• 한국식품위생안전성학회지
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• 제9권1호
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• pp.1-13
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• 1994

A Simultaneous determination method was improved for the determination and confirmation of zeranol, zearalenone, as well as their isomers and metabolites, in beef. The analytes were extracted from tissue by CH3CN, hydrolyzed enzymatically(for glucuronide conjugates), cleaned up by a strong basic anion exchange resin combined with a liquid/liquid partitioning, derivatized using MSTFA and confirmed, quantified by GC/MS/SIM with a internal standard, zearalane. The results were as follows : (1) all the estrogens were separated on the GC/MS chromatogram under the extraction method and the chromatographic conditions improved, the retention times of zearalane-TMS2, zearalanone-TMS2, zearalenone-TMS2, zeranol-TMS3, taleranol-TMS3, and $\alpha$-zearalenol-TMS3, $\beta$-zearalenol-TMS3, were 18.49, 19.44, 19.63, 19.71, 19.79 and 19.99, 20.08 minutes, respectively. (2) The calibration curves of residual zeranol, zearalenone and their metabolites showed constantly linear(r=0.99) in the range of 5~20 ng. The minimum detection concentration of residual zeranol, zearalenone and their metabolites was 1 ppb. (3) The total average recovery of residual zeranol, zearalenone and their metabolites from spiked beef was 60.2%(CV=29.7%) at the 1 ppb and 63.5%(CV=26.5) at the 2 ppb, 72.9%(CV=18.2%) at the 4 ppb. (4) The preservation method for 6 estrogens was improved for the fast running time(21 min) and MSTFA was utilized for derivatizing 6 estrogens for improvement of recovery, for good resolution, for characteristic mass spectra unlike Jose's method and Tina's method. The utilization of zearalane as internal standard showed good quantification result for zeranol, zearalenone, as well as their isomers and metabolites, in beef.

• 분석과학
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• 제21권6호
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• pp.526-534
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• 2008

가축의 생산성 향상을 위해 투여될 수 있는 천연호르몬과 합성 호르몬제 등의 내분비계장애물질에 대하여 기존의 분석 방법을 개선 및 확립하고, 이를 토대로 국내에 유통 중인 육류 및 내장부위 중소간에 대해 호르몬 성분의 분포정도를 확인하였다. Estrogens ($17{\beta}$-estradiol, $17{\alpha}$-estradiol, estrone), androgens ($17{\beta}$-testosterone, $17{\alpha}$-testosterone), gestagens (progesterone)의 천연호르몬 6종과 합성호르몬 3종 DES, RALs (zeranol, taleranol)을 모니터링 대상 호르몬으로 GC-MS로 분석하였으며, 이로부터 호르몬의 background level을 측정하고, 상대적으로 호르몬제 등이 잔류될 수 있는 간 등의 장기에 대한 분포조사를 실시하였다. 전체 150건의 시료 중 합성 호르몬제는 검출되지 않았으며, 천연호르몬제는 내인성으로 존재하는 progesterone이 일반적인 농도 범위를 보였다.