• 한국수의병리학회지
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• 제3권1호
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• pp.1-5
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• 1999

This experiment was carried out to establish the immunohistochemical diagnostic method for infectious pancreatic necrosis in the monolayers of CHSE-214 cell cultures and paraffin-embedded tissue sections from rainbow trout infected with infectious pancreatic necrosis virus(IPNV). Specific identification of IPNV antigens was often demonstrated in the pancreatic exocrine cells, and less in the intestinal mucous epithelia and the renal hemopoietic tissues by the use of monoclonal antibodies against capsid protein VP2. The specific reaction was seen as a distinct red cytoplasmic color, often as small granules of various sizes. The result showed that streptavidin alkaline phosphatase immunohistochemisry specifically identified IPNV antigens in both infected cell cultures and tissue sections.

• 대한세포병리학회지
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• 제5권2호
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• pp.160-166
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• 1994

Collecting duct carcinoma of the kidney is an unusual variety of renal carcinoma considered to arise from the epithelium of the collecting ducts. We recently experienced a case of fine needle aspiration cytology of collecting duct carcinoma of the kidney in a 17 year-old girl. The smear revealed many cellular clusters of ordinary papillary pattern, characterized by clumping of cells with nuclear overlapping, in a slightly necrotic background. The tumor cells had abundant delicate granular cytoplasm with some having vacuolation. The nuclei were only slightly pleomorphic with somewhat coarse chromatin and one or more small nucleoli Some nuclei showed irregular nuclear membrane and nuclear groove. A few polymorphs were also present.

• Imaging Science in Dentistry
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• 제51권1호
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• pp.87-90
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• 2021

The increased use of cone-beam computed tomographic (CBCT) scans has made it increasingly necessary to evaluate incidental findings on CBCT scans. This report describes the case of a 66-year-old female patient who presented to the Department of Oral and Maxillofacial Pathology, Radiology and Medicine at the College of Dentistry of the author's institution and underwent a CBCT scan for maxillary alveolar process implant planning. Upon evaluation of the CBCT scan, a radiopaque (soft tissue attenuation) mass in the left superior aspect of the nasal cavity and left locule of the sphenoid sinus with opacification of the left locule of the sphenoid sinus was incidentally noted. These radiographic findings were suggestive of a space-occupying mass with a high possibility of malignancy. A further medical evaluation confirmed renal cell cancer metastasis to the sphenoid sinus. This study shows the significance of reviewing the entire CBCT scan for incidental findings.

• Childhood Kidney Diseases
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• 제5권2호
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• pp.125-135
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• 2001

목 적 : 국소성 분절성 사구체 경화증 환자에서 장기예후를 예측하기 위한 많은 노력들이 있어 왔으나 현재까지 정립된 의견은 없는 상태이며, 특히 소아환자인 경우는 보고된 논문이 별로 없는 상태이다. 이에 저자들은 그동안 경험한 국소성 분절성 사구체 경화증 환아들에서 장기예후에 영향을 줄수 있는 인자들을 알아보고자 하였다. 대상 및 방법 : 1990년 1월부터 1999년 12월까지 연세 의료원에서 신생검을 시행하여 병리 소견상 국소성 분절성 사구체 경화증으로 확진된 환아 50명에서 후향적 방법으로 자료 분석을 시행하였다. 진단 당시 임상검사소견, 치료에 대한 반응 및 병리소견을 바탕으로 신기능 유지군과 신기능 저하군을 비교하고 신생존율을 알아보았으며 신부전으로 진행되는 속도에 영항을 미치는 인자도 검토하였다. 결 과 : 평균 발병연령은 8년 1개월이며 남녀 성비는 2.3 : 1이었으며 평균 추적 관찰 기간은 7년 1개월이었다. 전체 50명에서의 신생존율은 5년에 $34\%$, 10년에 $8\%$이었고 신기능유지군의 5년 신생존율은 $74\%$이었고 신기능저하군은 $27\%$이었다. 신기능유지군과 신기능저하군을 비교했을 때 발병연령, 성별, 단백뇨량, 혈뇨유무, 고혈압유무, 조직학적으로 메산지움 증식유무는 차이가 없었으며 신기능저하군에서 진단 당시 혈중 크레아티닌치가 높았고 치료반응이 불량했으며 사구체 경화증 침범정도 및 세뇨관 간질 변화가 많았고 혈관변화를 동반하였다(P<0.05). 5년 신생존율은 진단 당시 크레아티닌치가 높았던 경우, 고혈압이 있었던 경우, 치료반응이 불량한 경우, 사구체 경화증 침범정도와 세뇨관 간질변화가 많았던 경우, 혈관 변화가 동반된 경우에 통계학적으로 유의하게 낮은것으로 나왔다(P<0.05). 신부전으로의 진행속도는 통계적으로 유의한 예측인자는 없었으나 발병연령이 어릴때, 고혈압이 있을 때, 치료반응이 나쁠 때 그리고 신생검 상 혈관 변화가 동반될때 급속히 진행하는 것을 알 수 있었다(P>0.05). 결 론 : 이상의 고찰에서 소아의 국소성 분절성 사구체 경화증의 장기예후에 영향을 미치는 인자를 알아 보았으며 이러한 예후인자를 미리 예측함으로써 치료 방법을 세우고 결과를 예측하는 데 도움이 되리라 생각한다.

• 동의생리병리학회지
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• 제21권5호
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• pp.1185-1193
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• 2007

Ginsing polysaccharide, known to have an immune regulating effect, was administered to 23 randomly selected healthy male subjects with a mean age of 23 years in accordance with an IRB approval. Then, these subjects underwent physico-chemical tests and serum proteome was analyzed from the blood sample taken from these subjects. Analyses of proteome involved image analysis, protein sections and protein identification in sequence after two-dimensional electrophoresis was carried out. During the physico-chemical test, 4 subjects were excluded from the study. In the proteome analysis, identified were 5 spots such as SP40, 40, Cytokeratin 9, hypothetical protein LOC544932, Apolipoprotein E ,similar to Human albumin, which showed differences in the amount of protein expression. In conclusion, changes of 5 proteins were remarkable before and after administration of ginsing polysaccharides. In certain cases, hepatic and renal slight injury occurred. Thus, further clinical study on dosage regimen would be necessary for securing the basis for concentration-dependent effectiveness and safety.

• Childhood Kidney Diseases
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• 제6권1호
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• pp.123-130
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• 2002

이식후 발생하는 림프증식성 질환(PTLD)은 양성 다크론성 B림프구 증식증으로부터 악성의 단크론 림프종에 이르기까끼 다양한 임상상을 보이는 질환이다. PTLD는 Epstein-Barr virus(EBV)와 과도한 면역억제와 밀접한 관계가 있다. 국내에서는 1997년 성인에서 신 이식 후 발생한 예가 처음 보고되었으며, 소아에서는 간 이식 후 발생한 예가 있을 뿐 신 이식 후 발생한 증례의 보고는 없어 이 보고가 국내 최초로 신이식 소아에서 발생한 PTLD 증례이다. 환자는 9세 남아로서 긴이식후 4개월후에 PTLD가 발생하였으며 주된 임상 양상은 발열, 림프절 종대와 혈변이었다. 림프절과 대장의 생검조직 에서 EBV in situ hybridization이 양성이고, 조직학적으로 B 세포계열의 PTLD에 합당한 소견이었다. 모든 증상은 면역억제제 감소와 ganciclovir 치료로 완전 관해되었다.

• The Korean Journal of Physiology and Pharmacology
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• 제20권4호
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• pp.333-340
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• 2016

Edaravone, a synthetic-free radical scavenger, has been reported to reduce ischemia-reperfusion-induced renal injury by improving tubular cell function, and lowering serum creatinine and renal vascular resistance. The present study investigated the effect of edaravone in diabetes mellitus-induced nephropathy in rats. A single administration of streptozotocin (STZ, 55 mg/kg, i .p.) was employed to induce diabetes mellitus in rats. The STZ-administered diabetic rats were allowed for 10 weeks to develop nephropathy. Mean body weight, lipid alteration, renal functional and histopathology were analysed. Diabetic rats developed nephropathy as evidenced by a significant increase in serum creatinine and urea, and marked renal histopathological abnormalities like glomerulosclerosis and tubular cell degeneration. The kidney weight to body weight ratio was increased. Moreover, diabetic rats showed lipid alteration as evidenced by a significant increase in serum triglycerides and decrease in serum high-density lipoproteins. Edaravone (10 mg/kg, i .p., last 4-weeks) treatment markedly prevented the development of nephropathy in diabetic rats by reducing serum creatinine and urea and preventing renal structural abnormalities. In addition, its treatment, without significantly altering the elevated glucose level in diabetic rats, prevented diabetes mellitus-induced lipid alteration by reducing serum triglycerides and increasing serum high-density lipoproteins. Interestingly, the renoprotective effect of edaravone was comparable to that of lisinopril (5 mg/kg, p.o, 4 weeks, standard drug). Edaravone prevented renal structural and functional abnormalities and lipid alteration associated with experimental diabetes mellitus. Edaravone has a potential to prevent nephropathy without showing an anti-diabetic action, implicating its direct renoprotection in diabetic rats.

• Korean Journal of Radiology
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• 제22권12호
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• pp.1996-2005
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• 2021

Objective: To investigate the multidetector computed tomography (MDCT) features of fumarate hydratase-deficient renal cell carcinoma (FH-deficient RCC) with germline or somatic mutations, and compare them with those of papillary type II RCC (pRCC type II). Materials and Methods: A total of 24 patients (mean ± standard deviation, 40.4 ± 14.7 years) with pathologically confirmed FH-deficient RCC (15 with germline and 9 with somatic mutations) and 54 patients (58.6 ± 12.6 years) with pRCC type II were enrolled. The MDCT features were retrospectively reviewed and compared between the two entities and mutation subgroups, and were correlated with the clinicopathological findings. Results: All the lesions were unilateral and single. Compared with pRCC type II, FH-deficient RCC was more prevalent among younger patients (40.4 ± 14.7 vs. 58.6 ± 12.6, p < 0.001) and tended to be larger (8.1 ± 4.1 vs. 5.4 ± 3.2, p = 0.002). Cystic solid patterns were more common in FH-deficient RCC (20/24 vs. 16/54, p < 0.001), with 16 of the 20 (80.0%) cystic solid tumors having showed typical polycystic and thin smooth walls and/or septa, with an eccentric solid component. Lymph node (16/24 vs. 16/54, p = 0.003) and distant (11/24 vs. 3/54, p < 0.001) metastases were more frequent in FH-deficient RCC. FH-deficient RCC and pRCC type II showed similar attenuation in the unenhanced phase. The attenuation in the corticomedullary phase (CMP) (76.3% ± 25.0% vs. 60.2 ± 23.6, p = 0.008) and nephrographic phase (NP) (87.7 ± 20.5, vs. 71.2 ± 23.9, p = 0.004), absolute enhancement in CMP (39.0 ± 24.8 vs. 27.1 ± 22.7, p = 0.001) and NP (50.5 ± 20.5 vs. 38.2 ± 21.9, p = 0.001), and relative enhancement ratio to the renal cortex in CMP (0.35 ± 0.26 vs. 0.24 ± 0.19, p = 0.001) and NP (0.43 ± 0.24 vs. 0.29 ± 0.19, p < 0.001) were significantly higher in FH-deficient RCC. No significant difference was found between the FH germline and somatic mutation subgroups in any of the parameters. Conclusion: The MDCT features of FH-deficient RCC were different from those of pRCC type II, whereas there was no statistical difference between the germline and somatic mutation subgroups. A kidney mass with a cystic solid pattern and metastatic tendency, especially in young patients, should be considered for FH-deficient RCC.

• Childhood Kidney Diseases
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• 제8권2호
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• pp.149-158
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• 2004

목적: 저자들은 소아 IgA 신병증 환자를 대상으로 세뇨관 상피세포에서 ICAM의 발현이 신 손상의 예후인자로서 역할을 할 수 있는지를 알아보기 위하여, 신세뇨관 ICAM-1 발현과 조직소견 및 임상소견과의 연관성을 조사하고자 본 연구를 시행하였다. 방법: IgA 신병증으로 진단된 43명의 환자를 후향적으로 분석하였고, Haas가 제안한 분류법에 따라 subclasses에 따라 비교하였다. 그리고 LSAB kit를 이용하여 세뇨관 상피 세포의 ICAM-1의 발현도를 평가했다. 결과: IgA 신병증으로 진단된 43명의 평균 연령은 $12.2{\pm}2.2$세였으며, 남자 28명, 여자 15명이었다. 육안적 혈뇨에 따른 신세뇨관 ICAM-1 발현의 차이는 발견할 수 없었다. 그러나 단백뇨가 동반되었던 환자의 신세뇨관 ICAM-1의 발현율은 단백뇨가 없었던 환자들의 발현율에 비해 통계적으로 유의하게 높았다$78.2{\pm}14.19%\;vs\;55.8{\pm}32.20%,\;P<0.05$. Haas의 조직학적 분류의 subclasses에 따른 ICAM-1의 발현율은 subclass가 증가할수록 ICAM-1의 발현율도 증가하였다. 신세뇨관 ICAM-1의 발현과 24시간 채집뇨의 총단백량 그리고 신세뇨관 ICAM-1의 발현과 조직학적 분류간의 상관성은 Spearmann 상관분석상 각각 통계적으로 유의한 상관관계를 보였다. 그러나 간질 염증세포 침윤, 섬유화, 신세뇨관 위축 정도와는 통계학적으로 유의한 상관관계를 보이지 않았다. 결론: 소아 특발성 IgA 신병증에서 신세뇨관 ICAM-1의 발현은 신 손상 특히 사구체 손상의 정도를 잘 반영하고 있으며 예후 인자로서도 유용하게 이용할 수 있다고 생각한다.

• 한국수의병리학회지
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• 제1권1호
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• pp.66-71
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• 1997

Eighteen NZW rabbits used for local skin irritation study were examined grossly and microscopically for natural infection with Encephalitozoon cuniculi. For microscopic tissue evaluation histochemical techniques such as PAS Gram iron hematoxylin and HE stain were used. Although rabbits in the study had no abnormal clinical signs 7/18(38.9%) animals were microscopically infected with E. cuniculi. The affected rabbits had gray-whitish and depressed approximately 0.1∼0.6 cm diameter lesions in the kidneys. All other organs examined were grossly unremarkable. Histopathologically however in addition to segmental interstitial nephritis focal lymphocytic myocarditis and granulomatous inflammatory reaction in portal areas of the liver multifocal granulomatous foci with vasculitis were present in the brain kidney and lungs. Aggregates of minute oval organisms were observed in brain and kidney sections frequently within the granulomatous foci and sometimes without any inflammatory reaction particularly in the renal tubules. in histochemical stains the organisms were gram positive stained with iron hematoxylin and had PAS positive granule at one pole. They measured approximately 1.5×2.5μm consistent with E. cuniculi. Histochemical characterization is important to differentiate E. cuniculi from other common protozoal infection such as Toxoplasma gondii. This study demonstrate the importance of subclinical. E. cunuculi infection and the associated histological alterations may interfere with tissue evaluation in toxicologic studies.