• KSBB Journal
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• v.15 no.4
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• pp.393-397
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• 2000

The cometaboic biodegradation conditionso f trichloroethylene(TCE) by Burkholderia cepacia G4 were optimized using response surface analysis. The experimental sets of phenol concentration temperature and pH were designed using central composite experimental design. The optimal conditions of phenol concentration temperature and pH were determined to be 0.91 ppm 21.5$^{\circ}C$ and 7.65 respectively by the Ridge analysis of the contour plot for TCE biodegradation rates. The TCE biodegradation rate could be enhanced up to 2.43 nmol.mg protein$.$min by response surface methodology.

• Journal of Biomedical Engineering Research
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• v.3 no.2
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• pp.95-100
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• 1982

Glucose oxidase from A. niger was entrapped in polyacrylamide gel which was used in the enzyme electrode for glucose analysis. The electrode was assembled by placing the gel between the membranes on the surface of a Clark type electrode. In order to make it possible to analyze the experimental results later, the stagnation flow was adopted wheree the governing fluid mechanics were well known. The current increased with the increase concentration in the bulk below a certain level of glucose concentration beyond which no more current increase was observed. This is probably due to the diffusion limitation of oxygen from the bulk solution. Also the current increased witll the enzyme loading in the gel, but the linearity between the current and the glucose concentration was rather limited to a narrow range. Flow rate was found to be very important, which means that film diffusion is very important under the flow rate of 5cm/sec. As a conclusion, enzyme loading, gel layer thickness, stirring speed and bulk concentration of glucose were found to be most improtant parameters in yielding a linar current reponse with respect to the bulk glucose concentration.

• The Journal of Engineering Geology
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• v.1 no.1
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• pp.109-120
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• 1991

Applications of microtremor to geological engineering are widely reviewed and observed microtremors are processed to show evidences of close relationship between the predominant periods of microtremor and underground structure. The ground vibrates continuously at all times and the elastic vibration is called microtremor (0.5-20Hz) or microseisms (0.01-0.1Hz) according to their frequency range. The vibration is believed to have propagative nature like those of the dispersive surface waves or multireflected shear waves. Microtremors were recorded at selected thress places of which subsoil structures are well distinguished in the Kyongsang Sedimentary Basin. It is found that the underground structures estimated from microtremor analysis coincide well with the known structures. The microtremor analysis of the long period range can be an inexpensive and effective tool in geological engineering for the evaluation of the underground structure, site-specific reponse spectrum, and seismic microzonations.