• The Plant Pathology Journal
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• 제31권3호
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• pp.259-268
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• 2015

This study investigated the chemical characteristics and microbial population during incubation of four kinds of aerated compost teas based on oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR). It aimed to determine the effects of the aerated compost tea (ACT) based on MOVR on the growth promotion of red leaf lettuce, soybean and sweet corn. Findings showed that the pH level and EC of the compost tea slightly increased based on the incubation time except for rice straw compost tea. All compost teas except for oriental medicinal herbs and rice straw compost tea contained more ${NO^-}_3-N$ than ${NH^+}_4-N$. Plate counts of bacteria and fungi were significantly higher than the initial compost in ACT. Microbial communities of all ACT were predominantly bacteria. The dominant bacterial genera were analyzed as Bacillus (63.0%), Ochrobactrum (13.0%), Spingomonas (6.0%) and uncultured bacterium (4.0%) by 16S rDNA analysis. The effect of four concentrations, 0.1%, 0.2%, 0.4% and 0.8% MOVR on the growth of red leaf lettuce, soybean and sweet corn was also studied in the greenhouse. The red leaf lettuce with 0.4% MOVR had the most effective concentration on growth parameters in foliage part. However, 0.8% MOVR significantly promoted the growth of root and shoot of both soybean and sweet corn. The soybean treated with higher MOVR concentration was more effective in increasing the root nodule formation by 7.25 times than in the lower MOVR concentrations Results indicated that ACT could be used as liquid nutrient fertilizer with active microorganisms for culture of variable crops under organic farming condition.

• 한국미생물·생명공학회지
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• 제14권4호
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• pp.279-283
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• 1986

홍삼제품 제조 과정중의 부산물인 홍삼박의 활용 가능성을 조사하기 위하여 여러 가지 담자균의 배양에 홍삼박 추출물을 첨가하여 배양한 후 균체 증식량, 단백질 및 amino acid함량 등을 조사하였으며 그 결과를 보고하는 바이다. 수집 된 담자균 중 G. lucidum과 P. ostreatus가 액체배양에서 균체증식이 양호한 균주로 선발되었으며, 홍삼박 추출물을 0.2％ 첨가하였을 때 균체증식량이 가장 많았으며 단백질 함량은 0.5％ 첨가하였을 때 l-3％가 증가하였다. Amino acid함량은 인삼박 추출물을 0.2％ 첨가하였을 때는 거의 변화가 없었으나 0.5％ 첨가하였을 때에는 G. lucidum, P. ostreatus 모두 상당히 증가하였으며 그 증가폭 중 cysteine이 가장 컸다.

• 한국균학회지
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• 제40권4호
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• pp.258-265
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• 2012

국내 고추 재배지에서 고추탄저병균인 C. gloeosporioides에 대해 균사생장이나 포자발아 억제력이 우수한 균주를 선발하였다. 선발된 균주의 생리 생화학적특성과 MicroLog 방법을 이용하여 동정한 균주는 Paenibacillus sp. IUB225-08로 명명되었다. 항진균물질의 최적 생산을 위한 배양 조건은 pH 7.0, $25^{\circ}C$에서 60시간인 것으로 나타났다. Paenibacillus sp. IUB225-08를 배양한 액체배지에 부탄올을 첨가하여 분획한 물질과 시판 농약의 C. gloeosporioides에 대한 균사생장과 포자 발아억제 효과를 조사한 결과 부탄올 분획물이 시판 농약에 비해 고추탄저병균의 균사생장과 포자발아를 크게 억제하는 것으로 나타났다. 또한 고추열매와 고추종자에 C. gloeosporioides 포자와 부탄올로 분획한 물질을 동시에 접종한 결과 부탄올 분획물이 고추와 고추종자의 병 발생을 억제하는 것으로 나타났다. 따라서 본 연구를 통해 Paenibacillus sp. IUB225-08가 생산하는 항진균 물질은 고추탄저병의 생물적 방제제로 사용될 수 있는 잠재력이 있다고 판단되었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권3호
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• pp.166-170
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• 2004

Gluconobacter oxydans that produces the cellulose was isolated. In order to confirm the chemical features of cellulose, various spectrophtometeric analysis were carried out using electron microscopy, X-ray diffractogram, and CP/MAS $\^$13/C NMR. The purified cellulose was found to be identical to that of Acetobacter xylinum. For effective production of cellulose, the various carbon and nitrogen sources, mixture of calcium and magnesium ions, and biotin concentration were investigated in flask cultures. Among the various carbon sources, glucose and sucrose were found to be best for the production of cellulose, with maximum concentration of 2.41 g/L obtained when a mixture of 10 g/L of each glucose and sucrose were used. With regard to the nitrogen sources, when 20 g/L of yeast extract was used, the maximum concentration of bacterial cellulose was reached. The concentration of cellulose was increased with mixture of 2 mM of each Ca$\^$2+/ and Mg$\^$2+/. The optimum biotin concentration for the production of cellulose was in the range of 15 to 20mg/L. At higher biotin concentration (25-35mg/L). the bacterial cellulose production was lower.