• Journal of Yeungnam Medical Science
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• v.11 no.1
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• pp.115-126
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• 1994

The recent availability of recombinant human erythropoietin has opened new perspectives in the management of a variety of anemias. Clinical trials have been initiated in several countries using different approaches and methodology. We randomly assigned twelve premature infants(gestational age < 32 week) at high risk of requiring erythrocyte transfusion for anemia of prematurity with either subcutaneous recombinant human erythropoietin or a placebo. Treatment with rHuEPO was initiated at a dose of 100 units/kg day for 3 days a week. All patients were given supplemental oral iron therapy at a dose of 3 mg/kg per day, as tolerated and oral vitamin E at a dose of 25 units per day. Treated and control babies did not differ with respect to weight, hematocrit, overall mean reticulocyte count or rate of growth respectively. However, reticulocyte counts increased earlier in patients given rHuEPO. We conclude that rHuEPO administration is safe and feasible at the dose studied.

• Journal of Veterinary Clinics
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• v.18 no.2
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• pp.105-110
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• 2001

The water celery, Oenanthe Javanica DC., is a perennial herb which mostly grows on a damp ground or a streamside. The water celery is widely distributed in Korea, Japan, china, Taiwan, Indonesia, Malaysia and the other countries. From ancient times, the water celery has widely been used for food or medicine. In herbal medicine, the water celery has a medical actions which are defervescence, diuresis, jaundice, edema, hypotensor, haematic, angiostasis, sthenia, appetizer. Recently, studies on the water celery are briskly being conducted. For example, studies on the morphologic property and classifications of origin of the water celery, studies on the chemical constituents of the water celery which contains vitamins, proteins and amino acids, studies on the bio activities of the water celery which contains flavonoids. However, there is not studies of hematological changes in dogs administrated the water celery until now. Consicontly, this study was conducted to observed the hematological changes in dogs administrated excessive amount of the water celery crude juice for a long period. In this studies, all dogs showed significantly decrease of the PCV and Hb, and increase of the WBC, GSH and reticulocytes, but did not significantly change in the RBC, MCV, MCHC and Met-Hb.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2003.11a
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• pp.61-61
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• 2003

The ethanol extract of peony root (Paeonia Lactiflora Pall, Paeoniaceae) and its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. And protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were performed. The ethanol extract of peony root (PRE), gallic acid and methyl gallate were shown to possess the significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and were revealed the inhibitory effect of lipid peroxidation as expressed by malondialdehyde (MDA) formation. They were also found to strongly inhibit hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, assessed by single cell gel electrophoresis. Furthermore, oral administration of 50% PRE (50% ethanol extract), gallic acid and methyl gallate potently inhibited micronucleated reticulocyte (MNRET) formation of mouse peripheral blood induced by KBrO3 treatment in vivo. Therefore, PRE containing gallic acid and methyl gallate may be a useful natural antioxidant by scavenging free radicals, inhibition of lipid peroxidation and protecting oxidative DNA damage.

• YAKHAK HOEJI
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• v.54 no.6
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• pp.423-428
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• 2010

The aim of this study was to evaluate the in vivo effect of galangin and the 70% ethanolic extract of Alpinia officinarum (AO) toward $KBrO_3$-induced DNA and chromosomal damage in mice. Galangin and AO inhibited the formation of 8-hydroxy-2'-deoxyguanosine (8-OH2'dG) as an indicator of DNA oxidative damage in the liver cell. Galangin and AO showed the inhibitory effect on the formation of DNA single strand break in the splenocyte by single cell gel electrophoresis (SCGE) assay and also inhibited micronucleated reticulocyte (MNRET) formation of peripheral blood in tail blood of mice. Vit-E revealed antigenotoxic effects in DNA and chromosome levels, but galangin was more potent active compound compare to vit-E under our experimental conditions. The results suggest that the extract of Alpinia officinarum containing galangin can modify the oxidative DNA and chromosomal damage and may act as chemopreventive agent against oxidative stress in vivo.

• Pediatric Infection and Vaccine
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• v.5 no.2
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• pp.302-307
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• 1998

Hemolytic anemia due to cold agglutinin disease is a known complication of Mycoplasma pneumoniae infection but is rarely observed, particularly in children. A case of Mycoplasma pneumonia complicated with hemolytic anemia is presented. A 7 year-old girl was adimitted because of fever, cough, sputum and pale appearance. Chest X-ray showed pneumonic consolidation of Rt. upper lobe, lingular division. Laboratory studies disclosed the following values : Hb 5.3g/dL, Hct 11.1%, reticulocyte 2.9%, indirect Coombs test negative, direct Coombs test(monovalent) Anti-C3d positive, Anti-IgG negative, Anti-IgM negative, cold agglutinin titer 1 : 256, mycoplasma antibody titer 1 : 640, total bilirubin 1.0mg/dL. Initial PBS before wanning showed agglutination of red blood cells. The diagnosis of cold agglutinin hemolytic anemia complicating mycoplasma pneumonia was made. And treatment with roxithromycin, prednisolone and avoiding cold exposure was initiated, and complete recovery ensued. We report a case of cold agglutinin hemolytic anemia complicating mycoplasma pneumonia in children.

• Advances in Traditional Medicine
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• v.7 no.5
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• pp.466-476
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• 2008

We have developed the tablet formulation of Triticum aestivum (wheatgrass) and investigated clinically, its effects in patients suffering from b-thalassemia (major) at K. T. Children Hospital, Civil Hospital, Rajkot. The tablets (wheatgrass powder 250 mg.) were given 3 times in a day for 9 months. Blood samples were collected at the start, after 6 months and 9 months and analyzed for various biochemical and hematological parameters. Treatment with wheatgrass formulation for 9 months produced significant decrease in hemoglobin, total RBC, eosinophil and reticulocyte counts. The mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were significantly increased was significantly increased. The serum ferritin was also significantly decreased. There was no influence on serum magnesium, serum iron and Thiobarbituric acid reacting substances (TBARS). Our data indicate that treatment with wheatgrass on patients with $\beta$-thalassemia (major) may have beneficial effects in the form of a decrease in ineffective erythropoiesis, stimulation of hemoglobin synthesis in RBC, decrease in iron load and decrease in eosinophil count.

• The Korean Journal of Physiology
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• v.4 no.1
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• pp.55-57
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• 1970

Erythropoietic activity in anemic Plasma of chronic posthemorrhagic anemic men was studied in rats after subcutaneous injection of anemic plasma. Anemic plasma was obtained from blood donors who sold their blood once or twice a week for one or two years to blood bank. Hemoglobin concentrations of 8 blood donors ranged between 4.6 and 8.4 gm/100 ml. Pooled plasma was treated by acidification-boiling method and adjusted to pH 7.5 by adding 0.1 N NaOH. 7ml/kg and 15ml/kg of anemic plasma filtrate was injected to 2 groups of rats respectively for 7 and 8 days. Hemoglobin concentrations, red blood cell counts and reticulocyte counts were observed before and after injection of anemic plasma and no change was induced by the injection. Subsequently, it was concluded that there was no erythropoietin of high titer in the plasma of chronic post-hemorrhagic anemic men.

• Korean Journal of Veterinary Research
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• v.2 no.2
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• pp.5-13
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• 1962

The blood samples of 280 suckling pigs and of 300 adult pigs were studied in a hope to establish normal hemograms of swine as reared in Korean local conditions. The examination included total counts of erythrocytes, leukocytes differential count, hemoglobinometry, and reticulocyte count. The results of this study are summarized as following. 1. Higher erythrocyte count was observed in adult pigs as compared with the values reported by other workers. The normal erythrocyte value for malewas $8.4{\pm}0.9$ millions with approximately 0.6 million less for female. 2. Relatively higher hemoglobin concentration was measured in the piglets at birth, which slightly decreased in piglets of 2nd and 3rd weeks. The Hb concentration was highest in piglets of 5th week amounting to 15.8gm/100ml of blood, and it gradually decreased to the adult-level thereafter. 3. The average diameter of erythrocyte was $6.6{\pm}0.72{\mu}$ in suckling pigs and $6.3{\pm}0.11{\mu}$ in adult pigs. This shows that erythrocytes of piglets are larger than those of adult pigs and the mean diameter is also arger than reported by other investigators. 4. The lower mean percentages of eosinopils, basophils, monocytes were found both in the blood of suckling and adult pigs. 5. The other blood values were in general agreements with the values reported by other workers.

• YAKHAK HOEJI
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• v.49 no.3
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• pp.249-254
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• 2005

The ethanol extracts of the mixed vegetables (Bioactive Vegetables, BV) and the mixed fruits (Bioactive Fruits, BF) were evaluated for their in vivo antioxidant activities. Four weeks treatment of oral administration was performed to mice. A $KBrO_3$ as a potent oxidant was used to induce the oxidative stress for in vivo experiment. BV and BF were shown to possess the significant inhibitory effect of lipid peroxidation as measured by the level of malondialdehyde (MDA) formation although the potencies were not higher than those of well-known antioxidants such as vitamin C, trolox and quercetin. Furthermore, BV and BF inhibited DNA damage assessed by single cell gel electrophoresis (comet assay) and reduced the micronucleated reticulocyte (MNRET) formation of peripheral blood. Antioxidants tested also revealed potent inhibitory activities higher than BV and BF. These antigenotoxic activity profiles were similar to that of abovementioned inhibition of lipid peroxidation. Therefore, BV and BF having mild antioxidant activity as functional food candidates may be useful natural antioxidants by the inhibiting of lipid peroxidation and the protecting oxidative DNA and chromosomal damage.

• Journal of Applied Biological Chemistry
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• v.43 no.3
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• pp.125-130
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• 2000

An antiviral protein (CAP30) with ribosome-inactivating activity was purified from the leaves of Chenopodium album L. through ammonium sulfate precipitation and column chromatography using S-Sepharose, Blue-Sepharose, FPLC Suprose12 HR, and FPLC Mono-S. The molecular wight of CAP30 was estimated to be 30kD. CAP30 was thermostable, maintaing its activity even after incubation at $70^{\circ}C$ for 30 min, and was stable in the pH range of 6 to 9. In a cell-free in vitro translation system using rabbit reticulocyte lysate, protein synthesis was inhibited by the addition of CAP30 with an $IC_{50}$ of 2.26pM. The comparison of N-terminal amino acid sequences of this protein with known ribosome-inactivating proteins (RIPs) revealed that it had some sequence homology with PAP-S and PAP-R from pokeweed (Phytolacca americana)and dodecandrin from P. dodecandra, but had no sequence homology with RIPs from other plants belonging to different orders. The mosaic symptoms on tobacco leaves caused by cucumber mosaic virus infection was completely inhibited by 100 ng/ml of the pure CAP30 protein.