• Title/Summary/Keyword: reproductive biology

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Reproductive Biology of the Washington Clam Saxidomus purpuratus in the Coastal Waters of Yeosu, Korea (한국 여수에 서식하는 개조개(Saxidomus purpuratus)의 산란생태)

  • Da Yeon Kang;Seong Yong Moon;Kyung Mi Jung;Gun Wook Baeck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.57 no.1
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    • pp.53-59
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    • 2024

  • The reproductive biology of the Washington clam Saxidomus purpuratus, from Yeosu, Korea was investigated based on 699 individuals collected from January to December 2022. The sex ratio of this species was not significantly different from a 1:1 (male:female) sex ratio (P>0.05). Monthly changes in gonadosomatic index were analyzed to estimate the spawning season. The reproductive cycle of this species can be divided into the following stages: active (Jan.-Mar.), ripe (Mar.-Nov.), spent (Apr.-Dec.), and degenerative (Jun.-Dec.) stages. The spawning period ranged from March to December with the main periods being from April to June and from September to November. The shell length at 50% female group maturity (SL50) was estimated to be 60.2 mm.

  • https://doi.org/10.5657/KFAS.2024.0053 인용 PDF HTML

Preimplantation genetic diagnosis for Charcot-Marie-Tooth disease

  • Lee, Hyoung-Song;Kim, Min Jee;Ko, Duck Sung;Jeon, Eun Jin;Kim, Jin Young;Kang, Inn Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.40 no.4
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    • pp.163-168
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    • 2013

  • Objective: Preimplantation genetic diagnosis (PGD) is an assisted reproductive technique for couples carrying genetic risks. Charcot-Marie-Tooth (CMT) disease is the most common hereditary neuropathy, with a prevalence rate of 1/2,500. In this study, we report on our experience with PGD cycles performed for CMT types 1A and 2F. Methods: Before clinical PGD, we assessed the amplification rate and allele drop-out (ADO) rate of multiplex fluorescent polymerase chain reaction (PCR) followed by fragment analysis or sequencing using single lymphocytes. We performed six cycles of PGD for CMT1A and one cycle for CMT2F. Results: Two duplex and two triplex protocols were developed according to the available markers for each CMT1A couple. Depending on the PCR protocols, the amplification rates and ADO rates ranged from 90.0% to 98.3% and 0.0% to 11.1%, respectively. For CMT2F, the amplification rates and ADO rates were 93.3% and 4.8%, respectively. In case of CMT1A, 60 out of 63 embryos (95.2%) were diagnosed and 13 out of 21 unaffected embryos were transferred in five cycles. Two pregnancies were achieved and three babies were delivered without any complications. In the case of CMT2F, a total of eight embryos were analyzed and diagnosed. Seven embryos were diagnosed as unaffected and four embryos were transferred, resulting in a twin pregnancy. Two healthy babies were delivered. Conclusion: This is the first report of successful pregnancy and delivery after specific PGD for CMT disease in Korea. Our PGD procedure could provide healthy babies to couples with a high risk of transmitting genetic diseases.

  • https://doi.org/10.5653/cerm.2013.40.4.163 인용 PDF KSCI

Effects of Short-Term Treatment with Estrogen Receptor Agonist on Morphological Changes in Reproductive Organs of Adult Male Mice

  • Choi, Hayana;Mo, Yun Jeong;Lee, Mi-Kyung;Choe, Eun Sang;Yee, Sung Tae;Cho, Hyun Wook
    • Applied Microscopy
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    • v.46 no.4
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    • pp.193-200
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    • 2016

  • Male reproductive organs are strongly affected by estrogen signaling mediated via the estrogen receptor. In this study, propyl pyrazole triol (PPT), an estrogen receptor alpha agonist, was subcutaneously injected in adult male mice every 2 days for a total duration of 8, 16, or 24 days. Histological changes in the reproductive organs, including the testes, efferent ductules, and epididymides, were observed. The weight of the reproductive organs decreased in the PPT group. In addition, the diameter of the seminiferous tubules decreased in the PPT group compared with the control group. The epithelial cell height decreased in the initial segment of the epididymis, whereas the luminal diameter increased in the efferent ductules of the PPT group. PPT induced irregular morphology of stereocilia in the luminal region of the initial segment. Therefore, PPT treatment at high concentrations had inhibitory effects in the reproductive organs of adult male mice. These findings suggest that short-term treatment with estrogen receptor agonist causes histological changes in the testes, efferent ductules, and epididymis, which are similar to those caused by estrogen receptor antagonist treatment. Therefore, the estrogen receptor may have functional roles in male reproductive organs, implying that treatment with an estrogen receptor agonist can affect male fertility.

  • https://doi.org/10.9729/AM.2016.46.4.193 인용 PDF KSCI