• Asian-Australasian Journal of Animal Sciences
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• 제10권6호
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• pp.609-613
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• 1997

The reproductive performance of forty two male broilers divided into three similar groups and fed on isocaloric and isonitrogneous diets containing 0, 10 or 20% water washed neem seed kernel meal (WWNSKM) was investigated from 20 to 32-wks of age. Results on semen characteristics revealed that feeding of WWNSKM led to significant (p < 0.05) reduction in semen volume, sperm concentration associated with increased incidences of morphological abnormalities in the spermatozoa when compared to that of the control birds. A drastic reduction in the fertilizing ability of spermatozoa was observed, the adverse effects being more at higher inclusion level of the cake. Hatchability of eggs also declined in the WWNSKM fed group. Histological examination of testes revealed a higher number of degenerating cells and poor spermatogenesis along with multinucleated giant cells in the seminiferous tubules of the testes of birds receiving the high dose of WWNSKM in diet. It may be concluded that the feeding of WWNSKM by incorporating in isocaloric and isonitrogneous diets to cockerels is associated with adverse effect on their fertility.

• 대한한의학회지
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• 제20권1호
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• pp.22-29
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• 1999

To evaluate the effect of Choakwiyeum water extract on the male reproductive functions, experiments were performed on male rats. The results obtained were as follows: 1. Intromission frequency was increased significantly after the administration of Choakwiyeum water extract in male rats. 2. Intromission latency was increased significantly after the administration of Choakwiyeum water extract and ketoconazole in male rats. 3. Mount frequency was increased significantly after the administration of Cboakwiyeum water extract and TCDD in male rats. 4. Serum testosterone concentration was increased significantly after the administration of Choakwiyeum water extract in male rats. 5. Serum testosterone concentration was increased significantly after the administration of Choakwiyeum water extract and ketoconazole in male rats.

• Journal of Ginseng Research
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• 제6권1호
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• pp.84-99
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• 1982

Water content and its seasonal change in reprodltctive organs were reviewed in relation to cultivation practice s. Precipitati on and humidity under shade roof were reviewed in relation to shading ,jystem and environmental factors. High water content of reproductive organs suggests vulnerability to water stress during reproductive growth stage. Watering during dehisconce treat menu seems to keep optimum temperature but cnoventional practice seems to be too often In watering. Information effe on water physiology of seeds is too rare to develop seed storing method and ctive seed use. Dehiscent mechanism was considered in terms of water absorption of embryo. Precipitation rate of conventional shade roof reaclled to 38% and at line level 50% and varied with shade patterns. Precipitation rate under shade has been investigated for itself but should be investigated in relation to light intensity and soil moisture content Relative humidity under shade depends mainly on air humidity and soil moisture, considerably on shade materials and lithe on pole height, bed width or plant density. Since relative humidity was lower in afternoon it was often less than 50% even in summer with high temperature suggesting possible disorder of phi biological function especially in photosynthesis. More information was needed on optimum humidity for productive physiological function of leaf.

• 한국발생생물학회지:발생과생식
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• 제23권4호
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• pp.355-365
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• 2019

The morphogenetically matured spermatozoa (sperm) are generated in the testes by the spermatogenesis. They travel male reproductive tract with many substances secreted from the accessory reproductive organs. One of the substances is the semenogelin (SEMG) released from the seminal vesicles that is involved in the post-testicular maturation. The expression of SEMG gene was investigated in seminal vesicle tissues of sexually matured and regressed male Syrian hamsters by reverse transcription polymerase chain reaction (RT-PCR). The SEMG gene was uniquely identified in the seminal vesicles of the matured Syrian hamsters and compared to the genes reported previously. But the expression of SEMG gene was not observed in reproductively and completely regressed testes of Syrian hamsters. These results indicate that the expressions of the SEMG gene are related to the reproductive capability in the male Syrian hamsters.

• 환경생물
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• 제22권4호
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• pp.550-558
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• 2004

The effects of postnatal exposure to octylphenol(OP) on the expressions of the steroidogenic enzymes and testosterone production were evaluated. Postnatal male mice (15-day-old) were injected with 2 or 20mg $kg^{-l}$ body weight (BW) of OP for 5 days and sacrificed on postnatal day 21. Testosterone concentration was measured by radioimmunoassay and the expressions of the testicular genes were determined by RT-PCR analyses. Significant reductions in the mean body and testis weight were observed in the OP treated animals. No marked alteration in the histological structure of the testis were observed, however, slight reduction in the seminiferous tubule diameter and the number of Leydig cells and several pyknotic cells could be identified in the 20 mg $kg^{-l}$ BW of the OP treated animals. Serum testosterone concentration was dramatically reduced and the mRNA expressions of the steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc) and $17\beta$-hydroxylase/Cl7-20 lyase $(P450_{17\alpha})$ were decreased. No significant changes of the gene expressions of the steroidogenic factor-l (SF-I) and estrogen and androgen receptor after the OP treatment showed that the decreased expressions of the steroidogenic enzymes in the present study did not correlate with these genes. Altogether, the present study demonstrates that postnatal treatment of OP inhibits steroidogenesis by decreasing the transcriptional expressions of the StAR and steroidogenic enzymes. The alteration in steroidogenesis may adversely affect the normal development of the testis and sper- matogenesis.

• 한국동물생명공학회지
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• 제34권4호
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• pp.311-317
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• 2019

Reproductive potential decreases with age. A decrease in male fertility is due to a combination of morphological and molecular alterations in the testes. Transient receptor potential vanilloid receptor-1 (TRPV1) is associated with aging and lifespan, and its activation causes apoptotic cell death in various cell types. However, the effect of TRPV1 on testicular apoptosis in aged mice has not yet been reported. TRPV1 knockout (KO) mice had a longer lifespan than that of wild-type (WT) mice. Lifespan was increased by 11.8% in male TRPV1 KO mice compared to that in WT mice. TRPV1 KO males lived approximately 100 days longer than WT males on average, and the maximum lifespan was markedly extended in TRPV1 KO mice compared with that in WT mice. The TRPV1 expression levels were highly increased in the testes of older mice. TRPV1 was expressed in the entire testes region of the old mice. In addition, old TRPV1 KO mice had lower testicular apoptosis than that of WT mice. Our results show that TRPV1 induces testicular apoptosis and suggest that TRPV1 may be associated with testicular aging.

• Asian-Australasian Journal of Animal Sciences
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• 제16권11호
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• pp.1565-1571
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• 2003

Physiological mechanisms, involved in unusual ovulatory sequences in domestic hen are remaining undefined. One hundred individually caged white leghorn birds were divided into two equal groups viz. control and treatment, and 2-bromo-$\alpha$-ergocryptine, was administered to birds in the treatment group to modulate prolactin (PRL) secretion from anterior pituitary gland. The effect of modulation of PRL concentrations on egg production, sequence length and intersequence pause length were studied by analysis of oviposition records of the birds from 24 to 72 weeks of age. The surviving 48 birds in the control and treatment groups averaged $34.58{\pm}1.7$ and $25.67{\pm}1.15$ sequences of oviposition, with a mean sequence length of $9.92{\pm}0.63$ and ${\pm}1.12$ days respectively. Most of the birds had a single characteristically long sequence during the entire reproductive cycle, which averaged $46.04{\pm}3.09$ days in the control birds and $59.33{\pm}4.44$ days in the treated birds. 2-bromo-$\alpha$-ergocriptine treatments had significantly decreased (p$\leq$0.01) the circulating concentrations of PRL compared to the birds of the control group. This resulted in a significant increase (p$\leq$0.01) in the number of laying days in birds of the treatment group with a concomitant decrease in the intersequence pause length. The decreased PRL levels during prime sequences in birds of the both groups, reveals the negative role of the circulating PRL levels on egg production with concomitant shorter intersequence pause length. Hence, modulation of PRL with dopamine agonist may enhance the reproductive efficiency of hens later in life.

• The Korean Journal of Physiology
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• 제9권2호
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• pp.17-22
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• 1975

Adult nonpregnant female rabbits were subjected to the study of the effects of carbon monoxide inhalation on the uterine motility. Animals were anesthetized with intravenous injection of nembutal, 35 mg/kg, and the uteri were exposed. Polyethylene tubing which had a small hole near the blind tip was inserted in the loop and normal saline was infused at a constant rate of 1.5 ml/min. On the other end of the loop, an outlet of fluid was made. When a peristaltic wave proceeded to the hole, a rise of the pressure was ensued and it was transmitted to the pressure transducer, making an upward deflection of the recording pen on the physiograph. Carbon monoxide, 1,000 ppm in the concentration, was inhaled through a tracheal cannula for 30 minutes, following fresh air for 30 minutes. In some cases, pure oxygen was also supplemented for another 30 minutes. Uterine motility was expressed in terms of the impulse that was the time integral of the pressure and of the frequency of the peristaltic waves. The results obtained were as follows. 1. When 1,000 ppm carbon monoxide was inhaled for 30 minutes, the impulse dropped to $72{\pm}16.5%$ and the frequency to $75{\pm}22.7%$ of the values obtained before the gas administration. 2. By fresh air for 30 minutes, the impulse and the frequency restored to $77{\pm}25.7%$ and $92{\pm}21.1%$, respectively. 3. By the supplement of pure oxygen for 30 minutes, no remarkable improvement were revealed, showing $89{\pm}35.2%$ in the impulse and $91{\pm}10.8%$ in the frequency, respectively. 4. There was an appreciable discrepancy in the recovery courses of the impulse and the frequency, suggesting different mechanisms attributable to the alteration by carbon monoxide inhalation.

• Journal of Ginseng Research
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• 제36권1호
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• pp.47-54
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• 2012

Korean red ginseng (KRG) has been used worldwide as a traditional medicine for the treatment of various reproductive diseases. Gonadotropin releasing hormone (GnRH) neurons are the fundamental regulators of pulsatile release of gonadotropin required for fertility. In this study, an extract of KRG (KRGE) was applied to GnRH neurons to identify the receptors activated by KRGE. The brain slice patch clamp technique in whole cell and perforated patch was used to clarify the effect of KRGE on the membrane currents and membrane potentials of GnRH neurons. Application of KRGE (3 ${\mu}g$/${\mu}L$) under whole cell patch induced remarkable inward currents (56.17${\pm}$7.45 pA, n=25) and depolarization (12.91${\pm}$3.80 mV, n=4) in GnRH neurons under high $Cl^-$ pipette solution condition. These inward currents were not only reproducible, but also concentration dependent. In addition, inward currents and depolarization induced by KRGE persisted in the presence of the voltage gated $Na^+$ channel blocker tetrodotoxin (TTX), suggesting that the responses by KRGE were postsynaptic events. Application of KRGE under the gramicidin perforated patch induced depolarization in the presence of TTX suggesting its physiological significance on GnRH response. Further, the KRGE-induced inward currents were partially blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; non-NMDA glutamate receptor antagonist, 10 ${\mu}M$) or picrotoxin (PIC; $GABA_A$ receptor antagonist, 50 ${\mu}M$), and almost blocked by PIC and CNQX mixture. Taken together, these results suggest that KRGE contains ingredients with possible GABA and non-NMDA glutamate receptor mimetic activity, and may play an important role in the endocrine function of reproductive physiology, via activation of $GABA_A$ and non-NMDA glutamate receptors in GnRH neurons.

• 한국발생생물학회지:발생과생식
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• 제22권1호
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• pp.85-94
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• 2018

Endocrine disruptors have been concerned in toxicology but now challenged as physiological point especially concerned with exposing dose and period. In this study the low-dose chronic administration of di(2-ethylhexyl) phthaltae (DEHP) during reproductive period was examined to evaluate the possible roles. Adult male and female CD-1 mice were exposed to DEHP with drinking water containing $133{\mu}g/L$ and $1,330{\mu}g/L$ DEHP in water according to OECD 433 guide line and sacrificed just after weaning. The weights of uterus and ovary were decreased by drinking of $1,330{\mu}g/L$ DEHP water. There was not adverse effects on either accumulated mating rate and mating rate depend on estrus stage, pregnancy duration, and sex ration at birth. However, the accumulated rate of successful delivery and litter size were significantly high at $1,330{\mu}g/L$ DEHP water. The number of epididymal sperm was significantly increased by drinking of $1,330{\mu}g/L$ DEHP water. In addition, the number of follicles (primary, secondary, tertiary) were more many than control at $1,330{\mu}g/L$ DEHP water drunk mother. Though further studies are needed to identify what are the mechanism of DEHP in folliculogenesis and spermatogenesis. From this study we firstly report the effect of low-dose chronic administration of DEHP with drinking could change the ovarian follicle population size and spermatogenesis rate. Put together, those finding is different from previous high-dose effects and suggest the physiological role of DEHP in gonads and uterus.