• The Journal of the Korean Society for Microbiology
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• v.35 no.4
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• pp.289-297
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• 2000

Background: Staphylococcus aureus (s. aureus) and Escherichia coli (E. coli) are major pathogens in community and hospital. And they sometimes cause the outbreak in hospital in the immunocompromised patients. Pulsed-field gel electrophoresis (PFGE) has been regarded as a standard method for genotyping in epidemiologic studies, but it is laborious and time-consuming. Infrequent restriction site-polymerase chain reaction (IRS-PCR), a new genotyping methods, was performed to compare the applicability with PFGE. Methods: We performed PFGE and IRS-PCR on S. aurues (n=120) and E. coli (n=117) which were collected clinically in 4 different hospitals. We assessed each method in terms of discriminatory power, quality, and efficiency. Results: In E. coli, the discriminatory power of IRS-PCR was $46.7{\sim}86.7%$, and that of PFGE was $88.9{\sim}96.7%$ according to hospital. But in S. aurues, the discriminatory power of IRS-PCR was $20{\sim}56.7%$, and that of PFGE was $40{\sim}90%$ according to hospital. The typablity and reproducibility of IRS-PCR were 100% of each. PFGE needed four days to complete the procedure, but IRS-PCR could be performed within one day, IRS-PCR showed better resolution than PFGE. Conclusion: In case of gram negative bacteria (like E. coli), IRS-PCR could be a reliable alternative for epidemiologic typing due to better efficiency and comparable discriminatory power. But in the case of gram positive bacteria (like S. aureus), IRS-PCR does not seem to be suitable for the strain-to-strain differentiation. More trials and changes of restriction enzymes or primers could reveal the efficacy of IRS-PCR in the field of molecular typing.

• Korean Journal of Veterinary Research
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• v.49 no.1
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• pp.45-57
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• 2009

The molecular genetic characterization of Aujeszky's disease virus (ADV) Yangsan strain (ADVYS), a Korean isolate, was investigated by analyzing the electrophoresis patterns and the physical maps of the viral DNA digested with various endonucleases. To establish DNA library for ADV-YS, twelve major BamHI restricted segments were cloned. Each location of the segments in the ADV genome was determined by sequence comparison with the sequences reported in Genbank and those sequences of the both termini of the segments. Physical maps were constructed based on the electrophoresis patterns of the digested viral DNA by restriction endonuclease and the results of Southern blot analyses with various DIG labeled probes originated from those of enzyme restricted segments of virulent (Shope) and avirulent (Bartha) strain. Comparing ADV-YS with a standard strain of Kaplan in the maps of restriction enzymes, following major respects were identified: (i) disappearance of BamHI restriction site between the first and second BamHI segments, (ii) creation of the BamHI restriction site in the fifth segment, and (iii) generation of the BglII site in the unique short (US) region. The genome of ADV-YS also contains a type 2 herpesvirus DNA molecule (in which the US region only inverts itself relative to the unique longregion) like all other ADV strains except Norden strain(type3), analyzed up to date. The size of the ADV genome estimated from the sizes of the restriction enzyme fragments, was approximately 145.3 kb (BamHI) or 145.4 kb (BglII). BamHI enzyme cleavage patterns were compared among the five Korean ADV isolates: Yangsan, Yongin, Dangjin, Jincheon and Iksan strains. Difference either in the number or in the size of the DNA fragments, suspected regions of termini of IR and TR, could be detected among all five strains.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2010.04a
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• pp.139-142
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• 2010

ENC(Electronic Navigational Chart) is an electronic chart that digitize nautical chart including all kinds of information of nautical chart. Mariners have difficulty reading information because of clutter problem if display scale of ECDIS is lower than compilation scale of ENCs. IHO made SCAMIN improvement model included in S-65 Standard to settle this ENC clutter problem. This SCAMIN model includes both simple restriction that should safety some requirement and share geographic information. and hard restriction that should overlap with area object such as depth area, land area, dredge area. It also apply SCAMIN step of sounding value according to importance of safety navigation. In this paper, we analyzed the restrictions for SCAMIN model of S-65 Standard, developed algorithm and application to apply theses restrictions reasonably and mechanically. Also we applied this algorithm and application to Korea ENCs and evaluated the result of performance.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.11
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• pp.2403-2412
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• 2010

ENC(Electronic Navigational Chart) is an electronic chart including all kinds of information of nautical chart. Mariners have difficulty reading information because of clutter problem if display scale of ECDIS is lower than compilation scale of ENCs. IRO made SCAMIN improvement model included in S-65 Standard to settle this ENC clutter problem. This SCAMIN model includes both simple restriction that should satisfy some requirement and share geographic information, and hard restriction that should overlap with area object such as depth area, land area, dredge area. It also apply SCAMIN step of sounding value according to importance of safety navigation. In this paper, we analyzed the restrictions for SCAMIN model of S-65 Standard, developed algorithm and application to apply theses restrictions reasonably and mechanically. Also we applied this algorithm and application to Korea ENCs and evaluated the result of performance.

• Korean Journal of Logic
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• v.21 no.1
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• pp.59-96
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• 2018

${\bot}$It is often said that in a purely formal perspective, intuitionistic logic has no obvious advantage to deal with the liar-type paradoxes. In this paper, we will argue that the standard intuitionistic natural deduction systems are vulnerable to the liar-type paradoxes in the sense that the acceptance of the liar-type sentences results in inference to absurdity (${\perp}$). The result shows that the restriction of the Double Negation Elimination (DNE) fails to block the inference to ${\perp}$. It is, however, not the problem of the intuitionistic approaches to the liar-type paradoxes but the lack of expressive power of the standard intuitionistic natural deduction system. We introduce a meta-level negation, ⊬$_s$, for a given system S and a meta-level absurdity, ⋏, to the intuitionistic system. We shall show that in the system, the inference to ${\perp}$ is not given without the assumption that the system is complete. Moreover, we consider the Double Meta-Level Negation Elimination rules (DMNE) which implicitly assume the completeness of the system. Then, the restriction of DMNE can rule out the inference to ${\perp}$.

• Journal of the Korean Society of Hazard Mitigation
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• v.6 no.2 s.21
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• pp.37-44
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• 2006

Any vehicle and equipment whose total weight is more than 40ton and its axle weight is 10ton or above is banned to cross any bridge in our country under section 54 in the Highway law. This restriction results from the accumulation and application of safety factors about which there is type specification in the "standard design vehicle". And in "standard design vehicle", Vehicle load to bridge is assumed concentrating one. Based on this restriction, there is an issue that military tank which has a total weight of 51ton (63ton in case of the US tank) can not cross any bridge. However, many research and practical examples concerned manifest that it is possible for military tanks to cross these bridges. The reasons of this issue in the current Highway law's provisions are analyzed in this paper. Correspondingly, feasibility of military tanks passing these bridges are discussed here. At last, considering economical efficiency and practicability for military, several suggestions and improving measures are put forward. This research has certain reality significance to guide bridge design considering the passage of military heavy vehicles.

• Journal of Advanced Navigation Technology
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• v.20 no.6
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• pp.580-587
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• 2016

In the purpose of suggesting the necessity and proper method of providing the standard reference material for acquiring the aircraft pilot license, this study provides the FAA and ROKAF case study, and positively analyzes the materials of 178 pilots and student pilots who acquired the aircraft pilot license. The result of case study shows that compared to the case of Korea which the restriction on the standard reference material providing exists, the FAA(Federal Aviation Administration) and ROKAF are currently providing the standard reference material. Additionally, the result of positive analysis indicates the fact that the necessity of standard reference material providing is well recognized and its importance also found to be remarkably high. These results represent that the standard reference material providing is necessary for training competent aircraft pilots. Also, this will mitigate the current shortage of aircraft pilots due to the rapid growth of air transportation industry, and this will play an important role in preventing diverse aircraft-related accidents.

• Child Health Nursing Research
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• v.12 no.3
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• pp.405-416
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• 2006

Purpose: The purpose of this study was to explore health behavior and perception of therapeutic restrictions in chronically ill children and their parents in Korea. Method: Nine children with chronic disease and of six of their parents were interviewed using semi-structured a questionnaire. The data were analyzed using explorative content analysis. Results: Health behaviors related to therapeutic restrictions was classified into four domains, and the perceptions of therapeutic restrictions into two domains. The domains regarding compliance in health behavior with therapeutic restrictions included control-centered restrictions (maintaining food limitations, avoiding harmful environments, restriction on physical activity, restriction on social activity, restriction on learning activity), and everyday pursuit of balance(preference for healthy diet, maintaining a regular life style, maintaining a standard body weight, pursuing psychological well-being, family participation). Domains regarding perception of therapeutic restrictions included obstacles to growth and development (bridled life, opportunity deprivation, prevented from playing proper role), origin of conflict (tenacity, conflict, stressor, cover-up), task for normal life (doing proper duty), and everyday affairs (becoming ordinary, familiarity). Conclusion: This study will help to enhance understanding the behavior and perception of therapeutic restrictions by chronically ill children and their families and to establish educational programs and counseling for these children and their families.

• Korean Journal of Microbiology
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• v.24 no.1
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• pp.7-11
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• 1986

We screened lysates of the laboratory strains of pseudomonads utilizing hydrocarbon by agarose gel electrophoresis and cesium chloride-ethidium bromide equilibrium centrifugation, to find an intrinsic plasmid as a vector and to examine the relationship between the plasmid and hydrocarbon degradation. Only one strain from the examined strains, Pseudomonas putida KU190, contained a plasmid. We named the plasmid pKU41. The molecular size of pKU41 was determined as 41kb, using covalently closed circular forms of RP4 and pSY343 as standard size markers. The restriction sites of pKU41 for BamHI, BglII, EcoRI, HindIII, and SalI were 3, 1, 3, 6 and more than 13, respectively. With double or triple digestion, restriction map of pKU41 was constructed for BamHI, BglII and HindIII. For elucidation on the biological function of the plasmid, test was conducted on the ability of hydrocarbon utilization of the host strain but no apparent relationship was observed.

• Korean Institute of Interior Design Journal
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• v.20 no.6
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• pp.340-349
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• 2011

The Act of Disability Discrimination and Rights Restriction established in 2008 states that all services including cultural and artistic activities should be fair and easily accessible for both disabled and non-disabled. While the previous society focused on providing the basic necessaries of life to disabled, the modern society gradually tuming their attention to improving quality of the life of disabled, especially in culture and art related activities. The Act of Disability Discrimination and Rights Restriction also states that the services for cultural and artistic activities should be provided from 2010. However in reality, there are contradictions among the Disability Discrimination Act, the Convenience Improvement Act for the Disabled, the Aged, and the Pregnant Woman, and the laws related architecture. So they are having difficulties technically with applying these laws. First, this research contains the comparison of domestic and foreign acts related to the legitimate accommodation uses of cultural and arts facilities for the disabled. Second, this research also lists the facts of conflict between the laws of legitimate accommodation uses for the disabled and the Architecture related laws. Finally, several suggestions are stating for the complementarily improved architecture-related laws which were based on the standard of foreign countries for the disabled.