• 대한한방내과학회지
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• 제31권3호
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• pp.488-499
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• 2010

Objective : This study was designed to investigate the correlation between Qi movement stagnation condition, stress level and xerostomia in halitosis patients. Methods : Ninety-nine halitosis patients visiting the Halitosis Clinic in the hospital of Oriental medicine, Kyung Hee University from January, 2010 to May, 2010 were recruited. The subjects were assessed on their stress levels(based on the Stress Response Inventory), xerostomia symptoms(based on the 8-item Visual Analogue Scale xerostomia questionnaire), and Qi movement stagnation(氣鬱) condition(one of the subcategories in the Qi, blood and water pattern identification(氣血水辨證)). Salivary functions were evaluated using the unstimulated whole salivary flow rate measurements. Results : Compared to the non-Qi movement stagnation group(Qi movement stagnation pattern identification score < 30), the confirmed Qi movement stagnation group(Qi movement stagnation pattern identification score $\geqq$ 30) showed higher levels of xerostomia and stress. In the regression analysis, the Qi movement stagnation condition and stress levels showed a significant correlation. Furthermore, the Qi movement stagnation condition and stress levels each displayed significant correlations with xerostomia. Conclusions : The results of this study suggest that stress and Qi movement stagnation condition contribute to xerostomia symptoms in halitosis patients. Considering the correlation found between the Qi movement stagnation and stress, this study suggests a novel methodology in treating xerostomia, halitosis, and other stress-related disorders through the Qi movement stagnation-related approach.

• 한국항공우주학회지
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• 제42권8호
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• pp.692-700
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• 2014

본 논문에서는 미소진동 영향성 예측을 위한 인공위성 유한요소모델 보정에 관해 기술한다. 최근 지구 저궤도에 발사되는 상업용 지구관측위성의 경우, 수요자의 요구로 인해 주어진 시간 안에 다양한 지역의 많은 영상을 공급해야 한다. 이를 구현하기 위해 고용량휠, 다축 김발을 탑재한 안테나 등 다양한 구동기를 사용한다. 그러나 이러한 구동기는 작동 시 미소진동을 유발할 수 있으며, 이러한 미소진동은 매우 작기는 하지만 지구관측 탑재체를 가진하여 영상품질을 저감시킬 수 있다. 이러한 미소진동에 의한 영향성을 살펴보기 위해서 유한요소모델과 미소진동원 시험결과를 결합한 연성해석을 실시하며, 해석에 앞서 유한요소모델 보정을 실시한다. 보정 전후의 주파수 차이, 모드형상 상관관계, 주파수응답함수 상관관계를 비교하여 개선된 유한요소모델의 품질을 평가하였다.

• 해양환경안전학회지
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• 제17권1호
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• pp.1-6
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• 2011

인간이 환경을 보전 복원하고자 하는 목적은 자연이 인간에게 제공하는 환경서비스를 지속적으로 공급받기 위함이다. 이러한 측면에서 유류오염에 대응하고 복원하는 근본적인 목적은 인간의 생활 및 생산환경의 지속성을 유지하기 위한 것이라 할 수 있다. 즉, 유류유출의 사회경제적 피해영향 및 복원정도 모니터링은 손상된 자연환경의 기능 회복에 중요한 요소이다. 하지만 사회경제적 영향평가는 직간접적 관련자들의 이해와 직결되어 있어 적절한 조사 및 평가 방법을 선택하는 것이 어렵고 결과를 해석하는 것도 어렵다. 본 연구에서는 허베이 스피리트호 유류유출 사고를 대상으로 생활 및 생산환경 피해 모니터링의 주요 지표인 수산, 관광부문의 영향평가를 위해 기존의 문헌 및 통계자료조사 외에 지역주민 체감조사를 병행하였다. 조사 결과, 복원의 수준은 40~50 % 수준이고, 복원정도는 둔화 혹은 감소하고 있는 것으로 나타났으며, 체감조사결과가 문헌 및 통계자료 조사결과 보다 2~10 점 정도 낮은 것으로 평가되었다. 결론적으로 기존의 사회경제적 영향평가를 위해 기본적으로 수행하여 왔던 문헌 및 통계자료를 통한 분석이 피해영향 복원의 지표로 삼기에는 한계가 있음을 보여주었고, 실제 피해지역 주민들이 체감하는 영향과 복원정도에 대한 분석의 필요성을 제기하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제8권6호
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• pp.339-344
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• 2004

Recent data have shown the importance of oxidative stresses in the pathogenesis of inflammatory bowel disease, crohn's disease and ulcerative colitis. $H_2O_2$, reactive oxygen species (ROS) donor, has been reported to act as a signaling molecule involved in a variety of cellular functions such as apo/ptosis and proliferation. In the present study, we investigated viability of cultured ileal smooth muscle cells (ISMC) after stimulation with $H_2O_2$. Trypan blue method revealed that the cell viability of ISMC treated with 1 mM $H_2O_2$ was not different from that of controls at up to 2 h time point, while treatment of ISMC with 1 mM $H_2O_2$ for 48 h finally induced significant decrease in the cell viability. Therefore, we evaluated whether $H_2O_2$ was capable of ERKs activation in ISMC for the short-term exposure and examined whether tyrosine kinase was involved in the process of ERK activation by $H_2O_2$ in ISMC. We also investigated the effects of $H_2O_2$ on activation of SAPK/JNK and p38 MAP kinase in ISMC. Thus, ISMC were cultured and exposed to $H_2O_2$, and western blot analysis was performed with phosphospecific MAP kinase antibodies. Robust activation of ERK occurred within 30 min of 1 mM $H_2O_2$ treatment. $H_2O_2-induced$ ERK activation was attenuated by a tyrosine kinase inhibitor, genistein, indicating that tyrosine kinase was probably involved in the ERK activation by $H_2O_2$. $H_2O_2$ was a moderate activator of SAPK/JNK, while p38 MAP kinase was not activated by $H_2O_2$. We suggest that ERK activation induced by short-term $H_2O_2$ treatment plays a critical role in cellular protection in the early stage of response to oxidative stress. The present study suggests the necessity of identification of MAPK signaling pathways affected by ROS, since it could ultimately elucidate cellular consequences involved in initiation and perpetuation of intestinal tissue damage in the diseases such as crohn's disease and ulcerative colitis, resulted from excessive ROS.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제27권1호
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• pp.16-23
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• 2005

It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.

• 한국환경과학회지
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• 제27권5호
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• pp.291-297
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• 2018

Vertical farming systems offer many advantages in urban spaces. They have also been proposed as an engineering solution to increase the productivity per unit area of cultivated land by extending crop production in the vertical dimension. However, soil water retention is a major constraint affecting the plant environment. This study analyzed the effects of growth environment of Tropaeolum majus and Fragaria spp., on the vertical farming system, by using four different types of cover material types including sphagnum moss (Control), a shading net (S.N.), multi-layered fabric (M.L.F.), and non-woven fabric (N.W.F.). The volumetric soil moisture contents and plant characteristics were investigated from May to September 2014. Plant materials were individually cultivated in hanging baskets measuring $30{\times}17{\times}17cm$, filled with a mixture of soil and perlite, and placed at 1.5m height. Each treatment was performed in quadruplicate and consisted of five plants, amounting to a total of 20 plants. The analysis indicated that different covers were associated with multiple functions and soil water retention improvements may have a positive impact on the vertical farming system. The difference in soil water retention increased in the following order: M.L.F. > Control > N.W.F. > S.N.. Furthermore, the differences in plant height and survival rate increased in the following order: M.L.F. > Control > N.W.F. > S.N. Therefore, M.L.F yielded satisfactory good response for the vertical farming system of cover materials. Our results clearly demonstrate that vertical spaces represent an attractive alternative to urban farming and suggest that further increases in yield may be achieved via different cover materials in vertical farming using hanging baskets.

• 비파괴검사학회지
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• 제33권5호
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• pp.465-471
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• 2013

원전 증기발생기는 원자로 냉각재 계통에서 발생한 열에너지를 터빈 계통의 주급수에 전달하여 터빈을 회전시키기 위한 증기를 생산하는 일종의 열교환기이다. 증기발생기 전열관의 손상은 증기발생기의 구조적 및 누설 건전성 유지 능력을 저해시키기 때문에 주기적으로 와전류검사를 수행하여 전열관의 건전성을 평가한다. 증기발생기 전열관의 건전성 평가는 보통 원자로 연료 재장전 기간 중에 수행된다. 현재 국내 증기발생기 전열관에 적용되는 와전류검사는 KEPIC 및 ASME 코드 요건에 따라 수행되며, 와전류검사 수행에 필요한 검사 시스템은 와전류검사 장치와 수집된 신호를 평가하기 위한 평가 프로그램으로 구성된다. 검사에 적용되는 와전류검사 시스템을 구성하는 핵심기기인 와전류검사 장치는 ASME 및 KEPIC 코드에서 총 고조파 왜곡율, 입출력 임피던스, 증폭기 직선성 및 안정성, 위상 직선성, 대역폭 및 복조필터 응답, 디지털 변환, 채널 간섭 등과 같은 전기적 특성을 측정하도록 규정하고 있다. 이에 따라 본 논문에서는 국내 최초로 개발한 원전 증기발생기 와전류검사 장치의 전기적 특성 측정을 위한 ASME 및 KEPIC 코드 요건을 설명하고, 이 요건에 따른 증기발생기 와전류검사 장치의 전기적 특성의 측정 결과를 제시하였다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2007년도 International Meeting of the Microbiological Society of Korea
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• pp.120-122
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• 2007

All living organisms use numerous signal-transduction pathways to sense and respond to their environments and thereby survive and proliferate in a range of biological niches. Molecular dissection of these signalling networks has increased our understanding of these communication processes and provides a platform for therapeutic intervention when these pathways malfunction in disease states, including infection. Owing to the expanding availability of sequenced genomes, a wealth of genetic and molecular tools and the conservation of signalling networks, members of the fungal kingdom serve as excellent model systems for more complex, multicellular organisms. Here, we employed Cryptococcus neoformans as a model system to understand how fungal-signalling circuits operate at the molecular level to sense and respond to a plethora of environmental stresses, including osmoticshock, UV, high temperature, oxidative stress and toxic drugs/metabolites. The stress-activated p38/Hog1 MAPK pathway is structurally conserved in many organisms as diverse as yeast and mammals, but its regulation is uniquely specialized in a majority of clinical Cryptococcus neoformans serotype A and D strains to control differentiation and virulence factor regulation. C. neoformans Hog1 MAPK is controlled by Pbs2 MAPK kinase (MAPKK). The Pbs2-Hog1 MAPK cascade is controlled by the fungal "two-component" system that is composed of a response regulator, Ssk1, and multiple sensor kinases, including two-component.like (Tco) 1 and Tco2. Tco1 and Tco2 play shared and distinct roles in stress responses and drug sensitivity through the Hog1 MAPK system. Furthermore, each sensor kinase mediates unique cellular functions for virulence and morphological differentiation. We also identified and characterized the Ssk2 MAPKKK upstream of the MAPKK Pbs2 and the MAPK Hog1 in C. neoformans. The SSK2 gene was identified as a potential component responsible for differential Hog1 regulation between the serotype D sibling f1 strains B3501 and B3502 through comparative analysis of their meiotic map with the meiotic segregation of Hog1-dependent sensitivity to the fungicide fludioxonil. Ssk2 is the only polymorphic component in the Hog1 MAPK module, including two coding sequence changes between the SSK2 alleles in B3501 and B3502 strains. To further support this finding, the SSK2 allele exchange completely swapped Hog1-related phenotypes between B3501 and B3502 strains. In the serotype A strain H99, disruption of the SSK2 gene dramatically enhanced capsule biosynthesis and mating efficiency, similar to pbs2 and hog1 mutations. Furthermore, ssk2, pbs2, and hog1 mutants are all hypersensitive to a variety of stresses and completely resistant to fludioxonil. Taken together, these findings indicate that Ssk2 is the critical interface protein connecting the two-component system and the Pbs2-Hog1 pathway in C. neoformans.

• 한국해안해양공학회지
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• 제19권3호
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• pp.213-221
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• 2007

전지구적으로 지구 온난화 문제가 대두되고 있는 현 상황에서, 기온변화에 수반되는 수온변화 반응을 파악하기 위하여 해양수산부에서 제공하는 마산만 연안해역의 기온 및 수온 관측자료를 이용하여 기온과 수온의 관계를 분석하였다. 수온과 기온의 무차원 자기상관함수와 교차상관함수 변화를 분석한 결과, 지체시간 10일 이내에서는 상관계수가 0.9 이상으로 매우 크게 나타났으며, 지점별로 수온의 상관계수보다 기온의 상관계수가 크게 나타나는 것으로 파악되었다. 마산만 기온 및 수온자료를 수온상승기, 수온하강기로 구분하여 분석한 결과, 수온상승기에는 MA1 지점, MA2 지점의 기울기가 각각 0.829, 0.774로 나타났으며, 수온하강기에는 MA1 지점, MA2 지점의 기울기가 각각 1.385, 1.444로 기온상승기에 비하여 기온하강기의 기울기가 약 1.75배 정도 크게 나타나고 있으며, 명확한 이력현상으로 파악되었다. 따라서, 마산만 연안해역의 기온-수온 상관관계를 보다 정확하게 결정하기 위해서는 기온-수온의 계절적인 이력현상을 포함하여야 하며, 이 경우 기온을 이용한 수온 추정결과의 정확도가 향상되는 것으로 파악되었다.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2214-2223
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• 2016

Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.