• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.40-54
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• 2006

It's not easy to detect the high quality and functional compounds for control quality of food materials. The electronic nose was an instrument, which comprised of an array of electronic chemical sensors with partial specificity and an appropriate pattern recognition system, capable of recognizing simple or complex odors. It can conduct fast analysis and provide simple and straightforward results and is best suited for quality control and process monitoring in the field of functional foods. Numbers of applications of an electronic nose in the functional food industry include discrimination of habitats for medicinal food materials, monitoring storage process, lipid oxidation, and quality control of food and/or processing with principal component analysis, neural network analysis and the electronic nose based on GC-SAW sensor. The electronic nose would be possibly useful for a wide variety of quality control in the functional food and plant cultivation when correlating traditional analytical instrumental data with sensory evaluation results or electronic nose data.

• Biomolecules & Therapeutics
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• 제21권6호
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• pp.423-434
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• 2013

The adoption of oligonucleotide aptamer is well on the rise, serving an ever increasing demand for versatility in biomedical field. Through the SELEX (Systematic Evolution of Ligands by EXponential enrichment), aptamer that can bind to specific target with high affinity and specificity can be obtained. Aptamers are single-stranded nucleic acid molecules that can fold into complex three-dimensional structures, forming binding pockets and clefts for the specific recognition and tight binding of any given molecular target. Recently, aptamers have attracted much attention because they not only have all of the advantages of antibodies, but also have unique merits such as thermal stability, ease of synthesis, reversibility, and little immunogenicity. The advent of novel technologies is revolutionizing aptamer applications. Aptamers can be easily modified by various chemical reactions to introduce functional groups and/or nucleotide extensions. They can also be conjugated to therapeutic molecules such as drugs, drug containing carriers, toxins, or photosensitizers. Here, we discuss new SELEX strategies and stabilization methods as well as applications in drug delivery and molecular imaging.

• 약학회지
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• 제47권3호
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• pp.176-179
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• 2003

A library of unlimited number of novel lectins with diverse specificities has been previously generated by randomly mutating the carbohydrate-recognition domain of Maackia amurensis hemagglutinin (MAH). To establish the experimental environment capable of selecting high affinity mutant lectins in E. coli, phage display system was adapted. Carbohydrate binding capacity of two phagemid vectors, pComb3 and pComb8 displaying wild-type MAH lectin was assessed. Specific bindings of pComb3 and pComb8 phages expressing w.t. MAH to affinity-purified polyclonal anti-MAH antibody and to glycophorin was demonstrated. Both phages also showed strong hemagglutinating activity to intact but not sialidase-treated human erythrocytes, which is consistent to the specificity of native MAH. Taken together, two different phage display vectors successfully allowed the expression of active MAH as a fusion protein on the surface of bacteriophage, which will lead to preparation of unique plant lectins with high affinity toward a variety of carbohydrate chains.

• 한국식품과학회지
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• 제37권6호
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• pp.1048-1064
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• 2005

Detection of specific compounds influencing food flavor quality is not easy. Electronic nose, comprised of electronic chemical sensors with partial specificity and appropriate pattern recognition system, is capable of recognizing simple and complex volatiles. It provides fast analysis with simple and straightforward results and is best suited for quality control and process monitoring of flavor in food industry. This review examines application of electronic nose in food analysis with brief explanation of its principle. Characteristics of different sensors and sensor drift. and solutions to related problems are reviewed. Applications of electronic nose in food industry include monitoring of fermentation process and lipid oxidation, prediction of shelf life, identification of irradiated volatile compounds, discrimination of food material origin, and quality control of food and processing by principal component analysis and neural network analysis. Electronic nose could be useful for quality control in food industry when correlating analytical instrumental data with sensory evaluation results.

• Journal of Korean Neurosurgical Society
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• 제65권3호
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• pp.397-407
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• 2022

Abusive head trauma (AHT) is the most common and serious form of child abuse and a leading cause of traumatic death in infants and young children. The biomechanics of head injuries include violent shaking, blunt impact, or a combination of both. Neuroimaging plays an important role in recognizing and distinguishing abusive injuries from lesions from accidental trauma or other causes, because clinical presentation and medical history are often nonspecific and ambiguous in this age group. Understanding common imaging features of AHT can increase recognition with high specificity for AHT. In this review, we discuss the biomechanics of AHT, imaging features of AHT, and other conditions that mimic AHT.

• BMB Reports
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• 제40권5호
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• pp.783-790
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• 2007

Receptor binding plays an important role in determining host specificity of the Bacillus thuringiensis Cry $\delta$-endotoxins. Mutations in domains II and III have suggested the participation of certain residues in receptor recognition and insect specificity. In the present study, we expressed the cloned domain II-III fragment of Cry4Ba and examined its binding characteristics to mosquito-larval midgut proteins. The 43-kDa Cry4Ba-domain II-III protein over-expressed in Escherichia coli as inclusion bodies was only soluble when carbonate buffer, pH 10.0 was supplemented with 4M urea. After renaturation via stepwise dialysis and subsequent purification, the refolded domain II-III protein, which specifically reacts with anti Cry4Ba-domain III monoclonal antibody, predominantly exists as a $\beta$-sheet structure determined by circular dichroism spectroscopy. In vitro binding analysis to both histological midgut tissue sections and brush border membrane proteins prepared from susceptible Aedes aegypti mosquito-larvae revealed that the isolated Cry4Ba-domain II-III protein showed binding functionality comparable to the 65-kDa full-length active toxin. Altogether, the data present the 43-kDa Cry4Ba fragment comprising domains II and III that was produced in isolation was able to retain its receptor-binding characteristics to the target larval midgut proteins.

• 한국융합학회논문지
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• 제13권3호
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• pp.11-22
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• 2022

최근 교육 분야에서 다양한 인공지능 기술을 활용한 연구와 개발이 이뤄지고 있다. 인공지능을 활용한 교육 중 특히 대화형 에이전트는 시간과 공간의 제약을 받지 않고 음성인식, 번역과 같은 다양한 인공지능 기술과 결합해 더 효과적인 언어 학습을 가능하게 한다. 본 논문은 상용화된 교육용 플랫폼 중 이용자 수가 많고 영어 학습을 위한 대화형 에이전트가 활용된 플랫폼에 대한 동향 분석을 진행하였다. 동향 분석을 통해 현재 상용화된 교육용 플랫폼의 대화형 에이전트는 여러 한계점과 문제점이 존재했다. 구체적인 문제점과 한계점 분석을 위해 사전 학습된 최신 대용량 대화 모델과 비교 실험을 진행하였고, 실험 방법으로 대화형 에이전트의 대답이 사람과 비슷한지를 평가하는 Sensibleness and Specificity Average (SSA) 휴먼 평가를 진행하였다. 실험 내용을 바탕으로, 효과적인 학습을 위해 개선방안으로 대용량 파라미터로 학습된 대화 모델, 교육 데이터, 정보 검색 기능의 필요성을 제안했다.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제23권2호
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• pp.69-75
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• 2012

Objectives : The current study was conducted in order to investigate teachers' recognition of school bullying using a nationwide database of adolescents in middle and high school in Korea. Methods : Students in the 7th to 12th grades at 23 secondary schools participated in the current study during the fall of 2009. Subjects completed the self-report form of the Adolescent Mental Health and Problem Behavior Screening Questionnaire-II (AMPQ-II) and Symptom Checklist-90 Revised (SCL-90-R). In addition, relevant teachers used the teachers' rating scale of the AMPQ-II to report their students' status. Differences in the number of bullied students between teachers' recognition and students' report were explored. Results : A total of 2270 subjects provided relevant responses to the questionnaire. While the one-month prevalence of victimization according to students' self-reports was 28.9%, the recognized prevalence by teachers was only 10.6%. For prediction of the presence of school bullying according to students' self reports on the AMPQ-II, item 7 of the teachers' report on the AMPQ-II showed a sensitivity of 16%, a specificity of 92%, a positive predictability of 44%, a negative predictability of 72%, a false positive rate of 8%, a false negative rate of 84%, and an accuracy of 69%, respectively. No significant differences in subscores of students' self reports of the AMPQ-II and SCL-90-R were observed between bullied students who were recognized by teachers and those who were not recognized. In stepwise discriminant analysis, classification of teachers' item 2 and item 7 on the AMPQ-II with respect to school bullying according to students' reports showed an accuracy of 63.4%. Using this model, 75.2% of non-victimized subjects were classified correctly, while only 35.2% of victimized subjects were classified correctly. Conclusion : Despite the high prevalence in Korea, teachers' recognition of school violence among their students remains low. Pre-professional and continuing education to improve teachers' understanding of school bullying and knowledge of effective classroom-based prevention activities should be encouraged.

• 디지털융복합연구
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• 제19권2호
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• pp.245-251
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• 2021

1,2인 가구가 꾸준하게 늘어나고 있는 추세에 비어 있는 시간대에 집을 방문하는 외부인이 누구인지 확인하고 싶은 요구가 증가하고 있다. 얼굴인식 기술은 많은 연구를 통해 여러 가지 모델이 제안되었는데 OpenCV의 Harr Cascade와 Dlib의 Hog가 대표적인 오픈소스 모델이다. 두 모델은 사용 환경에 따른 장단점을 가지고 있는데, 본 연구에서 초점을 둔 실내 현관 앞과 제한된 거리에서는 Dlib의 Hog가 강점을 가진다. 본 논문에서는 딥러닝 오픈 소스인 Dlib에 기반을 둔 얼굴인식 방문자 출입관리 시스템을 설계하고 구현하였다. 전체 시스템은 프론트 모듈과 서버모듈, 모바일모듈로 구성되며 세부적으로는 얼굴등록, 얼굴인식, 실시간 방문자 확인 및 원격제어, 동영상 저장 기능을 포함한다. 인터넷에서 공개된 사진을 이용하여 거리임계 값의 변화에 따른 정밀도, 특이도, 정확도를 구하고 선행연구 결과와 비교하였다. 실험 결과 구현된 시스템이 정상적으로 동작하는 것을 확인 하였으며 Dlib에서 보고한 것과 비슷한 결과를 보이는 것을 확인 하였다.

• Korean Chemical Engineering Research
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• 제48권5호
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• pp.574-582
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• 2010

본 연구는 기상확산 자기조립화기법으로 수정진동자 표면을 L-Penicillamine와 D-Penicillamine으로 수식하여 대표적인 키랄물질인 R-(-)-Mandelic acid와 S-(+)-Mandelic acid에 대한 키랄물질 인식과 측정 가능성을 검토한 것이다. 수정진동자의 전극 표면에 3차원 입체구조가 다른 Penicillamine의 이성질체를 기상확산법으로 수식한 결과를 QCM과 AFM을 이용하여 관찰하여, 수정진동자 표면위의 분자구조의 형성에서 상이한 구조를 가지게 된다는 것을 확인하였다. L, D-Penicillamine이 수식된 수정진동자를 이용하여 R-(-)-Mandelic acid와 S-(+)- Mandelic acid에 대한 감응성을 검토한 결과, L-Penicillamine이 수식된 수정진동자는 R-(-)-Mandelic acid와 S-(+)- Mandelic acid에 대하여 선택적 감응성이 없었으나 D-Penicillamine이 수식된 수정진동자는 R-(-)-Mandelic acid에 대하여 선택적 감응성을 나타냄을 알 수 있었다. 본 연구결과, 기상확산법을 이용하여 다양한 선택성 막의 배열이 가능함을 알 수 있었고, 이렇게 배열된 박막으로 수식된 QCM을 이용하여 Mandelic Acid 이성질체 혼합물에서 특정 광학 이성질체의 검출이 가능함을 확인할 수 있었다.