• 한국토양비료학회지
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• 제31권2호
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• pp.158-161
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• 1998

본 연구는 벼의 저온발아중 생채내의 ${\alpha}$-amylase 활성, 유리 proline함량, 생육특성 등 몇가지 실험을 수행하였던 바 얻어진 결과를 요약하면 저온발아시 초장은 품종간에 차이를 보이지는 않았지만 저온발아성이 다소 강한 대야벼가 3.9cm로 가장 우세하였으며 근장은 동진벼에서 4.8cm로 매우 양호한 생육현상을 보이는 것으로 나타났다. 벼 품종간 발아일수에 따른 ${\alpha}$-amylase 활성은 발아 후 3일째 고시히까리가 가장 낮은 65.4 mg인 반면에 동진벼는 80.9 mg 으로 매우 높은 활성을 보였다. 유리 proline 함량변화는 발아 후 3일째 저온발아능력이 다소 약한 품종인 상주벼와 고시히까리가 각각 $1.807{\mu}mole$, $1,800{\mu}mole$인 것보다 다소 강한 품종인 동진벼와 대야벼가 각각 $1,933{\mu}mole$, $1,895{\mu}mole$로 높은 함량변화를 보였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.192-192
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• 2017

Direct-seeding cultivation of rice is increasing in Asia instead of transplanting system, because of its lower cost and operational simplicity. Low-temperature germinability (LTG) and anaerobic germinability (AG) are important characters for breeding of varieties for wide-spread adoption of direct-seeding cultivation in rice. This study was performed to characterize LTG and AG of seven rice varieties and identify varieties with strong germinability on both low-temperature and anaerobic conditions. The mean germination rate and germination vigor of seven varieties were 51.7% and 6.0 under low-temperature condition, respectively. Among these varieties, Cheongcheongjinmi and Hwanggeumnodeul had the highest germination rate of 80%, indicating that Cheongcheongjinmi and Hwanggeumnodeul have a good LTG. In anaerobic conditions, the germination rate and coleoptile length for all varieties were 47.6% and 3.2 cm, respectively. Of them, the highest germination rate and coleoptile length were observed in Subo and Hopum, respectively, suggesting that these two varieties are tolerant to anaerobic during germination stage. Molecular characterization by SDS-PAGE revealed that the protein patterns differed at 50 kDa, 40 kDa, and 22 kDa between low-temperature and anaerobic conditions. Varieties identified as good LTG or AG in this study may be used for developing new direct-seeding rice cultivars through pyramiding these traits in the breeding program.

• Food Science and Biotechnology
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• 제16권5호
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• pp.765-770
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• 2007

To examine the possibility of using rough rice as a health-functional food, we investigated its changes in biological activity with germination. Antioxidant activities of the 70% ethanolic extracts of 'Goami2', 'Keunnunbeyo', and 'Heugkwangbeyo' were studied in comparison with those of ungerminated rough rice. The phytic acid level in rough rice decreased on germination, while the level of phenolic compounds increased. Reducing power increased in a dose-dependent manner and the germinated rough rice tended to have enhanced reducing power. Among the rough rice cultivars, the germinated 'Heugkwang' rough rice tended to be the most effective, with scavenging activities for the DPPH, superoxide, and hydroxyl radicals that were 1.6-, 1.3-, and 1.6-fold greater than those of the ungerminated 'Heugkwang' rough rice, respectively. We also found that the germination process increased antioxidant activity in all the rough rice cultivars, where activity was greatest for the 'Heugkwang' rough rice cultivar.

• 한국작물학회지
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• 제59권4호
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• pp.427-434
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• 2014

1. 생산지에 따라 벼 종자의 수발아립률 차이가 컸던 호품벼의 출아율과 발아율을 조사한 결과, 수발아율 8%인 시료는 수발아율이 1%인 시료에 비해 발아율과 출아율, 종자활력이 30% 낮았다. 2. 수발아율이 높은 시료는 수발아립의 변색 정도가 심하고 미발아율과 부패율이 높았다. 수발아립은 배가 돌출하고, 변색된 배유와 곰팡이 감염이 관찰되었으며 배유의 1/2이상이 변색된 종자는 발아율이 매우 낮았다. 3. 종자의 발아 진행정도에 따라 현미의 변색이 심해졌는데, 싹이 3 mm이상일 경우 배유의 1/2정도가 변색되었다. 4. 발아 종자는 싹이 3 mm이상일 때부터 재발아율이 크게 떨어졌으며 퇴화촉진처리 후 발아율의 감소가 더 컸다. 특히 퇴화촉진처리한 발아 종자는 싹이 2 mm부터 종자활력이 크게 감소하였다. 결론적으로 수발아 발생이 심할수록 파종기 발아율과 출아율의 감소가 크고, 수발아가 3 mm이상 발생하면 발아율이 크게 저하될 것으로 생각된다. 또한 수발아는 동일 품종과 동일 지역에서도 포장 간 차이가 크게 발생할 수 있으므로 재배관리에 주의해야 한다.

• Journal of Biosystems Engineering
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• 제35권2호
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• pp.100-107
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• 2010

This study was conducted to investigate the effects of electric current stimuli and high-voltage electric field treatments on brown rice germination. The brown rice stimulated by electrical current stimuli, functional electrical stimuli of a pulse type, and high-voltage electric field treatments were observed (Type I, II and III). Treatment Type I was a method of semi-soaking brown rice with electric current stimuli of 0.13 V/cm, 0.19 V/cm, and 0.25 V/cm into Petri-dishes for 72 hours. Type II was a method of semi-soaking brown rice with functional electrical stimuli of a pulse type(DC 1 V, 1 Hz, 5%, and duty cycles of 5%, 20%, and 35%) into Petri-dishes for 72 hours. Type III was a method of water-soaking with high-voltage electric field treatments for 60 hours. High-voltage electric field treatments at 15 kV/cm were also conducted for 2.5 min, 7.5 min, and 10 min, respectively. The germination rate and the sprout growth of brown rice germinated by electric current stimuli with 0.13 V/cm, 0.19 V/cm, and 0.25 V/cm were increased by about 10-15% compared with those of the control group. The germination rate and the sprout growth of brown rice germinated by functional electrical stimuli of pulse type(DC 1 V, 1 Hz, 5% duty cycle) were increased by about 10∼15% compared to those of the control group. Also, the best effective treatment among high-voltage electric field treatments was the 10 min group at 15 kV/cm. The germination rate and the sprout growth of brown rice germinated by this treatment of 10 min at 15 kV/cm were increased by about 10∼20% compared to those of the control group. The treatments of electric current stimuli and high-voltage electric field accelerated the germination rate and sprout growth of brown rice by about 10∼15% compared to those of the control group.

• 한국작물학회지
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• 제52권1호
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• pp.29-35
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• 2007

국내 및 일본에서 육성된 벼 품종을 고온 저장시 저장기간에 따른 발아율, 이화학적 특성, lipoxygenase의 활성 및 이들 상호관계를 검토하였다. 1. 고온저장($35^{\circ}C$)시 발아율, 현미 단백질, 지방산도 및 lipoxygenase 활성은 저장기간 및 품종 간 차이가 크게 있었으며 두 요인의 상호작용도 인정되어 저장기간별 품종 간 차이가 다르게 나타났다. 2. 고온저장에 의한 현미 단백질, 지방산도 및 lipoxygenase 활성은 저장기간이 길수록 증가하였던 반면, 발아율 및 도요식미치는 감소하는 경향이었다. 3. 지방산도와 발아율 간에는 부의 상관이 지방산도와 lipoxygenase의 활성은 정의 상관이 인정되었다. 4. 17개 품종을 발아율, 지방산도 및 lipoxygenase 활성을 고려하여 군집분석을 수행한 결과 2개의 그룹으로 구분되었다. I군에 속하는 품종은 오대벼, 상미벼, 운광벼, 일품벼, 남평벼, 일미벼, 동안벼, 중산벼, 추청벼, 히또메보레, 고시히까리 등 11개 품종으로 비교적 발아율이 높고 지방산도 및 lipoxygenase 활성이 낮았다.

• 한국식품영양과학회지
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• 제40권9호
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• pp.1265-1270
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• 2011

본 연구에서는 벼의 적당한 발아기간을 선정하기 위하여 발아기간에 따른 다양한 성분변화를 살펴보았다. 조단백질은 발아 0일차의 71.67 mg/g에서 발아 8일차에는 85.20 mg/g으로, 조지방은 2.19%에서 3.58%로 발아기간이 증가할수록 증가하였으나(p<0.05) 조회분은 큰 차이를 보이지 않았다. Phytic acid는 발아가 진행됨에 따라서 6.25 mg/g(0일차)에서 1.54 mg/g(8일차)으로 많은 감소를 보였으며(p<0.05), 유리지방산은 0.17%에서 0.32%로 증가하였다. 발아 벼의 지방산은 oleic acid(C18:1)가 가장 많았으며, 발아기간의 증가에 따라 약간의 감소를 보였으나 유의차는 없었다(p>0.05). 비타민 E와 ${\gamma}$-oryzanol은 발아 0일차에 각각 2.94 mg/100g 및 6.47 mg/g에서 발아 4일차에 5.55 mg/100 g 및 8.16 mg/g으로 증가하였다가 그 이후에는 감소하였다. 본 연구 결과 기능성성분 함량을 높이기 위해서는 3~4일 간의 발아가 적당한 것으로 판단되며, 다양한 생리활성에 대한 추가적인 연구가 필요하리라 판단된다.

• 한국작물학회지
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• 제51권1호
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• pp.53-58
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• 2006

This study was conducted to investigate the crossability, seed dormancy and overwintering ability of rice plant in GM (glufosinate ammonium-resistant lines. Iksan 483 and Milyang 204) and non-GM (their parents) or red rice (Andongaengmi). Seed-setting rate was not significantly different between GM and non-GM rice varieties. Iksan 483 and Milyang 204 showed the similar level of seed germination rate from 30 to 50 days after heading as compared to non-GM rice varieties. After overwintering in paddy field, seed germination rate of GM and non-GM rice varieties ranged from 14.3 % to 57.6 % in dry soil condition, but there was no germination in wet-soil except red rice. The result in wet-soil condition may help to set up a strategy for reducing the risk of gene flow of transgene via dispersal of seeds of GM plants. The crossability, seed dormancy and seed overwintering of Iksan 483 and Milyang 204, herbicide resistant GM rice varieties, were not significantly different compared to non-GM rice varieties. The results might be helpful to reduce the risk of transgene dispersal from GM crop via seeds and pollens.

• KSBB Journal
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• 제17권6호
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• pp.515-519
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• 2002

발아현미를 생산하기 위하여 현미를 물에 침지, 키토산을 젖산에 용해하여 침지, 키토산을 글루탐산에 용해하여 침지 하였으며, 발아시키지 않은 현미와 아미노산 및 총 단백질 함량을 비교 분석하였다. 키토산을 50 ppm 되게 5 mM glutamic acid에 용해하여 침지액으로 사용한 경우 가장 높은 alanine, serine, lysine, isoleucine, methionine 함량의 증진과 총 유리아미노산 함량의 증진을 보였다. 또한 total soluble protein의 함량은 발아하지 않은 현미에 비하여 발아한 현미가 모두 낮았다. 특히 CG구는 물발아나 CL 발아시 현저히 감소되던 serine의 함량을 오히려 증진시겼다. 모든 발아구에서 aspartic acid 함량은 현저히 감소하였다. 이는 발아 과정에 의해 aspartic acid가 alanine, lysine, isoleucine, methionine 등으로 전환된 것에 기인된 것이라 여겨진다. 이상의 결과를 종합하면 현미발아시 키토산을 글루탐산에 용해하여 침지액으로 사용하면 유용한 아미노산인 alanine, serine 및 필수아미노산인 lysine, isoleucine, methionine 함량을 현저히 증진시킬 수 있어 영양성이 보강된 발아현미를 얻을 수 있을 것으로 기대된다.

• 한국육종학회지
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• 제42권5호
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• pp.439-447
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• 2010

Riptortus clavatus, one of the many insects in major crops, damages pods and seeds, which reduces seed vigor and viability in soybeans. This study was conducted to examine the effect of diversely damaged seeds by R. clavatus on seed germination and seedling emergence and to determine the association of damaged seed with quality and yield of soybean sprouts. All seeds damaged by R. clavatus significantly (P<0.05) reduced seed vigor as measured by the rates of seed germination, germination speed, and seedling emergence. Mean seed germination rate of non-damaged seeds in sprout-soybean varieties was 97.8%, whereas the rates of seeds damaged at different levels, 31-50% and 51-80%, were 23.0 and 5.4%, respectively. The rates of seedling rot and abnormal, incomplete germination significantly (P<0.05) increased as the amount of seeds damaged by R. clavatus increased to 5, 10 and 15% against the total seeds for sprout production. Yield of soybean sprouts from seeds damaged at different levels decreased up to 13% as compared to that in normal seeds. In customer preferences on soybean sprout produce, 84% of customers participated in survey preferred to purchase sprouts from seeds with 5% of damaged seeds, but sprouts produced from seeds with 15% of damaged seeds were intended to purchase only by 22% of the customers. Areas of the seed damaged by R. clavatus were readily infected by pathogens as the seed germinated, resulted in deteriorated quality and reduced yield of sprout produce.