• Journal of Animal Science and Technology
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• v.51 no.3
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• pp.241-248
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• 2009

Metabolic trial with 3 fistulated sheep was conducted in a 3 $\times$ 3 Latin square design and feeding trial with 24 Hanwoo steers in 12 month of age for 20 months was conducted to investigate the replacing effect of rice straw with fermented spent mushroom (Flammuliua velutipes) compost (FSMC) on fermentation characteristics, ruminal effective degradabilty and whole tract digestibility of nutrients in sheep, and to examine the growth performance of Hanwoo steers. Experimental diets for the metabolic trial with sheep were commercial concentrates and rice straw in the ratio of 70 : 30 (CON, DM basis). Same concentrate with 30% FSMC and 70% rice straw (FSMC-30) and 60% FSMC and 40% rice straw(FSMC-60). Diets for Hanwoo steers in three treatments were same as for metabolic trial in replacing ratio of rice straw with FSMC. pH of rumen fluid in sheep was not affected by FSMC. Ammonia-N content in the rumen fluid was highest in the sheep fed FSMC-60 at 3h (P<0.045). The CON diet increased (P<0.001) acetate proportion at 1h and 3h post feeding compared to FSMC-60 diet while propionate proportion was highest in the sheep fed FSMC-60 diet for all the sampling times (P<0.027~P<0.002). Increased proportion of butyrate was observed at 30 min prior to feeding (P<0.031), and 1h (P<0.011) and 6h(P<0.039) post feeding from sheep fed FSMC-30 diet compared to those from sheep fed other diets. Effective degradability in the rumen was not influenced by experimental diets. Whole tract digestibility of crude protein (P<0.031) and neutral detergent fiber (P<0.006) tended to be increased in the sheep fed CON diet while corresponding values were lowest in the sheep fed FSMC-60 diet. Total body weight gain of Hanwoo steers for 8 months was not different among diets, thus daily body gain was not influenced by the experimental diets.

• Applied Biological Chemistry
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• v.33 no.2
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• pp.133-137
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• 1990

The effects of soil environmental conditions on the rate of degradation of fungicide IBP (Iprobenfos, S-benzyl O, O-diisopropyl phosphorothioate) in the soils under flooded condintions were examined in the laboratory. IBP in soil was degraded more slowly under flooded conditions than under upland conditions. The degradation greatly varied among soils, and the degradation rate was negatively correlated with the content of soil organic matter. Degradation of IBP was influenced by the soil temperature and the amount of IBP applied. The rate of degradation in soil was remarkably inhibited by the amendment of rice straw but not affected by the treatment of mixed-fertilizer, and insecticide fenitrothion and herbicide butachlor. The degradation of IBP was assumed to be due to microorganisms, especially aerobic microbes, as no degradation was observed in sterilized soil.

• Journal of Korean Society on Water Environment
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• v.32 no.1
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• pp.108-114
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• 2016

Sediment-laden water leads to water quality degradation in streams; therefore, best management practices must be implemented in the source area to control nonpoint source pollution. Field monitoring was implemented to measure precipitation, direct runoff, and sediment concentrations at a control plot and straw-applied plot to examine the effect on sediment reduction in this study. A hydrology model, which employs Curve Number (CN) to estimate direct runoff and the Universal Soil Loss Equation to estimate soil loss, was selected. Twenty-five storm events from October 2010 to July 2012 were observed at the control plot, and 14 storm events from April 2011 to July 2011 at the straw-applied plot. CN was calibrated for direct runoff, and the Nash-Sutcliffe efficiency and coefficient of determination were 0.66 and 0.68 at the control plot. Direct runoff at the straw-applied plot was calibrated using the percentage direct runoff reduction. The estimated reduction in sediment load by direct runoff reduction calibration alone was acceptable. Therefore, direct runoff-sediment load behaviors in a hydrology model should be considered to estimate sediment load and the reduction thereof.

• Journal of Microbiology and Biotechnology
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• v.28 no.2
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• pp.305-313
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• 2018

A microbial consortium, TMC7, was enriched for the degradation of natural lignocellulosic materials under high temperature. TMC7 degraded 79.7% of rice straw during 15 days of incubation at $65^{\circ}C$. Extracellular xylanase was effectively secreted and hemicellulose was mainly degraded in the early stage (first 3 days), whereas primary decomposition of cellulose was observed as of day 3. The optimal temperature and initial pH for extracellular xylanase activity and lignocellulose degradation were $65^{\circ}C$ and between 7.0 and 9.0, respectively. Extracellular xylanase activity was maintained above 80% and 85% over a wide range of temperature ($50-75^{\circ}C$) and pH values (6.0-11.0), respectively. Clostridium likely had the largest contribution to lignocellulose conversion in TMC7 initially, and Geobacillus, Aeribacillus, and Thermoanaerobacterium might have also been involved in the later phase. These results demonstrate the potential practical application of TMC7 for lignocellulosic biomass utilization in the biotechnological industry under hot and alkaline conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.5
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• pp.530-537
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• 1998

The proportion of dry matter (DM) and mineral elements (Ca, Mg, P, Na, K, Zn) released from eight feeds (2 rice straws, RSI and RS2; 2 grasses, NB21 and guinea; 2 leguminous fodders, glyricidia and erythrina; jak leaves and rice bran) were studied using the nylon bag procedure. Bag incubations up to 10 days were performed in the rumen of cows fed on a ration consisting of 50% wheat straw and 50% hay. Both the type of feed and the incubation time in the rumen significantly influenced (p < 0.01) the proportion of minerals released. In legumes, jak leaves and rice bran about 80% of the potentially degradable DM fraction was solubilized within 24 h in the rumen, and with the grasses, rice straws and jak leaves a considerable proportion of DM was released between 48 and 240 h in the rumen. During the early hours of incubation (up to 24 h) there were distinct differences between and within the feed classes in their ability to release all mineral elements studied. In all test feeds, high proportions of Mg and K were released within 24 h. Some feeds showed a tendency to ad/absorb Ca (grasses, rice straws and rice bran), P (jak leaves, rice straws), Na (glyricidia and rice bran) and Zn (jak leaves) from water and rumen fluid, and this was partly related to the low initial concentration. In terms of absolute quantity of mineral released, legumes (erythrina is superior to glyricidia) are a good source of Ca, Mg, P and Zn, and jak leaves a good source of Ca and Na. Within grasses, guinea contains appreciable quantity of available Mg and P. Rice bran is rich in available Mg, P and Zn.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.356-364
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• 2009

The current ruminant feeding models require parameterization of the digestion kinetics of carbohydrate fractions in feed ingredients to estimate the supply of nutrients from a ration. Using an automated gas production technique, statistically welldefined digestion rate of carbohydrate, including soluble carbohydrate, can be estimated in a relatively easy way. In this study, the gas production during in vitro fermentation was measured and recorded by an automated gas production system to investigate degradation kinetics of carbohydrate fractions of a wide range of ruminant feeds: corn silage, rice straw, corn, soybean hull, soybean meal, and cell mass from lysine production (CMLP). The gas production from un-fractionated, ethanol insoluble residue and neutral detergent insoluble residue of the feed samples were obtained. The gas profiles of carbohydrate fractions on the basis of the carbohydrate scheme of the Cornell Net Carbohydrate and Protein System (A, B1, B2, B3 and C) were generated using a subtraction approach. After the gas profiles were plotted with time, a curve was fitted with a single-pool exponential equation with a discrete lag to obtain kinetic parameters that can be used as inputs for modern nutritional models. The fractional degradation rate constants (Kd) of corn silage were 11.6, 25.7, 14.8 and 0.8%/h for un-fractioned, A, B1 and B2 fractions, respectively. The values were statistically well estimated, assessed by high t-value (>12.9). The Kd of carbohydrate fractions in rice straw were 4.8, 21.1, 5.7 and 0.5%/h for un-fractioned, A, B1 and B2 fractions, respectively. Although the Kd of B2 fraction was poorly defined with a t-value of 4.4, the Kd of the other fractions showed tvalues higher than 21.9. The un-fractioned corn showed the highest Kd (18.2%/h) among the feeds tested, and the Kd of A plus B1 fraction was 18.7%/h. Soybean hull had a Kd of 6.0, 29.0, 3.8 and 13.8%/h for un-fractioned, A, B1 and B2, respectively. The large Kd of fraction B2 indicated that NDF in soybean hull was easily degradable. The t-values were higher than 20 except for the B1 fraction (5.7). The estimated Kd of soybean meal was 9.6, 24.3, 5.0%/h for un-fractioned, A and B1 fractions, respectively. A small amount of gas (5.6 ml at 48 ho of incubation) was produced from fermentation of CMLP which contained little carbohydrate. In summary, the automated gas production system was satisfactory for the estimation of well defined (t-value >12) kinetic parameters and Kd of soluble carbohydrate fractions of various feedstuffs that supply mainly carbohydrate. The subtraction approach, however, should be applied with caution for some concentrates, especially those which contain a high level of crude protein since nitrogen-containing compounds can interfere with gas production.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.7
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• pp.922-928
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• 2000

The effects of animal species and supplements on rumen fluid characteristics, plasma urea-N (PUN) concentration, plasma urea-N pool size, urea-N degradation in the gut and urea-N net flux (urea-N synthesis rate) were studied in goats and sheep, with some minor differences detected. The animals were fed either chopped rice straw ad libitum+200 g soybean meal (SBM), or chopped rice straw ad libitum+190 g soybean meal+300 g sago meal (SBM+SM) for 14 days. The supplements were isonitrogenous (80 g crude protein/animal/d). [$^{14}C$]-urea was used as the marker for urea metabolism studies. Two animals from each species were fed either supplement in a cross-over design in two periods. The results showed that rumen pH was significantly (p<0.001) lower in animals fed SBM+SM than those fed SBM supplement. The ammonia concentrations of rumen fluid were significantly (p<0.01) higher in sheep (382.9 mg N/L) than goats (363.1 mg N/L) when fed SBM supplement but lower (282.5 mg N/L) than that of goats (311.0 mg N/L) when fed SBM+SM supplement. Total VFA concentrations were significantly (p<0.05) higher in animals fed SBM+SM supplement than those fed SBM supplement. Goats had significantly (p<0.01) higher molar proportions of acetate (79.1, 77.7%, respectively) than sheep (75.8, 74.0%, respectively) in both supplements. The molar proportion of acetate was significantly (p<0.05) higher, while that of butyrate lower in animals fed SBM supplement than those fed SBM+SM supplement. In animals fed SBM supplement, the molar proportion of propionate was significantly (p<0.01) higher in sheep (18.0%) than in goats (15.6%), but in animals fed SBM+SM, the molar proportion of butyrate was significantly (p<0.01) higher (9.6%) in sheep than in goats (7.2%). Plasma urea-N concentration, plasma urea-N pool size, urea-N degradation in the gut, urea-N net flux and the fraction of urea-C from the blood entering the rumen were not significantly different between goats and sheep fed either supplement. However, PUN concentration was significantly (p<0.05) lower in animals fed SBM+SM supplement (average of 13.8 mg N/100 ml) than in those fed SBM supplement (average of 16.5 mg N/100 ml). The urea net flux was significantly (p<0.05) higher in goats (average of 14.5 g N/d) than sheep (average of 12.9 g N/d), and animals fed SBM supplement showed higher (average of 14.9 g N/d) urea net flux than animals fed SBM+SM supplement (average of 12.9 g N/d). A significant (p<0.05) positive correlation was observed between urea-N net flux and urea-N degradation; urea-N net flux and pool size; urea-N net flux and urea excretion in the urine; and PUN and rumen ammonia in goats. While in sheep, significant (p<0.05) positive correlation was observed between urea-N net flux and urea excretion in the urine; and PUN and rumen ammonia.

• Korean Journal of Environmental Agriculture
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• v.9 no.1
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• pp.1-8
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• 1990

The effects of soil environmental conditions on the degradation rates of fenitrothion(O-O-dimethyl O-4-nitro-m-tolyl phosphorothioate) in soils under flooded conditions were examined in the laboratory. Fenitrothion was degraded rapidly and the half life period was within 4 days. Furthermore the degradation was mere rapid under flooded conditions than under upland conditions. The decomposition rate was varied with soils and soil temperatures. Fenitrothion degraded more slowly at 30ppm than at l0ppm. Repeated applications of fenitrothion in soils accelerated the degradation rates. The degradation remarkably increased with amendment of rice straw. However, degradation rates ,were virtually unaffected by the addition of the mixed-fertilizer, the fungicide IBP and the herbicide butachlor. The population of fenitrothion-degrading microbes, which were counted by MPN method, always corresponded with the degradation rates in the soils.

• Journal of Life Science
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• v.19 no.3
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• pp.397-402
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• 2009

To isolate bacteria secreting protease, which can dissolve fibrin efficiently, we prepared chunggukjang using rice straw and isolated, preliminarily, approximately 100 bacterial stains. Their capabilities to dissolve milk protein as well as fibrin included in media were then examined and finally, five strains named J1 - J5 were selected. Among them, J-4, which is close to bacillus subtilis, showed highest activity for fibrin dissolution. Proteases secreted from the J-4 strain were partially purified from culture supernatant using DEAE-sepharose column chromatography and identified with SDS-polyacrylamide gel electrophoresis. Three proteins were subjected to analysis with MALDI-TOF and PMF (Peptide Mass Fingerprinting). 41.9 kDa protein was identified as a neutral protease. On the other hand, 45 kDa protein turned out to be bacillopeptidase F, with a molecular mass of 91.7 kDa, indicating that partially purified peptide is a degradation product.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.5
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• pp.648-654
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• 2004

This study was carried out to assess whether feeding of alcohol-fermented feeds (AFF) affects the nutritional metabolism and growth characteristics of Korean native steers. Ten steers were randomly assigned to one of two treatment groups. The dietary treatments were AFF (50% commercial beef cattle feed+30% alcohol-fermented soybean curd dregs+20% rice straw) and control (80% commercial beef cattle feed+20% rice straw). The change of serum metabolites and growth characteristics were measured every two months during the whole twelve months experimental period and the relationships between serum metabolites and growth characteristics were simultaneously analyzed. Four hours after feeding AFF, serum alcohol concentration reached its peak with a significantly higher value than that after control feeding (11.9 and 4.9 mg/dl, respectively). Serum glucose and inorganic phosphorus (IP) concentrations (63.1 and 8.4 mg/dl, respectively) of steers fed AFF were higher than those (56.6 and 7.0 mg/dl) fed the control diet. In both treatments, the serum glucose concentration rapidly increased when body weight (BW) of the steer reached about 600kg, while IP concentrations were rapidly diminished at that BW. Lower concentrations of both blood urea nitrogen (BUN) and cholesterol were observed in steers fed AFF up to 450 kg of BW. The IP concentration was correlated with concentrations of BUN, cholesterol and glucose in AFF fed cattle but not in the cattle fed control diets. Average daily gain was higher in steers fed AFF than steers fed control, particularly during the growing stage of cattle. These findings indicated a capability of AFF to improve BW gain of Korean native steers by decreased protein degradation as well as increased fat synthesis.