• Genomics & Informatics
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• v.21 no.3
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• pp.39.1-39.15
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• 2023

DNA barcoding without assessing reliability and validity causes taxonomic errors of species identification, which is responsible for disruptions of their conservation and aquaculture industry. Although DNA barcoding facilitates molecular identification and phylogenetic analysis of species, its availability in clariid catfish lineage remains uncertain. In this study, DNA barcoding was developed and validated for clariid catfish. 2,970 barcode sequences from mitochondrial cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes and D-loop sequences were analyzed for 37 clariid catfish species. The highest intraspecific nearest neighbor distances were 85.47%, 98.03%, and 89.10% for COI, Cytb, and D-loop sequences, respectively. This suggests that the Cytb gene is the most appropriate for identifying clariid catfish and can serve as a standard region for DNA barcoding. A positive barcoding gap between interspecific and intraspecific sequence divergence was observed in the Cytb dataset but not in the COI and D-loop datasets. Intraspecific variation was typically less than 4.4%, whereas interspecific variation was generally more than 66.9%. However, a species complex was detected in walking catfish and significant intraspecific sequence divergence was observed in North African catfish. These findings suggest the need to focus on developing a DNA barcoding system for classifying clariid catfish properly and to validate its efficacy for a wider range of clariid catfish. With an enriched database of multiple sequences from a target species and its genus, species identification can be more accurate and biodiversity assessment of the species can be facilitated.

• Genomics & Informatics
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• v.21 no.4
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• pp.47.1-47.12
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• 2023

Silver barb (Barbonymus gonionotus) is among the most economically important freshwater fish species in Thailand. It ranks fourth in economic value and third in production weight for fisheries and culture in Thailand. An XX/XY sex-determination system based on gynogenesis was previously reported for this fish. In this study, the molecular basis underlying the sex-determination system was further investigated. Genome-wide single-nucleotide polymorphism data were generated for 32 captive-bred silver barb individuals, previously scored by phenotypic sex, to identify sex-linked regions associated with sex determination. Sixty-three male-linked loci, indicating putative XY chromosomes, were identified. Male-specific loci were not observed, which indicates that the putative Y chromosome is young and the sex determination region is cryptic. A homology search revealed that most male-linked loci were homologous to the Mariner/Tc1 and Gypsy transposable elements and are probably the remnants of an initial accumulation of repeats on the Y chromosome from the early stages of sex chromosome differentiation. This research provides convincing insights into the mechanism of sex determination and reveals the potential sex determination regions in silver barb. The study provides the basic data necessary for increasing the commercial value of silver barbs through genetic improvements.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.04a
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• pp.2-3
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• 1999

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2001.12a
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• pp.123-123
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• 2001

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.288.1-288
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• 1998

No Abstract, See Full Text

• Proceedings of the Botanical Society of Korea Conference
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• 1999.04a
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• pp.13-13
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• 1999

• Nuclear Engineering and Technology
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• v.52 no.5
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• pp.975-983
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• 2020

Since the Fukushima Daiichi accident in 2011, concerns for the safety of multi-unit Nuclear Power Plant (NPP) sites have risen. This is because more than 70% of NPP sites are multi-unit sites that have two or more NPP units and a multi-unit accident occurred for the first time. After this accident, Probability Safety Assessment (PSA) has been considered in many countries as one of the tools to quantitatively assess the safety for multi-unit NPP sites. One of the biggest concerns for a multi-unit accident such as Fukushima is that the consequences (health and economic) will be significantly higher than in the case of a single-unit accident. However, many studies on multi-unit PSA have focused on Level 1 & 2 PSA, and there are many challenges in terms of public acceptance due to various speculations without an engineering background. In this study, two kinds of multi-unit Level 3 PSA for multi-unit site have been carried out. The first case was the estimation of multi-unit risk with conservative assumptions to investigate the margin between multi-unit risk and QHO, and the other was to identify the effect of time delays in releases between NPP units on the same site. Through these two kinds of assessments, we aimed at investigating the level of multi-unit risk and understanding the characteristics of risk in a multiunit context.

• Nuclear Engineering and Technology
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• v.50 no.8
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• pp.1255-1265
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• 2018

The need for a PSA (Probabilistic Safety Assessment) for a multi-unit at a site is growing after the Fukushima accident. Many countries have been studying issues regarding a multi-unit PSA. One of these issues is the problem of many combinations of accident sequences in a multi-unit PSA. This paper deals with the methodology and software to quantify a PSA scenarios for a multi-unit site. Two approaches are developed to quantify a multi-unit PSA. One is to use a minimal cut set approach, and the other is to use a Monte Carlo approach.

• Nuclear Engineering and Technology
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• v.50 no.8
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• pp.1217-1233
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• 2018

Following a surge of interest in multi-unit risk in the last few years, many recent studies have suggested methods for multi-unit probabilistic safety assessment (MUPSA) and addressed several related aspects. Most of the existing studies though focused on two-unit nuclear power plant (NPP) sites or used rather simplified probabilistic safety assessment (PSA) models to demonstrate the proposed approaches. When considering an NPP site with three or more units, some approaches are inapplicable or yield very conservative results. Since the number of such sites is increasing, there is a strong need to develop and validate practical approaches to the related MUPSA. This article provides several detailed approaches that are applicable to multi-unit Level 1 PSA for sites with up to six or more reactor units. To validate the approaches, a multi-unit Level 1 PSA model is developed and the site core damage frequency is estimated for each of four representative multi-unit initiators, as well as for the case of a simultaneous occurrence of independent single-unit initiators in multiple units. For this purpose, an NPP site with six identical OPR-1000 units is considered, with full-scale Level 1 PSA models for a specific OPR-1000 plant used as the base single-unit models.

• Nuclear Engineering and Technology
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• v.50 no.8
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• pp.1246-1254
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• 2018

The importance of performing Level 3 probabilistic safety assessments (PSA) along with a general interest in assessing multi-unit risk has been sharply increasing after the Fukushima Daiichi nuclear power plant (NPP) accident. However, relatively few studies on multi-unit Level 3 PSA have been performed to date, reflecting limited scenarios of multi-unit accidents with higher priority. The major difficulty to carry out a multi-unit Level 3 PSA lies in the exponentially increasing number of multi-unit accident combinations, as different source terms can be released from each NPP unit; indeed, building consequence models for the astronomical number of accident scenarios is simply impractical. In this study, a new approach has been developed that employs the look-up table method to cover every multi-unit accident scenario. Consequence results for each scenario can be found on the table, established with a practical amount of effort, and can be matched to the frequency of the scenario. Preliminary application to a six-unit NPP site was carried out, where it was found that the difference between full-coverage and cut-off cases could be considerably high and therefore influence the total risk. Additional studies should be performed to fine tune the details and overcome the limitations of the approach.