• Korean Journal of Ecology and Environment
• /
• v.54 no.3
• /
• pp.209-220
• /
• 2021

Pacific herring, Clupea pallasii, a keystone species with significant ecological and commercial importance, is declining globally throughout much of its range. While traditional fishing equipment methods remain limited, new sensitive and rapid detection methods should be developed to monitor fisheries resources. To monitor the presence and quantity of C. pallasii from environmental DNA (eDNA) extracted from seawater samples, a pair of primers and a TaqMan^® probe specific to this fish based on mitochondrial cytochrome b (COB) sequences were designed for the real-time PCR (qPCR) assay. The combination of our molecular markers showed high specificity in the qPCR assay, which affirmed the success of presenting a positive signal only in the C. pallasii specimens. The markers also showed a high sensitivity for detecting C. pallasii genomic DNA in the range of 1 pg~100 ng rxn^-1 and its DNA plasmid containing COB amplicon in the range of 1~100,000copies rxn^-1, which produced linear standard calibration curves (r²=0.99). We performed a qPCR assay for environmental water samples obtained from 29 sampling stations in the southeastern coastal regions of South Korea using molecular markers. The assay successfully detected the C. pallasii eDNA from 14 stations (48.2%), with the highest mean concentration in Jinhae Bay with a value of 76.09±18.39 pg L^-1 (246.20±58.58 copies L^-1). Our preliminary application of molecular monitoring of C. pallasii will provide essential information for efficient ecological control and management of this valuable fisheries resource.

• Environmental Engineering Research
• /
• v.24 no.1
• /
• pp.127-136
• /
• 2019

Land application of treated palm oil mill effluent (TPOME) could be used as an alternative tertiary wastewater treatment process. However, phenolic compounds in TPOME might be leached to the environment. This study investigated the ability of grasses on reducing phenolic compounds in the leachate after TPOME application. Several pasture grasses in soil pots were compared after irrigating with TPOME from stabilization ponds, which contained 360-630 mg/L phenolic compounds. The number of soil bacteria in planted pots increased over time with the average of $10^8CFU/g$ for mature grasses, while only $10^4-10^6CFU/g$ were found in the unplanted control pots. The leachates from TPOME irrigated grass pots contained lower amounts of phenolic compounds and had lower phytotoxicity than that of control pots. The phenol removal efficiency of grass pots was ranged 67-93% and depended on grass cultivars, initial concentration of phenolic compounds and frequency of irrigations. When compared to water irrigation, TPOME led to an increased phenolic compounds accumulation in grass tissues and decreased biomass of Brachiaria hybrid and Brachiaria humidicola but not Panicum maximum. Consequently, the application of TPOME could be conducted on grassland and the grass species should be selected based on the utilization of grass biomass afterward.

• Journal of Convergence for Information Technology
• /
• v.9 no.7
• /
• pp.67-77
• /
• 2019

Many small and medium-sized enterprises are deploying Smart Factory without due consideration of the circumstances in which the entity is in or outside of the entity, such as environmental changes and the capabilities of its members. Therefore, utilization and effectiveness are also low after deployment. This study verifies 'the establishment of a Smart Factory through the environmental factors and absorption capabilities of small businesses' through empirical research. The survey was received by people working for small and medium-sized companies that have established Smart Factory. The results of the study showed that first, environmental factors within and outside the company had a positive effect on the absorption capacity within the company. Second, the absorption capacity within a company has had a positive effect on the deployment of a Smart Factory. Based on the above proof, it has been proved to be effective if the core areas of the Smart Factory are built on the basis of the company's internal and external environmental factors and absorption capabilities when constructing Smart Factory in small businesses. In the future, we will study the achievements of smart factory construction.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.12 no.4
• /
• pp.327-335
• /
• 2019

This study develops a prediction model for toilet and laundry water end-uses based on surveyed data which measured housing and household characteristics of 140 households over 5 years in Korea. Classical regression model assuming a normal distribution was not appropriate and estimated parameters were biased, because the distribution of measured water-uses was left-skewed. As an alternative to this problem, we considered the distribution of weibull and lognormal for each water-uses, and three regression models were compared using log-likelihood and scale parameter. As a result, weibull regression were chosen to be appropriate for both water-uses and also presented the factors that affect each water-use. This results expect that an insight is provided on water resources utilization and theoretical support role for effective water resource management.

• Mycobiology
• /
• v.47 no.1
• /
• pp.87-96
• /
• 2019

Fungi produce various secondary metabolites that have beneficial and harmful effects on other organisms. Those bioactive metabolites have been explored as potential medicinal and antimicrobial resources. However, the activities of the culture filtrate (CF) and metabolites of whiterot fungus (Schizophyllum commune) have been underexplored. In this study, we assayed the antimicrobial activities of CF obtained from white-rot fungus against various plant pathogens and evaluated its efficacy for controlling anthracnose and gray mold in pepper plants. The CF inhibited the mycelial growth of various fungal plant pathogens, but not of bacterial pathogens. Diluted concentrations of CF significantly suppressed the severity of anthracnose and gray mold in pepper fruits. Furthermore, the incidence of anthracnose in field conditions was reduced by treatment with a 12.5% dilution of CF. The active compound responsible for the antifungal and disease control activity was identified and verified as schizostatin. Our results indicate that the CF of white-rot fungus can be used as an eco-friendly natural product against fungal plant pathogens. Moreover, the compound, schizostatin could be used as a biochemical resource or precursor for development as a pesticide. To the best of our knowledge, this is the first report on the control of plant diseases using CF and active compound from white-rot fungus. We discussed the controversial antagonistic activity of schizostatin and believe that the CF of white-rot fungus or its active compound, schizostatin, could be used as a biochemical pesticide against fungal diseases such as anthracnose and gray mold in many vegetables.

• Journal of Korean Society on Water Environment
• /
• v.34 no.6
• /
• pp.669-677
• /
• 2018

Torrential rain and drought are repeated due to the increase in the unpredictable fluctuating of rainfall patterns. It is time for stabilize water resource management in terms of disaster prevention. Distributed control from sources is needed to minimize damages caused by torrential rains and droughts. Rain barrel can be used to reduce the runoff as they collect and store rainwater. In response to this situation, Seoul Metropolitan Government and other local governments implemented a project to support the installation of rain barrel and provided 90% of the cost of installing it in private areas. However, with limited budget, it is difficult to distribute rainwater to the city which is mostly covered by private areas. In this study, Samho-dong, Ulsan, where pilot projects of water cycle leading city are underway, analyzed the effects of reducing the runoff with respect to the amount of rainwater that can be used, and analyzed the economics of recoverable investment cost when installed. From the analysis, it was established that it is possible to show sufficient efficiency with a small capacity without the need to install large rain barrel effectively in the private sector, and to support the installation cost of less than 70 percent of the rainwater can be recovered.

• Journal of Korean Society for Quality Management
• /
• v.46 no.4
• /
• pp.959-972
• /
• 2018

Purpose: This study developed a simulation model that incorporates the uncertainty of demand and yield to obtain optimized results for supply chain coordination within environmental constraints. The objective of this study is to examine whether yield management for perishable products can achieve the goal of supply chain coordination between a single buyer and a single supplier under a variety of environmental conditions. Methods: We investigated the efficiency of a revenue-sharing contract and a wholesale price contract by considering demand and yield uncertainty, profit maximizing ratio, and success ratio. The implications for environmental variation were derived through a comparative analysis between the wholesale price contract and the revenue-sharing contract. We performed Monte Carlo simulations to give us the results of an optimized supply chain within the environments defined by the experimental factors and parameters. Results: We found that a revised revenue-sharing contracting model was more efficient than the wholesale price contract model and allowed all members of the supply chain to achieve higher profits. First, as the demand variation (${\sigma}$) increased, the profit of the total supply chain increased. Second, as the revenue-sharing ratio (${\Phi}$) increased, the profits of the manufacturer gradually decreased, while the profits of the retailer gradually increased, and this change was linear. Third, as the quality of yield increased, the profits of suppliers appear to increased. At last, success rate was expressed as the profit increased in the revenue-sharing contract compared to the profit increase in the wholesale price contract. Conclusion: The managerial implications of the simulation findings are: (1) a strategic approach to demand and yield uncertainty helps in efficient resource utilization and improved supply chain performance, (2) a revenue-sharing contract amplifies the effect of yield uncertainty, and (3) revised revenue-sharing contracts fetch more profits for both buyers and suppliers in the supply chain.

• Resources Recycling
• /
• v.30 no.5
• /
• pp.17-24
• /
• 2021

Natural aggregate regular exploitation has led to environmental and resource depletion issues; consequently, construction waste has become an important raw material in the supply of aggregate smoothly. The recycled aggregate produced in the most of recycled aggregate processing company in Korea has a high adhesion ratio of cement paste and mortar, which affects the water absorption ratio and density. Therefore, the quality of recycled aggregate needs to be improved. In this study, we improved the quality of recycled aggregate through the use of a multistage separation ball mill. This ball mill has a sieve which protects the ball mix and improves the motion. Products produced by using multistage separation ball mill were compared with various quality standard for utilization as recycle aggregate. Finally, we confirmed that the multistage separation ball mill can efficiently separate cement paste and mortar from natural aggregate and that it is suitable for the production of recycled aggregates.

• Animal Bioscience
• /
• v.34 no.11
• /
• pp.1757-1765
• /
• 2021

Objective: The swine leukocyte antigen (SLA) gene group, which is closely linked and highly polymorphic, has important biomedical significance in the protection and utilization of germplasm resources. However, genetic polymorphism analyses of SLA microsatellite markers in Chinese miniature pigs are limited. Methods: Eighteen pairs of microsatellite primers were used to amplify the SLA regions of seven miniature pig breeds and three wild boar breeds (n = 346) from different regions of China. The indexes of genetic polymorphism, including expected heterozygosity (He), polymorphic information content (PIC), and haplotype, were analyzed. The genetic differentiation coefficient (Fst) and neighbor-joining methods were used for cluster analysis of the breeds. Results: In miniature pigs, the SLA I region had the highest numbers of polymorphisms, followed by the SLA II and SLA III regions; the region near the centromere had the lowest number of polymorphisms. Among the seven miniature pig breeds, Diannan small-ear pigs had the highest genetic diversity (PIC value = 0.6396), whereas the genetic diversity of the Hebao pig was the lowest (PIC value = 0.4330). The Fst values in the Mingguang small-ear, Diannan small-ear, and Yunnan wild boars were less than 0.05. According to phylogenetic cluster analysis, the South-China-type miniature pigs clustered into one group, among which Mingguang small-ear pigs clustered with Diannan small-ear pigs. Haplotype analysis revealed that the SLA I, II, and III regions could be constructed into 13, 7, and 11 common haplotypes, respectively. Conclusion: This study validates the high genetic diversity of the Chinese miniature pig. Mingguang small-ear pigs have close kinship with Diannan small-ear pigs, implying that they may have similar genetic backgrounds and originate from the same population. This study also provides a foundation for genetic breeding, genetic resource protection, and classification of Chinese miniature pigs.

• Land and Housing Review
• /
• v.13 no.4
• /
• pp.125-134
• /
• 2022

Reclaimed aggregates through waste asphalt are produced and utilized for waste resource utilization. This study conducts quality tests and performance evaluations for mixtures with Rejuvenator applied to reclaimed asphalt concrete. Through quality testing and performance evaluation, the study investigates whether there is any problem in using reclaimed asphalt concrete by replacing general hox mix asphalt. As a result, the values of ordinary hot mix asphalt are similar to those of reclaimed asphalt, suggesting that the substitution does not create critical engineering issues. Using reclaimed asphalt concrete has the advantages of increasing economic efficiency and utilizing waste resources.