• 동의생리병리학회지
• /
• 제19권5호
• /
• pp.1330-1336
• /
• 2005

This study was carried out to investigate the motor activity and glucose transport and metabolism of Gaewool-Whadam-Jian(GWJ) in rat gastro-intestinal tract. The motor activity of the rat gastro-intestinal tract has been investigated by means of measuring barium sulfate passage degrees. Atropine treatment significantly delayed barium sulfate transit, and GWJ pretreatment increased intestinal motor activity, but not significant. GWJ administration showed no toxicity to kidney and liver. Transport and metabolism of glucose were studied in everted sac of rat small intestine with incubation under several conditions. The transport and metabolism of glucose were greater at jejunum than ileum. So, everted jejunum of rat were used to study the effect of GWJ. When GWJ were treated, the concentration of glucose were higher than untreated group. This result was thought to be influenced by the glucose in GWJ. When 2, 4 dinitrophenol and phlorizin were treated, the transport and metabolism of glucose were decreased, but GWJ treated together, the concentration of glucose in serosal solution increased. Gastric juice secretion and total acidity significantly decreased by administration of GWJ through duodenum region. The mechanism of effect of GWJ was still unidentified, Dut through continuous investigation, the effect of GWJ should be investigated.

• 고려인삼학회:학술대회논문집
• /
• 고려인삼학회 2002년도 학술대회지
• /
• pp.277-287
• /
• 2002

The chemopreventive effects of ginseng on rat carcinogenesis models were investigated, In the present study, the inhibitory effects of white and red ginseng on tumor development were examined using medium-term liver, initiation and medium-term multi-organ carcinogenicity bioassay systems. No modifying potential of the ginsengs was evident in terms of the numbers or areas of glutathione S-transferase placental form (GST -P)-positive foci, which is a marker of preneoplastic lesion in rat livers. However, white ginseng, but not red ginseng was found to decrease the incidences of adenocarcinoma of the small intestine and colon in the medium-term multi-organ carcinogenesis model. These results indicate that white ginseng may have inhibitory effects on progression stage of rat intestinal carcinogenesis, but the influence is not strong. Ginseng is unlikely to have promoting or inhibitory effects in other organs under the present type of experimental conditions. Possible application on ginseng for chemoprevention of colon cancer in humans, can be concluded given the lack of obvious adverse effects.

• 한국독성학회:학술대회논문집
• /
• 한국독성학회 2001년도 International Symposium on Effects of Edible Phytochemicals and Their Synthetic Derivatives on Carcinogenesis and Mutagenesis
• /
• pp.15-15
• /
• 2001

In the present study, we examined the chemopreventive effects of indole-3-carbinol (I3C), a constituent of cruciferous vegetables (the Family of Cruciferae) such as cabbages, cauliflowers and broccoli on multiple intestinal neoplasia (Min) genetic mouse model and on mouse colon carcinogenesis induced by azoxymethane (AOM) as well as on rat mammary carcinogenesis induced by 7, 12-dimethybenz［$\alpha$］anthracene (DMBA).(omitted)

• 한국독성학회:학술대회논문집
• /
• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
• /
• pp.58-58
• /
• 2001

In the present study, we examined the chemopreventive effects of indole-3-carbinol (I3C), a constituent of cruciferous vegetables (the Family of Cruciferae) such as cabbages, cauliflowers and broccoli on multiple intestinal neoplasia (Min) genetic mouse model and on mouse colon carcinogenesis induced by azoxymethane (AOM) as well as on rat mammary carcinogenesis induced by 7, 12-dimethybenz［$\alpha$］anthracene (DMBA).(omitted)

• 동의생리병리학회지
• /
• 제20권4호
• /
• pp.924-928
• /
• 2006

This study was undertaken to determine the effects of Zusanli(ST 36) electro-acupuncture(EA) on the small intestinal motility in rat. The motor activity of the rat small intestine was evaluated by intestinal transfortation rate. Changes in the motility in vivo of ileum was measured before and 10 minutes after acupuncture. In order to examine whether EA effect was affected by stimulated duration and left or right sides of ST36 acupoint, both of the difference effects of left or right sides on acupoints of ST 36 and the stimulated duration of 10, 20, 30 min. were investigated on the motility in vivo of ileum. These results suggest that effect of Zusanli(ST36) for increasing the small intestinal motility should stimulate more than 20 minutes, left ST36 EA has stronger effect than right ST36 EA.

• 동의생리병리학회지
• /
• 제25권6호
• /
• pp.975-981
• /
• 2011

The aim of this study was to observe the effect of moxibustion at matched acupoints of ST36, ST37 and ST39 in rats with sex and age. This study measured small intestinal motility in rats. First, cauterize with moxa was applied to the ST36, ST37 and ST39 in rats classified by sex and age under enflurane anesthesia. And then same treatment is done to matched acupoints of ST36, ST37 and ST39. In each groups of ST36, ST37 and ST39, the small intestinal motility was increased in 5, 6, 8 weeks male and 5 weeks female rats at ST36, 6, 7 weeks male and 5, 6, 8 weeks female rats at ST37, 5, 6 weeks male and 5 weeks female rats at ST39. In matched acupoints, the small intestinal motility was increased in 5, 8 weeks male and 5, 6, 7, 8 weeks female rat at ST36+ST37, 5 weeks male and 6, 7, 8 weeks female rats at ST36+ST39, 5, 7 weeks male and 5, 8 weeks female rats at ST37+ST39. The effects of moxibustion at each acupoints decreased by advancing age and when these acupoints were combined, the effective results were shown in female rats of all age group. These results suggest that when each acupoints and matched acupoints was chosen in moxibustion treatment, the sex and age of individual is worth consideration.

• 대한의생명과학회지
• /
• 제25권4호
• /
• pp.339-347
• /
• 2019

Saururus chinensis (SC) belongs to the dicotyledonous plants, and its roots, leaves and flowers are white, so it is named three hundred and seconds. It is mainly distributed in Korea, China and Japan. In Korea, it is a native plant distributed in Jejudo and Jirisan areas. It has been known to improve blood circulation, anticarcinogenic effects and purge. However, studies of the efficacy on digestive system is few. This study was conducted to evaluate the effects of oral administered-SC extract in loperimide-induced constipation rats. The amount, weight and water content of the stools were measured. The number and type of stools in the large intestines were measured, and the amount of intestinal mucus was analyzed by serological analysis and histologic special staining. The severity of constipation in SC groups was significantly less than that in control group (non-SC rats). Digital Image processing also showed weaker inflammation on the large intestines of SC groups than that of control group (non-SC group). Especially, with increased dose dependent manner of SC extract, the amount and integrity of intestinal mucus increased. These results suggest that SC extract may prevent the symptoms of constipation.

• 동의생리병리학회지
• /
• 제21권3호
• /
• pp.748-754
• /
• 2007

Electroacupuncture is the combined treatment of mechanical and electric stimuli on the needle head, on which electricity runs after acupuncturing at the acupuncture points. It is reported that its treatment effect depends on the parameter of stimulus, which is wave of stimulus, frequency, voltage, and duration of electric current. To get the effective treatment, stimulus intensity over threshold is needed. We planned to experiment whether acupuncture effect is affected by sex, age, and weight. After we conducted electric stimulus at Zusanli(ST 36) which is known to increase small intestinal motility in normal rats, we observed the effects and got the following results. Though the increased effects of small intestinal motility of electro-acupuncture at Zusanli(ST 36) did not show the difference of sex, this effect was observed to have a tendency to decrease according to weight increase without distinction of sex. But the effect of electro-acupuncture shown in 4, 5, 7 weeks old without distinction of sex was observed to have a tendency to decrease with the growing of weeks-old in the male rat group. And the significant change without specific tendency was observed in the 4, 5 weeks old female rat group. This result suggested that sex, weight, and age should be considered to get the efficient electro-acupuncture. The more experimental study should be advanced to investigate the correlation between the factors that can affect the acupuncture treatment, and, afterward, the interconnection into the clinical use.

• Neonatal Medicine
• /
• 제17권1호
• /
• pp.44-52
• /
• 2010

목 적 : 경구 내독소와 저산소 자극을 이용하여 신생쥐에서 실험적 괴사성 장염(necrotizing enterocolitis, NEC)을 유발하고 그 병태생리에서 세포자멸사의 역할을 알아본다. 방 법:신생쥐에 경구로 내독소(5 mg/kg)를 투여하고 8% 저산소 자극을 주어 NEC를 유발하고 성공적으로 NEC가 유발된 군에 caspase 억제제를 전처치하고 비교하였다. 장 조직의 병리학적 분석은 hematoxylin-eosin 염색한 슬라이드로 하였고 세포자멸사는 TUNEL 염색과 장 조직 caspase-3 활성으로 분석하였다. IEC-6세포주에 내독소와 저산소를 처리한 후 세포자멸사와 Bax, Bcl-2, Fas, FasL의 발현을 분석하였다. 결 과:경구 내독소(5 mg/kg)와 반복적으로 60분간 2회 투여된 8% 저산소 자극에 의해서 NEC와 유사한 장병변이 신생쥐에서 유발되었다. 장 조직은 세포자멸사와 caspase-3 활성의 증가를 보여주었다. Caspase 억제제 전처치에 의해서 세포자멸사와 NEC의 중증도가 모두 감소하였다. IEC-6 세포주도 내독소와 저산소 자극에 의해서 세포자멸사와 caspase-3 활성의 증가를 보여주었으며 Bax/Bcl-2 ratio가 유의하게 증가하였다. 결 론:경구 내독소와 저산소를 이용한 본 NEC 신생쥐 모델은 caspase-3 활성에 의해 매개되는 장 상피세포의 세포자멸사가 병태생리에 관여함을 보였다. 본 동물모델은 임상에서 흔히 접할 수 있는 자극을 적용하였기 때문에 그 병태생리와 치료적 시도의 연구에 더욱 유용하리라고 본다.

• 동의생리병리학회지
• /
• 제21권6호
• /
• pp.1549-1554
• /
• 2007

To verify the effects of JAUN-1, which is a water-extracted herbal mixture, on gastroenteric disorders induced by 0.1 percent of iodoacetamide (IA) in rats. We divided four groups, $Na{\"{\i}}ve$ + Distilled Water (DW), 0.1% IA + DW, 0.1% IA + Proton pump inhibitor (Lansoprazole, 5 mg/kg) and 0.1% IA + Herbal mixture (JAUN-1, 50mg/kg) and performed following experimental methods to confirm its advantageous effects against ulcerogenic stomach in rats induced by 0.1% IA; cell cytotoxicity, analysis of lesions score, Hematoxylin & Eosin (H&E) stain, RT-PCR for ${\beta}-actin$, COX-1 and COX-2 and evaluation of intestinal prokinetic activity. No cytotoxicity was elucidated at the concentration of 1, 5, 10, 50, 100, $500\;{\mu}g/ml$ and 1mg/ml JAUN-1 through MTT Assay using by human stomach epithelial AGS cells, respectively. In addition, the JAUN-1 treated group and the lansoprazole treated group significantly decreased in lesions score compared to the DW treated group in the gastritis induced rat model, and results of immunohistochemistry by H&E staining showed that histological recovery in Proton Pump Inhibitor (PPI) and JAUN-1 treated groups rather than the DW administrated group. Another outcome was that ${\beta}-actin$ relative COX-2 expression level was significantly promoted in the DW treated group while ${\beta}-actin$ relative COX-1 expression level was no meaningful change in this rat model. Finally, intestinal prokinetic activity was recovered from low level of prokinetic activity due to 0.1% IA induced gastritis to the similar level of Normal group. These results suggested that JAUN-1 may have beneficial effects against 0.1% IA-induced gastritis rat model through decreasing lesions score, histological recovery, ${\beta}-actin$ relative COX-1, 2 expression level and prokinetic activity.