• 제목/요약/키워드: resistant strain

검색결과 926건 처리시간 0.025초

해양 방선균 유래 항 Methicillin Resistant Staphylococcus aureus 물질의 활성 및 상승 효과 (Activity of Anti-Methicillin Resistant Staphylococcus aureus Compound Derived Marine Actinomycetes and Its Synergistic Effect)

  • 정성윤
    • 한국해양바이오학회지
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    • 제14권2호
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    • pp.143-154
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    • 2022
  • We isolated marine actinomycetes, strain D-5 which produces anti-methicillin resistant Staphylococcus aureus (anti-MRSA) compound. Streptomyces sp. D-5 relatively grew well in the 20~25℃, pH 8.0, and NaCl 3.0%. The ethyl acetate extract of D-5 culture was separated by C18 ODS open column and reverse phase HPLC to yield anti-MRSA compound. The molecular weight of this compound was determined to be 898 by a Liquid chromatograph-mass spectrometer (LC-MS). Compared with penicillin G, this compound showed significant anti-MRSA activity. It also exhibited an inhibition zone of 26 mm at a concentration of 64 ㎍/disk and an inhibition zone of 16 mm at a concentration of 16 ㎍/disk against the MRSA KCCM 40511. Furthermore, the co-treatment of HPLC peak 5 compound and vancomycin caused a more rapid decrease in MRSA cells than each compound alone. It showed 86.8% growth inhibition activity within 12 hours at a low concentration of 50 ㎍/mL during co-treatment, and 97.1% growth in-hibition activity within 48 hours against MRSA KCCM 40511. Taken together, our results suggest that Streptomyces sp. D-5 and its anti-MRSA compound could be employed as a potent agent in MRSA infection.

Establishment of a live vaccine strain against fowl typhoid and paratyphoid

  • Cho, Sun-Hee;Ahn, Young-Jin;Kim, Tae-Eun;Kim, Sun-Joong;Huh, Won;Moon, Young-Sik;Lee, Byung-Hyung;Kim, Jae-Hong;Kwon, Hyuk Joon
    • 대한수의학회지
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    • 제55권4호
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    • pp.241-246
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    • 2015
  • To develop a live vaccine strain against fowl typhoid and paratyphoid caused by Salmonella serovar Gallinarum biovar Gallinarum (Salmonella Gallinarum) and Salmonella serovar Enteritidis (Salmonella Enteritidis), respectively, several nalidixic acid resistant mutants were selected from lipopolysaccharide (LPS) rough strains of Salmonella Gallinarum that escaped from fatal infection of a LPS-binding lytic bacteriophage. A non-virulent and immunogenic vaccine strain of Salmonella Gallinarum, SR2-N6, was established through in vivo pathogenicity and protection efficacy tests. SR2-N6 was highly protective against Salmonella Gallinarum and Salmonella Enteritidis and safer than Salmonella Gallinarum vaccine strain SG 9R in the condition of protein-energy malnutrition. Thus, SR2-N6 may be a safe and efficacious vaccine strain to prevent both fowl typhoid and paratyphoid.

토마토 유전자원의 Ralstonia solanacearum에 의한 풋마름병 저항성 평가 (Resistance Evaluation of Tomato Germplasm against Bacterial Wilt by Ralstonia solanacearum)

  • 정은주;주해진;최수연;이승엽;정용훈;이명환;공현기;이선우
    • 식물병연구
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    • 제20권4호
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    • pp.253-258
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    • 2014
  • Ralstonia solanacearum에 의해 발생하는 토마토 풋마름병에 대하여 저항성 품종이나 육성계통을 선발하기 위하여 국립농업유전자원센터로부터 국내 유전자원을 분양받아 국내 병원균에 대한 저항성 여부를 평가하였다. 한국에서 분리된 R. solanacearum SL341 균주로 52개의 품종과 계통의 저항성을 Hawaii 7996 품종과 Moneymaker 품종을 각각 저항성, 감수성 대조품종으로 사용하여 저항성 반응을 평가하였다. 그 결과 32개의 품종이 감수성이며, 10개의 품종과 계통은 저항성이 고정되지 않은 품종으로 나타났다. 5개의 시판품종과 5개의 육성 계통이 저항성 반응을 보였다. 본 연구결과를 통해 5개의 육성 계통은 저항성/방어반응의 후속연구와 저항성 품종 육종에 활용할 수 있음을 제시한다.

양식넙치(Paralichthys olivaceus)의 복수증에 관한 연구 - Edwardsiella tarde의 일부 특성과 병원성 및 대책 - (Studies on the Edwardsiellosis of cultured flounder, Paralichthys oliuaceus;- Characteristics, pathgenicity and control of Edwardsiella tarda -)

  • 한재철;김영진;서형석;김영길;이근광;안병목
    • 한국동물위생학회지
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    • 제20권1호
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    • pp.11-18
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    • 1997
  • Characteristics, pathogenicity and control of the causative organisms isolated front diseased cultured flounder, Paralichthys olivaceus were studied. The causative organisms were identified as E tarda by biochemical and biophysical characteristics. Also, it strains were named as E tarda KBF-1 and E tarda KMF-1, and optimal pH of E tarda KBF-1 and E tarda KMF-1 were 8.0, and optimal concentration of NaCl. E tarda KBF-1 was 0% and E tarda KMF-1 was 1%. In the pathogenicity test, 0~10 of the flounders of artificially infected group(E tarda KBF-1) with $1.0{\times}10^7$ cfu/fish were died within 60 hrs, but 0~9 flounders infected group with 41.0{\times}10^6$ cfu/fish were died within 60 hrs. Also, 0~10 flounders infected group(E tarda KMF-1) with $1.0{\times}10^7$ cfu/fish were died within 36 hrs, while 0~7 flounders infected with $1.0{\times}10^6$ cfu/fish were died within 60 hrs. Drug sensitivity of E tarda KBF-1 strain was resistant to AM, CF and N, and intermediate to E, K and S, and sensitivity to C, G, SxT and FF. But E tarda KMF-1 strain was resistant to CF, E and V, and intermediate to AM, C, N and SxT, and sensitivity to GM and FF.

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Prevalence and Characterization of Methicillin-Resistant Staphylococcus aureus in Raw Meat in Korea

  • Lim, Suk-Kyung;Nam, Hyang-Mi;Park, Hyun-Jung;Lee, Hee-Soo;Choi, Min-Jung;Jung, Suk-Chan;Lee, Ji-Yeon;Kim, Young-Cho;Song, Si-Wook;Wee, Sung-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제20권4호
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    • pp.775-778
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    • 2010
  • A total of 2,858 meat samples collected during 2003-2008 in Korea were investigated, and methicillin-resistant Staphylococcus aureus (MRSA) isolates were isolated from 1.0% (9/890) of beef, 0.3% (4/1,055) of pork, and 0.3% (3/913) of chicken meat samples, respectively. MRSA isolates showed the two sequence types (STs), ST72 from beef and pork and ST692 from chicken meat. MRSA isolates from beef and pork were Panton-Valentine leukocidin-negative, staphylococcal cassette chromosome mec type IVa strain with ST72, which is the most prevalent type of communityacquired MRSA in Korea. An identical pulse-field gel electrophoresis pattern was detected among 10 of 16 MRSA isolates: 9 strains from beef (n=5) and pork (n=4) in 2008, and one strain from beef in 2005.

ISOLATION OF Campylobacter jejuni AND C. coli FROM DOMESTIC AND EXPERIMENTAL ANIMALS AND THEIR DRUG SUSCEPTIBILITY

  • Nakai, Y.;Kimura, K.;Sato, M.;Inamoto, T.;Ogimoto, K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제7권4호
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    • pp.505-507
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    • 1994
  • A total of 526 domestic and experimental animals in Miyagi prefecture, Japan were investigated for fecal carriage of Campylobacter jejuni and Campylobacter coli. C. jejuni was detected in chickens (8.2%), dogs (6.3%), pigs (4.3%), cattle (1.8%) and hamsters (1.4%). C. coli was only detected from pigs (20.7%). Drug susceptibility test was performed on 5 strains of C. jejuni isolated from chickens and 13 strains of C. coli isolated from pigs to tylosin (TS), thianphenicol (TP), carbadox (CDX), chroltetracyclin (CTC), vancomycin (VCM), cefoperazone (CPZ), latamoxef (LMOX), GM were highly effective and CTC, CP and PL were moderately effective against both C. jejuni and C. coli. TS and TPH were moderately effective against C. jejuni; however, they were less effective to C. coli. One strain of C. jejuni against CTC considered to be drug resistant. The results suggest that C. jejuni and C. coli can be controlled by several drugs effectively, although a drug resistant strain exists.

Screening and Characterization of Pro biotic Lactic Acid Bacteria Isolated from Korean Fermented Foods

  • Lim, Sung-Mee;Im, Dong-Soon
    • Journal of Microbiology and Biotechnology
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    • 제19권2호
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    • pp.178-186
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    • 2009
  • To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

Resistance Mechanism of Acinetobacter spp. Strains Resistant to DW-116, a New Quinolone

  • Choi, Keum-Hwa;Baek, Moon-Chang;Kim, Byong-Kak;Choi, Eung-Chil
    • Archives of Pharmacal Research
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    • 제21권3호
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    • pp.310-314
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    • 1998
  • DW-116 is a new fluoroquinolone antimicrobial agent with a broad spectrum. In order to elucidate the resistance mechanism to DW-116 in Acinetobacter spp. bacteria, total chromosomal DNA was isolated from 10 strains of Acinetobacter spp. resistant to DW-116. Quinolone resistance determinant region (QRDR) of DNA gyrase gene was amplified by PCR. The 345 bp nucleotide fragment yielded was inserted into pKF 3 which was used as the vector. Comparisons of the DNA sequences of 8 strains with that of the wild type strain revealed a Ser-83 to Leu mutation in mutants and all ten strains contained one silent mutation$(T{\rightarrow}G)$in QRDR. From Acinetobacter MB4-8 strain, DNA gyrase was isolated and purified, through novobiocin-sepharose, heparin-sepharose affinity column chromatography. The enzyme was composed of two subunits and the molecular mass of subunits A and B were 75.6 and 51.9 kDa, respectively. The supercoiling activity of the reconstituted DNA gyrase composed of subunit A from Acinetobacter MB4-8 and subunit B from E. coli was not inhibited by $128{\mu}\textrm{g}$ml of ciprofloxacin. It might be said that one of the resistance mechanisms to DW-116 in Acinetohacter MB4-8 was subunit A alteration of DNA gyrase.

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산란계 맹장 유산균의 특성에 관한 연구 (Study on Characteristics of Lactobacillus Isolated from Hen′s Cecum)

  • 김상호;박수영;유동조;이상진;나재천;최철환;이상진;류경선
    • 한국가금학회지
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    • 제27권3호
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    • pp.227-233
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    • 2000
  • Preset study was carried out to evaluate characteristics of lactic acid producing bacteria(LAB) in hen's cecum as probiotics value. Distribution of LAB in intestinal tracts was investigated using 5∼25 weeks - old hens. So, 12 strains to LAB with different morphology were isolated purely. Acid tolerance of LAB tested at pH 1, 2, 3, and 4, and bile resistant also tested at 0, 0.3% and 0.5% bile salt concentration. Growth pattern of LAB observed to 60h. All strains of cecal LAB couldn't survive at pH 1, and decreased linearly survival colony after incubation at pH 2 although some strains could survive for 2h. Most of LAB maintained constant number at pH 3 and 4. The bacterial action could increase linearly at 0% bile salt concentration in all of tested strains. However, only one strain could multiply at 0.3% bile salt, others were influenced by bile salt. That tendency was similar at 0.5% bile salt. Growth was peaked at 12 to 18 h after innoculation. After peak, the decreasing pattern of colony was different to strains which some strains decreased rapidly or maintained for long time. The LAB of hen's cecum was similar to intolerance acidity, but different to resistant to bile salt and growth pattern by strain. So, we choose three strains which have probiocs value, and identified as Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9 and Lactobacillus vaginalis LLA11.

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A COMPARISON OF THE V4 STRAIN WITH THE CONVENTIONAL F1 AND M STRAIN OF NEWCASTLE DISEASE VACCINE IN RURAL BANGLADESH

  • Biswas, H.R.;Hoque, M.M.;Chowdhury, S.M.Z.H.;Oxley, M.E.;Rahman, M.M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제9권2호
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    • pp.231-235
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    • 1996
  • Bangladeshi indigenous chickens of mixed ages vaccinated twice at a three week interval with either conventional vaccines-$F_1$ (ocular) and M (mukteswar, Intramuscular), or heat resistant $V_4$ vaccine administered by either the ocular or oral routes, all showed satisfactory hemagglutination inhibition antibody (HI) responses and protection against Newcastle Disease (NCD) challenge persisting for four months. The antibody response to $F_1$ and M was higher than for $V_4$, which was similar whether administered by the ocular or oral routes. All vaccinated treatments have a significant level of protection compare to the control group (p<0.01). No significant difference (p>0.05) in the protection against controlled challenge with virulent NCD virus was found between vaccinated groups.