• Korean Journal of Veterinary Research
• /
• v.56 no.2
• /
• pp.97-101
• /
• 2016

Emodin is an anthraquinone derivative from the roots of Rheum officinale Baill that possesses a variety of biological activities, including inhibition of $5{\alpha}$-reductase and prostaglandin D2. In this study, we investigated whether emodin promotes hair growth. After emodin was topically applied to the shaved dorsal skin of telogenic C57BL/6 N mice, the hair growth rate and morphological analysis were evaluated in dorsal skin for 15 days. After 13 days of treatment, minoxidil or emodin (0.01% or 0.1%)-treated groups showed remarkable regrowth of hairs relative to the vehicle control group. Scoring of the hair growth and rate of hair growth area for 15 days revealed that groups treated with minoxidil and 0.1% emodin were significantly higher than the vehicle control group. Histological examination revealed the emodin and minoxidil groups markedly recovered the number and morphology of hair follicles, including the subcutis depth, relative to the vehicle group. These results suggest that emodin has an excellent promoting effect in hair growth similar to that of minoxidil and might be useful for treatment of baldness or alopecia.

• Korean Journal of Plant Resources
• /
• v.33 no.6
• /
• pp.675-681
• /
• 2020

This study describes an efficient and stable droplet vitrification following cryopreservation of strawberry shoot tip (Fragaria × ananassa Duch.) accessions 'Massey' and 'MDUS3816'. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 17.5% glycerol and 17.5% sucrose for 40 min and exposed to dehydration solution (B1) containing 50% glycerol and 50% sucrose for 40 min at 25oC. Subsequently, the explants were transferred onto droplets containing 2.5 µL PVS3 on sterilized aluminum foils (4 cm× 0.5 cm) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with 0.3M sucrose for 30 h + 0.5M sucrose for 16 h at 25oC. The cryopreserved shoots tips exhibited 57.8 % recovery rate by culturing in NH₄NO₃-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0mg/L GA₃, and 0.5 mg/L BA for 5 weeks and in MS medium supplemented with 0.5 mg/L GA₃ for 8 weeks. Variation was not observed in both of ploidy analysis and morphological investigation on plantlets of two accessions cryopreserved under variable preculture conditions.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.13 no.4
• /
• pp.257-267
• /
• 1993

Optimum pasture management during the summer season is an important factor to maintain good regrowth and persistence of pasture. The field experiment was carried out to investigate the effects of cutting management on growth and reserve carbohydrates in stubble. and on dry matter yield of orchardgrass dominated pasture during the mid-summer season. Three different cutting times(July 15, 25 and August 5) as a main plot and three cutting heights(3, 6 and 10cm) as a sub plot were disigned with three replications. The experiment was done at pasture of Changsung Agricultural High School in 1988. The results obtained were summarized as follows: 1. Temperature of soil surface and underground in the cutting of July 15 and July 25 was not showed significantly different, because of low intensity of radiation with a run of wet weather, and that of August 5 cutting tends to be lowed in high cutting height during the mid-summer season. 2. Amount of soil moisture against cutting height showed that high cutting had a low content. 3. Growth rate against regrowth of plant height, lear length and leaf area showed to be fast in high cutting. 4. Rapid recovery period of carbohydrate content after cutting and large amount of carbohydrate accumulation were showed in the cutting of July 15 and 25 compared with August 5 cutting in the reserve carbohydrate content against cutting of time and height during the mid-summer season. 5. Dry matter yield of the 4th and 5th cutting showed to be remarkable in July 15 cutting compared to those of July 25 and August 5. From the above results, it is suggested that the 10cm cutting height during the mid-summer season is the most effective for good regrowth, reserve carbohydrates and dry matter yield of orchardgrass.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.11
• /
• pp.1619-1637
• /
• 2000

A review was undertaken to obtain information on the range of beak-trimming methods available or under development. Beak-trimming of commercial layer replacement pullets is a common yet critical management tool that can affect the performance for the life of the flock. The most obvious advantage of beak-trimming is a reduction in cannibalism although the extent of the reduction in cannibalism depends on the strain, season, and type of housing, flock health and other factors. Beak-trimming also improves feed conversion by reducing food wastage. A further advantage of beak-trimming is a reduction in the chronic stress associated with dominance interactions in the flock. Beak-trimming of birds at 7-10 days is favoured by Industry but research over last 10 years has shown that beak-trimming at day-old causes the least stress on birds and efforts are needed to encourage Industry to adopt the practice of beak-trimming birds at day-old. Proper beak-trimming can result in greatly improved layer performance but improper beak-trimming can ruin an other wise good flock of hens. Re-trimming is practiced in most flocks, although there are some flocks that only need one trimming. Given the continuing welfare scrutiny of using a hot blade to cut the beak, attempts have been made to develop more welfare friendly methods of beak-trimming. Despite the developments in design of hot blade beak-trimmers the process has remained largely unchanged. That is, a red-hot blade cuts and cauterises the beak. The variables in the process are blade temperature, cauterisation time, operator ability, severity of trimming, age of trimming, strain of bird and beak length. This method of beak-trimming is still overwhelmingly favoured in Industry and there appears to be no other alternative procedures that are more effective. Sharp secateurs have been used trim the upper beak of both layers and turkeys. Bleeding from the upper mandible ceases shortly after the operation, and despite the regrowth of the beak a reduction of cannibalism has been reported. Very few differences have been noted between behaviour and production of the hot blade and cold blade cut chickens. This method has not been used on a large scale in Industry. There are anecdotal reports of cannibalism outbreaks in birds with regrown beaks. A robotic beak-trimming machine was developed in France, which permitted simultaneous, automated beak-trimming and vaccination of day-old chicks of up to 4,500 chickens per hour. Use of the machine was not successful because if the chicks were not loaded correctly they could drop off the line, receive excessive beak-trimming or very light trimming. Robotic beak-trimming was not effective if there was a variation in the weight or size of chickens. Capsaicin can cause degeneration of sensory nerves in mammals and decreases the rate of beak regrowth by its action on the sensory nerves. Capsaicin is a cheap, non-toxic substance that can be readily applied at the time of less severe beak-trimming. It suffers the disadvantage of causing an extreme burning sensation in operators who come in contact with the substance during its application to the bird. Methods of applying the substance to minimise the risk to operators of coming in contact with capsaicin need to be explored. A method was reported which cuts the beaks with a laser beam in day-old chickens. No details were provided on the type of laser used, or the severity of beak-trimming, but by 16 weeks the beaks of laser trimmed birds resembled the untrimmed beaks, but without the bill tip. Feather pecking and cannibalism during the laying period were highest among the laser trimmed hens. Currently laser machines are available that are transportable and research to investigate the effectiveness of beak-trimming using ablasive and coagulative lasers used in human medicine should be explored. Liquid nitrogen was used to declaw emu toes but was not effective. There was regrowth of the claws and the time and cost involved in the procedure limit the potential of using this process to beak-trim birds.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.59 no.4
• /
• pp.498-504
• /
• 2014

Potato virus Y (PVY) and potato leafroll virus (PLRV) are among the most damaging potato viruses and prevalent in most potato growing areas. In this study, cryopreservation was used to eradicate PVY and PLRV using two cryogenic methods. Potato shoot tips proliferated in vitro were cryopreserved through droplet-vitrification and encapsulation-vitrification using plant vitrification solution 2 (PVS2; 30% glycerol + 15% dimethyl sulfoxide + 15.0% ethylene glycol + 13.7% sucrose) and modified PVS2. Both cryogenic procedures produced similar rates of survival and regrowth, which were lower than those from shoot tip culture alone. The health status of plantlets regenerated from shoot tip culture alone and cryopreservation was checked by reverse transcription-polymerase chain reaction. The frequency of virus-free plants regenerated directly from highly proliferating shoot tips reached 42.3% and 48.6% for PVY and PLRV, respectively. In comparison, the frequency of PVY and PLRV eradication after cryopreservation was 91.3~99.7% following shoot-tip culture. The highest cryopreserved shoot tip regeneration rate was observed when shoot tips were 1.0~1.5 mm in length, but virus eradication rates were very similar (96.4~99.7%), regardless of shoot tip size. This efficient cryotherapy protocol developed to eliminate viruses can also be used to prepare potato material for safe long-term preservation and the production of virus-free plants.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.8 no.2
• /
• pp.104-109
• /
• 1988

A field experiment was conducted in order to investigate the seasonal changes of leaf grwoth and related characteristics in three cultivars of orchardgrass; Potomac, Kay and Sumas. The results were summarized as follows: 1. Leaf elongation was increased in a nearly linear phase during first and third cutting stages. It was increased slowly in early 10 days to 15 days after cutting and increased rapidly there-after during the rest cutting stages. In cultivars, Potomac was showed higher leaf elongation than other cultivars during all cutting stages. There was no difference of leaf width within cutting stages, but the leaf width of fall regrwoth was narrow. Sumas had relatively short and wide leaves. 2. Leaf dry weight and leaf area in first cutting stage were larger than others. Leaf area was increased rapidly form 15 days after cutting and leaf weight was increased rapidly from 20 days over all cutting stages. The increase in leaf area and dry weight were slow down after 30 days. 3. Number of epidermal cells was increased rapidly after cutting and the rate of increase was slow down after 30 days. In a cross section of leaf tissue, the part of mesophyll was occupied with about 60% of total area and larger area than other tissue, the part of mesophyll was occupied with about 60% of total area and larger area than other tissues. Leaf tissue had a large vacancy at early growth period after harvest and was filled gradually with mesophyll. This result was related to the increase of leaf dry matter.

• Journal of Korean Neurosurgical Society
• /
• v.30 no.12
• /
• pp.1381-1387
• /
• 2001

Objective : Endodermal sinus tumor or yolk sac tumor is an uncommon malignant germ-cell neoplasm. This tumor was originally described as a germ cell tumor of the ovary or the testis. Intracranial endodermal sinus tumor is extremely rare and usually develop in the pineal or suprasellar regions. The authors evaluated the effect of adjuvant therapy(chemotherapy combined with radiotherapy) and radical removal of intracranial endodermal sinus tumors. Material and Methods : Between 1996 and 2001, four patients of intracranial endodermal sinus tumor were diagnosed with tumor marker(AFP) and biopsy. Three patients were treated with surgical removal and chemotherapy with cisplatin($20mg/m^2$), etoposide($100mg/m^2$) and bleomycin($15mg/m^2$) as well as external beam radiation therapy. We compared the management problems for these tumors. Result : In all three patients the tumor size and the level of tumor marker decresed during initial adjuvant therapy. However, Tumors showed regrowth with elevated AFP of serum and CSF possibly related to delayed chemotherapeutic treatment or inadequate administration of chemotherapeutic drugs due to severe bone marrow suppression. An additional chemotherapy and external radiation therapy were given, but tumors could not be controlled with leptomeningeal seeding. Conclusion : Radiotherapy is considered to be less effective. The combination chemotherapy with PVB(cisplatin, vinblastine, bleomycine) or PE(cisplatin, etoposide) is considered to be value in prolongation of the survival rate. But the role of chemotherapy in this tumor has not yet been clarified due to bone marrow suppression and drug resistance. Further study with large series of this tumor is necessary to establish the optimal management.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.26 no.3
• /
• pp.257-262
• /
• 1981

Field experiment with 0, 100, 200, 400, and 800 kg-N/ha-year application levels was carried out to study the nitrogen response in the early stage of orchardgrass pasture establishment at College Fann, SNU, in 1979 and 1980. Both the highest dry matter yield and maximum percent of N recovery were obtained at the same level of 200 kg-N/ha in the year of seeding. but those of the next year were obtained at 400 kg-N/ha level. Leaf area indices (LAI) and net assimilation rates (NAR) during each regrowth periods as well as total nitrogen contents of forage at each cutting time increased with applied N in both years. The maximum crop growth rate (CGR) over two years was estimated to be obtained when LAI reached to about 5. The accumulation of NO_3 -N in forage started from 400kgㆍN/ha application in 1980. and exceeded the safe level for ruminants at the level of 800 kg-N/ha.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.251-251
• /
• 2017

This study was performed to select the soil covering materials for winter safety of Cynanchum wilfordii Hemsley and to investigate the effect on the growth after overwinter. Soil covering materials such as vinyl, rice straw, lagging cover, vinyl+lagging cover, and non-covering were investigated how effect on overwintering rate, growth and yield of Cynanchum wilfordii Hemsley. In changes of soil temperature according to the soil covering materials, non-covering treatment was recorded the highest to $26.7^{\circ}C$, and lagging cover and rice straw showed little change of soil temperature with 9.2 and $9.3^{\circ}C$, respectively. Regrowth rates of early growth after overwinter were lowered in the order as rice straw > vinly+lagging cover > lagging cover > vinyl > non-covering. From the above-ground growth by soil covering materials, vine length showed similar growth in all treatments except vinyl covering and stem diameter exhibited the thicker pattern in vinyl and lagging cover, and there was no significant difference among treatments in number of branch. Flowering period is recorded July 30 in all treatments and ripening period is October 16. Yield of seed production is showed the best in vinyl+lagging cover with $25g/m^2$. Root weight and root length for subterranean growth showed no significant differences among all treatments. Yield is produced the highest in vinyl+lagging cover with 433kg/10a and the next to lagging cover with 431kg/10a. In comparison with incomes, lagging cover earned to 9,882 thousand won/10a, showed the effect on income enlargement to 21%, compare to that of the non-covering.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.32 no.6
• /
• pp.530-543
• /
• 2006

Adeonoid cystic carcinoma (ACC) is one of the most common malignant tumors of salivary glands. It is characterized by a relentless regrowth especially around nerve tissues and a high rate of hematogenous distant metastasis. Clinically most deaths from salivary ACC are caused by delayed lung metastases that are resistant to conventional chemotherapy. So, knowledge of cellular and molecular properties that influence the dissemination of metastatic tumor cells, is important for new treatment strategies of metastatic lesions. We determined expressions of angiogenic signaling molecules microvessel density (MVD) using surgical specimens of human salivary ACC. Protein expressions of vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR)-2, activated VEGFR-2, and human CD31 were assessed in 20 cases of salivary ACC by immunohistochemical staining. Most of the tumors, especially ACC with a tubulocribriform pattern, were positive for antibodies of VEGF, VEGFR-2, and activated VEGFR-2. The overall percentages of the 20 specimens expressing VEGF, VEGFR-2, activated VEGFR-2 were 90, 95, and 95%, respectively. Immunoreactivities of the biomarkers in salivary ACC were higher than those in normal salivary gland. Furthermore, immune-related cells as well as tumor cells expressed VEGF/VEGFR-2. Microvessel density of salivary ACC was higher than that of normal salivary gland (P<0.05). Taken together, angiogenic signaling molecules are actively expressed in salivary ACC. And we suggest that these molecules may have critical role in the hematogenous spread of salivay ACC, which has a propensity for delayed lung metastasis. Therefore, these biomarkers can be molecular targets for therapy of metastasis of salivary ACC.