• 제목/요약/키워드: reproductive potential

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오카다익산 군 독소: 독성, 분석법 및 관리 동향 (Okadaic Acid Group Toxins: Toxicity, Exposure Routes, and Global Safety Management)

  • 이경아;김남현;김장균;김연정;이정석;한영석
    • 한국식품위생안전성학회지
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    • 제38권6호
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    • pp.409-419
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    • 2023
  • Okadaic acid (OA) 군 독소는 설사성 패류중독(diarrhetic shellfish poison, DSP)을 유발하는 해양생물독소이다. 설사성패독은 오카다익산(OA)과 그 유사체인 디노피시스톡신(DTX)으로 구성되어 있으며, 주로 와편모조류에서 생성되어 이매패류의 체내에 축적되어 독화된다. 이에 EFSA, WHO에서는 안전관리를 위하여 수산물 내 OA군 독소 함량 기준을 설정하였다. 최근 우리나라 연안에서도 원인조류인 Dinophysis sp.의 출현이 보고된 바 있으며, 국내 수산물 생산과 소비에도 영향을 미치고 있다. 또한 국제적인 움직임에 발맞추어 국내에서도 2022년 설사성 패독 기준 관리 대상 물질에 DTX2를 추가하여 관리하고 있다. 본 연구는 OA군 독소의 이화학적 특성, 분석법, 인체 노출 사례와 국내외 관리 현황 등의 자료를 검토하여 OA군 독소의 체계적인 모니터링과 안전관리의 기반을 마련하고자 수행되었다.

Hsp90 Inhibitor Induces Cell Cycle Arrest and Apoptosis of Early Embryos and Primary Cells in Pigs

  • Son, Myeong-Ju;Park, Jin-Mo;Min, Sung-Hun;Hong, Joo-Hee;Park, Hum-Dai;Koo, Deog-Bon
    • Reproductive and Developmental Biology
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    • 제35권1호
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    • pp.33-45
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    • 2011
  • Heat shock protein 90 (Hsp90) is ATPase-directed molecular chaperon and affects survival of cancer cell. Inhibitory effect of Hsp90 by inducing cell cycle arrest and apoptosis in the cancer cell was reported. However, its role during oocyte maturation and early embryo development is very insufficient. In this study, we traced the effects of Hsp90 inhibitor, 17-allylamino-17-demethoxygeldanamycin (17-AAG), on meiotic maturation and early embryonic development in pigs. We also investigated several indicators of developmental potential, including structural integrity, gene expression (Hsp90-, cell cycle-, and apoptosis-related genes), and apoptosis, which are affected by 17-AAG. Then, we examined the roles of Hsp90 inhibitor on viability of primary cells in pigs. Porcine oocytes were cultured in the NCSU-23 medium with or without 17-AAG for 44 h. The proportion of GV arrested oocytes was significantly different between the 17-AAG treated and untreated group (78.2 vs 34.8%, p<0.05). After completion of meiotic maturation, the proportion of MII oocytes was lower in the 17-AAG treated group than in the control group (27.9 vs 71.0%, p<0.05). After IVF, the percentage of penetrated oocytes was significantly lower in the 17-AAG treated group (25.2%), resulting in lower normal pronucleus formation (2PN of 14.6%). Therefore, the inhibition of meiotic progression by Hsp90 inhibitor played a critical role in fertilization status. Porcine embryo were cultured in the PZM-3 medium with or without 17-AAG for 6 days. In result, significant differences in developmental potential were detected between the embryos that were cultured with or without 17-AAG. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) showed that the number of containing fragmented DNA at the blastocyst stage increased in the 17-AAG treated group compared with control (7.5 vs 4.4, respectively). Blastocysts that developed in the 17-AAG treated group had low structural integrity and high apoptotic nuclei than those of the untreated control, resulting in decrease the embryonic qualities of preimplantation porcine blastocysts. The mRNA expressions of cell cycle-related genes were down-regulated in the 17-AAG treated group compared with control. Also, the expression of the pro-apoptotic gene Bax increased in 17-AAG treated group, whereas expression of the anti-apoptotic gene Bel-XL decreased. However, the expression of ER stress-related genes did not changed by 17-AAG. Cultured pESF cells were treated with or without 17-AAG and used for MIT assay. The results showed that viability of pESF cells were decreased by treatment of 17-AAG ($2{\mu}M$) for 24 hr. These results indicated that 17-AAG decreased cell proliferation and increased cell death. Expression patterns Hsp90 complex genes (Hsp70 and p23), cell cycle-related genes (cdc2 and cdc25c) and apoptosis-related genes (Bax and Bcl-XL) were significantly changed by using RT-PCR analysis. The spliced form of pXbp-1 product (pXbp-1s) was detected in the tunicamycin (TM) treated cells, but it is not detected in 17-AAG treated cells. In conclusion, Hsp90 appears to play a direct role in porcine early embryo developmental competence including structural integrity of blastocysts. Also, these results indicate that Hsp90 is closely associated with cell cycle- and apoptosis-related genes expression in developing porcine embryos.

안면도 소나무 채종원 교배양식 추정모수의 연간비교 (Two-Year Estimates of Mating System in Seed Orchard of Pinus densiflora Revealed by cpSSR and nSSR Markers)

  • 김영미;홍용표;박재인
    • 한국산림과학회지
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    • 제104권4호
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    • pp.578-587
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    • 2015
  • 교배양식 유전모수를 확인하기 위하여 nuclear SSR (nSSR) 표지와 chloroplast SSR (cpSSR) 표지를 이용하여 2006년과 2007년에 안면도 소나무 채종원(77년 조성)에서 생산된 종자를 대상으로 타가교배율과 화분오염율, 근친교배율을 확인하였다. cpSSR 유전형에 근거한 타가교배율은 2006년에 94.9~100%(평균 98.9%)이며, 2007년에는 91.2~100%(평균 97.7%)이다. nSSR 유전자형에 근거한 타가교배율은 2006년에 90.3~100%(평균 95.9%), 2007년에 81.6~100%(평균 95.3%)의 타가교배율이 산출되었다. 두 표지를 동시에 비교하여 확인한 결과 2006년 생산종자의 평균 누적 타가교배율 100%, 2007년 생산종자의 평균 누적 타가교배율은 98.9%로 추정되었다. 근친교배율($t_m-t_s$: biparental inbreeding)은 2006년에 -0.006과 2007년에 0.007으로 추정되었다. 평균 화분오염율은 2006년에 평균 48.9%, 2007년에 평균 42.4%이며, 종자의 cpSSR 유전형을 근거로 확인한 화분친 기여율(기여화분친 수)은 2006년에 0.458(평균 16.2개), 2007년에 0.512(평균 14.8개)로 확인되었다. 결론적으로, 2006년, 2007년 안면도 소나무 채종원(77년 조성) 내 클론간 높은 타가교배율이 확인됨으로써 채종원산 종자의 유전적 품질은 자가교배로 인한 근교약세가 원인이 되는 불량형질이 발생할 가능성이 낮을 것으로 기대된다. 안면도 소나무 채종원(77년 조성) 내 교배양식 연간 분석을 통해서 확인된 결과가 향후 진전세대 채종원 조성 및 관리에 유용한 정보를 제공할 것으로 기대된다.

포유동물의 생식과 페로몬 (Mammalian Reproduction and Pheromones)

  • 이성호
    • 한국발생생물학회지:발생과생식
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    • 제10권3호
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    • pp.159-168
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    • 2006
  • 설치류를 포함한 대부분의 포유동물은 페로몬 반응을 중개하는 두 개의 화학감각 시스템(chemosensory system)을 갖고 있는데, 각각 주후각시스템(main olfactory system, MOS)과 부후각시스템(accesory olfactory system, AOS)이다. MOS에 속하는 화학감각뉴런들은 주후각 상피 내에 위치하며, AOS에 속하는 화학감각뉴런들은 비강 윗부분의 서골비기관(vomeronasal organ, VNO)에 위치한다. 공기 중의 비휘발성 페로몬 성분들은 구개 위쪽으로 열린 관을 통해 VNO의 내강으로 이동한다. 페로몬 수용체 단백질들은 크게 두 개의 슈퍼패밀리 V1R과 V2R로 나뉘는데, 이들은 구조적으로 큰 차이가 있으며 MOS에서 발현되는 후각 수용체들과는 무관하다. 이들은 7개의 막관통 도메인을 갖는 G-단백질 결부 단백질(seven transmembrane domain G-protein coupled proteins, V1R은 $G_{{\alpha}i2}$와, 그리고 V2R은 $G_{0\;{\alpha}}$와 연관)이다. V2R은 비고전적 MHC Ib 유전자 산물인 M10과 기타 8개의 M1 패밀리 단백질들과 함께 작용한다. 그 외 VNO 뉴런의 중요한 구성 분자는 TrpC2로, 이는 transient receptor potential(TRP)의 양이온 채널 단백질이며 세포내 신호전달과정에서 중요한 역할을 할 것으로 추정된다. 포유동물의 화학적 의사소통과정에서 페로몬은 작용 모드 또는 효과에 따라 4종류로 분류할 수 있는데, 프라이머(primer), 신호자(signaler), 조정자(modulator) 그리고 방출자(releaser)이다. 근본적으로 이들 화학신호에 대한 반응들은 개체 간, 심지어는 한 개체 내에서도 다양할 수 있다. 이러한 다양성은 페로몬이 스테로이드 호르몬들과 함께 또는 단독으로, 신경전달물질들과 같은 비스테로이드 요인들의 후각정보 처리 과정에 미치는 각종 조절의 차이에 의해 나타날 수 있다. 이러한 조절은 유리한 사회적, 환경적인 조건들을 갖도록 수용자의 생식 축에 미치는 영향을 증강 또는 촉진한다. 가장 좋은 예는 수컷 생쥐의 소변 중의 테스토스테론 의존적인 주요 요단백질(major urinary proteins, MUPs)에 의한 임신방지효과(Bruce 효과)이다. 흥미롭게도 생쥐 GnRH 뉴런은 냄새와 페로몬 양자 모두로부터 페로몬 신호를 수용하는 것 같다. 비록 상당한 논란의 소지는 있지만, 그간의 연구들은 생식과 기타 여러 기능들 사이에 복잡한 상호교차 관계가 있음을 시사한다. 여기서 GnRH 뉴런은 다양한 원천으로부터의 정보를 통합하고, 다시 다양한 뇌기능을 조절하는 것으로 보인다.

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한국가족계획사업(韓國家族計劃事業)의 문제점(問題點) (Problems in the Korean National Family Planning Program)

  • 홍종관
    • Clinical and Experimental Reproductive Medicine
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    • 제2권2호
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    • pp.27-36
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    • 1975
  • The success of the family planning program in Korea is reflected in the decrease in the growth rate from 3.0% in 1962 to 2.0% in 1971, and in the decrease in the fertility rate from 43/1,000 in 1960 to 29/1,000 in 1970. However, it would be erroneous to attribute these reductions entirely to the family planning program. Other socio-economic factors, such as the increasing age at marriage and the increasing use of induced abortions, definitely had an impact on the lowered growth and fertility rate. Despite the relative success of the program to data in meeting its goals, there is no room for complacency. Meeting the goal of a further reduction in the population growth rate to 1.3% by 1981 is a much more difficult task than any one faced in the past. Not only must fertility be lowered further, but the size of the target population itself will expand tremendously in the late seventies; due to the post-war baby boom of the 1950's reaching reproductive ages. Furthermore, it is doubtful that the age at marriage will continue to rise as in the past or that the incidence of induced abortion will continue to increase. Consequently, future reductions in fertility will be more dependent on the performance of the national family planning program, with less assistance from these non-program factors. This paper will describe various approaches to help to the solution of these current problems. 1. PRACTICE RATE IN FAMILY PLANNING In 1973, the attitude (approval) and knowledge rates were quite high; 94% and 98% respectively. But a large gap exists between that and the actual practice rate, which is only 3695. Two factors must be considered in attempting to close the KAP-gap. The first is to change social norms, which still favor a larger family, increasing the practice rate cannot be done very quickly. The second point to consider is that the family planning program has not yet reached all the eligible women. A 1973 study determineded that a large portion, 3096 in fact, of all eligible women do not want more children, but are not practicing family planning. Thus, future efforts to help close the KAP-gap must focus attention and services on this important large group of potential acceptors. 2. CONTINUATION RATES Dissatisfaction with the loop and pill has resulted in high discontinuation rates. For example, a 1973 survey revealed that within the first six months initial loop acceptance. nearly 50% were dropouts, and that within the first four months of inital pill acceptance. nearly 50% were dropouts. These discontinuation rates have risen over the past few years. The high rate of discontinuance obviously decreases the contraceptive effectiveness. and has resulted in many unwanted births which is directly related to the increase of induced abortions. In the future, the family planning program must emphasize the improved quality of initial and follow-up services. rather than more quantity, in order to insure higher continuation rates and thus more effective contraceptive protection. 3. INDUCED ABORTION As noted earlier. the use of induced abortions has been increase yearly. For example, in 1960, the average number of abortions was 0.6 abortions per women in the 15-44 age range. By 1970. that had increased to 2 abortions per women. In 1966. 13% of all women between 15-44 had experienced at least one abortion. By 1971, that figure jumped to 28%. In 1973 alone, the total number of abortions was 400,000. Besides the ever incre.sing number of induced abortions, another change has that those who use abortions have shifted since 1965 to include- not. only the middle class, but also rural and low-income women. In the future. in response to the demand for abortion services among rural and low-income w~men, the government must provide and support abortion services for these women as a part of the national family planning program. 4. TARGET SYSTIi:M Since 1962, the nationwide target system has been used to set a target for each method, and the target number of acceptors is then apportioned out to various sub-areas according to the number of eligible couples in each area. Because these targets are set without consideration for demographic factors, particular tastes, prejudices, and previous patterns of acceptance in the area, a high discontinuation rate for all methods and a high wastage rate for the oral pill and condom results. In the future. to alleviate these problems of the methodbased target system. an alternative. such as the weighted-credit system, should be adopted on a nation wide basis. In this system. each contraceptive method is. assigned a specific number of points based upon the couple-years of protection (CYP) provided by the method. and no specific targets for each method are given. 5. INCREASE OF STERILIZA.TION TARGET Two special projects. the hospital-based family planning program and the armed forces program, has greatly contributed to the increasing acceptance in female and male sterilization respectively. From January-September 1974, 28,773 sterilizations were performed. During the same time in 1975, 46,894 were performed; a 63% increase. If this trend continues, by the end of 1975. approximately 70,000 sterilizations will have been performed. Sterilization is a much better method than both the loop and pill, in terms of more effective contraceptive protection and the almost zero dropout rate. In the future, the. family planning program should continue to stress the special programs which make more sterilizations possible. In particular, it should seek to add the laparoscope techniques to facilitate female sterilization acceptance rates. 6. INCREASE NUMBER OF PRIVATE ACCEPTORS Among the current family planning users, approximately 1/3 are in the private sector and thus do not- require government subsidy. The number of private acceptors increases with increasing urbanization and economic growth. To speed this process, the government initiated the special hospital based family planning program which is utilized mostly by the private sector. However, in the future, to further hasten the increase of private acceptors, the government should encourage doctors in private practice to provide family planning services, and provide the contraceptive supplies. This way, those do utilize the private medical system will also be able to receive family planning services and pay for it. Another means of increasing the number of private acceptors, IS to greatly expand the commercial outlets for pills and condoms beyond the existing service points of drugstores, hospitals, and health centers. 7. IE&C PROGRAM The current preferred family size is nearly twice as high as needed to achieve a stable poplation. Also, a strong boy preference hinders a small family size as nearly all couples fuel they must have at least one or more sons. The IE&C program must, in the future, strive to emphasize the values of the small family and equality of the sexes. A second problem for the IE&C program to work. with in the: future is the large group of people who approves family planning, want no more children, but do not practice. The IE&C program must work to motivate these people to accept family planning And finally, for those who already practice, an IE&C program in the future must stress continuation of use. The IE&C campaign, to insure highest effectiveness, should be based on a detailed factor analysis of contraceptive discontinuance. In conclusion, Korea faces a serious unfavorable sociodemographic situation- in the future unless the population growth rate can be curtailed. And in the future, the decrease in fertility will depend solely on the family planning program, as the effect of other socio-economic factors has already been maximumally felt. A second serious factor to consider is the increasing number of eligible women due to the 1950's baby boom. Thus, to meet these challenges, the program target must be increased and the program must improve the effectiveness of its current activities and develop new programs.

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가축의 개량 및 번식효율 증진에 관한 연구 I. 토끼에 있어서 체외수정능획득 정자에 의한 체외수정 및 수정란 번식에 관한 연구 (Studies on the Improvement of Performance and Reproductive Efficiency in Farm Animals I. In vitro fertilization by in vitro capacitated sperm and transfer of in vitro fertilized embryos in rabbits)

  • 정영채;김창근;주일영;정길생;이규승
    • 한국가축번식학회지
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    • 제10권2호
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    • pp.192-203
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    • 1986
  • Two experiments in this study were designed to compare the potential for in vitro capacitation and in vitro fertilization of ejaculated sperm among individual rabbit bucks. In experiment 1, for in vitro capacitation, the ejaculated sperm were preincubated in DM for 12 hr or 18 hr after HIS treatment, then 12 hr -or 18 hr- preincubated sperm were incubated with superovulated rabbit ova in a 5% CO2 incubator for 36 hr at 38$^{\circ}C$, and a part of cleaved ova was transferred to the recipient does for implantation of embryo. In experiment 2, effect of lysolecithin addition to preincubation medium on induction of accelerated in vitro capacitation and in vitro fertilization of individual rabbit sperm was studied. Experiment 1; 1. Percent acrosome reaction of sperm, noted after staining, after 12 hr or 18 hr preincubation ranged from 52.5 to 76.0% and from 67.5 to 90.0%, respectively and sperm motility index of these sperm ranged from 20.0 to 47.5 for 12 hr-preincubated sperm and from 15.0 to 37.5 for 18 hr- preincubated sperm. There was no a certain relation between percent acrosome reaction and sperm motility index. 2. In vitro fertilization rate (cleavage rate) of in vitro capacitated sperm varied widely among individual bucks, ranging from 0 to 47.8% for 12 hr - preincubated sperm and from 0 to 60.9% for 18 hr -prein- cubated sperm. Cleavage rate of 18 hr - preincubated sperm was higher and faster than that of 12 hr - preincubated sperm. 3. Eight of 44 in vitro fertilized embryos transferred into 6 recipients were implanted in 4 recipients (66.7%) up to day 15 and implnatation rate was 18.2%. Experiment 2; 1. The percent acrosome reaction of sperm before and after 4 hr preincubation in DM without lysolecithin varied significantly among individual bucks, ranging from 0.4 to 18.4% and from 1.7 to 37.4%, respectively and percent acrosome reaction of sperm at 30 min after addition of 60${\mu}$g/ml lysolecithin also was significantly different among bucks, ranging from 19.2 to 67.1%. 2. Effect of accelerated acrosome reaction following lysolecithin addition was more considerable in the individuals showed less percent acrosome reaction before and after 4 hr preincubation. Percentage of motile sperm and motility score showed a trendency towards a decrease with increase of preincubation time and time after lysolecithin addition. 3. In vitro fertilization rate (cleavage rate) at 24 hr postinesmination with pooled sperm were treated to 60 $\mu\textrm{g}$/ml lysolecithin for 30 min after 4 hr preincubation was 24.6%, a higher rate than 13.2% for control. While 80 $\mu\textrm{g}$/ml lysolecithin-added sperm showed a lower cleavage than control and 60$\mu\textrm{g}$/ml-added sperm at both 24 hr and 48 hr postinsemination. These results from 2 experiments suggest that more useful preincubation time for the in vitro capacitation of ejaculated rabbit sperm is 18 hr in DM after HIS treatment, although there is wide variation in vitro capacitation and in vitro fertilization rate among individual bucks, and lysolecithin addition to at least 4 hr - preincubated sperm in DM can result in almost same in vitro fertilization rate as that of 18 hr - preincubated sperm in the experiment 1.

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파월국군장병의 고엽제 위해에 관한 예비적 역학조사 (A Preliminary Epidemiologic Study on Korean Veterans Exposed to Herbicides in Vietnam War)

  • 김정순;임현술;이홍복;이원영;박영주;김성수
    • Journal of Preventive Medicine and Public Health
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    • 제27권4호
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    • pp.711-734
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    • 1994
  • Among chemical agents in herbicides, dioxin (2, 3, 7, 8-tetrachloro dibenzo-$\rho$-dioxin : TCDD), a chemical contaminant in herbicides sprayed during the Vietnam War has been known to be the major agent causing toxic effects. Approximately 320,000 korean soldiers participated the Vietnam War from 1964 to 1974. Although the potential hazards of the herbicides among Korean veterans exposed were implicated, the problem had not been a public issue until 1991 when Korean veterans were informed U.S. companies, the herbicides manufacturer payed fund, from which a trust fund for New Zealand and Australian Class members were established in 1985. After a series of appeals and demonstration by the Korean Veterans demanding medical care and compensation for their serious health damages, a bill of medical care and compensation for herbicides victims was promulgated in March 1993 and become effective from May 1993, This study was carried out with two major objectives : the first to understand the health problems caused from the herbicides by reviewing literatures published, and the second to examine the nature and extent of health impacts among Korean veterans exposed and to develop valid study methods for the major study by interviewing and reviewing records on a part of veterans (638 persons) registered and completed medical examination in Seoul Veterans Administration Hospital from June to October 1993. The results obtained are as followings: 1. The literature review of 107 papers revealed that 1) Dioxin is teratogenic, carcinogenic and affects almost all organs including nervous, endocrine, and reproductive systems in animal experiments. 2) The diseases showing evidence of causal association were Hodgkin's disease, non-Hodgkin's disease, lung cancer, lymphoma, soft tissue sarcoma, chloroacne and polyneuropathy when judged on the basis of consistency in study results and biological plausibility. 2. Interview and medical record review study on 638 veterans, though limited validity owing to lack of control group, crude estimates of dioxin exposure levels (no biomarkers measurable), and uncertainty of diagnosis, showed that: 1) Most of the study subject's were in their 40's of age and had been dispatched to Vietnam during the period from $1965{\sim}1970$ around one year. 2) Most frequently complained symptoms in medical examination were motor weakness (32%), sensory abnormalities in extremities (23%), skin diseases (22%), and pain in extremities (20%) whereas in Interview they were more frequent in order of skin problem (44%), motor weakness (38%), sensory abnormalities and pain in extremities(17% and 19% each). Kappa indices on the same category of complaints between two sources of information were variable and relatively low. 3) On medical examination, only a part of the 638 subjects had initial impression (442 pts) and final diagnosis (218 pts) suggesting decision making on diagnosis appeared to be difficult even with all available modern medical technologies: in initial impression disorders from peripheral and central neuropathy were predominant whereas in final diagnosis various types of skin disorder were most frequent 4) When dose-response relationship between several conditions (from questionnaire) and arbitrary exposure scores were examined by CMH linear trend test, spontaneous abortion, sexual problems and health problem of offsprings showed statistically significant linear trends. However, pregnancy, accident and suicidal attempts did not show any relationship in this study capacity. 5) Among complaints, psychosis and neurosis (anxiety, phobia) in interview study, and memory disorder and psychosis in medical record study revealed linear trend. 6) Skin disorder was the only condition showing linear trend in initial impression and none in final diagnosis on medical examination. Even though objective to select out dioxin-related disease or group of diseases from this study was not achieved the research experiences provided firm basis for developing various methodological approaches. 3. From this preliminary study we concluded that a larger scale major epidemiologic study on health impacts of herbicides among Korean veterans exposed is not only Indispensible but also well designed study with more valid exposure information and diagnosis may be able to establish causal relationship between certain groups of diseases and exposure to the herbicides among Korean veterans.

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Neospora caninum에 자연 감염된 임신우에서 IFN${\gamma}$, 항체 및 호르몬 수준의 상관관계 (Relationship Between IFN${\gamma}$ Production, Antibody and Hormone Levels in Naturally Neospora caninum-infected Pregnant Dairy Cows)

  • 손정훈;박배근;손화영;정주영;박상준;김태환;조성환;류시윤
    • 한국임상수의학회지
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    • 제27권6호
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    • pp.640-646
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    • 2010
  • 네오스포라 감염증은 세포내성 원충성 기생충인 Neospora caninum에 의해 발생하는 질병으로 전세계적으로 발생하고 있으며, 소에서는 번식장해로 인한 막대한 경제적 손실을 유발한다. 본 연구는 4 곳의 각기 다른 목장으로부터 N. caninum에 자연 감염된 양성우와 음성우를 포함한 26두의 임신우를 선정하여 2주 내지 4주 간격으로 혈액을 채취하여 호르몬, 항체가, IFN${\gamma}$ 수준과 유산 혹은 분만과의 상관관계를 조사하였다. 유산하지 않은 Neospora 양성우14두의 S/P 평균값은 분만 전 15주에 최고치를 보였고, 이후 분만 때까지 감소하였다. 유산한 양성우 3두의 유산 시기는 각각 인공 수정 후 20주 (142일), 26주 (185일), 28주 (199일)였으며, 이들의 S/P값은 유산 전 13주부터 유산 때까지 높은 수준을 유지하였다. 유산하지 않은 양성우에서 IFN${\gamma}$ 수준은 개체에 따라 그리고 동일한 개체라도 임신기간이 경과함에 따라 다양하게 나타났으며, 유산한 양성우에서 IFN${\gamma}$ 수준은 계속 높은 상태를 유지하거나 또는 유산시점에 근접하여 급상승하였다. Neospora 음성우 9두의 IFN${\gamma}$ 수준은 양성우보다 낮았지만, 그 양상은 유산하지 않은 양성우와 유사하였다. 유산하지 않은 양성우와 음성우에서 평균적인 progesterone수준은 분만 전 7주부터 뚜렷하게 감소하였다. 유산한 양성우에서는 유산 전 5주와 7주의 평균적인 progesterone 수준은 정상 분만한 음성우나 양성우의 분만전 5주와 7주에 측정된 평균 progesterone 수준보다 낮았다. 유산한 양성우에서 17${\beta}$-estradiol의 평균 수준은 유산 시점에 근접할수록 증가하였지만, 혈청내 총양은 유산하지 않은 양성우와 음성우에서 분만시점에 근접하여 측정한 평균 17${\beta}$-estradiol 수준보다 더 낮았다. 이상의 결과로 미루어 보아 Neospora에 감염된 소에서 17${\beta}$-estradiol과 progesterone 수준의 저하는 IFN${\gamma}$ 산생을 증가시켜, 결국에는 유산을 초래할 수 있다고 생각된다.

Demecolcine 처리에 의한 탈핵과 수핵란 세포질의 세포 주기가 소 핵이식란의 발육에 미치는 영향 (Effect of Demecolcine-Assisted Enucleation and Recipient Cell Cycle Stage on the Development of Nuclear Transfer Bovine Embryos)

  • 백진주;박춘근;양부근;김정익;정희태
    • Reproductive and Developmental Biology
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    • 제29권3호
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    • pp.175-180
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    • 2005
  • 본 연구는 demecolcine 처리에 의한 탈핵과 수핵란 세포질의 세포 주기가 소 체세포 핵이식란의 발육에 미치는 영향을 검토하였다. 체외에서 $16\~20$시간 성숙배양된 난자를 극체 방출 유무 및 MI, MII기 난자로 구분하여 $0.4\;\muL/mL$ demecolcine으로 40분간 처리 후 염색체 부위가 돌출된 난자는 탈핵 후 핵이식에 공시하였다. 소의 귀 피부 세포를 탈핵란에 이식하여 전기융합과 활성화 처리(Ca-ionophore+DMAP)를 거쳐 체외 배양하였다. Demecolcine처리 후 $86.2\%$의 난자가 염색체 부위의 돌출을 보여 이 중 $98.8\%$가 탈핵에 성공하였다. Demecolcine은 핵이식란의 발육에 영향을 주지 않았다. 제1극체 방출란 유래 핵이식란의 배반포 발육율은 극체 미방출란 유래 핵이식란에 비하여 유의적으로 높았다($18.2\%\;vs.\;4.6\%\cdot$, P<0.05). 한편, MI 난자 유래 핵이식란의 분할율 및 배반포 발육율은($69.4\%$$5.9\%$) MII 난자 유래 핵이식란에 비하여 유의적으로 낮았다($96.7\%$$23.9\%$, P<0.05). 본 연구의 결과는 demecolcine 처리가 소 난자의 탈핵에 매우 효과적이며 MII기 난자가 MI기 난자에 비하여 수핵란 세포질로 더 적절하나 극체 미방출란 및 MI기 난자도 비록 제한적이기는 하지만 핵이식란의 배반포 발육을 지원할 수 있음을 보여준다.

$UPLC^{TM}$를 이용한 화장품 중 보존제 8종(파라벤 6종, 페녹시에탄올, 클로페네신)의 동시분석 (Simultaneous Determination of 8 Preservatives (6 Parabens, 2-Phenoxyethanol, and Chlorphenesin) in Cosmetics by $UPLC^{TM}$)

  • 박정은;이소미;정혜진;장이섭
    • 대한화장품학회지
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    • 제33권4호
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    • pp.263-267
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    • 2007
  • 미생물의 오염을 통해 화장품이 변질되거나 분해되는 것을 방지하여 소비자를 보호하기 위해 화장품에 보존제가 사용된다. 파라벤류는 제형화하기 쉽고, 활성 범위가 넓으며, pH에 화학적으로 안정하면서 저렴하여 거의 모든 종류의 화장품에 널리 사용된다. 페녹시에탄올과 클로페네신 역시 화장품에 일반적으로 사용되는 보존제로 보통 파라벤과 함께 사용된다. 파라벤의 독성은 일반적으로 낮지만, 손상된 피부에는 자극을 유발할 수 있으며, estrogenic 잠재성, 마취 효과 및 생식 독성의 가능성에 대한 논란이 있어 왔다. 따라서 파라벤은 배합한도 원료로 지정 관리되고 있으며, 페녹시에탄올과 클로페네신도 마찬가지로 최대 허용량이 지정되어 있다. 그러므로 제품 중 보존제의 함량을 관리하는 것은 중요하다. 그러나 일반적으로 사용되는 역상 액체크로마토그래프법으로는 이성질체를 포함한 6종의 파라벤과 페녹시에탄올 및 클로페네신을 동시에 분리 분석하기 어려웠다. 용출 시간이 길어져, 피크 모양이 나쁘고 분리능이 좋지 않아 정확한 정량이 불가능하였다. 본 연구에서는 ultra performance liquid $chromatography^{TM}\;(UPLC^{TM}$)를 이용하여 8종의 보존제를 10 min 이내에 동시분석을 시도하였다. 또한, International conference on harmonisation (ICH) 가이드라인의 밸리데이션 방법에 근거하여 본 시험법의 적합성을 검증하고, 로션, 팩트, 파운데이션 및 립글로스 등 다양한 제형에 적용이 가능함을 보였다. 본 시험법은 파라벤류를 포함한 다양한 보존제를 함유한 화장품 중 보존제의 함량을 단시간에 간편하고 정확하게 정량하는데 활용될 수 있을 것이다.360 nm)에서 3개의 피이크로 분리되었다. 분리된 3가지 성분은 luteolin, quercetin 및 kaempferol이었으며, 그들의 성분비는 각각 18.24 %, 58.79 %, 22.97 %로 quercetin의 함량이 가장 큰 것으로 나타났다. 루이보스 추출물의 ethylacetate 분획의 TLC 크로마토그램은 7개의 띠로 분리되었고, HPLE 크로마토그램은 9개의 피이크를 보여주었다. TLC와 HPLC의 띠와 피이크를 확인한 결과, HPLC의 9개의 피이크는 용리순서로 peak 1 (조성비 14.71 %)은 isoorientin, peak 2 (28.84 %)는 orientin peak 3 (5.63 %)은 vitexin, peak 4 (12.73 %)는 rutin과 isovitexin, peak 5 (9.24 %)는 hyperoside, peak 6 (5.40%)은 isoquercitrin, peak 7 (1.48 %)은 luteolin, peak 8 (17.61 %)은 quercetin 및 peak 9 (4.59 %)는 kaempferol로 확인되었다. Aglycone 분획은 elastase 저해활성($IC_{50}$)이 $9.08\;{\mu}g/mL$로 매우 큰 활성을 나타내었다. 이상의 결과들은 루이보스 추출물이 $^1O_2$ 혹은 다른 ROS를 소광시키거나 소거함으로써 그리고 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리키며, 루이보스 성분에 대한 분석과 ethylacetate 분획의 당 제거 실험 후 얻어진 aglycone 분획의 큰 elastase 저해활성으로부터