• 제목/요약/키워드: regenerative process

검색결과 144건 처리시간 0.031초

추력 30톤급 액체산소/케로신 로켓엔진 연소장치 개발(I)-연소기 (Development of 30-Tonf LOx/Kerosene Rocket Engine Combustion Devices(I) - Combustion Chamber)

  • 최환석;한영민;김영목;조광래
    • 한국항공우주학회지
    • /
    • 제37권10호
    • /
    • pp.1027-1037
    • /
    • 2009
  • 우주발사체용 30톤급 액체로켓엔진 재생냉각 연소기 개발과정에 관하여 기술하였다. 이중추진제 동축 와류형 분사기 개발에서부터 시작하여 축소형 연소기를 통해 요소기술을 검증하고 개발된 기술을 실물형 연소기에 적용하였다. 총 5기의 실물형 연소기 개발시제를 사용하여 점화성능, 연소안정성, 연소성능, 냉각성능, 내구성 등의 검증을 수행하였다. 이 과정에서 총 46회의 실물형 연소기 연소시험을 실시하였고 이 중 23회는 기폭장치를 이용한 연소안정성 평가시험을 병행하였다. 시험 결과 30톤급 재생냉각 연소기는 연소 성능 및 연소안정성 요구사항을 모두 만족시켜 단품 개발이 성공적으로 완료된 것으로 평가하였다.

소성 Dolomite의 수화 및 탈수반응을 이용한 축열에 관한 연구 (Studies on the Heat Storage Using the Hydration/Dehydration of the Calcined Dolomite)

  • 이수각;이영세;김종식
    • 공업화학
    • /
    • 제3권3호
    • /
    • pp.507-515
    • /
    • 1992
  • 본 연구에서는 소성 dolomite와 수증기계의 열화학 반응을 축열식열교환기에 응용하기 위해 충전층 형태의 실험장치에서 축열 및 방열특성에 관한 연구를 수행하였다. 실험 data는 수화반응온도 $150-400^{\circ}C$, 탈수반응온도 $700-800^{\circ}C$ 및 수증기 유량 294, 430, 567 g/hr의 실험조건에서 얻은 결과이다. 본 연구의 실험범위내에서는 소성 dolomite중 MgO가 수화반응 과정중 수화되지 않음을 알았다. 따라서 소성된 dolomite중 MgO는 불활성 물질로 취급할 수 있다. 또한 율속단계는 충전층의 입구와 벽면으로부터, 출구와 중심쪽으로 반응이 진행되므로 반응율속보다는 열전달율속으로 진행된다고 생각된다.

  • PDF

Essential Guidelines for Manufacturing and Application of Organoids

  • Sun-Ju Ahn;Sungin Lee;Dayeon Kwon;Sejeong Oh;Chihye Park;Sooyeon Jeon;Jin Hee Lee;Tae Sung Kim;Il Ung Oh
    • International Journal of Stem Cells
    • /
    • 제17권2호
    • /
    • pp.102-112
    • /
    • 2024
  • An organoid is a self-organized three-dimensional structure derived from stem cells that mimics the structure, cell composition, and functional characteristics of specific organs and tissues and is used for evaluating the safety and effectiveness of drugs and the toxicity of industrial chemicals. Organoid technology is a new methodology that could replace testing on animals testing and accelerate development of precision and regenerative medicine. However, large variations in production can occur between laboratories with low reproducibility of the production process and no internationally agreed standards for quality evaluation factors at endpoints. To overcome these barriers that hinder the regulatory acceptance and commercialization of organoids, Korea established the Organoid Standards Initiative in September 2023 with various stakeholders, including industry, academia, regulatory agencies, and standard development experts, through public and private partnerships. This developed general guidelines for organoid manufacturing and quality evaluation and for quality evaluation guidelines for organoid-specific manufacturing for the liver, intestines, and heart through extensive evidence analysis and consensus among experts. This report is based on the common standard guideline v1.0, which is a general organoid manufacturing and quality evaluation to promote the practical use of organoids. This guideline does not focus on specific organoids or specific contexts of use but provides guidance to organoid makers and users on materials, procedures, and essential quality assessment methods at end points that are essential for organoid production applicable at the current technology level.

질 클레망의 정원관(庭園觀)과 '움직이는 정원'에 대한 연구 - William Robinson의 Wild Garden과의 개념비교를 중심으로 - (A Study on Gilles Clement's Garden View and the 'Garden in motion' - Centering on Conceptual Comparison with William Robinson's Wild Garden -)

  • 권진욱
    • 한국전통조경학회지
    • /
    • 제36권2호
    • /
    • pp.46-53
    • /
    • 2018
  • 본 연구는 동시대에서 요구되는 재생적 환경특성을 고려하고, 지속가능한 정원디자인을 실현하기 위한 개념적 바탕과 적용의 기초를 마련하는 것을 배경으로 한다. 그러므로 본 연구는 로빈슨의 자연주의적 가치관으로부터 영향을 받은 클레망의 정원이론에 대한 개념을 이해하며, 클레망의 정원에 구현된 자연주의적 차별특성을 로빈슨의 와일드가든과 비교하였으며 결과는 다음과 같다. 첫째, 클레망에게 있어서 정원디자인을 한다는 것은 식물의 움직임에 의해 형성되는 공간에 대한 과정의 미학으로 대상지에 정착하여 가는 생태적 프로세스를 포함한다. 그러므로 '움직이는 정원'을 실현한다는 것은 자연에 대한 신뢰를 바탕으로 자연에 순응하고, 식물들의 종자가 계획되지 않고 자연스럽게 적절한 생육환경을 찾아가도록 허용해야 하는 가능성을 디자인하는 것이다. 둘째, 야생(Wild)에 대한 가치관은 미시적 관점과 거시적 관점으로 구분되며 각각의 표현특성을 지니는데, 로빈슨의 경우 소규모의 정원으로서 기존 공간에 대한 연화(軟化) 기능을 담당하며 종속적인 특징을 가진다. 반면 클레망의 정원은 공간의 주체로서 거시적 안목에서의 전략이며, 생태적 역할의 중심이 되는 인프라스트럭쳐로서 구체화된 하향식 접근 특성을 가진다. 셋째, 로빈슨과 클레망의 정원에 대한 공간운용 관점은 자연의 자율성과 정원의 야생에 대한 불가분의 관계를 인정하며 자연에 대한 보존이 성립되어질 때 우리가 원하는 삶과 공존할 수 있다는 가치를 전달하며, 그들의 정원은 환경결정론의 관점을 바탕으로 식물이 매체가 된 환경가능론적 논지와 생태학적 입장의 중재로 유추된다.

초분, 한국 이중장제의 분석심리학적 고찰 : 부패와 뼈로의 환원을 중심으로 (CHOBUN, Understanding the Double Burial Custom in Korea from a Jungian Perspective : Focusing on Putrefaction and Reduction to Bones)

  • 조자현
    • 심성연구
    • /
    • 제31권2호
    • /
    • pp.113-150
    • /
    • 2016
  • 초분은 살이 다 썩어 없어질 때까지 관에 넣은 시신을 짚으로 싸두는 초가 형태의 임시 무덤을 말한다. 모든 살이 다 없어지고 나면, 초분은 해체되고, 뼈만 추려 다시 묻는다. 이러한 방식의 초분은 따라서 처음 시신의 살을 처리하기 위한 임시 매장과 이후 남은 부분(뼈나 재)을 영구히 매장 하는 이중장제에 속한다. 초분은 사람이 잘 가지 않고, 집에서도 떨어져 있지만, 유족이 가서 돌볼 수 있는 곳을 택해 만들어지는데, 심리학적으로 이것은 지속적인 관심을 쏟을 수 있으면서도 고요히 격리될 수 있는 곳, 다시 말해 심리적인 재생이 일어나는 장소이다. 초분을 두는 기간, 일차장의 기간을 정하는데 가장 중요한 점은 뼈만 남길 때까지 기다려야 하는 기간이 얼마나 되는지, 그리하여 초분을 해체하였을 때 완전한 뼈 상태를 확인할 수 있는지와 관련된 것이다. 여기서 초분이 부패와 뼈로의 환원을 목표로 하고 있음을 알 수 있다. 부패와 새로운 생명의 탄생은 연금술 문헌에서 보듯 동시에 일어난다. 부패의 최종 목적은 육신을 영적 상태로 만들고, 그리하여 죽은 자를 다른 삶으로 들어갈 수 있게 만들어 주는 것이다. 그러기 위해서는 아주 불안정한 상태에서, 부패를 견뎌내고, 썩는 냄새가 모두 사라지는 것을 기다려야 한다. 썩히는 것은 그리하여 육화된 세계, 몸을 용해시켜 없애고, 그 핵심, 열매만을 남기는 것을 목표로 한다. 부패의 과정은 자기 스스로의 오염되고 끔찍한 측면을 받아들이는 태도이며, 무력하고 수동적인 자세를 취함으로써, 새로운 생명이 오게하는 것이다. 원형적인 세계, 무의식은 우리가 접근하려 할 때, 종종 위협적이고 공격적인, 더러운 무엇으로 경험된다. 개성화 과정에서, 우리가 우리 정신의 이 끔찍하고 오염된 부분들을 볼 수 있는 용기를 낼 때만이, 무의식은 우리에게 새로운 영적 각성과 새로운 삶의 감각이라는 축복을 주는데, 이것이 부패가 의미하는 것이다. 뼈와 골격은 생명의 부술 수 없는, 소멸되지 않는 본질적인 요소를 상징한다. 뼈는 재생을 위한 최소 단위이자, 재생의 바탕이 되며, 여기서 새로운 생명이 자라나게 된다. 뼈의 상태로 환원은 생명의 바로 그 원천으로 돌아감을, 자궁으로 다시 들어가 심리적으로 자신의 자아중심성을 버리고, 자기가 개성화의 전 과정을 이끌도록 허용하는 것이다. 앞으로의 발달을 위해, 골격 상태로의 환원의 어려운 과정을 겪는 것은, 자아의 죽음의 선언이며, 그 목적은 자신을 썩기 쉽고 덧없는 살과 피, 육신에서 해방시키고, 전체로서의 영적인 갱신, 생명의 불멸의 요소를 얻기 위함이다. 초분은 또한 식물의 순환에서 해마다 보이는 부패와 생명의 부활을 보여준다. 초분에서는 이러한 식물의 순환의 상징이 다른 일반적인 한국 전통 상장례에서보다 명확하고 강하게 드러나는데, 죽음에도 살아남는 생명의 부분을 식물의 상징을 통해 경험하게 한다. 초분과 관련된 식물은 죽음 후의 생명의 지속성, 심리적으로 말해, 자기의 존재를 말한다. 초분에서 드러나는 풍부한 식물의 이미지들은, 모든 것이 사라진 죽음의 상태 너머에 존재하는 생명의 존재와 관련되어 있으며, 심리학적으로 우리의 삶에 정신에 끝없는 에너지를 공급하는 영원히 존재하며 의식을 다시 태어나게 하고 새롭게 하는 자기의 존재를 이야기한다.

염료감응형 태양전지용 질산 전처리된 $TiO_2$ 광전극의 전기화학적 특성 (Electrochemical Properties of HNO3 Pre-treated $TiO_2$ Photoelectrode for Dye-SEnsitized Solar Cells)

  • 박경희;김은미;구할본
    • 한국전기전자재료학회:학술대회논문집
    • /
    • 한국전기전자재료학회 2009년도 하계학술대회 논문집
    • /
    • pp.441-441
    • /
    • 2009
  • Dye-sensitized solar cells (DSSCs) have been widely investigated as a next-generation solar cell because of their simple fabrication process and low coats. The cells use a porous nanocrystalline TiO2 matrix coated with a sensitizer dye that acts as the light-harvesting element. The photo-exited dye injects electrons into the $TiO_2$ particles, and the oxide dye reacts with I- in the electrolyte in regenerative cycle that is completed by the reduction of $I_3^-$ at a platinum-coated counter electrode. Since $TiO_2$ porous film plays a key role in the enhancement of photoelectric conversion efficiency of DSSC, many scientists focus their researches on it. Especially, a high light-to-electricity conversion efficiency results from particle size and crystallographic phase, film porosity, surface structure, charge and surface area to volume ratio of porous $TiO_2$ electrodes, on which the dye can be sufficiently adsorbed. Effective treatment of the photoanode is important to improve DSSC performance. In this paper, to obtain properties of surface and dispersion as nitric acid treated $TiO_2$ photoelectrode was investigate. The photovoltaic characteristics of DSSCs based the electrode fabricated by nitric acid pre-treatment $TiO_2$ materials gave better performances on both of short circuit current density and open circuit voltage. We compare dispersion of $TiO_2$ nanoparticles before and after nitric acid treatment and measured Ti oxidized state from XPS. Low charge transfer resistance was obtained in nitric acid treated sample than that of untreated sample. The dye-sensitized solar cell based on the nitric acid treatment had open-circuit voltage of 0.71 V, a short-circuit current of 15.2 mAcm-2 and an energy conversion efficiency of 6.6 % under light intensity of $100\;mWcm^{-2}$. About 14 % increases in efficiency obtained when the $TiO_2$ electrode was treated by nitric acid.

  • PDF

The healing effect of platelet-rich plasma on xenograft in peri-implant bone defects in rabbits

  • Peng, Wang;Kim, Il-kyu;Cho, Hyun-young;Seo, Ji-Hoon;Lee, Dong-Hwan;Jang, Jun-Min;Park, Seung-Hoon
    • Maxillofacial Plastic and Reconstructive Surgery
    • /
    • 제38권
    • /
    • pp.16.1-16.9
    • /
    • 2016
  • Background: The association of biomaterial combined with repair factor-like platelet-rich plasma (PRP) has prospective values. Bovine-derived xenograft has been identified as an osteoconductive and biocompatible grafting material that provides osseointegration ability. PRP has become a valuable adjunctive agent to promote healing in a lot of dental and oral surgery procedures. However, there are controversies with respect to the regenerative capacity of PRP and the real benefits of its use in bone grafts. The purpose of this study was to assess the influence of PRP combined with xenograft for the repair of peri-implant bone defects. Methods: Twelve rabbits were used in this study, and the experimental surgery with implant installation was performed simultaneously. Autologous PRP was prepared before the surgical procedure. An intrabony defect (7.0 mm in diameter and 3.0 mm deep) was created in the tibia of each rabbit; then, 24 titanium dental implants (3.0 mm in diameter and 8.5 mm long) were inserted into these osteotomy sites. Thus, a standardized gap (4.0 mm) was established between the surrounding bony walls and the implant surface. The gaps were treated with either xenograft alone (control group) or xenograft combined with PRP (experimental group). After healing for 1, 2, 3, 4, 5, and 6 weeks, the rabbits were sacrificed with an overdose of KCl solution. Two rabbits were killed at each time, and the samples including dental implants and surrounding bone were collected and processed for histological analysis. Results: More newly formed bone and a better bone healing process were observed in control group. The histomorphometric analysis revealed that the mean percentage of bone-to-implant contact in the control group was significantly higher than that of the experimental group (25.23 vs. 8.16 %; P < 0.05, independent-simple t test, analysis of variance [ANOVA]). Conclusions: The results indicate that in the addition of PRP to bovine-derived xenograft in the repair of bone defects around the implant, PRP may delay peri-implant bone healing.

Pleiotrophin이 골수 줄기 세포의 부착 및 골형성에 미치는 효과에 대한 연구 (PLEIOTROPHIN EFFECTS ON BINDING AND SUBSEQUENT OSTEOGENESIS OF HUMAN MESENCHYMAL STEM CELLS)

  • 윤정호;윤정주;장현석;임재석;이의석;김대성;권종진
    • Maxillofacial Plastic and Reconstructive Surgery
    • /
    • 제28권2호
    • /
    • pp.111-117
    • /
    • 2006
  • An area of current research is investigating the app1ication of human mesenchymal stem cells or hMSCs as a cell-based regenerative therapy. In order to achieve effective bone regeneration, appropriate matrices functioning as cell-carriers must be identified and optimized in terms of function, efficacy and biocompatibility. Two methods of approaching optimization of matrices are to facilitate adhesion of the donor hMSCs and furthermore to facilitate recruitment of host progenitor cells to osteoblastic differentiation. Pleiotrophin is an extracellular matrix protein that was first identified in developing rat brains and believed to be associated with developing neuronal pathways. A recent publication by Imai and colleagues demonstrated that transgenic mice with upregulated pleiotrophin expression developed a greater volume of cortical as well as cancellous bone. The proposed mechanism of action of pleiotrophin is demonstrated here. Through either environmental stresses and/or intracellular regulation, there is an increase in pleiotrophin production. The pleiotrophin is released extracellularly into areas requiring bone deposition. A receptor-mediated process recruits host osteoprogenitor cells into these areas. Therefore, the aim of our study was to investigate the osteoconductive properties of pleiotrophin. We wanted to determine if pleiotrophin coating facilitates cellular adhesion and furthermore if this has any effect on hMSCs derived bone formation in an animal model. The results showed a dose dependent response of cellular adhesion in fibronectin samples, and cellular adhesion was facilitated with increasing pleiotrophin concentrations. Histologic findings taken after 5 weeks implantation in SCID mouse showed no presence of bone formation with only a dense fibrous connective tissue. Possible explanations for the results of the osteogenesis assay include inappropriate cell loading.

치주인대세포 및 치은섬유아세포의 증식능에 대한 Epidermal growth factor의 영향 (The Effect of EGF on Proliferation Rate of the Human Periodontal Ligament Cells and Human Gingival Fibroblasts)

  • 김선우;이재목;서조영
    • Journal of Periodontal and Implant Science
    • /
    • 제26권4호
    • /
    • pp.841-858
    • /
    • 1996
  • Epidermal growth factor(EGF) is one of polypeptide growth factors. EGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purposes of this study is to evaluate the effects of EGF on the human periodontal ligament cells and human gingival fibroblast cells that promote regeneration of periodntal tissue. The mitogenic effects of epidermal growth factor on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of 5-Bromo-2'-deoxy-uridine into DNA of the cells in a dose dependent manner. The prepared cells were the primary cultured gingival fibroblast and periodontal ligament cells from humans, the fourth or sixth subpassages were used in the experiments. Cells were seeded in DMEM containing 10% FBS. 1, 10, 50, 100, $200{\eta}g/ml$ and epidermal growth factor were added to the quiescent cells for 24 hours, 48 hours and 72 hours. They were labeled with $10\{mu}l/200{\mu}l$ 5-Bromo-2'-deoxy-uridine for the last 6 hours of each culture. The results of the five determinants were presented as mean and S.D.. The results were as follows : The DNA synthetic activity of human gingival fibroblasts were increased dose dependently by epidermal growth factor at 24 hours, 48 hours and 72 hours. The mitogenic effects were similar at the 24 and 48 hours of epidermal growth factor, but the DNA synthetic activity of human gingival fibroblasts generally decreased at 72 hours. The DNA synthetic activity of human periodontal ligament cells were increased dose dependently by epidermal growth factor at 24 hours but the DNA synthetic activity decreased at $200{\eta}g/ml$ of each hour. Generally the maximum mitogenic effects were observed at the 48 hours application of epidermal growth factor. The DNA synthetic activity of human periodontal ligament cells generally decreased lower at 24, 72 hours than at 48 hours the application of epidermal growth factor. In the comparison of DNA synthetic activity between human gingival fibroblasts and human periodontal ligament cells, human periodontal ligament cells had slightly higher proliferation activity than human gingival fibroblasts for a longer time at the high dosage of the epidermal growth factor. In conclusion, epidermal growth factor have important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, and thus may be useful for clinical applications in periodontal regenerative procedures.

  • PDF

후박 및 은행잎 추출물의 향균, 향염 및 세포활성도에 미치는 영향 (BIOLOGICAL EFFECT OF MAGNOLIA AND GINKGO BILOBA EXTRACT TO THE ANTIMICROBIAL, ANTIINFLAMMATORY AND CELLULAR ACTIVITY)

  • 정종평;구영;배기환
    • Journal of Periodontal and Implant Science
    • /
    • 제25권3호
    • /
    • pp.478-486
    • /
    • 1995
  • Periodontal therapy for treatment of periodontitis involves the elimination of bacterial plaque and elimination of the anatomic defects by regenerative procedure. The purpose of this study was to evaluate on the biological effect of magnolia and Ginkgo biloba extract to the antimicrobial, antiinflammatory and cellular activity. Antimicrobial assay was performed with the diffusion method of the extract by measuring of growth inhibitory zone of B. cereus from blood agar plate. Effect of the extract to cellular activity of gingival fibroblast were examined using MTT method and measured the result with optical density on 570nm by ELISA reader. Inhibitory effects of $PGE_2$ production from gingival fibroblast was performed with the addition of $IL-l{\beta}$ and the extract to the well and examined to the product of $PGE_2$ from cell by ELISA reader. In vivo anti-inflammatory effect was performed with injection examined with clinically and histologically for their extent of mecrosis and inflammation. Antimicrobial activity of Magnolia extract showed significantly higher activity than that of control. However, GBE did not showed significant activity to compare with control, and mixture of Magnolia and GBE extract showed significantly higher activity than that of control. The effect of cellular activity to gingival fibroblast showed no significant differences of between control and Magnolia extract. However, GBE showed significantly higher rate of cellular activity to compare with control and even to PDGF-BB, and also showed same degree of cellular activity even though mixed with Magnolia extract. The inhibitory effect of $PGE_2$ production showed significantly reduction of $PGE_2$ production to compare with control, but its inhibitory effect was not much strong to compare with Indomethacin. In vivo, antiinflammatory effect of Magnolia extract to P. gingivalis injection of Hamster buccal check showed significantly reduction of inflammatory cell infiltration and tissue necrosis, but GBE showed no effect on the inhibition of inflammatory process. These results suggested that Magnolia and GBE extract possessed different kind of biological activity and also can be compensated on their activity with each other for elimination of bacterial plaque and anatonical defect.

  • PDF