• Animal Bioscience
• /
• v.34 no.1
• /
• pp.134-142
• /
• 2021

Objective: To understand the athletic characteristics of Thoroughbreds, high-throughput analysis has been conducted using horse muscle tissue. However, an in vitro system has been lacking for studying and validating genes from in silico data. The aim of this study is to validate genes from differentially expressed genes (DEGs) of our previous RNA-sequencing data in vitro. Also, we investigated the effects of exercise-induced stress including heat, oxidative, hypoxic and cortisol stress on horse skeletal muscle derived cells with the top six upregulated genes of DEGs. Methods: Enriched pathway analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) tool with upregulated genes in horse skeletal muscle tissue after exercise. Among the candidates, the top six genes were analysed through geneMANIA to investigate gene networks. Muscle cells derived from neonatal horse skeletal tissue were maintained and subjected to exercise-related stressors. Transcriptional changes in the top six genes followed by stressors were investigated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The inflammation response pathway was the most commonly upregulated pathway after horse exercise. Under non-cytotoxic conditions of exercise-related stressors, the transcriptional response of the top six genes was different among types of stress. Oxidative stress yielded the most similar expression pattern to DEGs. Conclusion: Our results indicate that transcriptional change after horse exercise in skeletal muscle tissue strongly relates to stress response. The qRT-PCR results showed that stressors contribute differently to the transcriptional regulation. These results would be valuable information to understand horse exercise in the stress aspect.

• Fisheries and Aquatic Sciences
• /
• v.25 no.11
• /
• pp.559-571
• /
• 2022

Galectins, a family of ß-galactoside-binding lectins, have emerged as soluble mediators in infected cells and pattern recognition receptors (PRRs) responsible for evoking and regulating innate immunity. The present study aimed to evaluate the role of galectin-1 in the host immune response of redlip mullet (Liza haematocheilia). We established a cDNA database for redlip mullet, and the cDNA sequence of galectin-1 (LhGal-1) was characterized. In silico analysis was performed, and the spatial and temporal expression patterns in gills and blood in response to lipopolysaccharide polyinosinic:polycytidylic acid, and Lactococcus garvieae were estimated via quantitative real-time PCR. Functional assays were conducted using recombinant protein to investigate carbohydrate binding, bacterial binding, and bacterial agglutination activity. LhGal-1 was composed of 135 amino acids. Conserved motifs (H-NPR, -N- and -W-E-R) within the carbohydrate recognition domain were found in LhGal-1. The tissue distribution revealed that the healthy stomach expressed high levels of LhGal-1. The temporal monitoring of LhGal-1 mRNA expression in the gill and blood showed its significant upregulation in response to immune challenges with different stimulants. rLhGal-1 exhibited binding activity in response to carbohydrates and bacteria. Moreover, the agglutination of rLhGal-1 against Escherichia coli was observed. Collectively, our findings suggest that LhGal-1 may function as a PRR in redlip mullet. Furthermore, LhGal-1 can be considered a significant gene to play a protective role in redlip mullet immune system.

• The KIPS Transactions:PartD
• /
• v.10D no.4
• /
• pp.591-602
• /
• 2003

Delay and discontinuance phenomenon of service are cause by sudden increase of the network communication amount and the quantity consumed of resources when Internet users are driven excessively to a conventional single large database sewer. To solve these problems, spatial database cluster consisted of several single nodes on high-speed network to offer high-performance is risen. But, research about spatial join operation that can reduce the performance of whole system in case process at single node is not achieved. So, in this paper, we propose efficient parallel spatial join processing method in a spatial database cluster system that uses data partitions and replications method that considers the characteristics of space data. Since proposed method does not need the creation step and the assignment step of tasks, and does not occur additional message transmission between cluster nodes that appear in existent parallel spatial join method, it shows performance improvement of 23% than the conventional parallel R-tree spatial join for a shared-nothing architecture about expensive spatial join queries. Also, It can minimize the response time to user because it removes redundant refinement operation at each cluster node.

• Journal of Internet Computing and Services
• /
• v.5 no.1
• /
• pp.67-84
• /
• 2004

Real-time database systems (RTDBS) are database systems whose transactions are associated with timing constraints such as deadlines. Therefore transaction needs to be completed by a certain deadline. Besides meeting timing constraints, a RTDBS needs to observe data consistency constraints as well. That is to say, unlike a conventional database system, whose main objective is to provide fast average response time, RTDBS may be evaluated based on how often transactions miss their deadline, the average lateness or tardiness of late transactions, the cost incurred in transactions missing their deadlines. Therefore, in RTDBS, transactions should be scheduled according to their criticalness and tightness of their deadlines, even If this means sacrificing fairness and system throughput, And It always must guarantee preceding process of the transaction with the higher priority. In this paper, we propose an efficient real-time scheduling algorithm (Multi-level EFDF) that alleviates problems of the existing real-time scheduling algorithms, a real-time concurrency control algorithm(2PL-FT) for firm and soft real-time transactions. And we compare the proposed 2PL F[ with AVCC in terms of the restarting ratio and the deadline missing ratio of transactions. We show through experiments that our algorithms achieve good performance over the other existing methods proposed earlier.

• The KIPS Transactions:PartD
• /
• v.10D no.6
• /
• pp.935-948
• /
• 2003

An online scaling method adds new nodes to the shared-nothing database cluster and makes tables be reorganized while the system is running. The objective is to share the workload with many nodes and increase the capacity of cluster systems. The existing online scaling method, however, has two problems. One is the degradation of response time and transactions throughput due to the additional overheads of data transfer and replica's condidtency. The other is and inefficient recovery mechanism in which the overall scaling transaction is aborted by a fault. These problems deteriorate the availability of shared-nothing database cluster. To avoid the additional overheads throughout the scaling period, our scalingmethod consists of twophases : a parallel data transfer phase and a combination phase. The parallel data transferred datausing reduces the size of data transfer by dividing the data into the number of replicas. The combination phase combines the transferred datausing resources of spare nodes. Also, our method reduces the possibility of failure throughout the scaling period and improves the availability of the database cluster.

• KIPS Transactions on Computer and Communication Systems
• /
• v.8 no.6
• /
• pp.127-138
• /
• 2019

The data response speed is a critical issue of cloud services because it directly related to the user experience. As such, the in-memory database is widely adopted in many cloud-based applications for achieving fast data response. However, the current implementation of the in-memory database is mostly based on the linked list-based hash table which cannot guarantee the constant data response time. Thus, cuckoo hashing was introduced as an alternative solution, however, there is a disadvantage that only half of the allocated memory can be used for storing data. Subsequently, bucketized cuckoo hashing (BCH) improved the performance of cuckoo hashing in terms of memory efficiency but still cannot overcome the limitation that the insert overhead. In this paper, we propose a data management solution called Wall Cuckoo which aims to improve not only the insert performance but also lookup performance of BCH. The key idea of Wall Cuckoo is that separates the data among a bucket according to the different hash function be used. By doing so, the searching range among the bucket is narrowed down, thereby the amount of slot accesses required for the data lookup can be reduced. At the same time, the insert performance will be improved because the insert is following up the operation of the lookup. According to analysis, the expected value of slot access required for our Wall Cuckoo is less than that of BCH. We conducted experiments to show that Wall Cuckoo outperforms the BCH and Sorting Cuckoo in terms of the amount of slot access in lookup and insert operations and in different load factor (i.e., 10%-95%).

• Restorative Dentistry and Endodontics
• /
• v.39 no.2
• /
• pp.79-88
• /
• 2014

Appropriate use of local hemostatic agent is one of the important factors on the prognosis of endodontic microsurgery. However, most investigations to date focus on the hemostatic efficacy of the agents, whereas their biologic characteristics have not received enough attention. The purpose of this paper was to review the biologic response of local hemostatic agents, and to provide clinical guidelines on their use during endodontic microsurgery. Electronic database (PUBMED) was screened to search related studies from 1980 to 2013, and 8 clinical studies and 18 animal studies were identified. Among the materials used in these studies, most widely-investigated and used materials, epinephrine, ferric sulfate (FS) and calcium sulfate (CS), were thoroughly discussed. Influence of these materials on local tissue and systemic condition, such as inflammatory and foreign body reaction, local ischemia, dyspigmentation, delayed or enhanced bone and soft tissue healing, and potential cardiovascular complications were assessed. Additionally, biological property of their carrier materials, cotton pellet and absorbable collagen, were also discussed. Clinicians should be aware of the biologic properties of local hemostatic agents and their carrier materials, and should pay attention to the potential complications when using them in endodontic microsurgery.

• Journal of Acupuncture Research
• /
• v.19 no.3
• /
• pp.138-155
• /
• 2002

Objective : The purpose of this study is to work on the problems and on the direction of the study regarding the evidence-based study of Acupuncture. Methods : We reviewed the PubMed electronic database. Eight articles of systematic review without meta-analysis and six articles without meta-analysis were selected. Results : In the study of eight articles of systematic review without meta-analysis, there were two articles on effectiveness of acupuncture and one on non-effectiveness of acupuncture. Four articles were about the changes according to the quality of randomized controlled trials. Also, one article was about the side-effect of acupuncture. In the study of six articles of systematic review with meta-analysis, there were two articles on Odds Ratio, two on Relative Risk, one on number-needed-to-treat, and two on 18 predefined methodological criteria. Conclusion : As the quality of RCTs was high, positive response decreased and as the quality of RCTs was low, positive response increased. For the study of evidence-based acupuncture, it is necessary that we conduct research on well-planned placebo acupuncture, large sample size, statistical method, method of searching and assessing literature and method of meta-analysis.

• International Journal of Fuzzy Logic and Intelligent Systems
• /
• v.7 no.4
• /
• pp.274-278
• /
• 2007

As XML becomes the standard of digital data exchange format we need to manage a lot of XML data effectively. Unlike tables in relational model XML documents are not structural. That makes it difficult to store XML documents as tables in relational model. To solve these problems there have been significant researches in relational database systems. There are two kinds of approaches: 1) One way is to decompose XML documents so that elements of XML match fields of relational tables. 2) The other one stores a whole XML document as a field of relational table. In this paper we adopted the second approach to store XML documents because sometimes it is not easy for us to decompose XML documents and in some cases their element order in documents are very meaningful. We suggest an efficient table schema to store only inverted index as tables to retrieve required data from XML data fields of relational tables and shows SQL translations that correspond to XML full-text retrievals. The functionalities of XML retrieval are based on the W3C XQuery which includes full-text retrievals. In this paper we show the superiority of our method by comparing the performances in terms of a response time and a space to store inverted index. Experiments show our approach uses less space and shows faster response times.

• The Plant Pathology Journal
• /
• v.18 no.2
• /
• pp.63-67
• /
• 2002

To understand plant-pathogen interactions, a complete set of hot pepper genes differentially expressed against pathogen attack was isolated. As an initial step, hundreds of differentially expressed cDNAS were isolated from hot pepper leaves showing non-host resistance against bacterial plant pathogens (Xanthomonas campestris pv. glycines and Pseudomonas syringae pv. syringae) using differential display reverse transcription polymerase chain reaction (DDDRT-PCR) technique. Reverse Northern and Northern blot analyses revealed that 50% of those genes were differentially expressed in pepper loaves during non-host resistance response. Among them, independent genes without redundancy were micro-arrayed for further analysis. Random EST sequence database were also generated from various CDNA libraries including pepper tissue specific libraries and leaves showing non-host hypersensitive response against X. campestris pv. glycines. As a primary stage, thousands of cDNA clones were sequenced and EST data were analyzed. These clones are being spotted on glass slide to study the expression profiling. Results of this study may further broaden knowledge on plant-pathogen interactions.