• 제목/요약/키워드: rice genetics

검색결과 108건 처리시간 0.026초

수도체의 함유성분과 도열병 저항성에 관한 연구 (Studies on the Relationship Between Chemical Contents of Rice Plants and Resistance to Rice Blast Disease)

  • 권신한;오정행;송희섭
    • 한국응용곤충학회지
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    • 제13권1호
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    • pp.33-39
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    • 1974
  • 통일품종이 가지는 도열병 저항성의 요인을 구명하기 위하여 수도체내에 함유하는 질소, 규산 및 Flavonoid함량을 분석하고 시비수준을 달리하여 재배한 풍광에서 이들 성분함량이 도열병 발병율에 미치는 영향을 조사하였다. 1. 수도체내 규산과 Flavonoid 함량은 질소 시비량과 반비례하고 규산 시비량과는 정비례하여 증가하였다. 2. 도열병 상습답에서의 도열병 발병율은 체내 질소함량에 정비례하고 규산함량에 반비례하였다. 3. 도열병에 대하여 저항성인 통일에서는 저항성의 요인이 되는 규산 및 Flavonoid의 체내함량이 이병성인 풍광에 비하여 현저히 높았다. 4. 규화세포의 수는 고엽일수록 많고 생육의 진전에 따라 증가하였으며 풍광에서 보다는 통일에서 현저히 높았다. 5. 이들 체내 성분의 함량은 통일이 갖는 도열병 저항성의 한 요인인 것으로 보였다.

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Genomic Differentiation Among Oyster Mushroom Cultivars Released in Korea by URP-PCR Fingerprinting

  • Kang, Hee-Wan;Park, Dong-Suk;Park, Young-Jin;You, Chang-Hyun;Lee, Byoung-Moo;Eun, Moo-Yong;Go, Seong-Joo
    • Mycobiology
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    • 제29권2호
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    • pp.85-89
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    • 2001
  • URP primers of 20 mer derived from repetitive sequence of rice were used to assess genetic variation of oyster mushroom consisting of 10 cultivars of Pleurotus ostreatus, two cultivars of P. florida and two cultivars of P. sajor-caju which were registered in Korea. URP2F and URP38F primers produced cultivar-specific PCR polymorphic bands in the Pleurotus species. UPGMA cluster analysis using the URP-PCR data showed that 14 Pleurotus cultivars are genetically clustered into large three groups. The URP-PCR data provided important information for more efficient breeding strategies of Pleurotus cultivars.

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Effect of Water Extract of Germinated Brown Rice on Adiposity and Obesity Indices in Mice Fed a High Fat Diet

  • Oh, Suk-Heung;Moon, Yeon-Jeong;Soh, Ju-Ryoun;Cha, Youn-Soo
    • Preventive Nutrition and Food Science
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    • 제10권3호
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    • pp.251-256
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    • 2005
  • An extract obtained from germinated brown rice was evaluated for protective effects against the adverse effects of a chronic high fat diet. C57BL6/J mice were divided into three groups; normal diet + water group (ND), high fat diet + water group (HD), high fat diet + brown rice water extract group (HD + BE) and fed for 8 weeks (6 day/week). The ND group diet was 11 kcal fat $\%$. The HD group and HD + BE group diet were 42 kcal fat $\%$. Weight gains were not significantly different between groups. However, abdominal fat $\%$ was 1.6-fold higher in the HD group than HD + BE group. Feed consumption was significantly higher in the ND group than HD group. Serum triglyceride, total cholesterol and LDL-cholesterol increased with a high fat diet, but decreased by brown rice water extract administration to the high fat diet group. Serum HDL-cholesterol decreased by the high fat diet, but increased by the brown rice wate extract administration. Also, serum HDL-cholesterol/total cholesterol ratio was 2.3-fold higher in the HD + BE than the HD. Liver triglyceride and total cholesterol were not significantly different among groups. These data suggest that brown rice water extract administration improves the serum lipid profiles of C57BL/6J mice.

Possibility of breeding super rice cultivars using gene linkage

  • Tran, Dang Xuan;Tran, Dang Khanh;Truong, Thi Tu Anh
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.27-27
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    • 2017
  • The rapid growth of world population, reduction of cultivated areas for crop production, and detrimental effects of pests, diseases, and climate changes have required to breed new rice cultivars with high yield, accepted quality, but strong resistance to abiotic and biotic stresses. However, traditional breeding needs much time to breed a new cultivar, whereas the successful use of molecular breeding is still questionable. We have developed a novel mutation which allow to cross many rice cultivars together with low segregation, that allow to breed a new cultivar in only several cropping. The mechanism has been unknown, but we suggest that gene linkage may play a crucial role, of which the semi dwarf gene might be the center gene for gene linkage occurrence. The phenomenon of this possible gene linkage is contrary to Mendel rules, but it is promising to breed new rice cultivars, of which, the most elite genes in rice might be able to gather in a targeted rice variety.

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Zinc finger RING-H2 protein관련 Ac/Ds전이인자 삽입 변이체 Oszinc626 유전자의 특성 분석 (Characterization of Oszinc626, knock-out in zinc finger RING-H2 protein gene, in Ac/Ds mutant lines of rice(Oryza sativar L.))

  • 박슬아;정유진;안병옥;윤도원;지현소;박용환;은무영;서석철;이순열;이명철
    • Journal of Plant Biotechnology
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    • 제35권3호
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    • pp.177-183
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    • 2008
  • 본 연구는 동진벼 유래의 Ac/Ds 삽입변이집단의 GUS 분석을 통하여 뿌리 및 미숙종자에서 강하게 GUS가 발현한 개체를 선발하여 FST(flanking sequence tag) 분석 한 결과 Ds 전이 인자가 3번 염색체 zinc finger RING-H2 관련 Oszinc626 유전자의 첫 번째 exon 부위에 single copy로 삽입되어 있었으며, 선발변이체는 뿌리 및 종자 발달이 정상인 동진벼에 비해 매우 낮은 것으로 나타났다. Oszinc626 유전자는 RING-H2 type(C3-H2-C3)으로 $Cys-X_2-Cys-X_{28}-Cys-X-His-X_2-His-X_2-Cys-X_{14}-Cys-X_2-Cys$ 배열이 C-terminus 가장 말단에 위치하며, 49 kDa의 분자량을 가지고 있다. 또한 Southern blot 분석에서 Oszinc626 유전자는 벼 게놈상에 single copy로 존재하였다. RT-PCR을 통한 돌연변이 유전자의 발현분석 결과 250 mM의 염과, $4^{\circ}C$ 저온등과 같은abiotic stress에 의해 발현이 증가함을 보였고, 호르몬처리에 있어서 ABA와 IAA의 식물호르몬을 처리했을 경우 24시간까지 계속해서 발현양이 증가하는 것을 보이는 반면, 2,4-D 처리의 경우 30분 후에 발현이 일시적으로 증가되었으나 이후 발현이 급속히 감소한 것을 보였다. 벼의 조직 별 발현 검정에서 미성숙한 종자, 뿌리 분열조직 및 신초 등 주로 생장점 부위에서 강하게 발현되는 것을 보임에 따라 Oszinc626 유전자의 경우 식물의 생장에 관여하는 주동 유전자의 하나로 판단된다.

Indigestible Carbohydrate Contents and Physical Properties of Goami2 harvested at the Maximized Milling Quality

  • Choi, In-Duck;Son, Jong-Rok;Hong, Ha-Cheol;Lee, Jeom-Ho;Kim, Kee-Jong
    • Food Science and Biotechnology
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    • 제15권2호
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    • pp.254-259
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    • 2006
  • Milling qualities and indigestible carbohydrate fractions (ICF) depending on harvesting time of Goami2 (G2), mutant of Ilpum (IP) rice, was examined. Fifty days after heading (DAH) maximized head rice milling quality (57.69%) and ICF content ($5.09{\pm}0.36\;g/100\;g$). ICF contents and physical properties of G2 and IP at 50 DAH were compared. ICF of G2 was three times higher than that of IP ($1.61{\pm}0.09\;g/100\;g$). Parboiling treatment increased ICF of G2 to $7.18{\pm}0.16\;g/100\;g$. G2 showed lower water absorption index, which could lower pasting properties, but higher water solubility index, implying it contains more soluble components. Texture properties of G2 were different from those of IP, showing higher hardness, and lower adhesiveness and cohesiveness. Positive correlation was observed between ICF and hardness, but reverse correlation between ICF and cohesiveness.

Physico-chemical Properties of Giant Embryo Brown Rice (Keunnunbyeo)

  • Choi, In-Duck;Kim, Deog-Su;Son, Jong-Rok;Yang, Chang-Inn;Chun, Ji-Yeon;Kim, Kee-Jong
    • Journal of Applied Biological Chemistry
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    • 제49권3호
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    • pp.95-100
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    • 2006
  • Brown rice with a giant embryo (GE) was observed on the quality parameters of the enlargement of embryo, nutritional components, and physical properties, in comparison to normal embryo brown rice (NE). Also, the effects of germination on the quality parameters were examined. GE embryo was approximately 2.68 times larger than of NE rice. Total free sugars were significantly higher in GE rice (71.96 vs. 41.17 mg/100 g), and germinated rice increased in fructose, but decreased in sucrose and maltose. No significant difference in mineral contents was found in GE and NE rice and their germinated rice, whereas a significant increment was observed on reducing sugars and gamma-amino butyric acid (GABA) contents in GE rice. The lower water absorption index (WAI) of GE rice resulted in relatively lower pasting viscosity, whereas the increased WSI in germinated rice might be attributable to the significant increment of soluble components in GE rice.

Functional properties of an alternative, tissue-specific promoter for rice NADPH-dependent dihydroflavonol reductase

  • Kim, Joonki;Lee, Hye-Jung;Tyagi, Wricha;Kovach, Michael;Sweeney, Megan;McCouch, Susan;Cho, Yong-Gu
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.163-163
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    • 2017
  • A deletion analysis of the Oryza sativa dihydroflavonol reductase (DFR) promoter defined a 25 bp region (-386 to -362) sufficient to confer pericarp-specific expression of ${\beta}$ -glucuronidase(GUS) reporter gene in transgenic rice. Site-specific mutagenesis of these conserved sequences and subsequent expression analysis in calli which transiently expressed the mutated promoter::GUS gene showed that both bHLH (-386 to -381) and Myb (-368 to -362) binding sites in the DEL3 (-440 to 70) promoter were necessary for complete expression of the GUS gene including the tissue-specific expression of DFR::GUS gene. The GUS gene was expressed well in the mutated Myb (-368 to -362) binding site, but not as strong as in normal condition, implying that the Myb is also necessary to express GUS gene fully. Also, we found the non-epistatic relation between Rc and DFR. There were no changes of expression patterns GUS under the Rc and rc genotypes. Thus, DFR expression might be independent of the presence of functional Rc gene and suggested that Rc and Rd (DFR) share the same pathway controlling the regulation of flavonoid synthesis but not a direct positive transcriptional regulator of DFR gene.

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벼의 품종별 이앙시기가 미질 특성에 미치는 영향 IV. 지경위치별 미립의 외관특성과 미질특성의 변화 (Characteristics of Grain Quality at Different Transplanting Times among Rice Cultivars)

  • 고재권
    • 한국자원식물학회지
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    • 제11권2호
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    • pp.152-156
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    • 1998
  • This experiment was conducted to investigated the characteristics of grain appearance, chemical components. gelatinization, and palatability of cooked rice on each rachis branches as harvested from the transplanting plot of May 20 and June 5 in each two varieties of early-maturing and late-maturing types. In evaluation of rachis branches, the percentage of ripended grain and 1,000 grain weight were found to be high and heavy at upper part of panicle. The factors influencing palatability such as maximum viscosity and breakdown as gelatinization characteristics, and hardness, apringiness, cohesiveness and chewiness as texture characteristics of cooked rice were found to be high at upper part of panicle , indicating the parlability was favored more at upper part than at lower part of panicle due to the genetics and physiological metabolism of rice plants.

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