• 식품과학과 산업
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• 제50권1호
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• pp.11-15
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• 2017

Epigenomics is a study that analyzes and quantifies various epigenetic alterations that affect gene expressions in cells from the viewpoint of collective characteristics on biological molecular pools. DNA methylation and histone modification in cells can induce the epigenetic alterations. Especially, epigenetic alterations influenced by external factors as ingested foods and other environmental factors have been examined in the whole genome regions, which provide accumulated data of altered regions or patterns of global genome, Statistical analyses of these regions or patterns enables us to correlate epigenomic changes with human diseases in the whole genome region. Finding meaningful regulators is a major concern of epigenomic research in recent years, and these results will give the food industry an important clue to future food

• 한국자기공명학회논문지
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• 제23권4호
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• pp.93-97
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• 2019

Black ginger (Kaempferia parviflora) is a short-lived ginger plant with dark purple colored root and is known to be effective in treating diabetes and obesity. To find out the difference in the characteristics of the black ginger according to the variety of production, 1D proton NMR experiments were performed on 4 types of black gingers from different regions. The NMR spectra of all black ginger showed the characteristic peaks of the polymethoxy flavone compounds, and the chemical shifts and intensity of peaks showed slight differences depending of the type of black ginger implying the difference in molecular environment. These initial NMR experiments can be applied to the identification of the diversity of black ginger in physiological function according to the climate of regions using SNIF-NMR (Site-specific Natural Isotope Fractionation studied by NMR).

• Clinical and Experimental Reproductive Medicine
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• 제28권2호
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• pp.141-145
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• 2001

Objective: We inversigated Small Heterodimer Partner (SHP) gene mutation in Korean Polycystic Ovarian Syndrome (PCOS) patients. SHP protein regulates the activity of nuclear receptors which regulate the cellular development and differentiation. Recently, the mutation of SHP gene was found in the obesity and diabetes patients in Japanese group, and suggested that its mutation may involved in pathogenic mechanism of PCOS. Methods: This study was performed in 20 PCOS patients and 20 normal women. The DNAs were extracted from the peripheral bloods, and amplified at each exon (1 and 2) of SHP gene by PCR method. Subsequently, each PCR product was digested with the restriction enzyme indicated below for studying restriction fragment length polymorphism (RFLP). After enzyme digestion, the results of RFLP were compared PCOS patients with control women to find any sequence variation. Results: We examined 9 regions of exon 1 with Msp I, Pvu II, Dde I and 3 regions of exon 2 with Pst I, Dde I. There is no heterozygous or homozygous mutation in patients and control women at these restriction sites. Conclusion: The genetic analysis at our restriction sites in the SHP gene did not show any genetic variation in Korean PCOS patients. Our PCR-RFLP analysis was not covered the entire SHP gene (68 bp/1,006 bp), we need to further analysis of the entire SHP gene.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2005년도 춘계종합학술대회
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• pp.828-831
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• 2005

본 논문에서는 얼굴을 검출하기 위해서 컬러영상에서 눈동자 템플릿을 사용한 얼굴을 검출하는 방법에 관해 제안하였다. 전체 시스템의 구성은 크게 피부색 모델에 의한 피부 영역을 검출하고 검출된 피부 영역에 타원을 적용하여 얼굴영역을 찾은 뒤 템플릿을 사용하여 눈동자를 추출하여 정규화 된 얼굴을 검출하는 단계로 이루어 졌다. 특히 타원을 적용할 때와 눈동자 템플릿을 적용할 때는 모멘트를 사용하였으며, 눈동자를 추출할 때 벡터를 기반으로 하기 때문에 사이즈에 제한 없이 자유로운 변형이 가능할 뿐만 아니라 눈동자 템플릿을 사용하면 눈동자의 위치와 크기를 동시에 얻어 낼 수 있었다. 마지막으로 이렇게 검출된 얼굴을 기울어진 각도만큼 회전시켜 정규화 된 얼굴을 검출 할 수 있었다.

• 한국지능시스템학회논문지
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• 제13권4호
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• pp.461-468
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• 2003

In a sequence of images obtained by surveillance cameras, facial regions appear very small and their colors change abruptly by lighting condition. This paper proposes a new face detection scheme, robust on complex background, small size, and lighting conditions. The proposed method is consisted of three processes. In the first step, the candidates for the face regions are selected using face color distribution and motion information. In the second stage, the non-face regions are removed using face color ratio, boundary ratio, and average of column-wise intensity variation in the candidates. The face regions containing eyes and mouth are segmented and classified, and then they are scored using their topological relations in the last step. To speed up and improve a performance the above process, a block based image segmentation technique is used. The experiments have shown that the proposed algorithm detects faced regions with more than 91% of accuracy and less than 4.3% of false alarm rate.

• Clinical and Experimental Reproductive Medicine
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• 제40권1호
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• pp.1-6
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• 2013

Objective: Oocyte-specific homeobox 4 (Obox4) is preferentially expressed in oocytes and plays an important role in the completion of meiosis of oocytes. However, the Obox4 expression pattern has not been reported yet. In this study, we investigated the subcellular localization of Obox4 using a green fluorescent protein (GFP) fusion expression system. Methods: Three regions of Obox4 were divided and fused to the GFP expression vector. The partly deleted homeodomain (HD) regions of Obox4 were also fused to the GFP expression vector. The recombinant vectors were transfected into HEK-293T cells plated onto coated glass coverslips. The transfected cells were stained with 4',6-diamidino-2-phenylindol and photographed using a fluorescence microscope. Results: Mutants containing the HD region as well as full-length Obox4 were clearly localized to the nucleus. In contrast, the other mutants of either the N-terminal or C-terminal region without HD had impaired nuclear localization. We also found that the N-terminal and C-terminal of the Obox HD contributed to nuclear localization and the entire HD was necessary for nuclear localization of Obox4. Conclusion: Based on the results of the present study, we demonstrated that the intact HD region of Obox4 is responsible for the nuclear localization of Obox4 protein in cells.

• Journal of Yeungnam Medical Science
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• 제40권4호
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• pp.381-387
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• 2023

Background: Frailty is defined as a condition of being weak and delicate, and it represents a state of high vulnerability to adverse health outcomes. Recent studies have suggested that the cingulate gyrus is associated with frailty in the elderly population. However, few imaging studies have explored the relationship between frailty and the cingulate gyrus in patients with end-stage renal disease (ESRD) undergoing hemodialysis. Methods: Eighteen right-handed patients with ESRD undergoing hemodialysis were enrolled in the study. We used the FreeSurfer software package to estimate the cortical thickness of the regions of interest, including the rostral anterior, caudal anterior, isthmus, and posterior cingulate gyri. The Beck Depression Inventory, Beck Anxiety Inventory, and laboratory tests were also conducted. Results: The cortical thickness of the right rostral anterior cingulate gyrus (ACG) was significantly correlated with the Fried frailty index, age, and creatinine level. Multiple regression analysis indicated that the cortical thickness of the right rostral ACG was associated with frailty after controlling for age and creatinine level. Conclusion: Our results indicate that the cortical thickness of the rostral ACG may be associated with frailty in patients with ESRD on hemodialysis and that the rostral ACG may play a role in the frailty mechanism of this population.

• Clinical and Experimental Reproductive Medicine
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• 제44권3호
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• pp.152-158
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• 2017

Objective: To identify the associations between polymorphisms of the 3'-untranslated region (UTR) of methylenetetrahydrofolate reductase (MTHFR) gene, which codes for an important regulatory enzyme primarily involved in folate metabolism, and idiopathic recurrent pregnancy loss (RPL) in Korean women. Methods: The study population comprised 369 RPL patients and 228 controls. MTHFR 2572C > A, 4869C > G, 5488C > T, and 6685T > C 3'-UTR polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis or by TaqMan allelic discrimination assays. Natural killer cell proportions were determined by flow cytometry. Results: The MTHFR 2572-5488-6685 (A-C-T) haplotype had an adjusted odds ratio of 0.420 (95% confidence interval, 0.178-0.994; p= 0.048) for RPL. Analysis of variance revealed that MTHFR 4869C > G was associated with altered $CD56^+$ natural killer cell percentages (CC, $17.91%{\pm}8.04%$; CG, $12.67%{\pm}4.64%$; p= 0.024) and folate levels (CC, $12.01{\pm}7.18mg/mL$; CG, $22.15{\pm}26.25mg/mL$; p= 0.006). Conclusion: Variants in the 3'-UTR of MTHFR are potential biomarkers for RPL. However, these results should be validated in additional studies of ethnically diverse groups of patients.

• 대한산업공학회지
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• 제19권3호
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• pp.139-154
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• 1993

Cutter Interference(or part surface gouging) is one of the most critical problems in NC machining of sculptured surfaces. Presented in this paper is and algorithmic procedure that converts CC data obtained from a compound surface(several surfaces without topological relationship) into interference-free CL data. The interference handling procedure consists of following steps: (1) Z-map model is constructed from input surfaces. (2) Interference sources are detected using local properties of the sources. (3) Interference regions are completely identified based on global tests for neighboring CC points of the interference sources (4) Cutter paths are reconstructed after removing the CC data in interference regions, while avoiding any new interferences.

• 대한임베디드공학회논문지
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• 제5권2호
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• pp.46-56
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• 2010

This study proposes a monitoring system by cooperating the image information and the sensor information in a sensor network system. The monitoring system proposed in this study is divided into internal spaces, such as offices and laboratories, and external spaces including other various spaces. In the internal spaces, motions in objects are detected through cameras while some peripherals like lights are controlled by analyzing some temperature, humidity, and illuminance data detected by sensor nodes. In the external spaces, it is to watch certain intruders to the internal spaces through the interested region for exceptional time by installing cameras, motion detectors, and body detectors in such interested regions. In the results of the test that was applied to a practically limited environment by implementing some interfaces for the proposed system, it was considered that it is possible to watch surroundings effectively using the image information obtained from cameras and sensor information acquisited from sensor nodes.