• Journal of Nutrition and Health
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• v.8 no.3
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• pp.31-37
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• 1975

The effect of dietary restriction was obserbed in Albino rats. Dietary restriction was at 2 levels: 10%, 20%, 30 males and same number of females of Albino rats, aged $30{\sim}40$ days were devided into following 6 groups, 5 rats each. 1 group; rice standard group 2 group; 10% restriction of rice standard 3 group; 20% restriction of rice standard 4 group; 65% rice+35% chinese cabbage group 5 group; 10% restriction of 65% rice+35% chinese cabbage 6 group; 20% restriction of 65% rice+35% chinese cabbage The rats were kept in individual cage and given 6 different diet for 7 weeks. The results of this study were elucidated as follow. 1. Final body weight was not obserbed any significant differences between non-restriction group and 10% restriction group, but 20% restriction group revealed the decrease of body weight. 2. According to dietary restriction, final organ weight was almost decreased in the restriction group of rice+chinese cabbage, and in rice standard group, there were no significants differences between none-restriction group and 10% restriction group, but organ weight was sharply decreased such as Liver, Heart, Kidney, Adrenal, Spleen in 20% restriction group. Sex organ weight of female was increased but weight of male was decreased in 20% restriction group of rice standard group. 3. The result of biochemical analysis such as nitrogen retention, serum cholesterol, glucose of urine, feces, and serum, and lipid of feces and liver, was not revealed any significant diffences, but in 20% restriction group, urinary glucose and serum glucose levels were somewhat decreased, and liver nitrogen level was increased. 4. Therefore there is no effect on albino rats by 10% restricted diet. It can be concluded that it is possible to restrict the diet within 10%.

• The Journal of the Korean Society for Microbiology
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• v.22 no.4
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• pp.463-471
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• 1987

The genomes of leptospiral field isolates from Korea belonging to serogroup Icterohaemorrhagiae (21 strains) and serogroup Canicola (1 strain) were analysed and compared by restriction enzyme analysis with EcoRI and HindIII as digesting enzymes. One isolate belonging to serogroup Canicola showed the same pattern as serovar portlandvere. All 21 isolates belonging to serogroup Icterohaemorrhagiae showed almost same patterns as Leptospira serovar lai from China, But with very slight differences 21 isolates could be classified into 8 subtypes and these grouping seems to reflect the differences in epidemiological niche. And also the geographical data consisted with the grouping into 8 subtypes. According to our results, we concluded that the restriction endonuclease analysis of chromosomal DNA will be an accurate and reliable method to compare and classify pathogenic leptospires.

• Journal of the Korean Society of Physical Medicine
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• v.14 no.4
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• pp.125-132
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• 2019

PURPOSE: The aim of this study was to help physical therapists involved in rehabilitation in the direction of clinical intervention in the treatment of stroke patients by understanding how an activity restriction of stroke patients affects depression and the quality of life associated with health. METHODS: This study assessed 404 stroke patients from the Korean National Health and Nutritional Examination Survey. The subjects were divided into two categories: activity restriction and non-activity restriction. The variables used in this study were depression diagnosed by a doctor and EQ-5D, which evaluates the quality of life. Logistic regression analysis was used to calculate the odds ratios associations of activity restriction with depression and the quality of life in stroke patients. RESULTS: The diagnosis of depression with an activity restriction was 11.2%, while 4.8% of those with non-activity restrictions were diagnosed with depression (p<.05), and the EQ-5D index of was .68±.03, .88±.01(p<.05). The odds ratio for depression with an activity restriction was 3.37 (95% CI, 1.49-7.63) compared to the non-activity restriction. A statistically significant difference in the odds ratio was observed for each item in the EQ-5D. CONCLUSION: Activity restriction in stroke patients increases the probability of depression, and reduces the quality of life significantly. The treatment for stroke patients should be approached, taking psychological factors into account. In addition, rehabilitation programs that can be performed systematically and continuously are deemed necessary.

• Journal of Convergence for Information Technology
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• v.11 no.3
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• pp.87-94
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• 2021

This study was conducted to identify the mental health and quality of life of cardiovascular disease patients by their activities restriction through the second analysis of the 7th KNHANES collected from 2016 to 2018. The subjects of this study were 521 adults with angina or myocardial infarction who had no missing variables among 24,269 subjects. The difference between mental health and quality of life according to the activity restriction was analyzed by Chi-square method and the effect of mental health and quality of life by activity restriction by Logistic regression method using the SAS 9.4 version. The results of the analysis showed that the stress level, the depression for the last two weeks and suicide thought level was high when there was activity restriction, which had a significant effect on mental health. Mobility, selfcare and usual activities were disrupted, and pain/inconvenience and anxiety/depression increased when there was activity restriction, which also showed a significant effect on quality of life. Therefore, it is suggested that the development and operation of effective physical activity promotion program to minimize activity restriction is necessary to improve mental health and quality of life of cardiovascular patients.

• Journal of Ginseng Research
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• v.14 no.2
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• pp.310-316
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• 1990

This study was focused on the characterization of mitochondrial DNA (mtDNA) for molecular 9enetical approach of energy Production related mechanism in Panax ginseng. The simple and efficient method of mtDNA isolation from ginseng has been developed by modification of recently advanced methods. This procedure can successfully apply to mtDNA isolation of several plants. mtDNA of etiolated shoot and one-year root were digested with restriction endonucleases, but that of 6-year root not. Any difference was not observed in the restriction endonuclease digestion patterns among the ginseng variants. Molecular size of ginseng mtDNA was estimated at least 159 kb by the restriction endonuclease fragment analysis. The 4.5 kb extra band at the lane of EcoRII treatment could be observed in restriction patterns digested with the methylation sensitive endonucleases, BstN I and EcoRII. For construction of mitochondrial genomic library of ginseng, mtDNA was partially digested with EcoRl, and packaged with EMBL4 phage vector. Genomic library was screened and purified for further research including restriction mapping of ginseng mtDNA, and cloning of the genes. The gene of ATP synthase A subunit was cloned from the purified EMBL4 library clone No. 16. Now, clone No. 16 is subcloned for structure gene sequence analysis.

• Journal of Korean society of Dental Hygiene
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• v.21 no.5
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• pp.629-637
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• 2021

Objectives: The purpose of this study was to investigate the degree of activity restriction and the number of remaining teeth of the elderly and to analyze the correlation. Methods: Statistical analysis of the collected data was analyzed using the SPSS window program 25.0 version (IBM) using a composite sample for the analysis of the 7th National Health and Nutrition Survey data, and a composite sample multiple logistic regression analysis was performed to confirm the effect of activity restrictions on the number of remaining teeth. Results: As a result of analyzing the effect of activity restriction on the number of remaining teeth, in the model that analyzed only activity restriction variables, the risk ratio of less than 20 remaining teeth was 1.61 times higher and the age-adjusted model, the risk ratio of less than 20 remaining teeth was 1.46 times higher (p<0.001). In the model that corrected all variables, the results were not statistically significant. Conclusions: In this study, the relationship between activity restriction and the number of remaining teeth could be confirmed, and in subsequent studies, more in-depth studies are considered to be needed using sub-variables that specifically reflect the reasons for activity restriction.

• Journal of Ecology and Environment
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• v.31 no.4
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• pp.309-316
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• 2008

To develop a monitoring method for soil microbial communities in rice paddy fields, we used terminal restriction fragment length polymorphism (T-RFLP) to compare soil bacterial community structure in rice paddy fields experiencing different management practices: organic practices, conventional practices without a winter barley rotation, and conventional practices with a winter barley rotation. Restriction fragment length profiles from soils farmed using organic practices showed very different patterns from those from conventional practices with and without barley rotation. In principal component analyses, restriction fragment profiles in organic practice samples were clearly separated from those in conventional practice samples, while principal component analysis did not show a clear separation for soils farmed using conventional practices with and without barley rotation. The cluster analysis showed that the bacterial species compositions of soils under organic practices were significantly different from those under conventional practices at the 95% level, but soils under conventional practice with and without barley rotation did not significantly differ. Although the loadings from principal component analyses and the Ribosomal DNA Project II databases suggested candidate species important for soils under organic farming practices, it was very difficult to get detailed bacterial species information from terminal restriction fragment length polymorphism. Rank-abundance diagrams and diversity indices showed that restriction fragment peaks under organic farming showed high Pielou's Evenness Index and the reciprocal of Simpson Index suggesting high bacterial diversity in organically farmed soils.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.5
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• pp.804-808
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• 1997

The genetic differentiation and characteristics of two oyster populations (Crassostrea gigas) in Korea were assessed based on the restriction fragment length polymorphisms (RFLP) analysis and the restriction patterns of subcloned mtDNA. The restriction fragments of twenty individuals in West Sea revealed an identical pattern, determined by 8 restriction enzymes. On the other hand, two haplotypes having variation at the HindIII site were shown in the specimens from South Sea; minor haplotypes (4 of 20) were similar to the results obtained from individuals in West Sea while major haplotypes were different from those in West Sea. It was suggested that oysters (C. gigas) of West Sea might have been introduced to South Sea. Each mitochondrial DNA from two oyster populations in Korea and from one in Japan was divided to three parts and subcloned into pUC19 to use in genetic studies effectively. Restriction map was constructed based on the cleavage pattern by multiple restriction enzymes.

• Journal of Microbiology and Biotechnology
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• v.19 no.4
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• pp.346-350
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• 2009

Although salt is known to influence the performance of nitrification significantly, it has not been well reported on how salt affects ammonia-oxidizing bacterial(AOB) community compositions and dynamics in wastewater treatment bioreactors. In this study, these questions were evaluated in a full-scale bioreactor treating saline wastewater. Clone library analysis for the ammonia monooxygenase subunit A gene revealed that AOB belonging to the Nitrosomonas europaea and the N. oligotropha lineages inhabited in the bioreactor. Terminal restriction fragment length polymorphism analysis for monthly samples demonstrated a fluctuation pattern among AOB populations, although AOB within the N. europaea lineage were dominant during the test period. Correlation analysis between patterns of terminal restriction fragments and environmental variables suggested that sodium, chloride, and sulfate were less important; rather, temperature was the most significant factor affecting the AOB community in the bioreactor.

• Journal of the Korean Society of Physical Medicine
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• v.10 no.1
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• pp.63-69
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• 2015

PURPOSE: Low-intensity exercise with transient restriction of blood flow to muscle could be an alternative rehabilitation method which avoids the problems associated with conventional high-intensity exercise. However, the mechanism of low-intensity exercise with transient restriction of blood flow is not clearly known. Thus, the purpose of this study was to investigate the mechanism of improvement of muscular function after low-intensity exercise with transient restriction of blood flow using H-reflex analysis. METHODS: Twenty one healthy young adults with no medical history of neurological or musculoskeletal disorder voluntarily participated in this study. The ${\alpha}$-motor neuron excitability of the triceps surae was assessed using the H-reflex. The amplitude of the M-wave and H-reflex were measured across three conditions: rest, after low-intensity exercise without restriction of blood flow and after low-intensity exercise with restriction of blood flow. The subjects performed low-intensity ankle plantar flexion exercise at their own pace for one minute without or with transient restriction of blood flow achieved by a sphygmomanometer cuff on popliteal fossa at a pressure of 120mm of mercury(120 mmHg). RESULTS: No significant changes of the excitability of the ${\alpha}$-motor neuron were obtained across three different conditions. CONCLUSION: This study found that low-intensity exercise with transient restriction of blood flow did not influence to ${\alpha}$-motor neuron excitability of the triceps surae. From the results, I could come to the conclusion that further study will be required.