• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.393-398
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• 2013

Lodging in the direct seeded rice cultivation on flooded paddy is being severe problem due to the lower production and grain quality at ripening stage. This study was conducted to examine the effect of Iprobenfos-metconazole (IPM) treated 50, 40 and 30 days before heading respectively as a plant regulator to reduce the lodging at ripening stage of direct seeding rice cultivation. The culm length treated with IPM, especially the 4th culm internode, was shortened more than with untreated plot, and the most effective time was at 30 days before heading. At 20 days after heading the flag leaves colour showed more greening than in the untreated leaves and the plot with IPM treatment was to be maintained longer compared to the control plot. The Nitrogen concentration of leaves with IPM was lower than in the control plot, and $SiO_2$ contents were higher than in the untreated plot and $SiO_2/N$ ratio was increased more in the IPM plot than in the control plot. The breaking strength of 3rd and 4th culm internode with IPM treatment was higher than in the untreated plot, and the lodging index was reduced in the IPM plot significantly and the field lodging also was reduced. As the results the rice production in the IPM plot was increased more due to be higher ripening ratio and seed grain weight compared to the untreated plot to be occurred the field lodging.

• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.159-167
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• 2014

In eukaryotes, proteins that are secreted into ER are post-translationally modified by N-glycosylation, the patterns of which are significantly different between plant and animal cells. Biotechnology industry has already produced a number of therapeutic glycoproteins in plant cells. However, the aberrant glycosylation of therapeutic recombinant proteins in plant systems can cause immune problems in humans. Therefore, it is important to develop strategies for producing non-glycosylated forms to preserve biological activity and native conformation by a peptide: N-glycanase (PNGase). In this study, we try to isolate PNGase T gene from tomato, which can use as a platform plant for biotechnology industry. We isolated a cDNA (GenBank Accession number KM401550) from tomato leaves with 1,767 bp, which encoded a polypeptide of 588 amino acids with a predicted molecular mass of 65.8 kDa. We also investigated the expression patterns of PNGase T during fruit ripening of tomato. The transcripts of PNGase T, which were constitutively induced in tomato fruit from green stage, were significantly increased and reached a peak at orange stage. After which, those transcripts were continuously reduced. The expression pattern of PNGase T was coincided well with transcripts profiles of metacaspase gene, LeMCA, and senescence-related gene members of ACC synthase, LeACS2, LeACS4, and LeACS6, for ethylene biosynthesis during fruit ripening. These results suggest that PNGase T is involved in a de-glycosylation process associated with senescence and fruit ripening.

• Food Science and Preservation
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• v.20 no.4
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• pp.475-481
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• 2013

This study was performed in order to compare the changes in fruit quality and antioxidant activity depending on the ripeness degree of the banana flesh and peel (unripe, ripe, and over ripe) when stored at room temperature for 6 days. The moisture contents showed differences such as the significantly lower maturity in the over ripe fruit peel and the moisture content increase in the fruit flesh during ripening. During the maturity stage, the L and b values decreased, while the a value increased. During ripening, the titratable acidity (TA) decreased, while the soluble solids contents (SS), sugar acid ratio (SS/TA), total sugar, and reducing sugar contents increased, respectively. Also during ripening, the glucose and the fructose contents increased considerably, thus resulting in the decrease of the sucrose content. In addition, this study revealed that the phenolic substance, which was part of the fruit skin, showed more favorable reaction to radical ability than the fruit flesh. Furthermore, the DPPH, ABTS radical scavenging ability, and reducing power showed better reaction for the fruit skin than for the fruit flesh, and there was a significant increase in the antioxidative activity as a result of the higher levels of phenolic substance. Therefore, maturity played an important role in changing the chemical composition and physiological activity of a banana. The unripe peels could be used as antioxidant ingredients and they could also enhance the biological activity in the utilization of by-products.

• Korean Journal of Microbiology
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• v.7 no.1
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• pp.10-21
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• 1969

The effect of glucose and 2-thiobarbituric acid on the biosynthesis of cell constituents such as protein, carbohydrate, DNA, RNA, phospholipid and PCA-soluble phosphate compounds in Chlorella duing the life cycle was measured, and the changes in the content of these main cellular components of the algal cell were analyzed in connection with the nuclear and cytoplasmic divison. In the normal autotrophic synchronous culture the contents of protein, RNA, and DNA in the cell showed a chracteristic changes according to the progress of cell development, increasing more or less throughout all the life cycle. The synthesis of protein is more prominent in the division period nad that of DNA is more active in the ripening period, while the synthesis of RNA is more rapid in the growing and ripening periods than other developmental stages. The period of division cycle was little affected by glucose in the medium, although the synchrony of the growth and cellular division was disturbed and the n value increased. The cotents of protein, carbohydrate, RNA nad DNA of the cell were increased by the glucose treatment throughout all the life cycle. On the other hand, both of cellular growth and division were retarded severely and the n value was decreased by the 2-thiobarbituric acid treatment throughout all the life cycle. On the other hand, both of cellular growth and division were retarded severely and the n value was decreased by the 2-thiobarbituric acid treatment. The synthesis of protein, carbohydrate, DNA, RNA and phospholipid of the cell was also retarded by 2-thiobarbituric acid. In the autotrophic, mixotrophic and 2-thiobarbituric acid-treated cultures, each having different mode cytoplasmic division, a common general schema occurring in the cell during the life cycle may be drawn as follows. The ratio of RNA to protein attains maximum value in the $L_1$-cell stage prior to the nuclear division and thereafter decreases during the periods of ripening and division. The ratio of PCA-soluble phosphate compounds to protein increased from the begining of the culture to $L_4$-cell stage successively and thereafter decreased gradually during the division period, while the ratio of protein to DNA kept almost constant up to the division period and thereafter increased during the division period. Therefore, it is presumed that the increase in the ratio of RNA to protein is to be an inducer of nuclear division and that the cytoplasmic division is induced by the increase in the ratio of protein to DNA.

• Journal of Bio-Environment Control
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• v.19 no.2
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• pp.93-96
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• 2010

Breaker stage tomato fruits that were stored at low temperatures show typical chilling symptoms such as uneven ripening and a high respiration. Experiments were performed to assess and compare these chilling injury symptoms of breaker stage tomato fruits, and to gather basic data that can be used to decide whether horticultural crops receive chilling injuries. Tomato fruits that had been sorted in the breaker stage were stored at $2^{\circ}C$ for 0, 3, 6, and 9 days, and then their respiration rates were measured at 3, 6, 9, 12, 24, 36, and 48 h after moving them to room temperature. This treatment was repeated twice on the same procedures, except the storage periods, which were changed to 0, 1, 2, 3, and 10 days. The respiration rate was increased in a 1 day storage treatment, and the increasing rate rose higher with extended storage periods. The $a^*$ value, which represents the surface color of tomato fruits, was measured 10 days after moving to them into room temperature. Those with an increased $a^*$ value rate got dull and showed uneven coloring after 2 days' storage treatment. These two factors, the respiration rate and $a^*$ value of the surface, showed a high correlation (r = 0.9716, p < 0.001). Therefore, the chilling injury of breaker stage tomato fruits can be diagnosed by measuring the respiration rate after moving them into room temperature, and the degree of chilling injury can also be assessed in terms of the respiration increase rate.

• Food Science and Biotechnology
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• v.15 no.2
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• pp.196-201
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• 2006

Freeze concentration of milk was carried out thorough the controlled recrystallization of ice in a multi-stage freeze concentrator. Artificial temperature control was used to induce ice recrystallization via a heat and cold shock process. In each stage of freeze concentration, the recrystallization time was fixed at 1, 2, 4, and 8 hr to compare the solute concentrate, yield, Brix, ice crystal size, and freezing point at each experimental condition. Higher concentrations of milk solids were seen with increased durations of recrystallization time, and a maximum total solids in the final product of 32.7% was obtained with a ripening time of 8 hr in a second stage process. Milk solid yield decreased according to the solute concentration and recrystallization time. The results of Brix and ice crystal size showed a positive correlation with recrystallizaiton time. These results suggest the possibility of freeze concentration being of practical use in the dairy industry.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.3
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• pp.219-225
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• 1981

Strong wind of 6.5m/s from electric fan was applied to rice at heading stage to find out the nature of wind damage to panicle. Damaged panicles caused by typhoon were sampled out of field to measure the yield and milling components. Wind-treated glumes were developed into two forms, fertile and sterile, and each of them showed all degree of glume discoloration. The number of fertile grains were higher than sterile grains in a damaged panicle. Averagesdegree of glume discoloration was higher in not flowered glume than in flowered glume. The more recently flowered glumes resulted the higher degree of discoloration among flowered glume. The percent of sterile grain to total number of grains was the highest at stage of just flowering. The glume discoloration representing wind damage at heading state advanced in degree in ten days after treatment compared to that just after treatment. Grain length and grain width were decreased with the increased degree of glume discoloration, while grain thickness was not changed greatly. Chalkiness was a little higher in damaged grain than in normal grain without definite trends by degree of discoloration. Ripening ratio, 1000 grain weight and yield decreased with increased degree of glume discoloration in damaged panicle by typhoon. Decreasing order in yield was white rice, rough rice and brown rice. Ratio of brown rice to rough rice increased, white rice to brown rice decreased, and milling recovery was not changed greatly with increased degree of 히ume discoloration.

• Journal of Korea Foundry Society
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• v.15 no.5
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• pp.483-493
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• 1995

The microstructural changes during semi-solid state processing of hypereutectic Al-Si alloy has been investigated in the present study. Stirring of semi-solid slurry results in the morphological changes of the primary Si particles, i.e. from angular rod shape to near-spherical shape. Besides the spherodization of primary Si particles, the average particle size increases, especially, at much higher rate in the final stage than that in the early stage of stirring. Various microstructure characterization techniques, such as anisotropic etching, SEM imaging and ECP analysis, reveal that the spherodization of primary Si particles occurs by the combinations of the mechanisms of coalescence, fracture, and wear of the individual particles. Isothermal shearing of hypereutectic Al-Si at $580^{\circ}C$ shows that spherical ${\alpha}-Al$ particles are formed by the dissociation of Al-Si eutectic structure at the early stage of isothermal shearing. The spherical ${\alpha}-Al$ particles gradually grow by the mechanisms of Ostwald ripening and coalescence of the particles.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.326-332
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• 2008

This study was conducted to evaluate an early-maturing rice cultivars adaptable to early transplanting cultivation in Yeongnam plain area. High temperature during the ripening stage of paddy rice under the early-transplanting cultivation in Yeongnam plain was to influence in several agronomic traits such as number of spikelet, ripening ratio, grain appearance of milled rice, and yield. 'Gounbyeo' and 'Junghwabyeo' were better than the others in grain appearance of milled rice and ripening ratio, but their yield potential were lower than that of the other varieties. Moreover, 'Gounbyeo' and 'Junghwabyeo' showed high ratio of broken rice. 'Unkwangbyeo' was the highest in yield and eating quality among the tested varieties, but also high percentage of white core and belly in milled rice. Therefore, in order to recommend those cultivars to farmers, it should be needed to establish an optimal cultivation method, such as transplanting density, fertilizer application, and harvesting time, etc. consequently, it should also be required to develop a new varieties with high ripening capability under high temperature condition.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.3
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• pp.241-247
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• 2001

Boundary line method was adopted to analyze the relationships between rice yield and meteorological conditions during rice growing period. Boundary lines of yield responses to mean temperature($T_a$) and sunshine hour( $S_{h}$) and diurnal temperature range($T_r$) were well-fitted to hyperbolic functions of f($T_a$) =$$\beta$_{0t}$(1-EXP(-$$\beta$_{1t}$ $\times$ ($T_a$) ) and f( $S_{h}$)=$$\beta$_{0t}$((1-EXP($$\beta$_{1t}$$\times$ $S_{h}$)), to quadratic function of f($T_r$) =$\beta$$_{0r}$(1-($T_r$ 1r)$^2$), respectively. to take into account to, the sterility caused by low temperature during reproductive stage, cooling degree days [$T_c$ =$\Sigma$(20-$T_a$] for 30 days before heading were calculated. Boundary lines of yield responses to $T_c$ were fitted well to exponential function of f($T_c$) )=$\beta$$_{0c}$exp(-$$\beta$_{1c}$$\times$$T_c$ ). Excluding the constants of $\beta$$_{0s}$ from the boundary line functions, formed are the relative function values in the range of 0 to 1. And these were used as yield indices of the meteorological elements which indicate the degree of influence on rice yield. Assuming that the meteorological elements act multiplicatively and independently from each other, meteorological yield index (MIY) was calculated by the geometric mean of indices for each meteorological elements. MIY in each growth period showed good linear relationship with rice yield. The MIY's during 31 to 45 days after transplanting(DAT) in vegetative stage, during 30 to 16 days before heading (DBH) in reproductive stage and during 20 days after heading (DAH) in ripening stage showed greater explainablity for yield variation in each growth stage. MIY for the whole growth period was calculated by the following three methods of geometric mean of the indices for vegetative stage (MIVG), reproductive stage (HIRG) and ripening stage (HIRS). MI $Y_{I}$ was calculated by the geometric mean of meteorological indices showing the highest determination coefficient n each growth stage of rice. That is, (equation omitted) was calculated by the geometric mean of all the MIY's for all the growth periods devided into 15 to 20 days intervals from transplanting to 40 DAH. MI $Y_{III}$ was calculated by the geometric mean of MIY's for 45 days of vegetative stage (MIV $G_{0-45}$ ), 30 days of reproductive stage (MIR $G_{30-0}$) and 40 days of ripening stage (MIR $S_{0-40}$). MI $Y_{I}$, MI $Y_{II}$ and MI $Y_{III}$ showed good linear relationships with grain yield, the coefficients of determination being 0.651, 0.670 and 0.613, respectively.and 0.613, respectively.