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Optimization of the extraction process of high levels of chlorogenic acid and ginsenosides from short-term hydroponic-cultured ginseng and evaluation of the extract for the prevention of atopic dermatitis

  • Lee, Tae Kyung;Lee, Ji Yun;Cho, Yeon-Jin;Kim, Jong-Eun;Kim, Seo Yeong;Park, Jung Han Yoon;Yang, Hee;Lee, Ki Won
    • Journal of Ginseng Research
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    • v.46 no.3
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    • pp.367-375
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    • 2022
  • Background: Short-term hydroponic-cultured ginseng (sHCG), which is 1-year-old ginseng seedlings cultivated for 4 weeks in a hydroponic system, is a functional food item with several biological effects. However, the optimal extraction conditions for sHCG, and the bioactivity of its extracts, have not been evaluated. Methods: Chlorogenic acid (CGA) and ginsenoside contents were evaluated in sHCG, white ginseng (WG), and red ginseng (RG) using high-performance liquid chromatography. Response surface methodology (RSM) was used to optimize the extraction conditions (temperature and ethanol concentration) to maximize the yield of dry matter, CGA, and four ginsenosides (Re, Rg1, Rb1, and Rd) from sHCG. The optimal extraction conditions were applied to pilot-scale production of sHCG extracts. The expression levels of tumor necrosis factor (TNF)-α/interferon (IFN)-γ-induced thymic and activation-regulated chemokines (TARC/CCL17) were measured after treatment with sHCG, WG, and RG extracts, and the effects of their bioactive compounds (CGA and four ginsenosides) on human skin keratinocytes (HaCaTs) were evaluated. Results: CGA and four ginsenosides, which are bioactive compounds of sHCG, significantly inhibited TNF-α/IFN-γ-induced TARC/CCL17 expression. The optimal sHCG extraction conditions predicted by the RSM models were 80 ℃ and 60% ethanol (v/v). The sHCG extracts produced at the pilot scale under optimal conditions greatly alleviated TNF-α/IFN-γ-induced TARC/CCL17 production compared with WG and RG extracts. Conclusions: Pesticide-free sHCG extracts, which contain high levels of CGA and the ginsenosides Re, Rg1, Rb1, and Rd as bioactive compounds, may have therapeutic potential for atopic diseases.

Analysis of Animal Usage of Eco-bridge and Ecoduct Using an Infrared CCTV at the Baekdudaegan Mountain Range, Korea (적외선 CCTV를 활용한 백두대간 육교형 생태통로와 터널형 생태통로의 동물이용현황 분석)

  • Cho, Hye-Jin
    • Ecology and Resilient Infrastructure
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    • v.3 no.3
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    • pp.177-181
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    • 2016
  • In order to prevent the fragmentation of animal habitat due to road construction, the most widely applied solution is building animal passes worldwide. In Korea, animal passes were introduced in the early 2000s, and through trial and error, the national guidelines for them and their design standards were published in 2010. These were criticized by politicians because of their relative inefficiency considering their high construction cost and their lack of animal usage. This study investigated the extent to which animals used the facilities. For this study, two types of animal passes, eco-bridges and ecoducts, were considered and the test sites were chosen from the Baekdu Mountains. The animal usage data was captured using infra-red CCTV cameras. The results showed that various types of animals used eco-bridges and ecoducts. Interestingly various types of birds were captured by cameras and endangered animals were also in them. The season, weather, and their surrounded vegetation also had effects on their usages. The infrared CCTV allowed detailed captures of animals but the electricity shortage was one disadvantage. During the last decades, a number of eco-bridges were constructed throughout the country and now we need to focus on their monitoring and maintenance for their successful efficiency and application.

Development of the Standard Blood Inventory Level Decision Rule in Hospitals (병원의 표준 혈액재고량 산출식 개발)

  • Kim, Byoung-Yik
    • Journal of Preventive Medicine and Public Health
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    • v.21 no.1 s.23
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    • pp.195-206
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    • 1988
  • Two major issues of the blood bank management are quality assurance and inventory control. Recently, in Korea blood donation has gained popularity increasingly to allow considerable improvement of the quality assurance with respect to blood collection, transportation, storage, component preparation skills and hematological tests. Nevertheless the inventory control, the other issue of blood bank management, has been neglected so far. For the supply of blood by donation barely meets the demand, the blood bank policy on the inventory control has been 'the more the better.' The shortage itself by no means unnecessitate inventory control. In fact, in spite of shortage, no small amount of blood is outdated. The efficient blood inventory control makes it possible to economize the blood usage in the practice of state-of-the-art medical care. For the efficient blood inventory control in Korean hospitals, this tudy is to develop formulae forecasting the standard blood inventory level and suggest a set of policies improving the blood inventory control. For this study informations of $A^+$ whole bloods and packed cells inventory control were collected from a University Hospital and the Central Blood Bank of the Korean Red Cross. Using this informations, 1,461 daily blood inventory records were formulated.48 varieties of blood inventory control environment were identified on the basis of selected combinations of 4 inventory control variables-crossmatch, transfusion, inhospital donation and age of bloods from external supply. In order to decide the optimal blood inventory level for each environment, simulation models were designed to calculate the measures of performance of each environment. After the decision of 48 optimal blood inventory levels, stepwise multiple regression analysis was started where the independent variables were 4 inventory control variables and the dependent variable was optimal inventory level of each environment. Finally the standard blood inventory level decision rule was developed using the backward elimination procedure to select the best regression equation. And the effective alternatives of the issuing policy and crossmatch release period were suggested according to the measures of performance under the condition of the standard blood inventory level. The results of this study' were as follows ; 1. The formulae to calculate the standard blood inventory level($S^*$)was $S^*=2.8617X(d)^{0.9342}$ where d is the mean daily crossmatch(demand) for a blood type. 2. The measures of performace - outdate rate, average period of storage, mean age of transfused bloods, and mean daily available inventory level - were improved after maintenance of the standard inventory level in comparison with the present system. 3. Issuing policy of First In-First Out(FIFO) decreased the outdate rate, while Last In-First Out(LIFO) decreased the mean age of transfused bloods. The decrease of the crossmatch release period reduced the outdate rate and the mean age of transfused bloods.

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Changes in Riboflavin and Ascorbic Acid Content during Ripening of Kimchi (절임조건별(條件別) 배추에 의한 김치의 숙성중(熟成中) Riboflavin과 Ascorbic Acid의 함량변화(含量變化))

  • Rhie, Seung-Gyo;Kim, Hwa-Za
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.2
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    • pp.131-135
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    • 1984
  • For the convenience of transportation and improvement of the utility of raw chinese cabbage during harvest season, the condition of pretreatment for the raw chinese cabbage with pickle has been examined by preserving for one to ten days. The changes in the content of riboflavin and ascorbic acid in kimchi (pickled-seasoned chinese cabbage), which was manufactured by using the pickled cabbage as a main raw material, along with ginger, garlic, red pepper powder, rad-dish and fermented shrimp as seasonings, during ripening were investigated with specially referred to palatability. The yield of the pickled cabbage compared to the raw cabbage after treating with pickle was 62 to 65% in volume and 15 to 42% in weight. After 10 days of pickling of the chinese cabbage, one-third of total ascorbic acid was diminished but no change in riboflavin content was observed. During ripening of kimchi, riboflavin content in kimchi processed with the pickled cabbage has not been changed compared to the content in convention- ally made kimchi, however, slight decrease in ascorbic acid content in the kimchi processed with the pickled cabbage was as certained. The result of organoleptic test showed that the kimchi processed with the pickled cabbage became inferior to the conventionally made kimchi with the days of ripening.

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Study on Time-of-day Operation of Pedestrian Signal Based on Residual Pedestrians (잔류보행기반 시간대별 보행신호 운영기법 연구)

  • Chae, HeeChul;Eom, Daelyoung;Yun, Ilsoo
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.21 no.4
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    • pp.1-17
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    • 2022
  • As pedestrian deaths account for a high proportion of traffic accident deaths in Korea, interest in pedestrian safety is growing. In particular, it is necessary to develop various pedestrian-centered traffic signal operation techniques to improve the pedestrian environment at signal intersections. Therefore, in this study, a method for time-of-day operating a pedestrian signal based on residual pedestrians was studied. To this end, the pedestrian signal operation technique in response to the pedestrian demand, which is operated by extending the pedestrian signal time only during the time when the pedestrian demand and the number of remaining pedestrians increase, was applied to the field. The difference in safety according to the application of the new pedestrian signal operation technique was statistically analyzed. As a result of the analysis, the residual pedestrian rate decreased by 20% (3.3 people) before application and 8% (1.4 people) after application, and the residual pedestrian rate in the crosswalk at the time of red signal decreased by 12% (1.9 people), And it was analyzed that the position of the residual pedestrian decreased by 3.3m from 5.2m before application to 1.9m after application.

Characteristic of Environmental Factors Related to Outbreak and Decline of Cochlodinium polykrikoides Bloom in the southeast coastal waters of Korea, 2007 (2007년 남해동부해역의 Cochlodinium polykrikoides 적조 발생과 소멸에 미치는 환경 특성)

  • Lim, Weol-Ae;Lee, Young-Sik;Lee, Sam-Geun
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.4
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    • pp.325-332
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    • 2008
  • To characterize the initiation, propagation and termination of Cochlodinium polykrikoides blooms in the southeast coastal waters of Korea, 2007, we have analyzed the data set of phytoplankton composition, physical and chemical water properties, and meterological data. The development of C. polykrikoides bloom in 2007 can be summarized in three steps. The first stage from middle of July to end of August was characterized by an unusually persistent and strong southerly wind. C. polykrikoides blooms established already by the strong wind in the middle of south coastal waters were advected intermittently into the study area. Accordingly, highly variable cell densities of C. polykrikoides were observed. At the second stage a favorable growing conditions for C. polykrikoides was developed, which was directed by changes in wind direction from south to northeast and thus enhanced transportation of offshore waters into inshore (August 8 to 30). C. polykrikoides bloom occurred through typical mechanism and showed high cell density. The last stage was represented by disappearance of C. polykrikoides. Typoon 'Nari' carrying heavy rain brought an unfavorable habitat to C. polykrikoides. Low saline condition formed in coastal water due to typoon effects continuously drove the dominant species to diatoms and flagellates which were prevailing over C. polykrikoides in this circumstances(from the middle of September). These stepwise processes could be the causative mechanism of the extraordinarily persistent C. polykrikoides bloom observed in southeast coastal water of Korea, 2007.

Retrieving Volcanic Ash Information Using COMS Satellite (MI) and Landsat-8 (OLI, TIRS) Satellite Imagery: A Case Study of Sakurajima Volcano (천리안 위성영상(MI)과 Landsat-8 위성영상(OLI, TIRS)을 이용한 화산재 정보 산출: 사쿠라지마 화산의 사례연구)

  • Choi, Yoon-Ho;Lee, Won-Jin;Park, Sun-Cheon;Sun, Jongsun;Lee, Duk Kee
    • Korean Journal of Remote Sensing
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    • v.33 no.5_1
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    • pp.587-598
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    • 2017
  • Volcanic ash is a fine particle smaller than 2 mm in diameters. It falls after the volcanic eruption and causes various damages to transportation, manufacturing industry and respiration of living things. Therefore diffusion information of volcanic ash is highly significant for preventing the damages from it. It is advantageous to utilize satellites for observing the widely diffusing volcanic ash. In this study volcanic ash diffusion information about two eruptions of Mt. Sakurajima were calculated using the geostationary satellite, Communication, Ocean and Meteorological Satellite (COMS) Meteorological Imager (MI) and polar-orbiting satellite, Landsat-8 Operational Land Imager (OLI) and the Thermal InfraRed Sensor (TIRS). The direction and velocity of volcanic ash diffusion were analyzed by extracting the volcanic ash pixels from COMS-MI images and the height was retrieved by adjusting the shadow method to Landsat-8 images. In comparison between the results of this study and those of Volcanic Ash Advisories center (VAAC), the volcanic ash tend to diffuse the same direction in both case. However, the diffusion velocity was about four times slower than VAAC information. Moreover, VAAC only provide an ash height while our study produced a variety of height information with respect to ash diffusion. The reason for different results is measured location. In case of VAAC, they produced approximate ash information around volcano crater to rapid response, while we conducted an analysis of the ash diffusion whole area using ash observed images. It is important to measure ash diffusion when large-scale eruption occurs around the Korean peninsula. In this study, it can be used to produce various ash information about the ash diffusion area using different characteristics satellite images.

Study on the Technological System of the Cooperative Cultivation of Paddy Rice in Korea (수도집단재배의 기술체계에 관한 연구)

  • Min-Shin Cho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.8 no.1
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    • pp.129-177
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    • 1970
  • For the purpose of establishing the systematized technical scheme of the cooperative rice cultivation which has most significant impact to improve rice productivity and the farm management, the author have studied the cultivation practices, and the variation of rice growth and yield between the cooperative rice cultivation and the individual rice cultivation at random selected 18 paddy fields. The author also have investigated through comparative method on the cultivation practices, management, organization and operation scheme of the two different rice cultivation methods at 460 paddy fields. The economic feasibility has been ana lysed and added in this report. The results obtained from this study are summarized as follows; 1. In the nursery, the average amount of fertilizer application, especially, phosphate and potassium, and the frequency of chemicals spray for the disease, insect and pest control at the cooperative rice cultivation are significantly higher than those of the individual rice cultivation. 2. The cultivation techniques of the cooperative rice farming after the transplanting can be characterized by a) the earlier transplanting of rice, b) the denser hills per unit area and the lesser number of seedlings per hill, c) the application of larger quantities of fertilizer including nitrogen, phosphate and potassium, d) more divided application of fertilizers, split doses of the nitrogen and potassium, e) the increased frequencies of the chemicals spray for the prevention of disease, insect and pest damages. 3. The rate of lodging in the cooperative rice cultivation was slightly higher than that of the individual rice cultivation, however, the losses of rice yield owing to the occurrence of rice stem borer and grass leaf roller in the cooperative rice cultivation were lower than that of the individual rice cultivation. 4. The culm length, panicle length, straw weight and grain-straw ratio are respectively higher at the cooperative rice cultivation, moreover, the higher variation of the above factors due to different localities of the paddy fields found at the individual rice cultivation. 5. The number of panicles, number of flowers per panicle and the weight of 1, 000 grains, those contributing components to the rice yield were significantly greater in the cooperative rice cultivation, however, not clear difference in the maturing rate was observed. The variation coefficient of the yield component in the cooperative cultivation showed lower than that or the individual rice cultivation. 6. The average yield of brown rice per 10 are in the cooperative rice cultivation obtained 459.0 kilograms while that of the individual rice cultivation brought 374.8 kilograms. The yield of brown rice in the cooperative rice cultivation increased 84.2 kilogram per 10 are over the individual rice cultivation. With lower variation coefficient of the brown rice yield in the cooperative rice cultivation, it can be said that uniformed higher yield could be obtained through the cooperative rice cultivation. 7. Highly significant positive correlations shown between the seeding date and the number of flowers per panicle, the chemical spray and the number of flowers per panicle, the transplanting date and the number of flowers per panicle, phosphate application and yield, potassium application and maturing rate, the split application of fertilizers and yield. Whilst the significant negative correlation was shown between the transplanting date and the maturing rate 8. The results of investigation from 480 paddy fields obtained through comparative method on the following items are identical in general with those obtained at 18 paddy fields: Application of fertilizers, chemical spray for the control of disease, insects and pests both in the nursery and the paddy field, transplanting date, transplanting density, split application of fertilizers and yield n the paddy fields. a) The number of rice varieties used in the cooperative rice cultivation were 13 varieties while the individual rice cultivation used 47 varieties. b) The cooperative rice cultivation has more successfully adopted improved cultivation techniques such as the practice of seed disinfection, adoption of recommended seeding amount, fall ploughing, application of red soil, introduction of power tillers, the rectangular-type transplanting, midsummer drainage and the periodical irrigation. 9. The following results were also obtained from the same investigation and they are: a) In the cooperative rice cultivation, the greater part of the important practices have been carried out through cooperative operation including seed disinfection, ploughing, application of red soil and compost, the control of disease, insects and pests, harvest, threshing and transportation of the products. b) The labor input to the nursery bed and water control in the cooperative rice cultivation was less than that of the individual rice cultivation while the higher rate of labor input was resulted in the red soil and compost application. 10. From the investigation on the organization and operation scheme of the cooperative rice cultivation, the following results were obtained: a) The size of cooperative rice cultivation farm was varied from. 3 ha to 7 ha and 5 ha farm. occupied 55.9 percent of the total farms. And a single cooperative farm was consisted of 10 to 20 plots of paddies. b) The educational back ground of the staff members involved in the cooperative rice cultivation was superior than that of the individual rice cultivation. c) All of the farmers who participated to the questionaires have responded that the cooperative rice cultivation could promise the increased rice yield mainly through the introduction of the improved method of fertilizer application and the effective control of diseases, insects and pests damages. And the majority of farmers were also in the opinion that preparation of the materials and labor input can be timely carried out and the labor requirement for the rice cultivation possibly be saved through the cooperative rice cultivation. d) The farmers who have expressed their wishes to continue and to make further development of the cooperative rice cultivation was 74.5 percent of total farmers participated to the questionaires. 11. From the analysis of economical feasibility on the two different methods of cultivation, the following results were obtained: a) The value of operation cost for the compost, chemical fertilizers, agricultural chemicals and labor input in the cooperative rice cultivation was respectively higher by 335 won, 199 won, 288 won and 303 won over the individual rice cultivation. However, the other production costs showed no distinct differences between the two cultivation methods. b) Although the total value of expenses for the fertilizers, agricultural chemicals, labor input and etc. in the cooperative rice cultivation were approximately doubled to the amount of the individual rice cultivation, the net income, substracted operation costs from the gross income, was obtained 24, 302 won in the cooperative rice cultivation and 20, 168 won was obtained from the individual rice cultivation. Thereby, it can be said that net income from the cooperative rice cultivation increased 4, 134 won over the individual rice cultivation. It was revealed in this study that the cooperative rice cultivation has not only contributed to increment of the farm income through higher yield but also showed as an effective means to introduce highly improved cultivation techniques to the farmers. It may also be concluded, therefore, the cooperative rice cultivation shall continuously renovate the rice production process of the farmers.

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