• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.24.1-24.1
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• 2006

• Journal of the Korean Wood Science and Technology
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• 제39권5호
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• pp.437-443
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• 2011

In Korea, there has been a traditional post and beam wood construction with large roof load. Because a large friction is generated in wooden joint or members, it is important to evaluate the friction between wood members according to wood direction. Because most of studies have been concerned with friction between wood and steel, excluding effect of real area of contact, there are a few studies on the friction between wood members. The object of this study was to evaluate friction or coefficient of friction according to normal force and real area of contact of wood. With Japanese larch (Larix kaempferi) test specimens, five steps of normal force and combinations of test were prepared. Results indicated that normal force had almost no affection on the friction, however there was difference about friction or coefficient of friction according to real contact conditions of wood grain and contact area.

• 한국컴퓨터정보학회논문지
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• 제21권1호
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• pp.85-90
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• 2016

In this paper, the biometric data is transmitted in real time from the IoT environment is runoff, forgery, alteration, prevention of the factors that can be generated from a denial-of-service in advance, and the security strategy for the biometric data to protect the biometric data secure from security threats offer. The convenience of living in our surroundings to life with the development of ubiquitous computing and smart devices are available in real-time. And is also increasing interest in the IOT. IOT environment is giving the convenience of life. However, security threats to privacy also are exposed for 24 hours. This paper examines the security threats to biological data to be transmitted in real time from IOT environment. The technology for such security requirements and security technology according to the analysis of the threat. And with respect to the biometric data transmitted in real time on the IoT environment proposes a security strategy to ensure the stability against security threats and described with respect to its efficiency.

• Corrosion Science and Technology
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• 제8권6호
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• pp.243-250
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• 2009

When a steel sheet pipe applied to marine environment, an anti-corrosive coating should be treated to obtain long-term life-time for steels, especially, splash zone. Although anti-corrosive property of coatings is required to be tested in real marine environment, it is difficult because of long test time such as 20 years or more time. Therefore, we used cyclic corrosion tester in a laboratory, which has similar conditions with salt-dry-wet process such as real marine environment. Anti-corrosive properties of the coatings and two steels were tested their anti-corrosive properties under cyclic corrosion test conditions(KS D ISO 14993) and the results were compared with estimate life-time in real marine environment. According to cyclic corrosion test, accelerated corrosive factor of each anti-corrosive coating was investigated accelerated corrosive factor from impedance with EIS method. Accelerated corrosive factor of type SS400 carbon steel and A690 was also investigated their accelerated corrosive factor from the regression curves of weigh loss results. One of the anti-corrosive coatings showed about 50 years life-time compared with standard sample life-time. Carbon steel SS400 showed from 0.1 mm/yr to 0.06 mm/yr as its corrosion rate.

• 한국학교수학회논문집
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• 제10권1호
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• pp.45-69
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• 2007

21세기 지식 기반, 정보화 기반 사회는 수학을 단순히 적용하는 능력이 아닌 실생활이나 다른 교과 영역에서 수학적 지식을 사용하여 문제를 구성하고 해결하는 문제 해결력 등의 수학적 힘(Mathematical power)을 필요로 한다. 수학적 힘을 기르기 위해서는 수학의 기본 지식, 추론 능력, 문제 해결력, 수학적 아이디어의 표현 및 교환능력, 그리고 사고의 유연함, 인내, 흥미, 지적 호기심, 창의력을 길러 주는 다양한 교수 학습 방법이 필요하다. 본 연구에서는 다양한 학습 매체를 이용한 실생활 중심의 교수 학습 지도안을 개발하고 이를 통하여 학생들의 수업에 대한 반응과 수학에 대한 인식 변화를 분석하였다.

• 한국컴퓨터정보학회논문지
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• 제25권11호
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• pp.255-262
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• 2020

본 연구는 스마트폰 사용이 활성화된 사회 환경 속에서 발달장애 학생이 스마트폰 애플리케이션을 사회 실생활 도구로 효과적으로 활용할 수 있는 방안에 관해 '발달장애 학생, 정보사회, 스마트폰 사용, 애플리케이션 활용' 주제 관련 선행연구 문헌들을 검토하여 다음과 같이 살펴보았다. 첫째, 정보사회를 살아가는 발달장애 학생의 스마트폰을 활용한 사회통합 능력 향상 지원 방안을 기술한다. 둘째, 발달장애 학생의 사회 실생활을 지원해 줄 수 있는 스마트폰 애플리케이션 유형을 기술한다. 셋째, 발달장애 학생의 사회 실생활 적응을 위한 스마트폰 애플리케이션 요구 영역을 기술한다. 이 연구 결과는 발달장애 학생이 정보사회에서 스마트폰의 다양한 기능을 익히고 배워 이용함으로써 주변 사람들과 상호교류하여 의사소통능력을 향상시키고 직업재활과 사회통합에 도움이 되도록 적절한 애플리케이션을 개발하여 교육적으로 지원, 제공하는 기초자료로 활용될 수 있을 것이다.

• 한국포장학회지
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• 제17권2호
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• pp.33-37
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• 2011

변패성 식품의 하나로서 양념돼지불고기의 미생물적 저장수명을 실시간적으로 제어하는 방법으로서 포장 내 $CO_2$ 농도의 측정을 이용하는 방법을 고안하고 동적온도조건에서 그 적용가능성을 확인하였다. 식품으로부터의 $CO_2$ 생산 속도 혹은 포장 내 $CO_2$ 농도변화가 증가하기 시작하는 시점이 미생물적 한계품질에 해당되었고, 이를 저장수명의 결정의 지표로 사용할 수 있는 것으로 나타났다. 이는 또 특정 $CO_2$ 농도에 도달되는 시점, 즉 $CO_2$ 농도변화의 유도기와도 일치하였다. 제안된 logic은 $CO_2$ 농도를 실시간으로 측정하고 무선으로 데이터를 전송할 수 있는 sensor 시스템에 의하여 가능성이 확인되었다.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.280-289
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• 2021

Genetic markers currently used for the discrimination of Lactobacillus delbrueckii subspecies have low efficiency for identification at subspecies level. Therefore, our objective in this study was to select novel genetic markers for accurate identification and discrimination of six L. delbrueckii subspecies based on pangenome analysis. We evaluated L. delbrueckii genomes to avoid making incorrect conclusions in the process of selecting genetic markers due to mislabeled genomes. Genome analysis showed that two genomes of L. delbrueckii subspecies deposited at NCBI were misidentified. Based on these results, subspecies-specific genetic markers were selected by comparing the core and pangenomes. Genetic markers were confirmed to be specific for 59,196,562 genome sequences via in silico analysis. They were found in all strains of the same subspecies, but not in other subspecies or bacterial strains. These genetic markers also could be used to accurately identify genomes at the subspecies level for genomes known at the species level. A real-time PCR method for detecting three main subspecies (L. delbrueckii subsp. delbrueckii, lactis, and bulgaricus) was developed to cost-effectively identify them using genetic markers. Results showed 100% specificity for each subspecies. These genetic markers could differentiate each subspecies from 44 other lactic acid bacteria. This real-time PCR method was then applied to monitor 26 probiotics and dairy products. It was also used to identify 64 unknown strains isolated from raw milk samples and dairy products. Results confirmed that unknown isolates and subspecies contained in the product could be accurately identified using this real-time PCR method.

• Journal of Microbiology and Biotechnology
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• 제29권10호
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• pp.1543-1552
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• 2019

Salmonella is a common zoonotic and foodborne pathogen that causes high morbidity and mortality in developing countries. In this study, we established and validated a polymerase spiral reaction (PSR) assay which targeted the conserved invasion gene (invA) of Salmonella by SYBR Green I indicator methods. Subsequently, assays for determination of the optimal conditions for optimal specificity and sensitivity of PSR were performed. We performed comprehensive evaluations using loop-mediated isothermal amplification (LAMP) and real-time PCR. A total number of 532 samples of daily food were analyzed by PSR. Twenty-seven bacterial strains were tested in the specificity assay, from which positive results were obtained only for 14-Salmonella strains. However, none of the 13 non-Salmonella strains was amplified. Similarly with LAMP and real-time PCR, the detection limit of the PSR assay was 50 CFU/ml. The PSR method was also successfully applied to evaluate the contamination with Salmonella in 532 samples of daily food, corroborating traditional culture method data. The novel PSR method is simple, sensitive, and rapid and provides new insights into the prevention and detection of foodborne diseases.

• 한국패류학회지
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• 제27권4호
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• pp.387-393
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• 2011

The objective of this study was to develop a real-time PCR method for the rapid detection and quantification of the protozoan pathogen Perkinsus olseni using a TaqMan probe. For the standard, genomic DNA was extracted from $10^5$ in vitro-cultured P. olseni trophozoites, and then 10-fold serial dilutions to the level of a single cell were prepared. To test the reliability of the technique, triplicates of genomic DNA were extracted from $5{\times}10^4$ cells and 10-fold serial dilutions to the level of 5 cells were prepared. The standards and samples were analyzed in duplicate using an $Exicycler^{TM}$ 96 real-time quantitative thermal block. For quantification, the threshold cycle ($C_T$) values of samples were compared with those obtained from standard dilutions. There was a strong linear relationship between the $C_T$ value and the log concentration of cells in the standard ($r^2$ = 0.996). Detection of DNA at a concentration as low as the equivalent of a single cell showed that the assay was sensitive enough to detect a single cell of P. olseni. The estimated number of P. olseni cells was similar to the original cell concentrations, indicating the reliability of P. olseni quantification by real-time PCR. Accordingly, the designed primers and probe may be used for the rapid detection and quantification of P. olseni from clam tissue, environmental water, and sediment samples.