• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.107-112
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• 2004

The combining abilities in the 5 newly evolved thermo tolerant breeds viz., SR6, SR7, SR8 SR9 and SR10 of silkworm Bombyx mori L. and their 15 hybrids were made in a line${\times}$tester crossing programme. Data were analysed for seven quantitative traits i.e., pupation rate, cocoon yield, cocoon weight, cocoon shell weight, cocoon shell ratio, filament length and raw silk percentage under optimum room temperature $(25{\pm}{1^{\circ}C})$ conditions (In case of high temperature $(36{\pm}{1^{\circ}C})$ stress conditions five economic traits except filament length and raw silk percentage) with 3 widely adapted testers i.e., KA, CSR2 and CC1 as lines (females) and testers (males) respectively. The performance at high temperature and low humidity conditions aye only taken into consideration for selecting the best lines/hybrids. Among the lines SR6 exhibited positive General combining ability (GCA) effects for pupation rate, cocoon yield, cocoon weight and cocoon shell ratio traits, followed by SR7 for pupation rate, cocoon yield and cocoon shell weight and cocoon shell ratio. Among testers, KA exhibited positive GCA effects for two quantitative traits cocoon yield, cocoon weight and CSR2 for cocoon shell weight and cocoon shell ratio under adverse temperature conditions. The hybrid SR6${\times}$CC1 and SR7${\times}$CSR2 exhibited significant positive Specific combining ability (SCA) effects for majority of the traits in high temperature stress conditions of rearing. The better parent value of heterosis(Heterobeltiosis) was exhibited by the hybrid SR6${\times}$CC1 for pupation rate, cocoon yield, cocoon weight and cocoon shell weight and SR7${\times}$CSR2 for all the trails evaluated under high temperature conditions. Based on the results, the lines SR6 and SR7 was judged as best combiners and the hybrids SR6${\times}$CC1 and SR7${\times}$CSR2 can be selected for commercial exploitation in tropical climate.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.171-178
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• 2003

Four thermo-tolerant lines of silkworm, Bombyx mori, (L.) viz., A HT, B HT (Chinese type) and F HT, G HT (Japanese type) were evolved by utilizing the breeding resource material (identified from initial screening at a temperature of 31 ${\pm} 1^{\circ}C$ and relative humidity 85 ${\pm}$ 5%) through conventional breeding. These tolerant lines were crossed with productive breeds and forty four hybrids were evaluated on eight economic traits by the Multiple Trait Evaluation Index Method. Ten hybrids were short-listed based on the average evaluation index value larger than 50 for eight economic traits studied. The identified ten hybrids recorded higher index values (＞ 50) for most of the traits studied. Single hybrid G ${\times}$ CSR12 indicated average index value larger than 50 for six traits viz., pupation number (58), cocoon weight (67), shell weight (65), average filament length (74), raw silk % (69), reelability % (51) except for shell ratio % (41). The standard deviation of the cocoons in the above hybrid was 8.41 in the hybrid cocoon length and width measurement. However, two selected hybrids viz., A ${\times}$ CSR5 and G ${\times}$ CSR13 recorded average index value larger than 50 for all the traits viz., pupation number (57, 60), cocoon weight (50, 54), shell weight (56, 57), shell ratio percentage (59, 53), average filament length (55, 60), raw silk percentage (63, 67) and reelability percentage (53, 53). The standard deviation of the cocoons in the two selected hybrids viz., A ${\times}$ CSR5 and G ${\times}$ CSR13 was 8.41 and 8.06 respectively in the cocoon length and width measurement.

• 한국잠사곤충학회지
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• 제16권2호
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• pp.133-139
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• 1974

본실험은 상족기에 집중적으로 소요되는 노동력을 절감하기 위하여 숙잠의 자연등족을 촉진시키는 보조수단의 1종인 기피제에 대해서 연구하였는바 다음과 같은 결과를 얻었다. 1. 기피효과가 가장 현저한 것은 참나무계통이며 약쑥 개여뀌의 순으로 나타났다. 그 이외의 처리구도 기성등족제 シ ル ク ア ツ プ 처리구보다 등광률이 높은 것으로 미루어 보아 실용화가 기대된다. 2. 기선제의 조제방법은 증류액, 탕액 및 즙액의 형태로 조제하여 조강에 흡수시켜 시용하는 것이 등족효과가 우수하였다. 이 중에서도 가장 손쉬운 방법은 탕액이 라고 생각된다. 3. 기피제처리가 누에 생리에는 거의 영향이 없는듯하며 견질과 사질에도 별로 영향을 미치지 않는 것으로 보인다. 4. 본기피제를 잠좌에 처리했을 때 미숙잠도 등족하는 경향이 있으므로 기피제를 이용할 때는 숙화정도를 재일하게 해야만 더욱 효과적일 것으로 살펴진다.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.59-63
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• 2003

In the present study, hybrid vigour in terms of heterosis and over dominance of newly evolved bivoltine hybrids and their reciprocals raised from the promising newly evolved bivoltine breeds were calculated for the important parameters like pupation, cocoon yield, cocoon weight, shell weight, shell ratio, raw silk percentage, filament length and neatness. Through there was no significant difference among the hybrids for the characters pupation, cocoon yield and cocoon weight, the magnitude of heterosis and over dominance were varied significantly for these characters. However, significant differences were observed between new hybrids and control hybrid for the characters like cocoon shell weight, cocoon shell ratio, filament length and raw silk percentage. The results indicate that majority of new hybrids have shown higher heterosis and over dominance than control hybrid. The data also indicate that highest mean heterosis of all new hybrids was recorded for cocoon yield followed by filament length. Whereas highest mean over dominance was recorded for cocoon yield followed by cocoon weight. Negative mean heterosis and over dominance were recorded for the trait cocoon shell ratio. Based on the results, the importance of three hybrids viz., CSR2 ${\times}$ CSR4, CSR2 ${\times}$ CSR5 and CSR3 ${\times}$ CSR6 and their reciprocals (CSR4 ${\times}$ CSR2, CSR5 ${\times}$ CSR2 and CSR6 ${\times}$ CSR3) which exhibited significant heterosis and over dominance, for commercial exploitation was discussed.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.15-20
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• 2003

The present investigation was carried out to study the possible cause for reciprocal difference in silkworm hybrids. By utilising the polyvoltine race Pure Mysore (PM) and newly evolved breeds (CSR2, CSR5, CSR16 and CSR17), the direct and reciprocal crosses of polyvoltine ${\times}$ bivoltine and also bivoltine hybrids were studied. The hybrids of polyvoltine ${\times}$ bivoltine (direct) are superior to their reciprocal crosses in respect of cocoon yield, cocoon weight and filament length. The reciprocal crosses of polyvoltine ${\times}$ bivoltine are superior to their direct crosses in respect of fecundity and short larval duration. No significant differences were observed in the characters like cocoon shell ratio, raw silk percentage, denier, reelability and neatness in both polyvoltine ${\times}$ bivoltine direct crosses and their reciprocals. The expression of cocoon characters as a function of sex revealed that direct crosses (polyvoltine ${\times}$ bivoltine) showed higher cocoon weight, pupal weight, shell weight and longer filament length in females than the reciprocal crosses (bivoltine ${\times}$ polyvoltine), where as these characters in males were almost the same in both direct and reciprocal crosses, indicating that the sex-linked genetic factor played a more important role. it was clear that difference in cocoon yield observed in reciprocal crosses of polyvoltine ${\times}$ bivoltine was due to the low cocoon and shell weight in females which was turn due to presence of early maturity genes (Lme) linked with sex-chromosome (X) which effect on larvae period of the silkworm. In bivoltine hybrids, i.e., both direct and their reciprocals crosses, all the characters viz., hatching percentage, larval duration, survival, cocoon weight, cocoon shell weight, cocoon shell ratio, raw silk percentage, filament length, denier, reelability and neatness did not show any significant difference (except number of eggs laid by moth) which could account for presence of same maturity genes (Lm) in both direct and reciprocal crosses. it was clear that reciprocal differences occur when the hybrids are prepared from the parental strains with different voltinism.

• 한국잠사곤충학회지
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• 제10권
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• pp.81-88
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• 1969

견검정용 자견기 증자부에 Infra-red-ray Lamp를 설치한 후 1968년도 춘추잠견에 대하여 자견을 행하였다. 동시에 이들 원료견의 조사를 하여 해서상태와 생사량 및 부견사량 등 몇가지 항목에 대한 조사결과 다음과 같은 결론을 얻어 이에 보고하는 바이다. (1) IRR-Heater의 조사는 원료견의 해서를 향상시켰는데 원료견에 따라서 그 효과에 차이가 있어 춘잠견에서는 5∼9％, 추잠기에서는 2∼10％의 향상을 보았다. (2) 생사량비율에 있어서는 유의차는 있지 않으나 춘잠원료견에 따라서는 0.3∼0.6％, 추잠견에서는 0.2∼1.3％(지수)의 증수였다. (3) IRR-Heater의 조사본수별 효과도 원료견성상에 따라 불량견에서 2kW, 우량견에서는 1kW로서 그의 효과가 있었다. (4) 조사능률은 춘추잠견별로 최저 3％, 최고 14％(지수)의 행상을 보았다. 이상 해서율의 향상과 이에 따른 사조고장의 감소 및 능률의 향상과 생사량비율의 증수를 고려하여보면 현재 우리나라의 조사기가 자동화하여 (현 39.5％)가는 현실로 보아 전국 각제사공장의 기업제사를 더욱 합리적으로 경영키 위해서는 적외선처리로서 원료견의 해서를 도모해야할 것이다.

• International Journal of Industrial Entomology and Biomaterials
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• 제12권1호
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• pp.29-34
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• 2006

A breeding programme was initiated during 2001 utilizing two polyvoltine silkworm breeds viz. $BL_{69}$, an evolved breed tolerant to high temperature and MAR, comparatively resistant to Bombyx mori nuclear polyhedrosis virus (BmNPV) with the objective to develop robust polyvoltine breeds and hybrids. The breed $NP_1$ was developed by exposing the fifth instar larvae to high temperature $(36{\pm}1^{\circ}C)$, high Relative Humidity ($85{\pm}5%$ R.H.) and inoculating third instar larvae with BmNPV inoculum. At $F_{12}$, the breed was tested for hybrid forming ability utilizing six bivoltine silkworm breeds viz. $CSR_2,\;CSR_4,\;CSR_{17},\;CSR_{18},\;CSR_{19}\;and\;NB_4D_2$. The hybrid $'NP_1{\times}CSR_{17}'$ exhibited its superiority by recording 97.2% survival, 1.892 g cocoon weight, 0.406 g cocoon shell weight, 21.5% cocoon shell ratio, 16.6% raw silk percentage and 890 m filament length whereas the control $(PM{\times}CSR_2)$ has recorded 90.2% survival, 1.599 g cocoon weight, 0.304 g cocoon shell weight, 18.9% cocoon shell ratio, 13.1 % raw silk percentage and 768 m filament length. Commercial exploitation of the new $polyvoltine{\times}bivoltine$ hybrid in sericulture industry has been discussed.

• 한국잠사곤충학회지
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• 제15권1호
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• pp.37-48
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• 1973

The purpose of this studies is to improve the cohesion of raw silk through various analyses on cocoon drying, cooking, reeling, re-reeling, and on the properties of water. Also we investigated the correlation between silk testing items which we have reached to the following results. 1. Drying of cocoon When cocoons were slowly dried with 100$^{\circ}C$, the results of cohesion became much better. On the other hand, the results were considerably decreased in case the temperature with 115$^{\circ}C$. 2. Cooking of cocoon In case of the cooking of cocoon, we found that the result of cohesion was best with incomplete cooking, that of the control was next, while in over cooking, the results were very low. Also the results of cohesion were much better when using the method of over cooking with sericin arrestive agent than that of incomplete cooking with sericin agglutinating agent. 3. Reeling of cocoon A) When the temperature of reeling bath was 25-45$^{\circ}C$, the results of cohesion test were much better, but at the temperature below 25$^{\circ}C$ or above 45$^{\circ}C$, the results became worse. B) With out the process of croissieur, the results of cohesion were too bad, but in case of croissieur more than 1cm, cohesion became better rapidly. Further more, we understood that the results of cohesion were improving slightly with longer length of croissieur. C) When the velocity of reeling was increased, the results of cohesion also improved. The best results were shown when reeling velocity was 180-220 r.p.m. But when the velocity was increased more than 220 r.p.m., the results of cohesion got worse more or less. D) When the temperature of the drying pipe in reeling machine was raised, the results of cohesion also showed a tendency to improve. 4. Re-reeling A) We could net reach a conclusion as to have correlation between the number of dipping repeat in vacuum tank and the results of cohesion before re-reeling process. B) When we used Seracol 500 as an agglutination protective agent with l/1000 to l/2000 of water, the results of cohesion test were better. C) When we used Pearl-lite as an agglutination protective agent with 1/1000 to 1/2000 of water, the results of cohesion were considerably better. D) We gained tile best results when used Cohesion Improving Chemical, A-80, with 500-1500 times diluted. 1) Results of cohesion was improved when humidity was low or temperature was high in the rereeling machine. 5. Filature water A) The water pH near the isoelectric point of protein showed the best cohesion, but the farther water pH, the worser results were obtained. B) With the increasing of M-alkalinity in filature water, the results of cohesion were worse. Above all, we understood the tendency of the results of cohesion get worse when the M-alkalinity is increased above 200 ppm. C) By increasing the total hardness of the filature water, it improved the results of cohesion. Especially, when the total hardness was above 300ppm, the results were extremely high. 6. Effects combination of each results A) The result of effects combination in filature processes with the obtained best conditions was distinctively improved. But the results could not reach in mathematicaly double effect. When reelied under worse conditions, the results of cohesion test were too bad. There was "effect limit" for the promotion. B) Generally the results of cohesion were bad when the filature conditions(the temperature, pressure and the properties of water, etc) are processed as sericin loss to be high. On the other hand, the results were very good when lower sericin loss was controlled in filature conditions. C) When filature conditions such as reeling velocity and croissieur length provide pysical cohesion ability and when raw silk dry fast during reeling and re-reeling, we found the result of cohesion was better. 7. Correlation of silk testing items. A) A negative correlation exists between the results of cohesion test and cleanness defect. Another word, the result of cohesion test was found to be worse as cleanness defect increased. B) In cleanness, cohesion has negative correlation against the number of slugs, but we could not find any correlation against long loops, loose ends. C) Cohesion has negative correlation against average neatness and low neatness defect. The better the results of neatness respectively, the better the results of cohesion found. D) There is no correlation between tenacity and the results of cohesion test, but there was high positive correlation between the results of elongation and those of cohesion test. The more elongation, the better the results of cohesion was found.

• International Journal of Industrial Entomology and Biomaterials
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• 제1권2호
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• pp.155-159
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• 2000

In the spring silkworm rearing period of year 1998, some nutritional aspects were studied in the Agricultural Research Station of the Komotini town in Greece, to investigate the feeding behaviour of some Bulgarian silkworm hybrids, suitable for spring rearing, during the fourth and fifth instal under the local climatic conditions. The silkworms were fed by locally cultivated Japanese Kinriu mulberry (Morus alba L.) variety leaves. Eight Bulgarian hybrids had been used, namely Vratza-53xVratza-52, Ukraine-20xVratza-53, Super 1xHessa 2, Merefa 2xVratza 35, as well as their reciprocal crosses. The studies showed out a remarkably higher feed intake and feed utilization by the hybrids Hessa 2xSuper-1, Merefa 2xVratza 35 and Vratza-52xVratza-53. The same hybrids showed an efficient food utilization by means of daily growths cocoon shell ratio and raw silk output. In terms of food to silk conversion efficiency Hessa 2xSuper-1 hybrid gave the best results. Larval stage duration for fourth and fifth instar was not affected by feed intake and utilization. All hybrids showed a good adaptation to the local environment and their feeding performance was equal to the international existing standards. All calculated parameters were found to be around the mean values of other up to date presented results and thus considered as acceptable for the needs of the local production.

• International Journal of Industrial Entomology and Biomaterials
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• 제11권2호
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• pp.119-124
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• 2005

To determine the level of heterosis, higher cocoon shell weight multivoltine congenic lines (Con. L) and bivoltine syngenic lines (Syn. L) of silkworm were used for crosses. First filial generations $(F_1s)$ expressed heterobeltiotic genetic interaction at significant magnitude (p < 0.01) for single cocoon shell weight (SCSW). The other linked characters viz., single cocoon weight (SCW) and yield by weight per 10, 000 larvae were also significantly higher (p < 0.01) than the better parental lines. All the hybrids showed significant improvement for these aforesaid characters over standard heterosis (Standard check). The reeling parameters viz., filament length, raw silk, neatness, cohesionstrokes etc, also showed improvement among the hybrids than check in congenial environment. Overall results suggested that the cross between congenic and syngenic lines provide better heterosis with good quality silk than conventional hybrids and may be used for commercial exploitation.