• Asian-Australasian Journal of Animal Sciences
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• 제19권4호
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• pp.554-562
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• 2006

Two lines of mice, M16 selected for rapid growth and a randomly selected control ICR as well as their reciprocal crosses were used to study the effects of genotype on whole-body energetics and intestinal responses to monensin. Six mice, eight weeks of age, from each line or reciprocal cross were assigned to one of two treatments, 1) drinking water containing 20 mmol/L monensin dissolved in 0.5% V/V ethanol, and 2) drinking water containing 0.5% V/V ethanol (control) for two weeks. After 11 days (age of 9 weeks and 4 days), whole-body $O_2$ consumption was measured. At the end of two weeks, jejunal $O_2$ consumption, intestinal tissue composition and histomorphometrics as well as the rate and efficiency of glucose absorption were estimated. In comparison with the control, monensin administration in drinking water resulted in less daily water intake (13.4 vs. 15.5 ml/mouse, p<0.01), less protein to DNA ratio of jejunal mucosa (5.41 vs. 6.01 mg/mg, p<0.05), lower villus width (88 vs. $100{\mu}m$, p<0.05), and less jejunal tissue $O_2$ consumption enhancement by alcohol (7.2 vs. 10.5%, p<0.01) in mice. Other than those changes, monensin had little (p>0.05) effect on variables measured in either line of mice or their reciprocal cross. In contrast, the M16 line, selected for rapid growth, as compared to the ICR controls or the reciprocal crosses, had less initial (pre-monensin treatment) whole-body $O_2$ consumption per gram of body weight (1.68 vs. $2.11-2.34{\mu}mol/min{\cdot}g$ BW, p<0.01) as compared to the ICR and reciprocal crosses. In addition, the M16 mice exhibited greater growth (412 vs. 137-210 mg/d, p<0.05), better feed efficiency (41.7 vs. 19.9-29.3 mg gain/g feed, p<0.05), shorter small intestines adjusted for fasted body weight (1.00 vs. 1.22-1.44 cm/g FBW, p<0.05), wider villi (109 vs. $87-93{\mu}m$, p<0.05), more mature height of enterocytes (28.8 vs. $24.4-25.1{\mu}m$, p<0.05) and a lower rate (91 vs. $133-145{\eta}mol\;glucose/min{\cdot}g$ jejunum, p<0.05) and less energetic efficiency (95 vs. $59-72{\eta}mol$ ATP expended/${\eta}mol$ glucose uptake, p<0.05) of glucose absorption compared to the ICR line and the reciprocal cross. Monensin had little (p>0.05) effect on whole-body $O_2$ consumption and jejunal function, whilst selection for rapid growth resulted in an apparent down-regulation of intestinal function. These data suggest that genetic selection for increased growth does not result in concomitant changes in intestinal function. This asynchrony in the selection for production traits and intestinal function may hinder full phenotypic expression of genotypic growth potential.

• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1625-1630
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• 2007

A simulation study was conducted to evaluate the effect of reciprocal cross on the detection and characterization of Mendelian QTL in $F_2$ QTL swine populations. Data were simulated under two different mating designs. In the one-way cross design, six $F_0$ grand sires of one breed and 30 $F_0$ grand dams of another breed generated 10 $F_1$ offspring per dam. Sixteen $F_1$ sires and 64 $F_1$ dams were randomly chosen to produce a total of 640 $F_2$ offspring. In the reciprocal design, three $F_0$ grand sires of A breed and 15 $F_0$ grand dams of B breed were mated to generate 10 $F_1$ offspring per dam. Eight $F_1$ sires and 32 $F_1$ dams were randomly chosen to produce 10 $F_2$ offspring per $F_1$ dam, for a total of 320 $F_2$ offspring. Another mating set comprised three $F_0$ grand sires of B breed and 15 $F_0$ grand dams of A breed to produce the same number of $F_1$ and $F_2$ offspring. A chromosome of 100 cM was simulated with large, medium or small QTL with fixed, similar, or different allele frequencies in parental breeds. Tests between Mendelian models allowed QTL to be characterized as fixed (LC QTL), or segregating at similar (HS QTL) or different (CB QTL) frequencies in parental breeds. When alternate breed alleles segregated in parental breeds, a greater proportion of QTL were classified as CB QTL and estimates of QTL effects for the CB QTL were more unbiased and precise in the reciprocal cross than in the one-way cross. This result suggests that reciprocal cross design allows better characterization of Mendelian QTL in terms of allele frequencies in parental breeds.

• 한국작물학회지
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• 제46권3호
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• pp.176-179
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• 2001

Source와 sink의 상대적 크기가 다른 고구마 품종간 이면접목을 통하여 생육에 미치는 고구마의 source와 sink의 상호관계를 분석함으로써, 고구마의 품종육성 및 재배법개선에 필요한 기초자료를 얻고자 수행한 결과를 요약하면 다음과 같다. 1 만장, 괴근수는 접목에 의하여 감소되었으며, 접목조합에 따라서 차이가 있었다. 2. 지상부의 생육은 접수, 지하부의 생육은 대목의 영향을 많이 받았다 3. 주만장과 괴근수에 대한 접목효과, 접수와 대목의 효과와 이들 간의 상호작용은 모두 유의성이 있었다. 4. 상저비율은 보통삽식＞자가접목＞이면접목 순으로 높았다.

• Journal of Pharmaceutical Investigation
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• 제12권2호
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• pp.31-36
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• 1982

The effect of some surfactants, such as tween 40, tween 80, span 80, and triethanolamine, on the absorption of lidocaine was studied using goldfish as a model of absorption kinetics. This model was believed that reciprocal overturn time and death time were the occurrence of biological effect versus drug absorption. The results were as follows; The threshold concentration of lidocaine was significantly reduced by the surfactants. Overturn time and death time of goldfish in the lidocaine solution were reduced by such surfactants as tween 40, tween 80, span 80 and triethanolamine, and more reduced with increasing concentration of surfactants except tween 40 and tween 80. The plots of reciprocal death time versus lidocaine concentration were linear relationship with a positive concentration intercept such as minimum effective concentration (0.2-0.8%). As results it is believed that these surfactants may form complexes with lidocaine or affect the goldfish membrane to make more permeable to lidocaine, which is adsorbed more rapidly.

• 한국작물학회지
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• 제37권4호
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• pp.339-346
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• 1992

콩 품종간의 상호접목을 통하여 지상부와 지하부의 능력을 비교하여 콩품종개량이나 재배법 개선의 기초자료를 제공하고자 황금콩, 팔달콩, 보광콩 및 장경콩을 공시하여 자가접목 및 품종간 상호접목을 하여 이식재배한 결과를 요약하면 다음과 같다. 1. 보광콩의 초장이 106.3-109.8cm로 가장 컸으며 팔달콩이 68.6-70.3cm로 제일 작았는데, 접목에 의하여 초장이 감소되기는 하였지만 대목의 영향은 인정되지 않았다. 2. 엽수는 팔달콩이 9.6-9.8매로 가장 적었으며, 장경콩이 14.2-15.3매로 제일 많았는데 처리간의 차이는 없었다. 3. 개화일수는 접목에 의하여 2-4일 지연되어 유의한 차이를 보였는데, 황금콩과 팔달콩은 자가접목보다는 상호접목에서 지연정도가 켰으나 보광콩과 장경콩은 상호접목보다는 자가접목에서 지연정도가 더 커서 품종간에 접목에 대한 반응이 다른 것으로 나타났다. 4. 부위별 건물중에 대한 접목의 영향도 품종간에 유의한 차이를 보여 황금콩과 팔달콩에서는 엽중에 대한 접목의 영향이 인정되었고, 보광콩과 장경콩의 상호접목에서도 장경콩이 보광콩의 부위별 건물중을 유의하게 감소시킨 것으로 나타났다. 5. 경태는 접목에 의하여 증가하였으며, 분지수는 감소하였고, 보광콩은 접목에 의하여 증중이 감소하였다. 6. 수양은 자가접목이 비접목에 비하여 11%, 상호접목은 자가접목에 비하여 14%그리고 상호접목은 비접목에 비하여 24% 감소하였으며, 경중은 수양과 같은 경향이었으나 접목에 의한 감소가 29-32%로 정도가 수량에 비하여 더 켰으나 자가접목과 상호접목간의 차이는 거의 없었다. 7 .수확지수는 접목에 의하여 황금콩과 장경콩은 1.4-6.1% 그리고 팔달콩과 보광콩은 4.4-8.6% 높아졌다. 8. 접목하였을 때의 수양이나 경중은 대목보다는 접수로 사용된 품종의 유전적 특성에 의하여 좌우되었으며, 품종과 특성에 따라서 접목에 대한 반응의 차이가 인정되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권12호
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• pp.1805-1811
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• 2007

A simulation study was conducted to evaluate the effect of reciprocal cross on the detection and characterization of parent-of-origin (POE) QTL in $F_2$ QTL populations. Data were simulated under two different mating designs. In the one-way cross design, six $F_0$ grand sires of one breed and 30 $F_0$ grand dams of another breed generated 10 $F_1$ offspring per dam. Sixteen $F_1$ sires and 64 $F_1$ dams were randomly chosen to produce a total of 640 $F_2$ offspring. In the reciprocal design, three $F_0$ grand sires of A breed and 15 $F_0$ grand dams of B breed were mated to generate 10 $F_1$ offspring per dam. Eight $F_1$ sires and 32 $F_1$ dams were randomly chosen to produce 10 $F_2$ offspring per $F_1$ dam, totaling 320 $F_2$ offspring. Another mating set comprised three $F_0$ grand sires of B breed and 15 $F_0$ grand dams of A breed to produce the same number of $F_1$ and $F_2$ offspring. A chromosome of 100 cM was simulated with large, medium or small QTL with fixed or different allele frequencies in parental breeds. A series of tests between Mendelian and POE models were applied to characterize QTL as Mendelian, paternal, maternal or partial expression QTL. The overall detection powers were similar between the two mating designs. However, the proportions of paternally expressed QTL that were declared as paternal QTL type were greater in the reciprocal cross design than in the one-way cross, and vice versa for Mendelian QTL. When QTL alleles were segregating in parental breeds, a significant proportion of Mendelian QTL were spuriously declared POE QTL, suggesting that care must be taken to characterize imprinting QTL in a QTL mapping population with a small number of $F_1$ parents.

• Asian-Australasian Journal of Animal Sciences
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• 제32권12호
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• pp.1816-1825
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• 2019

Objective: We tried to analyze allele-specific expression in the pig neocortex using bioinformatic analysis of high-throughput sequencing results from the parental genomes and offspring transcriptomes from reciprocal crosses between Korean Native and Landrace pigs. Methods: We carried out sequencing of parental genomes and offspring transcriptomes using next generation sequencing. We subsequently carried out genome scale identification of single nucleotide polymorphisms (SNPs) in two different ways using either individual genome mapping or joint genome mapping of the same breed parents that were used for the reciprocal crosses. Using parent-specific SNPs, allele-specifically expressed genes were analyzed. Results: Because of the low genome coverage (${\sim}4{\times}$) of the sequencing results, most SNPs were non-informative for parental lineage determination of the expressed alleles in the offspring and were thus excluded from our analysis. Consequently, 436 SNPs covering 336 genes were applicable to measure the imbalanced expression of paternal alleles in the offspring. By calculating the read ratios of parental alleles in the offspring, we identified seven genes showing allele-biased expression (p<0.05) including three previously reported and four newly identified genes in this study. Conclusion: The newly identified allele-specifically expressing genes in the neocortex of pigs should contribute to improving our knowledge on genomic imprinting in pigs. To our knowledge, this is the first study of allelic imbalance using high throughput analysis of both parental genomes and offspring transcriptomes of the reciprocal cross in outbred animals. Our study also showed the effect of the number of informative animals on the genome level investigation of allele-specific expression using RNA-seq analysis in livestock species.

• 한국융합학회논문지
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• 제12권9호
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• pp.229-238
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• 2021

본 연구의 목적은 상호동료 교수법을 적용한 핵심기본간호술 자율실습(투약단원을 중심으로)이 간호대학생의 학습에 미치는 영향을 심층적으로 이해하기 위함이다. 연구참여자는 간호학과 4학년 학생 15명이었으며, 심층면담을 통해 자료를 수집하였다. 수집된 자료를 분석한 결과, '학습동기 유발', '자기주도 학습', '성취도 향상', '학습요구 불충족'의 4개의 주제가 도출되었다. 상호동료 교수법을 적용한 핵심기본간호술 자율실습은 참여자들에게 편안한 환경과 자신감 향상으로 학습동기를 유발하고, 주도적인 사전학습 및 학습자의 언어로 학습할 기회를 얻는 긍정적인 경험이 있었다. 하지만, 추가적인 지식확장이 어렵고 충분하지 않은 학습요구로 불안감을 느끼는 경험도 있었다. 이에 상호동료 교수법을 자율실습에 적용할 때, 교수자는 학습자의 학습요구를 충분히 만족시키는 방안을 적용하면 효과적일 것으로 생각된다.

• 한국산학기술학회논문지
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• 제21권10호
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• pp.558-566
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• 2020

급변하고 융·복합화되는 비즈니스 환경 속에서 기업의 생존과 지속적 성장을 위해 신제품 개발은 필수적이며, 신제품 개발 성과 향상을 위해서 기업이 내·외부 파트너와 협업하는 것은 매우 중요하다. 본 연구에서는 신제품 개발(부서간 협업, 공급자 협업) 및 협업 커뮤니케이션(형식성, 상호피드백)이 협업 성과와의 통합적인 관계를 규명하는 것을 목적으로 하였다. 연구대상은 신제품 개발 경험이 있는 1년 이상의 제조업 종사자를 대상으로 실시하였다. 272부의 표본자료를 사용하여 신뢰성, 타당성 분석을 실시하였다. 연구결과를 요약하면 다음과 같다. 첫째, 신제품개발 협업의 요인인 부서간 협업과 공급자 협업은 각각 형식성에 통계적으로 정(+)의 영향을 미치는 것으로 나타났다. 둘째 형식성은 상호피드백에 통계적으로 정(+)의 영향을 미치는 것으로 나타났다. 셋째 형식성은 협업 성과에 정(+)의 영향을 미치는 것으로 나타났다. 넷째 상호피드백은 협업 성과에 정(+)의 영향을 미치는 것으로 나타났다. 따라서, 성공적인 신제품 개발을 위해 제조기업은 효율적인 경영전략을 세우고, 공급자간 상호 협업관계를 기반으로 기업의 생산성과 효율을 극대화할 수 있도록 공식적이고 상호 호혜적으로 의사소통하는 것이 중요하다. 이를 위해 기업은 내외부 파트너의 요구에 민첩하게 대응하기 위해 효과적인 의사소통 전략을 계획할 필요가 있음을 의미한다.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.475-479
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• 1998

Data on body weights were analyzed in the four genetic groups from all possible crosses of two subspecies of mice to estimate average direct genetic effects (ADGE), average maternal genetic effects (AMGE) and heterotic effect (HE). The genetic groups used were $CF_{{\sharp}1}$ laboratory mouse (Mus musculus domesticus), Yonakuni wild mouse (Yk, Mus musculus molossinus yonakuni) and two reciprocal $F_1$ crosses of them, CY and YC. First symbol in the reciprocal $F_1$ represent subspecies of dam. Body weight at 1 (Wk1), 3 (Wk3), 6 (Wk6) and 10 weeks of age (Wk10) were analyzed from 258 mice of the four genetic groups. The model used to evaluate body weights included main effects of genetic group and sex, and interaction effect between genetic group and sex. The ADGE and the AMGE were estimated as deviations of Yk from $CF_{{\sharp}1}$. The HE was estimated from the differences between the reciprocal $F_1$ and the midparent mean. Results of this study showed that all effects, except sex and interaction between genetic group and sex at Wk1 and Wk3, were highly significant source variation (p < 0.01). The ADGE were positive and highly significant (p < 0.01) at all ages studied for both sexes, while the AMGE were highly significant at Wk3, Wk6 and Wk10. The ADGE were larger in contributing effect on body weight differences than the AMGE. The positive value of the HE were observed at all ages for males, while for females the positive effects occured from birth through weaning.