• Parasites, Hosts and Diseases
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• 제52권2호
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• pp.163-168
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• 2014

Wnt proteins are a family of secreted glycoproteins that are evolutionarily conserved and considered to be involved in extensive developmental processes in metazoan organisms. The characterization of wnt genes may improve understanding the parasite's development. In the present study, a wnt4 gene encoding 491amino acids was amplified from cDNA of metacestodes of Taenia solium using reverse transcription PCR (RT-PCR). Bioinformatics tools were used for sequence analysis. The conserved domain of the wnt gene family was predicted. The expression profile of Wnt4 was investigated using real-time PCR. Wnt4 expression was found to be dramatically increased in scolex evaginated cysticerci when compared to invaginated cysticerci. In situ hybridization showed that wnt4 gene was distributed in the posterior end of the worm along the primary body axis in evaginated cysticerci. These findings indicated that wnt4 may take part in the process of cysticerci evagination and play a role in scolex/bladder development of cysticerci of T. solium.

• 대한기계학회논문집B
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• 제41권7호
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• pp.445-453
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• 2017

본 연구에서는 RO용 고압 다단 펌프의 수력 부 성능에 대해서 연구를 수행하였다. 수력 부 설계는 크게 임펠러 설계와 레이디얼 디퓨저 설계로 나뉠 수 있다. 임펠러의 자오면 형상 변화에 따른 유동분포와 성능을 수치 해석적으로 연구하였으며, 임펠러 외경, 출구 폭, eye dia 등은 고정시킨 상태에서 반응 표면 기법을 이용하여 허브 및 쉬라우드 자오면 곡선을 변화 시키면서 성능을 최적화하였다. 해석결과 양정에 가장 큰 영향을 미치는 설계 변수는 ${\varepsilon}Ds$로 나타났으며 효율은 허브 입구 길이 및 쉬라우드 곡선이 가장 큰 영향을 주는 것을 알 수 있었다. 자오면 프로파일을 변경한 결과 기준모델(case 25)에 비해 약 0.5% 효율이 개선됨을 확인할 수 있었다.

• 대한의생명과학회지
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• 제10권4호
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• pp.385-389
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• 2004

A novel gene Jpk, originally isolated as a trans-acting factor associating with the position-specific regulatory element of murine Hox gene has been reported to be expressed differentially in the liver of diabetic animals. Therefore, in an attempt to develop a possible diagnostic marker and/or new therapeutic agent for the Diabetes Mellitus, we analysed the expression pattern of Jpk among organs of normal and diabetic Sprague-Dawley (SD) rats. Total RNAs were isolated from each organs (brain, lung, heart, liver, spleen, kidney, muscle, blood, and testis) of diabetic and normal rats in both normal feeding and after fasting condition. And then RT (reverse transcription) PCR has been performed using Jpk­specific primers. The Jpk gene turned out to be expressed in all organs tested, with some different expression profiles among normal and diabetes, though. Upon fasting, Jpk expressions were reduced in all organs tested except kidney, muscle and brain of normal rat. Whereas in diabetes, Jpk expressions were increased in all organs except heart, muscle and testis when fasted. Compared to the normal rat, the Jpk expression level in blood was remarkably upregulated (about 15-30times) in diabetic rat whether in normal feeding or fasting conditon, suggesting that the Jpk could be a candidate gene for the possible blood diagnostic marker for the Diabetes Mellitus.

• Asian-Australasian Journal of Animal Sciences
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• 제16권12호
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• pp.1725-1731
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• 2003

Effect of strategically supplemented pregnancy allowance was ascertained during different phases of gestation on the reproductive performance of does. Gravid does (18) were allotted to 3 dietary treatments HH, HL and LH in a completely randomized block design. All does were provided wheat straw ad libitum and supplemented with concentrate mixture at the rate of $20g/kgW^{0.75}$ from 0-60 days post-mating. Subsequently, HH group was given concentrate at the rate of $40g/kgW^{0.75}$ from 61 d post-mating to term. HL group was offered concentrate from 61-90 d post-mating and 121d post mating to term at the rate of $20g/kgW^{0.75}$ and from 91-120 d post-mating at the rate of $40g/kgW^{0.75}$. LH group was provided with concentrate from 61 to 120 d post-mating and 121 to term at the rate of 20 g and $40g/kgW^{0.75}$, respectively. Mean total dry matter and concentrate intake ($g/kgW^{0.75}$) was significantly higher on HH in comparison to comparable intake of HL and LH treatments, however, wheat straw followed the reverse trend. Haematological and biochemical parameters except serum glucose, total serum protein and A:G ratio did not differ significantly among dietary treatments. The gross gain in weight, products of pregnancy at 21 weeks of gestation, pregnant does average daily gain, birth weight of kids and survivability of kids were significantly lower in treatment HL in comparison to comparable values for HH and LH treatments. The net gain in gravid does was significantly higher in treatment HH than the comparable values obtained in HL and LH treatments. The last month of pregnancy was found to be nutritionally most sensitive period of gestation for native goats.

• Ocean and Polar Research
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• 제36권2호
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• pp.165-177
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• 2014

In an effort to investigate the structure and variability of the coastal current in the East Sea, a moored ADCP observation was conducted off Uljin from late May to mid-October 2006. Owing to the transition of season from summer to autumn, the features of the current and wind can be divided into two parts. Until mid-August (Part-I), a southward flow is dominant at all depths with a mean alongshore velocity of 4.2~8.9 cm/s but northward winds are not strong enough to reverse the near-surface current. During Part-II, a strong northward current occurs frequently in the upper layer but winds are predominantly southward including two typhoons that have deep-reaching influence. Profile of mean velocity has three layers with a northward velocity embedded at 12~28 m depth. The near-surface current of Part-II significantly coheres with winds at 4-8 day periods with a phase lag of about 12 hours. The modal structure of the current obtained by EOF analysis is: (1) Mode-1, having 83.6% of total variance, represents the current in the same direction at all depths corresponding to the southward North Korean Cold Current (NKCC). (2) Mode-2 (11.7%) reveals a two-layer structure that can be explained by the northward East Korean Warm Current (EKWC) in the upper layer and NKCC in the lower. (3) Mode-3 (2.6%) has three layers, in which the EKWC is reversed near the surface by opposing winds. This mode is particularly similar to the mean velocity profile of Part-II.

• Archives of Pharmacal Research
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• 제29권10호
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• pp.890-897
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• 2006

In inflammatory responses, induction of cytokines and other immune regulator genes in macrophages by pathogen-associated signal such as lipopolysaccharide (LPS) plays a crucial role. In this study, the gene expression profile changes by LPS treatment in the macrophage/monocyte lineage cell line RAW264.7 was investigated. A 60-mer oligonucleotide microarray of which probes target 32381 mouse genes was used. A reverse transcription-in vitro translation labeling protocol and a chemileuminescence detection system were employed. The mRNA expression levels in RAW264.7 cells treated for 6 h with LPS and the control vehicle were compared. 747 genes were up-regulated and 523 genes were down-regulated by more than 2 folds. 320 genes showing more than 4-fold change by LPS treatment were further classified for the biological process, molecular function, and signaling pathway. The biological process categories that showed high number of increased genes include the immunity and defense, the nucleic acid metabolism, the protein metabolism and modification, and the signal transduction process. The chemokine-cytokine signaling, interleukin signaling, Toll receptor signaling, and apoptosis signaling pathways involved high number of genes differentially expressed in response to LPS. These expression profile data provide more comprehensive information on LPS-target genes in RAW264.7 cells, which will be useful in comparing gene expression changes induced by extracts and compounds from anti-inflammatory medicinal herbs.

• Geomechanics and Engineering
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• 제33권5호
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• pp.477-486
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• 2023

In the studies on fault dislocation of tunnel, existing literatures are mainly focused on the problems caused by normal and reverse faults, but few on strike-slip faults. The paper aims to research the deformation and failure mechanism of a tunnel under strike-slip faulting based on a model test and test-calibrated numerical simulation. A potential faulting hazard condition is considered for a real water tunnel in central Yunnan, China. Based on the faulting hazard to tunnel, laboratory model tests were conducted with a test apparatus that specially designed for strike-slip faults. Then, to verify the results obtained from the model test, a finite element model was built. By comparison, the numerical results agree with tested ones well. The results indicated that most of the shear deformation and damage would appear within fault fracture zone. The tunnel exhibited a horizontal S-shaped deformation profile under strike-slip faulting. The side walls of the tunnel mainly experience tension and compression strain state, while the roof and floor of the tunnel would be in a shear state. Circular cracks on tunnel near fault fracture zone were more significant owing to shear effects of strike-slip faulting, while the longitudinal cracks occurred at the hanging wall.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.517-522
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• 1998

The cry1 gene content of a collection of Bacillus thuringiensis strains, which include new isolates from Malaysia and Indonesia, was determined by a multiplex PCR using a set of eight oligonucleotide forward primers specific to cry1Aa, cry1Ab, cry1Ac, cry1Ba, cry1Ca, cry1Da, cry1Ea, and cry1Fa genes, and two reverse primers, one specific to cry1Ab and the other common to the remaining cry1 genes. Two-thirds of the 59 strains screened were cry1 positive and contained one to four different genes. The cry gene profiles correlated well with toxicities of the strains to lepidopteran insects. The method can be used for rapid screening of a large number of new isolates as the total DNA extracted by boiling cells from single colonies can be used directly in the PCR. However, it is not suitable for follow-up monitoring of specific commercial strains after application in the field as the PCR product profiles of these strains could not be differentiated from those of new isolates.

• Bulletin of the Korean Chemical Society
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• 제33권2호
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• pp.489-491
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• 2012

An $^{125}I$-labeled resveratrol derivative 1 was synthesized. It was purified by reverse phase HPLC. Radiochemical purity of the product was more than 98%, and the yield was 35% (decay-corrected). Its biodistribution in tumorbearing mice was studied. The results showed that the highest radioactivity was located in intestine and stomach. The biodistribution profile suggests that compound 1 can be effectively used as a promising imaging probe for intestine and stomach.

• 대한치과의사협회지
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• 제55권4호
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• pp.296-302
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• 2017

The traditional orthognathic surgery treatment consists of three steps: preoperative orthodontic treatment, orthognathic surgery, and postoperative orthodontic treatment, and the average treatment period is usually two years. Also, patients with Class III malocclusion should spend more time getting their facial features worse during the decompensation process. However, most of the patients who want orthognathic surgery visit the chief complaints of appearance improvement, and resolve this address as soon as possible. The concept of $^{\circ}{\AE}$Surgery - First 'does not cause a facial imbalance caused by decompensation for the pre - operative correction period, and the patient can obtain an improved facial profile immediately after the operation. In addition, the correction period is shortened by Regional Acceleratory Phenomenon (RAP) after surgery. However, it is not applicable to all patients. Patients with severe crowding, severe curve of spee or reverse curve of spee, severe transverse discrepancy of the maxilla and mandibular arch, and severe incisal angles are less likely to apply the technique. Although it is not yet possible to apply this technique to all patients, it has many advantages over the conventional method. Especially, the patients' preference is increasing due to the rapid appearance improvement and the shortening of the total treatment period.