• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.2
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• pp.205-215
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• 2008

The purpose of this study is to evaluate at which stage of tooth germ would develop into normal calcification and hence to increase the success rate of transplantation. Therefore, tooth germs on the 15th, 17th embryonic day and the 3rd day of birth were separated for allotransplantation into maxilla of adult rat of 11 weeks. Calcification processes were analyzed radiographically and histopathologically at 4 weeks and 8 weeks after allotransplantation. The results are as follows: 1. Allotransplanted tooth germ at 4 weeks and 8 weeks showed delayed calcification compared to that of normal odontogenesis. 2. At 4 weeks, abnormal calcified tissue, such as odontoma and ankylosis of osteodentin with surrounding alveolar bone were observed. 3. At 8 weeks, allotransplanted tooth germs of the 15th and 17th embryonic day showed calcification and osteodentin surrounded by periodontal ligament. 4. At 8 weeks, allotransplanted tooth germs of the 3rd day of birth showed calcification composed of cementum and osteodentin. In this study, we observed small sized and amorphous calcified tissue from allotropic allotransplantation of tooth germs. Since these calcified tissue were underdeveloped and shaped irregularly, for calcification into normal tooth form, further study needs consideration about the reduction of surgical trauma, developmental stage of transplanted tooth germ, blood supply from recipient site, fixation method in transplanted site and period of transplantation.

• Journal of Bio-Environment Control
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• v.26 no.2
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• pp.100-107
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• 2017

This experiment was carried out to analyze the effects of substrate reuse on the growth and yield of summer paprika in cyclic hydroponics. The test group was divided into a new coco slab, one year reused coco slab two year reused coco slab based on 30% nutrient solution reuse, and was performed from April 18 to November 31, 2016 for 30 weeks. As a result, plant height of early growth was that the 2 year reused slab was longer than the new slab but the final growth period was 56.58 cm shorter. First group flower position was that reused slab was shorter by 2.92 cm than the new slab and the second group flower position was 0.31 cm long. The relative internode length of early growth, when the reused slab was used, the imbalance in the late growth stage was increased compared with the use of the new slab. The number of growth nodes in the 1 and 2 year reused slab was the smallest with 27.4 nodes. However, the number of harvested nodes did not show the difference in the test group, and the ratio of harvested that the 2 year reused slab was the highest at 26.8%. The ratio of unmarketable fruit tended to increase as the growth progressed. Fresh weight was 227.7g for new slab, 219.2g for 2 year reused slab and 21.2g for 1 year reused slab. The dry weight of the new slab increased with the reuse of the slab. It was 17.13g for new slab, 18.26g for 1 year reused, and 19.28g for 2 year reused. The average water content of the entire growth period was smaller as the slab was reused, and the 1 year reused slab was about 20g less than the 2 year reused slab. This trend was steadily occurring throughout the entire growing season. Especially, the reused slab for 1 year was less than 60g after 3 groups compared to other test groups. In conclusion, If will control seriously occurrence of unmarketable fruits by weakening after medium growth in summer-cultivated paprika in EC-based recycling hydroponic cultivation with reused cocopeat substrate, It is not what I have to worry that decrease of the yield and deterioration of the quality due to the change of physical and chemical properties of the slab and the pathogenic bacteria infection.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.3
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• pp.33-46
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• 2020

This study analyzes the entrepreneurship education phenomena for agri-food entrepreneurs whose main business is the production of agricultural products and the sale of processed products, using the qualitative study Strauss & Corbin(1998)'s evidence theory approach. From the entrepreneur's point of view, I would like to summarize the phenomena that appear in education, and to prepare a theoretical basis for explaining the phenomena. The importance of entrepreneurship education is emphasized to cultivate the ability to develop and provide products tailored to customers. The necessity of education leads to an increase in demand according to the situational awareness of the founders, and the quantitative increase in entrepreneurship education in the agri-food sector is a clear trend. Inevitably, the need for various discussions on systematic and effective entrepreneurship education is raised. For the study, an interview was conducted with preliminary or entrepreneur who have experienced entrepreneurship education in the agri-food sector. As a research method, I use Strauss & Corbin(1998)'s approach and analyze qualitative data using QSR's NVIVO 12 program. Through this study, it was found that contextual and systematic entrepreneurship education in the agri-food sector has the effect of strengthening competitiveness and strengthening sales. There is a need for follow-up management of trainees. Strengthening the competitiveness of start-ups is based on training professional manpower through education and linking regions with cities. Strengthening sales is based on product planning and market development. This study explores entrepreneurship education in the agri-food sector, which has not been actively conducted in the past. Exploratory analysis on the experiences of the founders of agri-food sector as education demanders has an important meaning for understanding the phenomenon of start-up education.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.3
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• pp.121-132
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• 2020

University Students go through the development process of expertise in a single field based on theoretical knowledge they have learned in the university. Their plans to develop their careers could lead to a shift in business as they choose to find jobs and start businesses throughout their lives. The attitude of career decision making process has been changed from learning in the form of receiving and memorizing existing knowledge to learning in a more proactive and autonomous manner, and there is a growing need for informal learning. In this study, the informal learning is investigated to clarify the impact on the employment competency and entrepreneurial intention. New implications are deduced by the introduction of start-up business to the traditional approach based on the employment ability. In addition, the importance of team-work has been increased in the field of eduction and business. The impact on the employment competency and entrepreneurial intention is studied based on the satisfaction level of team-based learning. Survey was conducted on 163 university students in North Chungcheong Province from March to June 2019 and the results of the study were summarized as follows. First, it has been confirmed that the informal learning of university students has a significant impact on their employment competency, but not on their entrepreneurial intention. Second, the employment competencies have perfect mediating effect on the informal learning and the entrepreneurial intention. Third, the satisfaction on the team-based learning did not have a significant impact on the employment competency of university students by informal learning, but it had a positive effect on the dependency between employment competency and entrepreneurial intention. In these days, start-up education at universities has been strengthened. Throughout this research, the need for informal learning is confirmed to enhance the employment competency and entrepreneurial intention of the university education program. In addition, team-based learning is introduced actively to utilize a variety of information from the university students.

• Journal of Wetlands Research
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• v.19 no.2
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• pp.230-239
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• 2017

These days, the demand of farm tours is on the rise in Korea. However, most of them are related to farming experience. To overcome the situation, ecological experience is introduced. In this sense, it is necessary to obtain the contents and educational materials of ponds, the space of farming and ecological experience. Therefore, this study analyzed the use patterns of oriental medicine of pond wetland plants. Oriental medicine is used for old and traditional knowledge, and is still applied to various fields, such as medical science, pharmacy, and science. The analyzed result of this study will be helpful for the educational materials of ecological experience. This study surveyed plants of 40 pond wetlands in rural areas, and looked into the use patterns of traditional medicine by using the contents of Korea Traditional Knowledge Portal being operated by Korean Intellectual Property Office. According to the vegetation survey of the 40 pond wetlands, there were a total of 457 taxa in 108 families, 309 genera, 392 species, 59 varieties, 5 forma, and 1 subspecies. Among them, 314 taxa in 53 families, 136 genera, 265 species, 45 varieties, 3 forma, and 1 subspecies had the information on the use patterns of traditional medicine. It means that 68.8% of the plants around pond wetlands contain traditional medicine. Given other application patterns, such as food, handcraft and traditional farming, more than 70~80% are expected to include traditional knowledge. 314 taxa used for traditional medicine had 570 kinds of efficacy and 325 kinds of disease treatment. It means that one taxon has 4.0 (0~20) kinds of efficacy on average and 6.6(0~20) kinds of disease treatment. It is expected that this study result will be applied to various fields, including experience, education, and medical science. And it is considered that valuable pond wetlands are worthwhile to be conserved constantly.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.889-894
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• 1998

This study was designed as a part of efforts to investigate the biochemical pollutant markers for diagnosis of marine pollutions by changes in oxygen radicals and their scavenger enzymes of the flounder (Paralichthys olivaceus) in the South Sea of Korea. Protein contents in brain and muscle of cultured flounder in the South Sea were remarkably lower ($19\~42\%$ and $25\~41\%$, respectively) than those of wild flounder in Pohang of the East Sea as a control group. Lipid Peroxide (LPO) levels in serum of cultured in the South Sea were considerably higher ($5\~33\%$) Elan those of wild flounder in Pohang. Hydroxyl radical formations in serum of cultured flounders in the South Sea were lower ($4\~25\%$) than those of wild flounders in Pohang. Superoxide dismutase (SOD) activities in serum of cultured flounders in South Sea were also significantly lower ($7\~31\%$) than those of wild flounders in Pohang. It is believed that significant decrease of protein contents in brain and muscle, and remarkable increases of LPO in serum of cultured flounders may be used as a biochemical pollutant markers for diagnosis of marine pollutions. These results suggest that near-coastal water as well as neritic water of the South Sea might be affected by pollutant input as well as the Yellow Sea of Korea.

• Journal of Animal Science and Technology
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• v.51 no.5
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• pp.353-360
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• 2009

When 14 microsatellite (MS) markers were applied in the identifying test for 480 Hanwoo, the discriminating power was estimated as $3.43{\times}10^{-27}$ based on the assumption of a random mating group (PI). This rate is 1,000 times higher than that of 60 single nucleotide polymorphism (SNP) markers. On the other hand, the power of the 60 SNP markers was estimated as $4.69{\times}10^{-20}$ and $8.02{\times}10^{-12}$ on the assumption of a half-sib mating group ($PI_{half-sibs}$) and a full-sib mating group ($PI_{sibs}$), respectively. These powers were 10 times and 10,000 times higher than those of the 14 MS markers. The results indicated that the total number of alleles (MS vs SNP = 146 vs 120) acted as a key factor for the discriminating power in a random mating population, and the total number of markers (MS vs SNP = 14 vs 60) was a dominant influence on the power in half-sib and full-sib populations. In the Hanwoo population, in which it was assumed that the entire population is the enormous half-sib group formed by the absolute genetic contribution of a few nuclear bulls, there will be only a 10 times difference in the discriminating power between the 14 MS markers and the 60 SNP makers. However, the probability of not excluding a candidate parent pair from the parentage of an arbitrary offspring, given that only the genotype of the offspring ($PNE_{pp}$) was 1,000 times higher as shown by the 14 MS markers than that by the 60 SNP markers. The strong points of SNP makers are the stability of the variation (low mutation rate) and automation of high-throughput genotyping. In order to apply these merits for the practical and constant Hanwoo identity test, research and development are required to set a cost-effective platform and produce a homemade apparatus for SNP genotyping.

• The Korean Journal of Pesticide Science
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• v.10 no.3
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• pp.172-182
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• 2006

This study was designed to assess molinate fate in the lysimeter by measuring the total radioactivity in the leachate, evolved $^{14}CO_2$, and $^{14}C$-residues in soil and rice plant. The amounts of applied $^{14}C$ in the leachate from the lysimeter for 20 weeks were 1.05% in 2.31 pore volume (217,465 mL) at the first and 0.34% in the second year, respectively. The amount of $^{14}CO_2$ evolved from the lysimeter accounted for 6.47% and 0.03% of applied $^{14}C$ in the first and second year. The $^{14}C$-activities in the soil layer of the lysimeter were distributed 18.0% (1st) and 13.3%(2nd) in the depth of 0 to 10 cm, 4.3 (1st) and 1.1% (2nd) in the depth of 10 to 20 cm. Most of the applied $^{14}C$ was detected in the top 20 cm soil layer. Total $^{14}C$ in rice plants grown at lysimeter were detected 11.46% of applied $^{14}C$. 11.11% in straw, 0.24% in brown rice grain, 0.08% in chaff and 0.03% in ears were distributed in the first year. Consequently, environmental fate of molinate using lysimeter simulating a paddy rice field were investigated 25.24% in soil, 11.64% in rice plant, 1.05% in leachate, 6.74% in evolved $^{14}CO_2$ and 0.02% in volatilized organic chemicals in the first year.

• Korean Journal of Food Science and Technology
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• v.17 no.6
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• pp.495-499
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• 1985

To develope a process by which liquid foods can be stored in the liquid state at the frozen storage temperature, suitable cryoprotectants were selected. Orange juice, chosen as an example of liquid foods, was stored with combined cryoprotectants at $-15^{\circ}C$, and quality changes of orange juice during storage were evaluated. Among 7 cryoprotectants tested, NaCl solution had lower initial freezing point than others, and initial freezing points of glucose, fructose, glycerol, propylene glycol and citric acid were close to each other. Considering flavor quality of orange juice, cryoprotectants suitable for reducing freezing point of orange juice were glucose, fructose, glycerol, and citric acid. Combined cryoprotectants for reducing freezing point of 3 and 4 folds concentrated orange juice to $-15^{\circ}C$ consisted of 10% glucose, 8% frutose, 4.6% glycerol and 3% citric acid, and 5.5% glucose, 4.5% fructose, 4.6% glycerol, and 3% cirtric acid, respectively. When destruction of ascorbic acid, sedimentation volume and sensory flavor score of orange juice stored with combined cryoprotectants at $-15^{\circ}C$ and the control stored at $-18^{\circ}C$ were compared, there were no significant differences. These results indicated that liquid foods with suitable combined cryoprotectants could be stored at $-15^{\circ}C$ or below in the liquid state without adverse effect on quality of the stored products.

• Journal of Life Science
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• v.26 no.1
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• pp.34-41
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• 2016

Black chokeberries (scientific name Aronia melanocarpa) have been reported to have major effects due to anti-oxidant, anti-inflammatory, and anti-cancer capabilities. In this study, we investigated the anti- wrinkle effects of A. melanocarpa, including collagenase inhibition effects and their molecular biological mechanisms, such as oxidative stress-induced matrix metalloproteinase (MMP), mitogen-activated protein (MAP) kinase, and activator protein (AP)-1 expression and/or phosphorylation. In collagenase inhibition activity, the ethyl acetate fraction of black chokeberry (AE) was 77.2% at a concentration of 500 μg/ml, which was a significant result compared to that of Epigallocatechin gallate (positive control, 83.9% in 500 μg/ml). In the reactive oxygen species (ROS) assay, the AE produced 78% of ROS in 10 μg/ml and 70% of ROS in 75 μg/ml, which was a much lower percentage than the ROS production of H₂O₂-induced CCRF S-180II cells. In the MTT assay, cell viability was increased dose-dependently with AE in H₂O₂-induced cells. In protein expression by western blot assay, the AE suppressed the expression and phosphorylation of MMPs (MMP-1, -3, -9), MAPK (ERK, JNK, and p38), and AP-1 (c-Fos and c-Jun), and expressed the pro-collagen type I in H₂O₂-induced cells. These results suggest that black chokeberries have anti-wrinkle and collagen-production effects, and they may be used in applications for material development in the functional food and cosmetic industries.