• Journal of Microbiology and Biotechnology
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• 제26권5호
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• pp.891-899
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• 2016

The recently isolated aniline-degrading bacterium Pseudomonas migulae AN-1 was tagged with green fluorescent protein (GFP) to investigate its bioaugmentation potential against aniline-contaminated groundwater through microcosm experiments. The survival and cellular response of GFP-tagged AN-1 introduced in a lab-scale aquifer corresponded directly with aniline consumption. During the process, the GFP-tagged AN-1 biomass increased from 7.52 × 10⁵ cells/ml to 128 × 10⁵ cells/ml and the degradation rate of aniline was 6.04 mg/l/h. GFP-tagged AN-1 was moderately hydrophobic (41.74%-47.69%) when treated with 20-100 mg/l aniline and exhibited relatively strong hydrophobicity (55.25%-65.78%) when the concentration of aniline was ≥100 mg/l. The membrane permeability of AN-1 increased followed by a rise in aniline below 100 mg/l and was invariable with aniline above 100 mg/l. Pyrosequencing analysis showed that the relative abundance of Proteobacteria (accounted for 99.22% in the non-bioaugmentation samples) changed to 89.23% after bioaugmentation with GFP-tagged AN-1. Actinobacteria increased from 0.29% to 2.01%, whereas the abundance of Firmicutes barely changed. These combined findings demonstrate the feasibility of removing aniline in aquifers by introducing the strain AN-1 and provide valuable information on the changes in the diversity of dominant populations during bioaugmentation.

• Journal of Microbiology
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• 제45권2호
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• pp.153-157
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• 2007

5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.

• The Plant Pathology Journal
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• 제27권1호
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• pp.53-58
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• 2011

Systemic acquired resistance is a form of inducible resistance that is triggered in systemic healthy tissues of local-infected plants. Several candidate signaling molecules emerged in the past two years, including the methylated derivatives of well-known defense hormones salicylic acid (SA) and jasmonic acid (JA). In our present study, the symptom on Cucumber mosaic virus (CMV) infected Arabidopsis leaves in 0.1 mM SA or 0.06 mM JA pre-treated plants was lighter (less reactive oxygen species accumulation and less oxidative damages) than that of the control group. JA followed by SA (JA${\rightarrow}$SA) had the highest inhibitory efficiency to CMV replication, higher than JA and SA simultaneous co-pretreatment (JA+SA), and higher than a JA or a SA single pretreatment. The crosstalk between the two hormones was further investigated at the transcriptional levels of pathogenesis-related genes. The time-course measurement showed JA might play a more important role in the interaction between JA and SA.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1993-2005
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• 2016

Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. In this study, the strain's extracellular proteome was identified by LC-MS/MS-based shotgun proteomic analysis, and their related secretory pathways were characterized by BLAST searches. A total of 513 proteins, including 100 actual secreted and 413 intracellular proteins, were detected in the extracellular proteome. The functions of these secreted proteins were elucidated and four complete secretory systems (Sec, Tat, Com, and ABC transporter) were proposed for B. pumilus. These data provide B. pumilus a comprehensive extracellular proteome profile, which is a valuable theoretical and applicative basis for future genetic modifications and development of industrial enzymes.

• KIEAE Journal
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• 제4권3호
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• pp.109-112
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• 2004

• 한국환경교육학회지:환경교육
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• 제22권4호
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• pp.26-39
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• 2009

The purpose of this study was to investigate cognitions of elementary school students of the biological resources. Subjects were 252 elementary school students in Seoul and Busan. An instrument that measures cognitions consists of 3 components: kinds of biological and non-biological resources, values of biological resources and interest of using biological resources. The three components were composed of 46 items. They had less cognition of living things than non-biological resources as resources. But, most of them conceived ecological environment to be resources. Almost 80% elementary school students thought that biological resources had values contributing to the existence and health of human beings, but less economic values except ecological values. They had cognition of biological resources which we should manage and research in. It is time to need the educational approach to integrate nature, economy and environment in environmental education. As concrete cases of biological resources are proposed, it activate elementary school students to get cognition of biological resources. In conclusion, values of biological resources will be materials for environmental education.

• 한국환경교육학회지:환경교육
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• 제13권1호
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• pp.96-110
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• 2000

There are some considerations that must take into account in environmental education in the aspect of the difference of value clarification between ecological and economic viewpoint on environment. Although we have a tendency to think that the differences are unavoidable because we are on the economy-oriented life, we should realize that such emphasis to economics comes from the differences between ecological and economic view point on environment. We have lived and thought on the basis of Economic view point, especially, environmental policies are established on the basis of economic efficiency. But this tendency has become great obstacles to environmental ethics education because it dilutes the reason of natural preservation and removes the fundamental reasons why the nature should be preserved. Therefore it is very difficult to balance the value clarification between economic and ecological viewpoint in actual life. Furthermore, environmental problems can not be solved only through economic approach, because of their limits to belief solving from providing incentives. It is very important to make people have a way of thinking which economic activities and debates can be made on the ecological resources. Therefore we can compare this relation to CHAE-YONG founded on Buddism and Chinese philosophy. CHAE means essence of every reaction in the cosmos, and YONG means the reaction itself. CHAE is regarded to ecological resources, and YONG is thought to every-day economic activities. YONG is not able to existwithout CHAE. If economic activities can be done on the basic limit of ecological resources, we can build suitable environment to living condition. We call this appropriate environment as SANG. In other words, the connection of CHAE-YONG-SANG means ecological resources - economic activities - sustainable environment. It is realized that the relations between economics and ecology should be equalized for the balanced environmental ethics education. This study tries to get out of unbalanced relations between economics and ecology from the persepectives of CHAE-YONG-SANG and it was done to suggest an alternative environmental ethics education program

• 동굴
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• 제85호
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• pp.25-27
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• 2008

Ondal Cave is important and significant in terms of cultural value and tourism. In addition, there are abundant historical and cultural resources nearby. If the geographical strength is taken advantage of, the cave would evolve into a great tourist destination and natural education spot. This paper has investigated humanistic environment of Ondal Cave in considerations for the surrounding tourism resources. There are abundant tourism resources around Ondal Cave. However, they are rarely connected with each other. In fact, they are very partially interrelated around Guinsa(temple) only. Furthermore, the number of tourists has gradually decreased. Therefore, it‘s required to further develop tourism programs and surrounding tourism resources.

• 한국가정과교육학회지
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• 제21권1호
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• pp.107-121
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• 2009

본 연구는 중학생의 남 여학생을 대상으로 객관적 및 주관적 변인에 따라 "기술 가정" 교과의 "자원의 관리와 활용" 영역에 대한 학습요구도와 활용도 차이가 있는지, 그리고 학습요구도와 활용도간에는 상관관계가 있는지를 알아보고자 하였다. 이를 위해 강릉지역의 남녀중학생 415명에게 설문지 방법을 통하여 자료를 수집하였다. 수집된 자료는 SPSS를 이용하여 Cronbach의 $\alpha$계수, 빈도, 백분율, 평균, 표준편차, t-test, ANOVA, Duncan Multiple Range's Test 등을 계산하였다. 본 연구의 결과는 다음과 같다. 1) 중학생들은 "자원의 관리와 활용" 단원에 대하여 중간이상의 학습요구도 수준을 나타냈으며, "자원의 관리와 활용" 단원에 대한 활용도는 중간보다 약간 낮은 수준이었다. 2) 객관적 및 주관적 변인에 따른 "자원의 관리와 활용" 영역의 학습요구도는 전체적으로 모의 학력, 교과관심도, 교과인식도, 교과선호도에 따라 유의한 차이가 있었다. 3) 객관적 및 주관적 변인에 따른 "자원의 관리와 활용" 단원 활용도는 전체적으로 부의 연령, 교과관심도, 교과인식도, 교과선호도와 같은 변인에 따라 유의한 차이가 있었다. 4) "자원의 관리와 활용" 단원에 대한 중학생의 학습요구도와 활용도간에는 밀접한 상관관계가 있었다.

• 한국조리학회지
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• 제11권2호
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• pp.48-66
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• 2005

This study focused on the culinary curriculums intorduced at the high school, college and university levels in order to analyze the current status and problems associated with the management of culinary related courses, and an effort was made to introduce measures to facilitate the further development of culinary education programs. This study can be summarized as follows: First, the external and internal factors which have led to the changes in the educational environment of the culinary education programs were analyzed. Second, the current state of the management of culinary education was analyzed by focusing on the vocational characteristics of the human resources presently engaged in the culinary field, the state of the management of the culinary courses offered in vocational high schools, colleges, and universities, as well as the characteristics and problems associated with the culinary curriculums at each school level. Lastly, based on the above-mentioned state of the management and the problems associated with culinary education programs, the researcher introduced four measures which were designed to facilitate the education of highly-skilled human resources in the culinary field.