• Molecules and Cells
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• 제40권9호
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• pp.621-631
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• 2017

Vaccinia-related kinase 1 (VRK1) and VRK3 are members of the VRK family of serine/threonine kinases and are principally localized in the nucleus. Despite the crucial roles of VRK1/VRK3 in physiology and disease, the molecular and functional interactions of VRK1/VRK3 are poorly understood. Here, we identified over 200 unreported VRK1/VRK3-interacting candidate proteins by affinity purification and LC-MS/MS. The networks of VRK1 and VRK3 interactomes were found to be associated with important biological processes such as the cell cycle, DNA repair, chromatin assembly, and RNA processing. Interactions of interacting proteins with VRK1/VRK3 were confirmed by biochemical assays. We also found that phosphorylations of XRCC5 were regulated by both VRK1/VRK3, and that of CCNB1 was regulated by VRK3. In liver cancer cells and tissues, VRK1/VRK3 were highly upregulated and its depletion affected cell cycle progression in the different phases. VRK3 seemed to affect S phase progression and G2 or M phase entry and exit, whereas VRK1 affects G1/S transition in the liver cancer, which could be explained by different interacting candidate proteins. Thus, this study not only provides a resource for investigating the unidentified functions of VRK1/VRK3, but also an insight into the regulatory roles of VRK1/VRK3 in biological processes.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6375-6378
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• 2012

Background: Small-cell lung cancer (also known as SCLC) is an aggressive form and untreated patients generally die within about 3 months. To obtain further insight into mechanism underlying malignancy with this cancer, an miRNA synergistic regulatory network was constructed and analyzed in the present study. Method: A miRNA microarray dataset was downloaded from the NCBI GEO database (GSE27435). A total of 546 miRNAs were identified to be expressed in SCLC cells. Then a miRNA synergistic network was constructed, and the included miRNAs mapped to the network. Topology analysis was also performed to analyze the properties of the synergistic network. Consequently, we could identified constitutive modules. Further, common target genes of each module were identified with CFinder. Finally, enrichment analysis was performed for target genes. Results: In this study, a miRNA synergistic network with 464 miRNAs and 2981 edges was constructed. According to the topology analysis, the topological properties between the networks constructed by LC related miRNAs and LC unrelated miRNAs were significantly different. Moreover, a module cilque0 could be identified in our network using CFinder. The module included three miRNAs (hsa-let-7c, hsa-let-7b and hsa-let-7d). In addition, several genes were found which were predicted to be common targets of cilque0. The enrichment analysis demonstrated that these target genes were enriched in MAPK signaling pathways. Conclusions: Although limitations exist in the current data, the results uncovered here are important for understanding the key roles of miRNAs in SCLC. However, further validation is required since our results were based on microarray data derived from a small sample size.

• Molecules and Cells
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• 제43권6호
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• pp.551-571
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• 2020

Nuclear receptor-related 1 (Nurr1) protein has been identified as an obligatory transcription factor in midbrain dopaminergic neurogenesis, but the global set of human NURR1 target genes remains unexplored. Here, we identified direct gene targets of NURR1 by analyzing genome-wide differential expression of NURR1 together with NURR1 consensus sites in three human neural stem cell (hNSC) lines. Microarray data were validated by quantitative PCR in hNSCs and mouse embryonic brains and through comparison to published human data, including genome-wide association study hits and the BioGPS gene expression atlas. Our analysis identified ~40 NURR1 direct target genes, many of them involved in essential protein modules such as synapse formation, neuronal cell migration during brain development, and cell cycle progression and DNA replication. Specifically, expression of genes related to synapse formation and neuronal cell migration correlated tightly with NURR1 expression, whereas cell cycle progression correlated negatively with it, precisely recapitulating midbrain dopaminergic development. Overall, this systematic examination of NURR1-controlled regulatory networks provides important insights into this protein's biological functions in dopamine-based neurogenesis.

• 한국조명전기설비학회:학술대회논문집
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• 한국조명전기설비학회 2008년도 추계학술대회 논문집
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• pp.229-234
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• 2008

The new millennium has started with several innovations driven by fast evolution of the technologies in energy sector. A strong impulse towards the diffusion of new economical efficient technologies regulatory incentives related to energy production from renewable source and a small scale building trigeneration and to promotion of more sustainable environmental-friendly generation solutions, the evolution of electricity markets, more and more binding local emission constraints, and the need for improving the security of supply to reduce the energy system vulnerability. The 24 percentage energy quantify of total energy consumption consumes in commercial buildings and residential houses and the 30% portion of total $CO_2$ emissions covers also in the commercial buildings and residential houses sector. To cope with efficiently this energy sinuation in building sector, Building microgrid or building tooling, heating & power(BCHP) system has been interested in recent day due to meeting thermal and electric energy requirements efficiently and with appropriate energy quality. A multi agent system is a collective of intelligent agents that communicate with each other and work cooperatively to achieve common goals. Also, it is to medicate and coordinate communication between Control Areas and Security Coordinators for teal-time control of the BCHP system and the power pid. In this new circumstance, it is very important to integrate the power and energy delivery system and the information system(communication, networks, and intelligent equipment) that controls it. Therefore, development of smart control modules with open communication protocol and seamlessly interchange the data and information between control network and data network including extranet and intranet give a great meanings. We designed and developed the TCP/IP-CAN IED agent modules and ModBus./LonTalk/(TCP/IP) IED agent ones to configure the multi-agent system based smart energy network of commercial buildings and also intelligent algorithms for inverter fault diagnostics which ran be operated in control level or agent level network.

• 대한핵의학회지
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• 제37권1호
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• pp.43-52
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• 2003

Microarray technology allows the simultaneous analysis of gene expression patterns of thousands of genes, in a systematic fashion, under a similar set of experimental conditions, thus making the data highly comparable. In some cases arrays are used simply as a primary screen loading to downstream molecular characterization of individual gene candidates. In other cases, the goal of expression profiling is to begin to identify complex regulatory networks underlying developmental processes and disease states. Microarrays were originally used with ceil lines or other simple model systems. More recently, microarrays have been used in the analysis of more complex biological tissues including neural systems and the brain. The application of cDNA arrays in neuropsychiatry has lagged behind other fields for a number of reasons. These include a requirement for a large amount of input probe RNA In fluorescent-glass based array systems and the cellular complexity introduced by multicellular brain and neural tissues. An additional factor that impacts the general use of microarrays in neuropsychiatry is the lack of availability of sequenced clone sets from model systems. While human cDNA clones have been widely available, high qualify rat, mouse, and drosophilae, among others are just becoming widely available. A final factor in the application of cDNA microarrays in neuropsychiatry is cost of commercial arrays. As academic microarray facilitates become more commonplace custom made arrays will become more widely available at a lower cost allowing more widespread applications. in summary, microarray technology is rapidly having an impact on many areas of biomedical research. Radioisotope-nylon based microarrays offer alternatives that may in some cases be more sensitive, flexible, inexpensive, and universal as compared to other array formats, such as fluorescent-glass arrays. In some situations of limited RNA or exotic species, radioactive membrane microarrays may be the most practical experimental approach in studying psychiatric and neurodegenerative disorders, and other complex questions in the brain.

• Genes and Genomics
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• 제40권12호
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• pp.1363-1371
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• 2018

One of the main concerns in biology is extracting sophisticated features from DNA sequence for gene interaction determination, receiving a great deal of researchers' attention. The epigenetic modifications along with their patterns have been intensely recognized as dominant features affecting on gene expression. However, studying sequenced-based features highly correlated to this key element has remained limited. The main objective in this research was to propose a new feature highly correlated to epigenetic modifications capable of classification of genes. In this paper, classification of 34 genes in PPAR signaling pathway associated with muscle fat tissue in human was performed. Using different statistical outlier detection methods, we proposed that 5-mers highly correlated to epigenetic modifications can correctly categorize the genes involved in the same biological pathway or process. Thirty-four genes in PPAR signaling pathway were classified via applying a proposed feature, 5-mers strongly associated to 17 different epigenetic modifications. For this, diverse statistical outlier detection methods were applied to specify the group of thoroughly correlated genes. The results indicated that these 5-mers can appropriately identify correlated genes. In addition, our results corresponded to GeneMania interaction information, leading to support the suggested method. The appealing findings imply that not only epigenetic modifications but also their highly correlated 5-mers can be applied for reconstructing gene regulatory networks as supplementary data as well as other applications like physical interaction, genes prioritization, indicating some sort of data fusion in this analysis.

• The Korean Journal of Physiology and Pharmacology
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• 제26권5호
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• pp.357-365
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• 2022

Simultaneous myofibril and mitochondrial development is crucial for the cardiac differentiation of pluripotent stem cells (PSCs). Specifically, mitochondrial energy metabolism (MEM) development in cardiomyocytes is essential for the beating function. Although previous studies have reported that MEM is correlated with cardiac differentiation, the process and timing of MEM regulation for cardiac differentiation remain poorly understood. Here, we performed transcriptome analysis of cells at specific stages of cardiac differentiation from mouse embryonic stem cells (mESCs) and human induced PSCs (hiPSCs). We selected MEM genes strongly upregulated at cardiac lineage commitment and in a time-dependent manner during cardiac maturation and identified the protein-protein interaction networks. Notably, MEM proteins were found to interact closely with cardiac maturation-related proteins rather than with cardiac lineage commitment-related proteins. Furthermore, MEM proteins were found to primarily interact with cardiac muscle contractile proteins rather than with cardiac transcription factors. We identified several candidate MEM regulatory genes involved in cardiac lineage commitment (Cck, Bdnf, Fabp4, Cebpα, and Cdkn2a in mESC-derived cells, and CCK and NOS3 in hiPSC-derived cells) and cardiac maturation (Ppargc1α, Pgam2, Cox6a2, and Fabp3 in mESC-derived cells, and PGAM2 and SLC25A4 in hiPSC-derived cells). Therefore, our findings show the importance of MEM in cardiac maturation.

• 무역학회지
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• 제41권4호
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• pp.293-313
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• 2016

동아시아 지역은 FTA 확대로 인해 국경을 초월한 경제일체화가 진척되고 있고, 각국의 규제제도 개혁으로 기업활동의 자유는 높아지고 있으며, 국경을 초월한 공정간 분업이 진전되고 있는 등 무역네트워크의 고도화가 촉진되고 있다. 미국이 주도하는 다자간 FTA인 환태평양경제동반자협정(TPP)은 2016년 2월 4일 뉴질랜드 오클랜드에서 12개국이 협정문에 서명하였는데, 동아시아 무역네트워크의 변화가 예상된다. 본 연구에서는 TPP가 동아시아 무역네트워크에 미칠 영향에 관해 살펴보았다. 연구결과 TPP는 공급사슬의 글로벌화를 이끄는 규제 및 제도로써 공급사슬 구조를 변화시키고, 가치사슬에 긍정적인 효과를 야기한다. 이는 동아시아 무역네트워크에 상당한 영향을 미칠 것이며 참여기업의 경쟁력 강화로 이어질 것이다. 또한 TPP는 향후 아태자유무역지대(FTAAP)의 실현을 위한 토대가 될 것으로 보이는데, 무역의존도가 높은 한국은 이로 인해 변화할 동아시아 무역환경에 효과적으로 대처하기 위한 정책적 노력을 기울여야 할 것이다.

• Animal Bioscience
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• 제37권4_spc호
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• pp.775-785
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• 2024

Pork production is a significant agricultural enterprise in the United States and Canada. The United States is the third-largest global producer of pork and Canada ranks seventh in pork production. The North American Free Trade Agreement and its successor, the U.S.-Mexico-Canada Agreement, have facilitated trade and integration between the two countries. The majority of production systems are modern and intensive, characterized by large vertically integrated farms using advanced technologies. Both nations benefit from their status as major producers of feed grains, with the United States leading in corn and soybeans, while Canada excels in canola and barley production. The regulatory frameworks for food safety, animal welfare, and environmental stewardship differ slightly, with the FDA and USDA overseeing these aspects in the United States, and Health Canada and the Canada Food Inspection Agency in Canada. The United States and Canada also have well-established distribution networks for pork products, relying on both domestic and international markets. Export markets play a crucial role, with the United States being a major importer of Canadian pigs, and both countries exploring opportunities in Asia. Despite a rise in global demand, domestic pork consumption trends differ, with per capita consumption remaining stable in the USA and declining in Canada. Changing consumer preferences, including a demand for ethically raised and locally sourced pork, may influence production practices. Future trends in pig production include a focus on consumer concerns, sustainability, disease prevention, reduction of antimicrobial use, and advancements in technology. The industry is adapting to challenges such as disease outbreaks and changing regulations, with a strong emphasis on animal welfare. Labor and workforce considerations, along with advancements in technology and automation, are expected to shape the efficiency of pork production in the future.

• 한국빅데이터학회지
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• 제6권1호
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• pp.91-113
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• 2021

한국 자산 시장에서 부동산이 가지는 영향력에도 불구하고 시장 추이 예측은 쉽지 않으며, 그중 아파트는 주거 공간인 동시에 투자 속성을 내포하고 있어 더욱 예측이 쉽지 않다. 아파트 가격에 영향을 주는 요인은 다양하며 지역적 특성 또한 고려되어야 한다. 본 연구는 서울시 전체, 강남 3구, 노원, 도봉, 강북, 금천, 관악, 구로구의 아파트 매매가에 영향을 미치는 요인과 특성을 비교하고 이를 기반으로 가격 예측의 가능성을 파악하기 위해 수행되었다. 분석에는 신경망, CHAID, 선형회귀, 랜덤포레스트 등 머신러닝 알고리즘이 사용되었다. 서울시 전체 아파트 평균 매매가에 가장 중요한 영향을 미치는 요소는 정부 정책 요소였으며, 거래규제 완화, 금융규제 완화 등의 완화 정책이 영향력이 높게 도출되었다. 강남 3구의 경우 정책의 영향력이 낮은 것으로 파악되었으며 강남구의 경우 주택 공급량이 가장 중요한 요인이었다. 반면 6개의 중·하위구들은 정부 정책이 중요 변수로 작용하였으며 공통적으로 금융규제 정책이 영향을 끼치는 요인이었다.