• Restorative Dentistry and Endodontics
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• 제23권2호
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• pp.656-669
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• 1998

Hydrogen peroxide at high concentration during walking bleaching may cause damage to the tooth structure and to the surrounding periodontal tissues and may develop external root resorption. Clinically, It is so important to find a method of prevention or minimization of these complications. The efficacy of various chamber-irrigating agents to eliminate residual hydrogen peroxide after walking bleaching was examined and compared with water rinse in this study. Extracted human 46 premolars without any cementoenamel junction defects were treated endodontically and based with IRM to 1 mm below CEJ and totally bleached 3 times for each tooth with 30% hydrogen peroxide and sodium perborate. Upon completion of the 3rd walking bleaching procedure, the cervical portion and pulp chamber of each group of teeth were irrigated with catalase, 70% ethylalcohol, acetone, and distilled water. And then, a radicular hydrogen peroxide penetration was measured with spectrophotometer immediately after each bleaching and following treatment with each chamber-irrigating agents, and the significance of their eliminating efficacy of residual hydrogen peroxide was analyzed by Kruskal-Wallis test. The results were obtained as follows. 1. Cervical root penetration of hydrogen peroxide was increased as the bleaching procedure was repeated(P<.01). 2. The most effective irrigant that removed residual hydrogen peroxide was the catalase, and the least effective one was water rinsing (P<.01).; there was no significant difference between the acetone and ethanol group. 3. The Irrigation with antioxidant enzyme or water-displacement solutions can eliminate residual oxygen radicals from the pulp chamber effectively after walking bleaching. So, these agents can reduce adverse effects such as cervical external resorption and periapical inflammation and prevent residual $O_2$ from impeding composite resin polymerization, thus increase the bonding strength of composite resin. This, in turn reduces microleakage and discoloration of the esthetic restoration, extending its service-life.

• Toxicological Research
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• 제25권1호
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• pp.41-45
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• 2009

This study was conducted to monitor the level of bifenthrin residues in pear sprayed with 2% bifenthrin wettable powder (WP) at the recommended rate at four different schedules prior to harvest. The target analyte was extracted with acetone, partitioned into dichloromethane, and then purified by florisil chromatographic column. The residue determination was performed on a DB-5 capillary column using GC with electron capture detector (ECD). Linearity of this method was quite good ($r^2$ = 0.9951) in the concentration ranged from 0.2 mg/kg to 10 mg/kg. Recovery test was carried out at two concentration levels, 0.2 mg/kg and 1.0 mg/kg, in three replicates, and their rates were from 82.9% to 107.2%. No quantitative bifenthrin was detected in pear of all kinds of treatments including the treatment sprayed 4 times until 7 days before harvest. This sensitive and selective method can be used to monitor the trace residual amounts of bifenthrin in pear in a quite low concentration level.

• BMB Reports
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• 제32권3호
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• pp.254-258
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• 1999

The NADPH-dependent succinic semialdehyde reductase is one of the key enzymes in the brain GABA shunt, and it catalyzes the formation of the neuromodulator $\gamma$-hydroxybutyrate from succinic semi aldehyde. This enzyme was inactivated by diethylpyrocarbonate (DEP) with the second-order rate constant of $1.1{\times}10^3\;M^{-1}min^{-1}$ at pH 7.0, $25^{\circ}C$, showing a concomitant increase in absorbance at 242 nm due to the formation of N-carbethoxyhistidyl derivatives. Complete inactivation of succinic semialdehyde reductase required the modification of five histidyl residues per molecule of enzyme. However, only one residue was calculated to be essential for enzyme activity by a statistical analysis of the residual enzyme activity. The inactivation of the enzyme by DEP was prevented by preincubation of the enzyme with the coenzyme NADPH but not with the substrate succinic semialdehyde. These results suggest that an essential histidyl residue involved in the catalytic activity is located at or near the coenzyme binding site of the brain succinic semialdehyde reductase.

• 콘크리트학회논문집
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• 제23권4호
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• pp.431-440
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• 2011

콘크리트 구조물은 화재에 노출시 고온에 의해 내부 구조가 변화하며 보유하고 있는 강도와 변형 성능이 저하되어 최종적으로 수명이 단축하게 된다. 그 성능 저하 수준은 도달된 온도, 고온에 노출된 시간, 콘크리트의 배합, 골재의 특성 및 콘크리트 자체의 특성 등에 의해 결정된다. 이 연구는 물시멘트비, 잔골재율 및 굵은 골재의 최대 크기등의 변수에 대한 초고강도 콘크리트의 열적 거동을 평가하기 위해 실시되었다. 상온 및 $500^{\circ}C$의 온도에 대하여 초음파 속도, 동탄성 계수, 정탄성 계수 및 압축강도 시험은 ${\varnothing}100{\times}200\;mm$ 원주형 콘크리트 시험체를 사용하여 실시되었다. 결과로서 $500^{\circ}C$의 온도에서 가열된 초고강도 콘크리트의 잔존 역학적 특성은 물결합재비, 잔골재율 및 굵은 골재 최대 치수의 변화에 영향을 받는 것으로 나타났다.

• 농약과학회지
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• 제19권1호
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• pp.14-21
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• 2015

인삼의 재배환경에 따른 잔류특성을 조사하고 살포 농약의 행적을 알아보기 위하여 5년근 인삼포에 덮개 유무와 인삼 줄기의 움직임으로 생긴 구멍을 흙으로 메우는 방법 여부의 서로 다른 조건에서 azoxystrobin (20% SC)을 안전사용기준의 기준량과 배량농도로 수확 7일전까지 10일 간격으로 4회 살포하였으며, 행적 구명 시험을 위해서는 기준량을 1회 살포한 후 분석시료를 채취하였다. 재배기간 중 덮개를 설치하고 구멍을 막은 경우가 잔류량이 낮아 덮개 설치와 구멍 메우기가 잔류량 감소에 기여하는 것으로 판단되었다. 인삼에 살포한 azoxystrobin은 비표면적이 큰 잎에 가장 많이 분포하였으며, 살포시기가 인삼의 수확기에 근접할수록 잎 중 잔류량은 감소하였으나 토양표면의 검출량은 증가하였다. 뇌두를 포함한 인삼 뿌리 중 시험농약의 분포율은 약 0.1%이었으며, 인삼 섭취 시 뇌두를 제거할 경우 12.7-20.4%(평균 16.6%)의 잔류농약 섭취를 감소시키는 효과가 있었다.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.483-488
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• 2014

In order to improve the expression of heat-resistant xylanase XYNB from Aspergillus niger SCTCC 400264, XynB has been cloned into Pichia pastoris secretary vector pPIC9K. The XynB production of recombinant P. pastoris was four times that of E. coli, and the $V_{max}$ and specific activity of XynB reached $2,547.7{\mu}mol/mg$ and 4,757 U/mg, respectively. XynB still had 74% residual enzyme activity after 30 min of heat treatment at $80^{\circ}C$. From the van der Waals force analysis of XYNB (ACN89393 and AAS67299), there is one more oxygen radical in AAS67299 in their catalytic site, indicating that the local cavity is much more free, and it is more optimal for substrate binding, affinity reaction, and proton transfer, etc, and eventually increasing enzyme activity. The H-bonds analysis of XYNB indicated that there are two more H-bonds in the 33rd Ser of XYNB (AAS67299) than in the 33rd Ala(ACN89393 ), and two H-bonds between Ser70 and Asp67.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1856-1862
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• 2015

In order to improve the stability of endoglucanase under thermal and acidic conditions, the endoglucanase gene was fused to the N-terminus of the Saccharomyces cerevisiae pir gene, encoding the cell wall protein PIR. The fusion gene was transformed into Pichia pastoris GS115 for expression. A resulting strain with high expression and high activity was identified by examining resistance to Geneticin 418, Congo red staining, and quantitative analysis of enzyme activity. SDS-PAGE analysis revealed that the endoglucanase was successfully displayed on the yeast cell surface. The displayed endoglucanase (DEG) showed maximum activity towards sodium carboxyl methyl cellulose at approximately 275 IU/g cell dry weight. DEG exhibited greater than 60% residual activity in the pH range 2.5-8.5, higher than free endoglucanase (FEG), which had 40% residual activity at the same pH range. The highest tolerated temperature for DEG was 70℃, much higher than that of FEG, which was approximately 50℃. Moreover, DEG showed 91.1% activity at 65℃ for 120 min, while FEG only kept 77.8% residual activity over the same period. The half-life of DEG was 270 min at 65℃, compared with only 150 min for FEG. DEG could be used repeatedly at least three times. These results suggest that the DEG has broad applications as a yeast whole-cell biocatalyst, due to its novel properties of high catalytic efficiency, acid-thermal stabilities, and reusability.

• 정보과학회 논문지
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• 제42권12호
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• pp.1495-1502
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• 2015

센서 네트워크에서 압축은 종단 간 지연시간과 에너지 사용량 사이의 이율배반적인 관계가 있다. 데이터의 크기를 줄이기 위해 압축을 하면, 추가적인 지연시간과 에너지 소비가 발생하지만, 전송으로 인한 에너지 소모는 감소하게 된다. 일반적으로, 배터리기반 센서 네트워크에서는 지연시간을 손해 보더라도 네트워크의 생존시간을 최대화하기위해 압축을 널리 사용하고 있다. 한편 태양 에너지 기반 센서 네트워크에서는 주기적으로 에너지 재생산이 이루어짐에 따라, 동작하는데 충분한 에너지양 이상의 에너지가 존재할 가능성이 있다. 본 논문에서는 여분의 에너지를 사용해 종단 간 지연시간을 감소시키는 에너지 적응형 선택적 압축 기법을 제안한다. 제안하는 기법은 노드의 에너지가 충분하지 않을 때, 에너지 소비를 줄이기 위해 압축을 사용하고, 에너지가 충분한 경우에는 종단 간 지연시간 감소를 위해 압축을 사용하지 않는다. 시뮬레이션을 통한 에너지와 지연시간의 성능평가를 통하여 제안하는 기법의 우수성을 증명하였다.

• Corrosion Science and Technology
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• 제7권4호
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• pp.208-211
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• 2008

Safety operation of main pipelines is primarily provided by anticorrosive monitoring. Anticorrosive monitoring of oil pipeline transportation objects is based on results of complex corrosion inspections, analysis of basic data including design data, definition of a corrosion residual rate and diagnostic of general equipment's technical condition. All the abovementioned arrangements are regulated by normative documents. For diagnostics of corrosion-technical condition of oil pipeline transportation objects one presently uses different methods such as in-line inspection using devices with ultrasonic, magnetic or another detector, acoustic-emission diagnostics, electrometric survey, general external corrosion diagnostics and cameral processing of obtained data. Results of a complex of diagnostics give a possibility: $\cdot$ to arrange a pipeline's sectors according to a degree of corrosion danger; $\cdot$ to check up true condition of pipeline's metal; $\cdot$ to estimate technical condition and working ability of a system of anticorrosive protection. However such a control of corrosion technical condition of a main pipeline creates the appearance of estimation of a true degree of protection of an object if values of protective potential with resistive component are taken into consideration only. So in addition to corrosive technical diagnostics one must define a true residual corrosion rate taking into account protective action of electrochemical protection and true protection of a pipeline one must at times. Realized anticorrosive monitoring enables to take a reasonable decision about further operation of objects according to objects' residual life, variation of operation parameters, repair and dismantlement of objects.

• 농약과학회지
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• 제17권4호
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• pp.244-248
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• 2013

홍고추와 고춧가루 중 친환경 유기농자재 matrine의 잔류특성을 구명하고 가공계수를 산출하기 위하여 시험포장에 matrine의 시판제품(유효성분 2%)을 1,000배 희석하여 조제한 살포액을 7일 간격으로 2회 살포 한 후 최종 약제 살포 당일부터 7일차까지 경시적으로 홍고추를 채취하였다. 채취한 홍고추는 $60^{\circ}C$의 열풍건조기를 이용하여 수분함량이 14% 이하가 되도록 건조한 후 마쇄하여 고춧가루를 조제하였다. 시험작물 중 matrine의 회수율은 106.6-119.1%이었으며, 저장 안정성 시험의 회수율은 106.6-113.1%이었다. 1회 처리구 홍고추와 고춧가루 중 matrine의 잔류량은 각각 < 0.01-0.11과 0.03-0.25 mg/kg이었으며, 2회 처리구의 경우 0.02-0.12와 0.04-0.40 mg/kg이었다. 또한 고춧가루 중 matrine의 가공계수는 1.50-3.33으로 건조 후 잔류량은 증가하였다.