• Journal of Bio-Environment Control
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• v.12 no.2
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• pp.72-76
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• 2003

This study was conducted to estimate stimulation of plant growth of green and red leafy lettuces (Lactuca sutiva L. cv. Chungchima and Jugchima) at affected by covering method and time with protected film materials containing concentrated PO film and GA$_3$ foliar spray. Plants were covered with ‘Pasraido’ material from October 26 to November 31 (direct 1), from November 5 to November 31 (direct 2), or with a tunnel, or direct 2＋ tunnel. Mean air temperature and relative humidity under the covering were higher in direct and tunnel, direct and non-covering, in that order. The fresh weight and dry weight of red and green lettuces increased in direct and tunnel followed by direct 1, direct 2, tunnel and the control. GA$_3$ treatment promoted the growth in all covering methods, but decreased the chlorophyll contents. Fresh weight and dry weight were higher in red lettuce than in green lettuce in the control.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.234-239
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• 2010

The irrigation interval and time for the supply of nutrient and water to the plant is important in the aeroponic system. This study was conducted to investigate the effect of irrigation interval on the growth of Phalaenopsis hybrid seedlings in the aeroponic system. Young bare-root plants (four leaves, 16 g in fresh weight) were used for this experiment. The composition of nutrient solution was, in $me{\cdot}L^{-1}$, 9 N, 3 P, 4 K, 4 Ca and 2 Mg. The electrical conductivity (EC) and pH of nutrient solution used was $1.2\;dS{\cdot}m^{-1}$ and 5.8, respectively. Irrigation intervals were 10, 20, 30, 40, and 50 minute and each irrigation time was 10 minute. The total fresh and dry weight, the number of branched roots, and relative growth rate at the 20 and 30 min. was greater than 10, 40, 50 min. interval. Especially, the fresh weight of roots at 30 min. interval was the highest. Leaf length was the highest at 30 min. interval but there was no difference in leaf width. The amount of water consumed for a month was 0.71 L per plant and it was reduced with increasing irrigation interval. There was no difference in the amount of consumed mineral contents for 15 days except for potassium. Potassium absorbed was the highest at 30 min. irrigation interval. As a result, the optimum irrigation interval was 30 min for the production of Phalaenopsis hybrid seedlings in the aeroponic system.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.8
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• pp.1154-1161
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• 2000

Two experiments were conducted to evaluate the efficacy of cupric citrate (Cu-citrate) relative to cupric sulfate $(CuSO_4)$ as a Cu source for weanling and grow-finish pigs. In addition, the use of liver and bile Cu concentrations as indices of the bioavailability of Cu sources was investigated. Experiment one consisted of a nursery phase (35 d; initial BW=6.4 kg, final BW=21.4 kg) followed by a grow-finish phase (103 d; initial BW=21.5 kg, final BW=111.7 kg). Experiment two only consisted of a nursery phase (35 d; initial BW=6.3 kg, final BW=18.6 kg). Dietary treatments were identical for both experiments and consisted of: control (10 ppm $CuSO_4$); control+66 or 225 ppm $CuSO_4$; control+33, 66, or 100 ppm Cu-citrate. An antibiotic was included in diets for Exp. 1 but not Exp. 2. In both experiments, growth performance variables were similar for pigs receiving Cu-citrate and $CuSO_4$; however, growth performance was not improved by high concentrations of $CuSO_4$. Liver and bile Cu were increased (p<0.05) by 225 ppm $CuSO_4$; however, lower dietary concentrations of Cu from either $CuSO_4$ or Cu-citrate did not affect the Cu concentration of liver or bile relative to that observed in the control pigs. Irrespective of Cu source, there was no linear (p>0.10) increase in plasma Cu with increasing Cu concentrations in the diet for both experiments. However, the plasma Cu concentrations were highest (p<0.10) in pigs receiving diets supplemented with 225 ppm $CuSO_4$. Sixteen randomly chosen pigs per treatment in Exp. 1 were continued through the grow-finish phase. Body weight gain and feed intake were improved (p<0.10) by 66 ppm $CuSO_4$, but other dietary Cu treatments did not alter pig performance compared to the control diet. Plasma Cu concentrations were increased (p<0.10) by 225 ppm $CuSO_4$ in the growing phase and by 225 ppm $CuSO_4$ and 100 ppm Cu-citrate in the finishing phase. These data reveal no consistent effect of $CuSO_4$ on performance; therefore, it is difficult to assess the efficacy of these two Cu sources. In addition, these studies demonstrate that liver and bile Cu are not good indicators of Cu bioavailability in pigs fed adequate to pharmacological concentrations of Cu.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.12
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• pp.1732-1741
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• 2013

Two experiments were conducted to determine physical and chemical properties of vermicelli waste (VW) and effect of VW inclusion levels on growth performance of broilers. In experiment 1, VW samples were randomly collected from vermicelli industry in Thailand to analyze nutritional composition. Vermicelli waste contained 9.96% moisture, 12.06% CP, 32.30% crude fiber (CF), and 0.57% ether extract (EE), as DM basis. The ratio of insoluble:soluble non-starch polysaccharide (NSP) was 43.4:8.9. A total of 120 chicks (6 pens per treatment and 10 chicks per pen) were fed a corn-soybean meal-based diet or 20% VW substituted diet to determine the apparent metabolizable energy corrected for nitrogen retention ($AME_n$) of VW. The $AME_n$ of VW was $1,844.7{\pm}130.71$ kcal/kg. In experiment 2, a total of 1,200 chicks were randomly allotted to 1 of 4 dietary treatments for 42-d growth assay. There were 300 chicks with 6 pens per treatment and 50 chicks per pen. The dietary treatments contained 0%, 5%, 10%, or 15% VW, respectively. All diets were formulated to be isocaloric and isonitrogenous. From 0 to 18 d of age chicks fed VW diets had higher (p<0.001) feed conversion ratio (FCR) compared with those fed the control diet. No difference was observed during grower and finisher phase (19 to 42 d). Chicks fed VW diets had lower relative weight of abdominal fat (p<0.001) but higher relative weight of gizzard (p<0.05) than those of chicks fed the control diet. Increasing VW inclusion levels increased ileal digesta viscosity (p<0.05) and intestinal villus height of chicks (p<0.001). For apparent total tract digestibility assay, there were 4 metabolic cages of 6 chicks that were fed experimental treatment diets (the same as in the growth assay) in a 10-d total excreta collection. Increasing VW inclusion levels linearly decreased (p<0.05) apparent total tract digestibility of DM and CF.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.4
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• pp.544-550
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• 2015

This study was conducted to investigate effects of heat-oxidized soy protein isolate (HSPI) on growth performance, serum biochemical indices, apparent nutrient digestibility and digestive function of broiler chickens. A total of 320 1-day-old Arbor Acres chicks were randomly divided into 4 groups with 8 replicates of 10 birds, receiving diets containing soy protein isolate (SPI, control group) or the same amount of SPI heated in an oven at $100^{\circ}C$ for 1, 4, or 8 h, for 21 days, respectively. The results indicated that compared with the control group, body weight gain and feed intake of birds fed diet containing SPI heated for 8 h were significantly lower (p<0.05). Serum urea nitrogen concentration was higher in the broilers fed diet containing SPI heated for 4 or 8 h at d 21 (p<0.05). In contrast, serum glucose content was decreased by HSPI substitution at d 21 (p<0.05). The relative pancreas weight in HSPI groups was higher than that in the control group at d 21 (p<0.05). Meanwhile, the opposite effect was observed for relative weight of anterior intestine and ileum in broilers fed a diet containing SPI heated for 8 h (p<0.05). Birds fed diets containing SPI heated for 4 or 8 h had a decreased lipase activity in anterior intestinal content at d 14 and 21 (p<0.05), respectively. In addition, the same effect was also noted in broilers given diets containing SPI heated for 1 h at d 21 (p<0.05). Similarly, amylase, protease and trypsin activity in anterior intestinal content were lower in broilers fed diets containing SPI heated for 8 h at d 21 (p<0.05). The apparent digestibility of dry matter (DM) from d 8 to 10 and DM, crude protein (CP), and ether extract from d 15 to 17 were lower in broilers fed diets containing SPI heated for 8 h (p<0.05). Besides, birds given diets containing SPI heated for 4 h also exhibited lower CP apparent digestibility from d 15 to 17 (p<0.05). It was concluded that HSPI inclusion can exert a negative influence on the growth performance of broilers, which was likely to result from the simultaneously compromised digestive function.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.574-580
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• 2019

In this paper, the economic performance of the Jeju Free International City and the Free Economic Zone is investigated using statistical testing and the difference in differences (DID) model with data on foreign direct investment (FDI), gross regional domestic product (GRDP), and employment-to-population ratio (EPR). The relationships among FDI, GRDP, and EPR are also investigated using the panel vector error-correction model on the regional data. The compound average growth rate of actual investment, and the ratio of FDI received to FDI declared in the capital region were higher than in the non-capital region. For the growth and relative volume of FDI received, seven regions out of 16 were found to be low in growth and small in relative volume. The results of statistical testing showed statistically significant differences in some variables, except for two regions, but DID estimates that determine the pure policy effect of zone designation showed statistical insignificance. On the other hand, the explanatory power among the three variables was found to be quite limited, but it was greater in the cities, provinces, and non-capital region. In summary, it is necessary to establish the FDI inducement mechanism so the inflow of FDI can increase GRDP and EPR.

• Journal of the Korean Wood Science and Technology
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• v.36 no.4
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• pp.11-18
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• 2008

Anatomical characteristics of volumetric composition, fiber length, vessel diameter, and crystalline properties of cellulose in kenaf (Hibiscus cannabinus L.) planted in the reclaimed land of Buan-si, Korea were examined to understand the growth characteristics using a light microscopy and an X-ray diffraction method. The samples of kenaf were taken from six positions (3 cm, 35 cm, 70 cm, 105 cm, 280 cm, and 320 cm) of each stem over the growth period (July, August, September, and October) after seeding in the mid-May. In the kenaf stem, phloem constituted 10 to 15 %, xylem 66 to 82%, and pith 7 to 19%. The ray, bast fiber, and remainder comprised 50%, 20%, and 30% of the phloem, respectively. The volume of vessel, ray, and fiber in the xylem was approximately 10, 15, and 75%, respectively. The proportion of cell wall was 30.92% at the base of stem and 46.40% at the top of stem, respectively. The average length of bast fiber and xylem fiber was about 2.8 mm and 0.9 mm, respectively. Radial and tangential diameters of vessel increased with the increase of growth period, while they decreased with increasing the stem height. Relative crystallinity ranged from 70 to 79% in phloem and from 50 to 56% in xylem. Cellulose crystallite width was about 3 nm both in the phloem and xylem. Thus, the volumetric composition and cell dimensions in the phloem and xylem appeared to be varied with the growth period and the stem height.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.324-331
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• 2009

MK1 strain, an obligate aerobic heterotrophic bacterium isolated from the rotten tissue of Neungee mushroom (Sarcodon aspratus), produces a copious amount of exopolysaccharide (EPS), which could evoke macrophage activation. Investigations on optimal culture conditions of MK1 and physical properties of MK1 EPS were made. Glucose, galactose, fructose, and sucrose supported well growth of MK1, but potato starch and dextrin did not. However, lactose seemed to be a less favorable carbon source. Optimal growth of MK1 was obtained at pH 7.0, $30^{\circ}C$, and 200 rpm with 2% glucose, and 0.2~0.05% $(NH_4)_2SO_4$. $EPS_{opt}$ obtained from an optimal growth condition constituted of carbon (37.1%), nitrogen (2.2%), oxygen (49.3%), and hydrogen (6.4%), but no sulfur. Paper chrogromatogram of the acid-hydrolysate of $EPS_{opt}$ suggested that MK1 EPS seemed to be hetropolysaccharide composed of a few number of monosaccharides including amino- and acidic-sugars. Its molecular mass determined by SDS-polyacrylamide gel electrophoresis varied from 14.8 to 47.9 kDa. Physical properties of $EPS_{glu}$ obtained from cell grown in glucose medium, such as relative viscosity ($_{rel}$) and crystalline morphology were rather affected by pH of the growth medium. Relative viscosity ($_{rel}$) of exopolysaccaride (0.1 g/ml) harvested from cells grown at medium pH ranging from 6.0 and 7.5 was 1.23 and 1.39, respectively. The freeze-dried exopolysaccharide obtained at low pH (6.0 and 6.5) was fine crystaloid and water-soluble, whereas those obtained at high pH (7.0 and 7.5) was rather gluey and less water-soluble.

• Journal of agriculture & life science
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• v.45 no.3
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• pp.43-51
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• 2011

This project was conducted to measure actual temperature, relative humidity (RH), and light intensity at different apartment floors and to suggest suitable indoor plants by investigating morphological changes of Treophelosparmum asiatioum and Fittonia verchaffeltii var. argyroneura as affected by light intensity. Temperature and RH in apartment verandas were measured in three different (2nd, 9th, and 16th) floors on three different buildings for 30 days seasonally. The light intensity, temperature, and RH were recorded outside (parking area) and inside apartment verandas for 24 hours on a selected sunny summer day (between Aug. 19 and Sept. 14, 2008). Based on the first study, we investigated effect of simulated light intensity (40, 70, 100, and $600{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) on changes in growth and development of T. asiatioum and F. verchaffeltii var. argyroneurain growth chambers. However, daily mean light intensity of 2nd, 9th, and 16th floors was different each other as it was about 40, 70, and $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. In the growth chamber experiment, plant height, internode length, and leaf length and width were not affected significantly by light intensity, but changes in leaf color were apparent in the new leaves with the increasing light intensity. The results suggest that T. asiatioum could be best fit to high, and F. verchaffeltii var. argyroneura to lower floor verandas, considering their aesthetic values.

• Molecules and Cells
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• v.44 no.10
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• pp.770-779
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• 2021

Transgenic Arabidopsis thaliana expressing an anti-rabies monoclonal antibody (mAb), SO57, was obtained using Agrobacterium-mediated floral dip transformation. The endoplasmic reticulum (ER) retention signal Lys-Asp-Glu-Leu (KDEL) was tagged to the C-terminus of the anti-rabies mAb heavy chain to localize the mAb to the ER and enhance its accumulation. When the inaccurately folded proteins accumulated in the ER exceed its storage capacity, it results in stress that can affect plant development and growth. We generated T₁ transformants and obtained homozygous T₃ seeds from transgenic Arabidopsis to investigate the effect of KDEL on plant growth. The germination rate did not significantly differ between plants expressing mAb SO57 without KDEL (SO plant) and mAb SO57 with KDEL (SOK plant). The primary roots of SOK agar media grown plants were slightly shorter than those of SO plants. Transcriptomic analysis showed that expression of all 11 ER stress-related genes were not significantly changed in SOK plants relative to SO plants. SOK plants showed approximately three-fold higher mAb expression levels than those of SO plants. Consequently, the purified mAb amount per unit of SOK plant biomass was approximately three times higher than that of SO plants. A neutralization assay revealed that both plants exhibited efficient rapid fluorescent focus inhibition test values against the rabies virus relative to commercially available human rabies immunoglobulins. KDEL did not upregulate ER stress-related genes; therefore, the enhanced production of the mAb did not affect plant growth. Thus, KDEL fusion is recommended for enhancing mAb production in plant systems.