• Molecules and Cells
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• v.37 no.12
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• pp.907-911
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• 2014

The function of reactive oxygen species (ROS) as second messengers in cell differentiation has been demonstrated only for a limited number of cell types. Here, we used a well-established protocol for BMP2-induced neuronal differentiation of neural crest stem cells (NCSCs) to examine the function of BMP2-induced ROS during the process. We first show that BMP2 indeed induces ROS generation in NCSCs and that blocking ROS generation by pretreatment of cells with diphenyleneiodonium (DPI) as NADPH oxidase (Nox) inhibitor inhibits neuronal differentiation. Among the ROS-generating Nox isozymes, only Nox4 was expressed at a detectable level in NCSCs. Nox4 appears to be critical for survival of NCSCs at least in vitro as down-regulation by RNA interference led to apoptotic response from NCSCs. Interestingly, development of neural crest-derived peripheral neural structures in Nox4-/- mouse appears to be grossly normal, although Nox4-/- embryos were born at a sub-Mendelian ratio and showed delayed over-all development. Specifically, cranial and dorsal root ganglia, derived from NCSCs, were clearly present in Nox4-/- embryo at embryonic days (E) 9.5 and 10.5. These results suggest that Nox4-mediated ROS generation likely plays important role in fate determination and differentiation of NCSCs, but other Nox isozymes play redundant function during embryogenesis.

• Molecules and Cells
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• v.37 no.12
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• pp.857-864
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• 2014

Astrocyte elevated gene-1 (AEG-1) is a recently discovered oncogene that has been reported to be highly expressed in various types of malignant tumors, including renal cell carcinoma. However, the precise role of AEG-1 in renal cancer cell proliferation and apoptosis has not been clarified. In this study, we transfected the renal cancer cell line Caki-1 with a plasmid expressing AEG-1 short hairpin RNA (shRNA) and obtained cell colonies with stable knockdown of AEG-1. We found that AEG-1 down-regulation inhibited cell proliferation and colony formation and arrested cell cycle progression at the sub-G1 and G0/G1 phase. Western blot analysis indicated that the expression of proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E were significantly reduced following AEG-1 down-regulation. In addition, AEG-1 knockdown led to the appearance of apoptotic bodies in renal cancer cells, and the ratio of apoptotic cells significantly increased. Expression of the antiapoptotic factor Bcl-2 was dramatically reduced, whereas the pro-apoptotic factors Bax, caspase-3 and poly (ADPribose) polymerase (PARP) were significantly activated. Finally, AEG-1 knockdown in Caki-1 cells remarkably suppressed cell proliferation and enhanced cell apoptosis in response to 5-fluorouracil (5-FU) treatment, suggesting that AEG-1 inhibition sensitizes Caki-1 cells to 5-FU. Taken together, our data suggest that AEG-1 plays an important role in renal cancer formation and development and may be a potential target for future gene therapy for renal cell carcinoma.

• Journal of the Korean Chemical Society
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• v.35 no.6
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• pp.659-666
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• 1991

A systematic analysis of 18 stimulants and narcotic analgesics containing nitrogen atom (s) in human urine by gas chromatography with nitrogen phosphorus detector (GC-NPD), is described. The urinary extract with diethyl ether at pH 8.5 showed good recoveries of the drugs and less interference peaks on GC chromatogram. Retention data were standardized by the calculation of relative retention times using diphenylamine as the internal standard. The relative standard deviations of retention times were less than 0.1% for the within-run analyses. The response factor (RRF) of a drug relative to the internal standard was calculated. RRF decreased with increasing number of nitrogen atoms. This technique can be adapted to various analytical toxicology problems.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.6
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• pp.279-283
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• 2012

In is paper, we design and implement the low power, high speed touch screen controller that calculates and outputs the coordinate of touch point on the touch screen of mobile devices. The system clock is 10HMz, the number of input channels is 21, standby current is $20{\mu}A$, dynamic range of input is 140pF~400pF and the response time is 0.1ms/frame. It contains the power management unit for low power, automatic impedance calibration unit in order to adapt to humidity, temperature and evaluation board, adjacent key and pattern interference suppression unit, serial interface unit of I2C and SPI. The function and performance is verified by using FPGA and $0.18{\mu}m$ CMOS standard process. The implemented touch screen is designed for using in the double layer ITO(Indium Thin Oxide) module with diamond pattern and single layer ITO module for cost-effective which are applied to mobile phone or smart remote controller.

• Journal of the Society of Naval Architects of Korea
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• v.52 no.6
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• pp.452-459
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• 2015

It is important to use bench test results in the design process of anti-rolling tanks. Traditional bench tester is usually designed to perform only roll motions about a fixed axis and relatively small so that the viscous effects may not be neglected. Novel bench tester which could adjust the motion center to realize the coupled motion of sway and roll has been devised and manufactured therefore, large scaled bench tester could be utilized for designing the passive anti-rolling tanks. The time history of the reference signal from the rotation sensor of the bench tester have been recorded and processed to determine the phase angle to derive the Response Amplitude Operator(RAO) of the stabilized ship. The breadth of ART tank model should be large up to 2 m to diminish viscous scale effect and the vertical position of the tank can be varied with the ship's center of motion. The periods and the amplitude of roll motion can be varied from 1.5 sec to 5 sec and up to ±20°, respectively. The components of the tester was expressed in three dimensional digital mockup (DMU) and assembled together in the CAD space. The final configuration of the bench tester has been determined by confirming the smooth operation of the moving parts without interference through the animation in CAD space. New analytic logic are introduced for the determination of hydrodynamic moment and phase difference due to fluid motion in ART and verified through the test. The developed bench tester is believed to be effective and accurate for the verification of stabilization effect of ART taking into the consideration of the sway effect in the design stage.

• Korean Journal of Optics and Photonics
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• v.17 no.1
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• pp.54-59
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• 2006

Biophotonic sensors based on polymer waveguide with Bragg reflection grating are demonstrated in this work. Waveguide Bragg reflectors were designed by using the effective index method and the transmission matrix method. The grating pattern was formed by exposing the laser interference pattern on a photoresist. On top of the inverted rib waveguide, the Bragg reflection grating was inscribed by the O2 plasma etching. In order to perform the bio-molecule detection experiment, a calixarene molecule was self-assembled on top of thin Au film deposited on the waveguide Bragg reflector. To measure the response of the sensor, several PBS solutions with different concentrations of potassium ion from 1 pM to $100\;{\mu}M$ were dropped on the sensor surface. The shift of Bragg reflection wavelength was observed from the fabricated sensor device, which was proportional to the concentration of potassium ion ranging from 1 pM to 108 pM.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.23 no.10
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• pp.1166-1172
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• 2012

In this paper, by using a commercial EM simulator, we could obtain the array manifold which are phase responses of an array antenna for the incident plane wave and then verified the effectiveness of methodology after comparing with the measurement. The result shows that the array manifold can be calculated including not only the phase response of the ideal point sources but also the influences of the mutual coupling between antennas and the installed platform. Also it can exclude the interference of strong broadcasting signal and the disturbance of the multipath in the calibration process. Finally, to predict the performances of direction finding systems, a novel method using both the EM simulation-based receiving signal and the sparsely sampled array manifold with the parabolic estimation is proposed. This method can be utilized in the various fields of direction-finding since it shows the superior predictive performance even in low SNR conditions.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.28 no.4
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• pp.298-308
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• 2017

This paper presents a Interleaved Buck Converter(IBC) system with Random PWM to reduce electromagnetic noise by harmonics. Swithced mode power supply generally controlled by high switching frequency have a electromagnetic interference(EMI) issue due to the high-voltage/high-current switching to regulate the voltage in buck converter. To solve the problem. we present a novel IBC system with PRBS. IBC system has two active switches with 180 phase difference that controll the cicuit with two PWM signal. IBC system may be disadventageous for the cost due to the addtion of one set of switch, but it has adventages of power distribution, current ripple cancellation, fast transient response, and passive component size reduction. To verify the validity of study, simulation program has been bulit using PSIM and the experimental results of IBC system using RPWM was compared with the conventinal PWM and randomized PWM.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.5
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• pp.921-927
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• 2006

The current DGPS technique is many problems that is permission of radio station using RF Wireless Modem, that is influence of geographic obstacle using radio wave, that is frequency interference, that is finiteness of frequency resources. In this paper, we are solved many elements, IM(Interface Module) replaces RF Wireless Modem, we suggest transmission technique of correction message using mobile phone, we researched Interface Module development which is linkage of DGPS receiver and mobile phone. IM can transmit correction message passing RS-232 port and modem communication control. IM of base station is initialized RS-232 port and modem to move station for correction message transmission, IM waited response mode. IM of move station is initialized RS-232 port and modem, IM requests hand shaking to base station, completed connection establishment. Users are worked Differential surveying using receiving correction message between mobile phones.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• v.2B no.3
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• pp.115-124
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• 2002

This paper presents an active auxiliary edge-resonant DC link snubber with a ringing surge damper and a three-phase voltage source type zero voltage soft-switching inverter with the resonat snubber treated here for the AC servo motor driver applications. The operation of the active auxiliary edge-resonant DC link snubber circuit with PWM voltage is described, together with the practical design method to select its circuit parameters. The three-phase voltage source type soft-switching inverter with a single edge-resonant DC link snubber treated here is evaluated and discussed for the small-scale permanent magnet (PM) type-AC servo motor driver from an experimental point of view. In addition to these, the AC motor stator current and its motor speed response for the proposed three-phase soft-switching inverter employing Intelligent Power Module(IPM) based on IGBTS are compared with those of the conventional three-phase hard-switching inverter using IPM. The practical effectiveness of the three-phase soft-switching inverter-fed permanent magnet type AC motor speed tracking servo driver is proven on the basis of the common mode current in a novel type three-phase soft-switching inverter-fed AC motor side and the conductive noise on the mains terminal interface voltage as compared with those of the conventional three-phase hard-switching inverter-fed permanent magnet type AC servo motor driver for the speed tracking applications.