ALA (5-aminolevulinic acid) has been proposed as a tetrapyrrole-dependent photodynamic herbicide and insecticide by the action of the protoporphyrinogen IX oxidase (Protox IX). The present study was conducted to determine growth responses of plant and insects to ALA, biodegradable biopesticidal substance. In the paddy condition experiment, plant height and shoot fresh weight of barnyardgrass (Echinochloa crus-galli) was more reduced by ALA than rice plants, even though both plant species show great phytotoxicity. Hairy crabgrass (Digitaria sanguinalis), a monocot weed, was more sensitive to ALA at 5mM under upland condition when ALA applied on the foliage, compared with soybean (Glycine max) as a dicot crop. ALA solutions were tested for their insecticidal and larvicidal activities against Spodaptera exigua (Hubner) and Tetranychus urticae Koch. by foliar application and leaf-dipping method. The result showed higher insecticidal activity of ALA at 10mM and its mixture with insecticide luferon against S. exigua. Strongest insecticidal activity against T. urticae was observed from the ALA solution at 10mM 72 days after application. This results show that ALA solution had potent herbicidal and insecticidal activities against agricultural pests even though their activities were lower than those of synthetic pesticides.
The effects of soil environmental conditions on the rate of degradation of fungicide IBP (Iprobenfos, S-benzyl O, O-diisopropyl phosphorothioate) in the soils under flooded condintions were examined in the laboratory. IBP in soil was degraded more slowly under flooded conditions than under upland conditions. The degradation greatly varied among soils, and the degradation rate was negatively correlated with the content of soil organic matter. Degradation of IBP was influenced by the soil temperature and the amount of IBP applied. The rate of degradation in soil was remarkably inhibited by the amendment of rice straw but not affected by the treatment of mixed-fertilizer, and insecticide fenitrothion and herbicide butachlor. The degradation of IBP was assumed to be due to microorganisms, especially aerobic microbes, as no degradation was observed in sterilized soil.
Butachlor incubated for certain periods of time under the simulated, rather aerobic, rice-paddy conditions of two different soils exhibited two major degradation products. 2,6-diethyl-N-(butoxymethyl) acetanilide and a new product C in all cases, and a relativly small amount of another new product D named tentatively as 8-ethyl-2-hydroxy-N-(butoxymethyl)-3,4-dihydroquinoline in some cases. The supposedly microbial degradation seemed to proceed with incubation periods to some extent. An anaerobic incubation in the previous investigation showed 2,6-diethyl-N-(butoxymethyl) acetanilide as the major product, whereas the new product C with m/z 291 turned out to be the major one in the present condition. Structural elucidation of the products was done based on the fragmentation patterns of the given mass spectra and a possible pathway for their formation was postulated.
2,4,5-trichlorophenoxyacetic acid (2,4,5-T)-degrading bacterial strains were isolated from rice field and field in suburbs of Taejon. Of the total 100 isolates, 19 strains were selected by fast growth on solid minimal media containing 2,4,5-T as a sole of carbon and energy, and they were identified to genus level. 11 strains were identified as Pseudomonas, 4 strains as Acinetobacter, 1 strains were as Alcaliagenes and 3 strains were not identified. Strains MU19 and MU92 which were identified as Pseudomonas were capable of degradation for 4 kinds of chlorinated aromatic hydrocarbons, 2,4-D, 2,4,5-T, MCPA and 3CB. Acinetobacter sp. MU38 showed the highest degradability in liquid minimal media at 48 hours after inoculation, and Pseudomonas spp. MU19. MU57, MU73, and MU92 were able to degrade carbon source at higher rates. As the results Acinetobacter sp. MU38 and Pseudomonas spp. MU19 and MU92 were capable of biodegradation for broad range of halogenated aromatic hydrocarbons, and had higher rates of degradation for 2,4,5-T.
Three varieties of Echinochloa crus-galli (L.) Beauv. and five collections different in gross morphology from the E. crus-galli species were obtained in a lowland rice field located in Jukjeol-Ri, Soyang-Myeon, Wanju-Kun, Jeonbug to compare the ecological and physiological characteristics. There were great variations in seed dormancy, relative growth rates, days required to panicle emergence and panicle morphology among the collections. On the basis of the cluster analysis using the different characteristics, the collections were divided into three groups; E. crus-galli var. frumentaceae and its two ecotypcs, E. crus-galli var. echinata and its two ecotypes, and E crus-galli var. crus-galli and its one ecotype. There were differential responses in percent germination and post-germination growth of the collections to butachlor (2-chloro-2', 6'-diethyl-N-butoxymethyl acetanilide).
Proceedings of the Korean Society of Crop Science Conference
/
2022.10a
/
pp.167-167
/
2022
Seed production of italian ryegrass has a problem of lodging during ripening and a decrease in quality due to difficulty in drying seeds during harvest. Therefore, in order to produce high-quality Italian ryegrass in paddy fields, it was carried out to reduce the density and solve the lodging problem through transplanting. In this experiment, Lolium multiflorum cv. Kowinearly was transplanted in autumn from a paddy field in Sindong-ri, Gwansan-eup, Jangheung-gun, Jeollanam-do. var. Kowinearly was made into a bed at 90 g/box and stacked in boxes. It was transplanted on October 27th after 2 days of germination at 30℃ and 15 days of seedling and greening. When transplanting, they were transplanted at intervals of 30×14cm. The existing cultivation method, drill seedling, was sowed at a level of 50 kg/ha, and both transplanting and drilling were carried out at a nitrogen fertilization rate of 45 kg/ha. The number of ears during transplant cultivation was 1,016/m2 and the drilling tended to be higher at 2,278/m2, but this was probably due to the difference in seeding amount. The seed number of an ear tended to be 56% higher in transplantation, which had a significant impact on yield. The seed yield was 2,096 ka/ha in transplantation, which was 21% higher than that of drilling. When looking at the relationship with weed occurrence, there were areas where all the weeds, such as amul foxtail, occurred due to the low density. Even in the same transplanting area, the seed yield was about 1,000kg/ha less in the area where the weeds were abundant. It seems that weed management is important in paddy cultivation. Therefore, it seems necessary to develop an exclusive herbicide for Italian ryegrass cultivation.
Six herbicides were evaluated to investigate the phytotoxicity of rice plant and the weeding efficacy influenced by the time of application in mechically transplanted paddy field. The amount of each chemical applied was 3 kg, a. i, /ha. Chlormethoxynil : Rotala indica KOEHNE and Lindernia pyxuiaria PHILCOX were effectively controlled when applied on the 12th day after transplanting (12 DAT) and this herbicide was excellent for the control of Echinochloa crusgalli P. BEAUV, Monochoria vaginalis PRESL and Sagittaria pygmaea MIQ, when applied early (7 days after puddling) but its weeding effect for these weeds decreased greatly as the application time became later. It had a controlling effect for Potamogeton distinctus A. BENN, Cyperus serotinus ROTTB and Scirpus hotarui ROXB at the initial period at the earlier application time. Butachlor was effective in controlling E. crusgalli, R. indica and L. pyxidaria at 12 days after transplanting (DAT) but was not effective in controlling P. distinctus and S. pygmaea even at the early application time. M. vaginalis, C. serotinus and S. hotarui were effectively controlled by the butachlor treatment at 7 days after final puddling (2 DBT-SDAT) but this weeding effect decreased at the late application time. A combination of butachlor and naproanilide excellently controlled E. crusgalli, R. indica, L. pyxidaria and S. pygmaea regardless of the application time. For the control of M. vaginalis, C. serotinus and P. distinctus, the weeding effect of this mixtures was much greater than that of the single treatment of butachlor. Perfluidone was excellent for the control of E. crusgalli, R. indica, L. pyxidaria, M. vaginalis and S. pygmaea at either application time tested. P. distinctus, C. serorinus and S. hotarui could be controlled by this chemicals until the time of first observation (23 DAT) but the effect for these weeds somewhat decreased as time passed. The effect of pyrazolate on E. crusgalli, M. vaginalis, S. hotarui and P. distinctus was very excellent regardless of the application time but R. indica and L. pyxidaria could not be completely eliminated by this chemical. This chemical was effective in controlling C. serotinus when applied at 7-9 days after final puddling and showed a controlling effect for S. hotaruionly at the initial period. Piperophos + dimethametryn was very excellent for the control of all the annual weeds and P. distinctus. It showed a controlling effect on S. pygmaea, C. serotinus and S. hotarui only at the initial period. There was no difference in the effects on phytotoxicity and yield between chlormethoxynil and pyrazolate at either times of application tested. The later the application time was, the less the phytotoxicity of butachlor and piperophos+dimethametryne was. The phytotoxicity of butachlor + naproanilide and perfluidone decrease in the plots treated at the later application time. When the last two chemicals were treated at 2 days before transplanting (DBT) the yield decreased as compared with the hand weeded plot.
Weed flora and floristic composition were reviewed in lowland rice field and upland crop area. For lowland rice field weed flora was not much changed since 1971. About 29 weed species belonged to 18 families were occurred. However, floristic composition of dominant weed species has greatly changed mainly due to introduction of herbicides. The predominant weed species in 1971 when herbicide was not used were Rotala indica, Eleocharis acicularis, Monochoria vaginalis, Echinochloa crus-galli, while these for in 1991 were Eleocharis kuroguwai, Sagittaria pygmaea, S. trifolia, Echinochloa crus-galli and M, vaginalis, respectively. In 1981 weed survey, E. crus-galli was no longer troblesome weed. However, this species became important again thereafter by introduction of herbicide mixtures with pyrazolate, bensulfuron-methyl or pyrazosulfuronethyl. For upland crop area, 216 weed species belonged to 46 families were recorded. One hundred and sixtyfive of these were grown in winter crop area while 189 weed species occurred in summer crop area, respectively. Among these, 138 weed species were grown in both crop seasons. In general, summer crops had less number of weed species compared to winter crops. Even though the dominant weed species varied by crop the most common weeds were Chenopodium album, Alopecunrs aqualis, Stellaria alsine and S, media for winter crops and Digitaria sanguinalis, Portulaca oleracea, Chenopodium album and Acalypha australis for summer crops, respectively.
Park, Byung-Jun;Kwon, Oh-Kyung;Kim, Jin-Kyoung;Kim, Jin-Bea;Kim, Jin-Ho;Yoon, Soon-Kang;Shim, Jae-Han;Hong, Moo-Gi
Korean Journal of Environmental Agriculture
/
v.28
no.2
/
pp.194-201
/
2009
To evaluate the exposure of non-point source pesticide pollution in agricultural watershed and to investigate pesticide distribution and runoff from agricultural land, paddy field, upland and orchard, this experiment was carry out during crop growing seasons. The pesticide were detected twenty pesticides fungicide 4, insecticide 10, herbicide 6) in water of Neungchon agricultural watershed and detection concentrations were range 0.008${\sim}$7.59 ppb. Most of the detection pesticides were using pesticides to rice paddy fields to control fungi, insects, weeds. During the crop cultivation, the pesticide were detected total thirty pesticides by pepper field soil 6, orchard soil 4, sesame field soil 3 and rice paddy field soil 5, and pesticide concentrations were range 0.001${\sim}$0.109 ppm. Especially the herbicides were detected mainly in May and June in the stream water. The pesticide were detected thirty pesticides by fungicide 2, insecticide 6, herbicide 5 in water of Jungam Koseong agricultural watershed and detection concentrations were range 0.01${\sim}$7.21 ppb. In regard to the detected pesticides, the concentration of individual pesticides measured in surface water of the study areas never exceeded guidelines for agriculture chemicals concerning water quality-effluent from paddy fields in Japan (Katayama, 2003). Runoff rate of pesticides was range 0.07${\sim}$3.06 % from Kongju agricultural land to watershed after applied pesticides.
Kim, Man-Ho;Ryang, Kwang-Rok;Lee, Chang-Hyeuk;Shim, Jae-Weon;Kim, Kyung-Hyun;Yoon, Cheol-Su;You, Yong-Man;Pyon, Jong-Yeong
The Korean Journal of Pesticide Science
/
v.9
no.4
/
pp.401-410
/
2005
A series of studies involving formulation processes, bubbling activity test, diffusibility test and biological efficacy test was undertaken to develop Jumbo herbicide formulations in paddy rice field. Gas evolution speed from the tablets prepared by different organic acids was in the order of oxalic acid, malonic acid, citric acid, and tartaric acid. The total volume of evolved gas from the tablet and diffusibility of the active ingredient in the submerged water were increased with increase of water temperature; the volume from 1 g of tablet at 10, 15, 25 and $30^{\circ}C$ for 5 minutes after immersion into water was 20, 25, 28, 45, 57 mL, respectively. The concentration of halosulfuron-methyl and pyriminobac-methyl in submerged water at 5, 15, 20 and $30^{\circ}C$ at the 2.4 m distance from the applied spot of the tablet was 20, 48, 85, and 97% of the concentration of treated spot, respectively. The evolved gas volume from the tablets was not affected by pH of submerged water. The concentration of halosulfuron-methyl in different sizes of submerged water within 24 hours after treatment of the tablet was maintained 0.16 ppm, which is ideal concentration at standard dosage regardless of the submerged water area. The concentration of pyriminobac-methyl was also uniformly dispersed in the water within 24 hours after applying it into the submerged water. The wind velocity of 5 m $sec^{-1}$ on concentration distribution of halosulfuron-methyl and pyriminobac-methyl in the submerged water 24 hours after treatment was not influenced; an equal concentration in the up the wind and down the wind from the applied spot was maintained. Spot treatments of one tablet formulations(5 g) including 4 times higher dosage at 4 different spots resulted in even concentration distribution of active ingredient in the water 24 hours after applying it into the submerged water.
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