 2009년을 전후하여 전국의 지역농협을 대상으로 당기순이익 변화분 등을 중심으로 재무성과 분석을 실시하였다. 지역농협 최고경영자인 조합장선거결과를 활용하여 2009년도를 전후선거결과와 재무성과의 변화에 대한 분석을 시도하였다. 선거실시로 인한 조합장 교체가 강압적 교체의 한 유형으로 가정할 때, 선거 이전의 경영성과 악화가 조합장 교체로 이어졌을 유의한 가능성이 있음을 프라빗 분석으로 통해 확인하였고, 상임이사 제도를 운영 중인 조합의 조합장 교체가 있을 경우 (+)의 재무개선효과가 있음을 확인하였다. 또한 입지유형에 따른 농촌형과 도시형 조합으로 분류하였을 때 2009년을 전후하여 농촌형 조합의 당기순이익의 유의한 개선이 나타나는 것을 확인하였다. 시간이 다소 지난 2009년 전후의 지역농협 재무분석 데이터를 활용하면서 ROE, ROA등 일반적 경영성과 분석 자료가 아닌 당기순이익의 증감을 중심으로 분석하였음에도 불구하고, 국내 농협의 전국단위 분석으로 2015년 전국조합장 동시선거 결과와의 연계분석의 토대를 마련하였다는데 의의가 있다고 본다.

• Journal of Microbiology
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• 제33권4호
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• pp.339-343
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• 1995

We isolated a 10 kb Bam HI fragment originated from the chromosome of a $H_2O$$^2$-resistant mutant strain of Streptomyces coelicolor, which confer $H_2O$$^2$-resistance to S. lividance upon transformation. Among various subclones ot 10kb Bam HI fragment tested for their $H_2O$$^2$-resistant phenotype in S. lividans, a subclone containing 5.2 kb Bam HI-BglII fragment was found to be responsible for $H_2O$$^2$-resistance. The plasmid containing this 5.2 kb fragment was then transformed into S. coellicolor A3(2) at early and tested for their phenotype of $H_2O$$^2$-resistance and the change in various enzymes whose activity can be stained in the gel. We found out that the 5.2 kb insert DNA conferred $H_2O$$^2$-resisstance in S. coelicolor A3(2) at early phase of cell growth. The presence of this DNA also resulted in higher level of peroxidase compared with the wild type cell containing parental vector (pIJ702) only. Esterase activity was also higher in this clone. However, alcohol dehydrogenase activity decreased compared with the wild type. These results suggest that the presence of a gene in 5.2 kb BamHI-BglII DNA fragment causes multiple changes in S. coelicolor related to its response against hydrogen peroxide. The result also implies that not only peroxidase but also esterase may function in the defencse meahsnism agianst $H_2O$$^2$-.