• 한국멀티미디어학회논문지
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• 제16권8호
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• pp.914-926
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• 2013

최근 보안 시스템 중에서 지문인식을 이용한 보안 시스템은 다른 보안 시스템에 비해 유일성과 편의성 등의 장점을 가짐으로써 많은 사람들이 관심을 갖는 분야이다. 지문인식 시스템에 있어서 가장 중요한 사항은 실제 지문과 영상을 통해 얻어진 지문간의 정합에서의 정확성과 지문 인식을 위해 사용하는 영상처리 알고리즘들을 신속하게 처리하는 데 있다. 기존의 지문인식 시스템은 특징 추출을 위해 사용하는 알고리즘들의 처리 시간을 줄이기 위해 전체 처리과정 중 일부 과정들을 생략함으로써 처리시간을 단축한다. 하지만 이 방식은 처리시간을 단축시킬 수 있는 반면 특징 추출에 대한 정확도가 떨어진다. 따라서 본 논문에서는 특징 추출에 대한 정확도를 높이기 위해 전체 처리 과정을 사용하면서 동시에 처리시간도 단축시킬 수 있는 다중 접근 메모리 시스템을 이용한 지문인식 특징 추출 알고리즘을 구현하였고, 구현된 시스템을 사용하였을때 다중접근 메모리 시스템과 시리얼 프로세서의 결과에 대해 correlation을 이용한 검증을 통해 제안된 방법의 신뢰도를 확인하였으며, 시리얼 프로세서에 비해 MAMS-PP64를 이용한 방법은 수행시간에서 약 1.56배 향상되었음을 확인하였다.

• 센서학회지
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• 제27권4호
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• pp.237-241
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• 2018

The real-time enrichment and detection of pathogens are serious issues and rapidly evolving field of research because of the ability of these pathogens to cause infectious diseases. In general, bacterial detection is accomplished by conventional colony counting or by polymerase chain reaction (PCR) after DNA extraction. As colony counting requires considerable time to cultivate, PCR is an attractive method for rapid detection. A small number of pathogens can cause diseases. Hence, a pretreatment process, such as enrichment is essential for detecting bacteria in an actual environment. Thus, in this study, we developed a microfluidic chip capable of performing rapid enrichment of bacteria and the extraction of their genes. A lectin, i.e., Concanavalin A (ConA), which shows binding affinity to the surface of most bacteria, was coated on the surface of magnetic particles to nonspecifically capture bacteria. It was subsequently concentrated through magnetic forces in a microfluidic channel. To lyse the captured bacteria, magnetic particles were irradiated by a wavelength of 532nm. The photo-thermal effect on the particles was sufficient for extracting DNA, which was consequently utilized for the identification of bacteria. Our device will help monitor the existence of bacteria in various environmental situations such as water, air, and soil.

• 한국연초학회지
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• 제14권2호
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• pp.159-167
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• 1992

It would be clear that the constituents of the leaf surface lipid is ye비 important as an evaluation index of tobacco leaf quality since the quality of tobacco specific aroma with leaf species depends on the contents of the lipid and the strength of the aroma is determined by the amounts of the lipid secreted. For the reason, a rapid and peproducible method to quantify DVT, which is a kind of lipid, has been studied. The biosynthesis procedure of DVT in leaf growing processes, and the volatile or decompositional characters of DVT in leaf drying processes were also discussed. In consequence, it might be possible to get the data available to the cultivation of better tobacco leaf and the manufacture of cigarettes with better aroma and taste. The results obtained from this study are as follows. 1. Chloroform/dichloromethane solvent was better than chloroform alone for DVT extraction. The extraction yields of the leaf surface lipid were about 5% 2. The extractives with dichloromethane were treated by silylation with BSTPa and the quantitative analysis of DVT was carried out using SE -54 fused silica capillary column. It was found that rapid and reproducible data could be obtained from these methods. 3. In flue - cured tobacco species, DVT contents were $30.3\mu\textrm{g}/cm^2$ in the beginning stage of leaf drying processes and $12.1\mu\textrm{g}/cm^2$ corresponded to 30% levels of the beginning stage, in the end stage. 4. DVT contents in Burley mere 2 times as large as those in fluecured tobacco. DVT in the upper stalk position of leaf was 3 times larger than that in the lower stalk position. 5. DVT of tobacco leaves was decomposed by $SO_2$ gas or the sun light. The decomposition rate was largest in the sample used methanol as a extraction solvent.

• 대한원격탐사학회지
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• 제36권5_1호
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• pp.823-833
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• 2020

안정적인 작물 생산을 위하여 국내 농업지역에 대한 효과적인 작황 모니터링 기법의 요구가 증대되고 있다. 본 연구에서는 작물 재배지역 추출을 위하여 딥러닝 기법을 이용한 분류 모델을 개발하고, 이를 위성영상에 적용하고자 하였다. 이를 위하여, 식생분석에 유용한 blue, green, red, red-edge, NIR 밴드를 포함하고 있는 RapidEye 위성영상을 이용하여 작물 재배지역에 대한 훈련자료를 구축하고, 이를 활용하여 국내 양파 및 마늘 작물에 대한 재배면적을 추정하고자 하였다. 대기보정된 RapidEye 위성영상을 활용하여 훈련자료를 구축하였으며, 작물지역의 분류를 위하여 대표적인 의미론적 분할을 위한 딥러닝 모델인 FC-DenseNet을 이용하여 딥러닝 모델을 생성하였다. 최종적인 작물 재배지역은 지적도와의 결합을 통하여 객체 기반의 자료로 생성하였다. 실험결과, 대기보정된 훈련자료를 이용하여 학습된 FC-DenseNet 모델은 훈련에 사용되지 않은 타 지역의 작물 재배지역을 효과적으로 검출할 수 있음을 확인하였다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.401.3-402
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• 2002

Enalapril (ENP) maleate is effective drug for the treatment of renivascular hypertension and heart failure. ENP acts as inhibitor of the enzyme angiotensin-convertase (ACE-inhibitor) and metabolized to enalaprilat (ENPT), which is the active metabolite that is really responsible for the therapeutic action. In the present study, a sensitive and rapid liquid chromatography/ electrospray ion trap tandem mass spectrometry (LC/MS/MS) method combined with high-throughput solid phase extraction (SPE) has been developed and validated for the simultaneous quantitative determination of ENP and ENPT in human plasma. (omitted)

• Food Science and Biotechnology
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• 제18권2호
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• pp.407-412
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• 2009

In this study, a rapid and efficient concentrating procedure that can be used for detecting viruses in vegetables was developed. The Sabin strain of poliovirus type 1 was used to evaluate the efficiency of virus recovery. The procedure included: (a) elution with 0.25 M threonine-0.3 M NaCl pH 9.5; (b) polyethylene glycol (PEG) 8000 precipitation; (c) chloroform extraction; (d) 2$^{nd}$ PEG precipitation; (f) RNA extraction; (g) reverse transcription-polymerase chain reaction (RT-PCR) combined with semi-nested PCR. The overall recoveries by elution/concentration were 29.0% from cabbage and 13.7% from lettuce. The whole procedure usually takes 18 hr. The overall detection sensitivity was 100 RT-PCR units of genogroup II norovirus (GII NoV)/25 g cabbage and 100 RT-PCR units of GII NoV/10 g lettuce. The virus detecting method developed in this study should facilitate the detection of low levels of NoV in cabbage and lettuce.

• 대한화학회지
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• 제8권3호
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• pp.103-108
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• 1964

Ac$^{227}$을 溶媒抽出法에 依하여 그의 崩壞系列元素로부터 分離하는 迅束한 方法을 考察해 보았다. HDEHP 및 TBPO로 二段階抽出過程을 거침으로서 追跡子用으로 充分한 純度를 가진 Ac$^{227}$을 얻을 수 있으며 이들 過程中에 일어날 Ac$^{227}$의 損失程度도 推定해 보았다. Ac$^{227}$으로부터 Th$^{227}$의 milking도 兼하여 實驗하였다.

• 대한환경공학회지
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• 제31권1호
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• pp.64-69
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• 2009

수돗물에서 geosmin과 2-MIB에 의한 곰팡이와 흙냄새는 소비자들에 대한 수돗물불신의 주된 요인이다. 따라서 geosmin과 2-MIB의 조기 검출 및 분석방법은 이취미 문제를 예방하는데 있어서 중요한 부분이다. 본 연구에서는 교반막대 추출법 (SBSE)과 GC/MS를 이용하여 geosmin과 2-MIB의 분석방법에 대하여 살펴보았다. 용매를 사용하지 않고 시료를 추출하는 SBSE 방법을 최적화하여 수중의 geosmin과 2-MIB를 분석하는데 적용하였다. 실험 결과 SBSE 방법은 간단하고 신속한 분석이 가능하여 소량(10~20 mL)의 시료를 사용하여 한 번에 많은 시료를 동시에 분석할 수 있었다. 그리고 SBSE 방법은 높은 회수율과 재현성을 보였다. 검출한계는 1~2 ng/L 이었으며 정량한계는 3~6 ng/L 이었다. 무엇보다도 SBSE는 수중의 geosmin과 2-MIB를 분석하는 데에 매우 신뢰성이 있고 실용적인 방법이라고 판단된다.

• Mycobiology
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• 제38권1호
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• pp.33-38
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• 2010

We have successfully applied the nested PCR to detect Cylindrocarpon destructans, a major pathogen causing root rot disease from ginseng seedlings in our former study. The PCR assay, in this study, was used to detect the pathogen from soils. The nested PCR using internal transcribed spacer (ITS) 1, 4 primer set and Dest 1, 4 primer set maintained the specificity in soils containing various microorganisms. For a soil DNA extraction method targeting chlamydospores, when several cell wall disrupting methods were tested, the combination of lyophilization and grinding with glass beads, which broke almost all the chlamydospores, was the strongest. The DNA extraction method which was completed based on the above was simple and time-saving because of exclusion of unnecessary stages, and efficient to apply in soils. As three ginseng fields whose histories were known were analyzed, the PCR assay resulted as our expectation derived from the field information. The direct PCR method will be utilized as a reliable and rapid tool for detecting and monitoring C. destructans in ginseng fields.

• 약학회지
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• 제39권3호
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• pp.283-288
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• 1995

Rapid gas chromatographic profiling method was applied to saliva from healthy subjects for the analysis of free organic acids. Saliva samples were first saturated with NaHCO$_{3}$ and extracted with diethyl ether. The aqueous phase was solid-phase extracted using Chromosorb P as the adsorbent and diethyl ether as the eluent after the acidification and NaCl saturation, followed by triethylamine treatment. The resulting tiiethylammonium salts of acids were directly converted into stable tert.-butyl-dimethylsflyl derivatives, with subsequent analysis by dual-capiuary column gas chromatography and gas chromatograpy -mass spectrometry. From the ten saliva samples studied, twenty eight free organic acids including various fatty acids, hydroxy acids, dicarboxylic acids, md aromatic acids were tentatively identified. Among the acids identified , the concentration of lactic acid was highest for five saliva samples while $\alpha$-hydroxyisocaproic acid was most abundant for me sample, and succinic acid and glycolic acid for two samples. respectively. When the GC profiles were simplified to the corresponding acid retention index spectra of bar graphical form, they presented characteristic patterns for each individual.