• Journal of Microbiology and Biotechnology
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• 제33권5호
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• pp.698-705
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• 2023

Rapid diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) is essential for guiding clinical treatment and preventing the spread of MRSA infections. Herein, we present a simple and rapid MRSA screening test based on the aggregation effect of mannose-binding lectin (MBL)-conjugated gold nanoparticles (AuNP), called the MRSA probe. Recombinant MBL protein is a member of the lectin family and part of the innate immune system. It can recognize wall teichoic acid (WTA) on the membrane of MRSA more specifically than that of methicillin-sensitive Staphylococcus aureus (MSSA) under optimized salt conditions. Thus, the MRSA probe can selectively bind to MRSA, and the aggregation of the probes on the surface of the target bacteria can be detected and analyzed by the naked eye within 5 min. To demonstrate the suitability of the method for real-world application, we tested 40 clinical S. aureus isolates (including 20 MRSA specimens) and recorded a sensitivity of 100%. In conclusion, the MRSA probe-based screening test with its excellent sensitivity has the potential for successful application in the microbiology laboratory.

• 대한세포병리학회지
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• 제1권1호
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• pp.36-42
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• 1990

The fluoroscopy-guided fine needle aspiration biopsy has been gaining widespread acceptance as a rapid and effective method to make a pre-operative diagnosis of mediastinal tumors including thymoma, malignant lymphoma, and metastatic carcinoma. Although thymoma is a most common tumor of the superior mediastinum, most cytopathologists are not experted in cytologic diagnosis of this tumor because of limited experience. In order to define the diagnostic cytologic features of thymoma, we have retrospectively reviewed imprinting smears and corresponding tissue sections from four cases of this tumor. All cases revealed an apparent biphasic pattern of epithelial cell clusters and lymphocytes with occasional branching capillary fronds extending from three dimensional epithelial cell clusters. Epithelial cell clusters predominated in one case and lymphocytes in two cases. Mixed epithelial cell and lymphocyte type represented in one of four cases. In the lymphocyte predominant type, the presence of epithelial cell clusters and small mature lymphocytes are helpful features to differentiate from a malignant lymphoma.

• Tuberculosis and Respiratory Diseases
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• 제74권2호
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• pp.51-55
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• 2013

Acute eosinophilic pneumonia is a severe and rapidly progressive lung disease that can cause fatal respiratory failure. Since this disease exhibits totally different clinical features to other eosinophilic lung diseases (ELD), it is not difficult to distinguish it among other ELDs. However, this can be similar to other diseases causing acute respiratory distress syndrome or severe community-acquired pneumonia, so the diagnosis can be delayed. The cause of this disease in the majority of patients is unknown, even though some cases may be caused by smoke, other patients inhaled dust or drugs. The diagnosis is established by bronchoalveolar lavage. Treatment with corticosteroids shows a rapid and dramatic positive response without recurrence.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2008년도 정기 학술대회
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• pp.127-132
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• 2008

As economic rapid growth, large structures are damaged by defects from design and construction process of the formation and environments. Therefore, safety diagnosis and monitoring skills are bringing importance into relief and legislate for safety diagnosis of large structures. However, the existing monitoring systems are difficult by using wire cable because of cost, error of date, location, revise. In this paper, wireless sensor network which are accelerator, temperature sensor system using ubiquitous had been field test on Sea-Hea Grand bridge. We compare accelerator, temperature sensor system with wire and wireless sensor network.

• The Plant Pathology Journal
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• 제27권4호
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• pp.385-389
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• 2011

A new reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the Potato leafroll virus (PLRV) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to address its advantages over RTPCR. RT-LAMP primers were designed from the open reading frame 3 (ORF3) sequence of PLRV. The RT-LAMP reactions were conducted without or with a set of loop primers. By real-time monitoring using Turbimeter, the RT-LAMP (with loop primers) detects PLRV in less than 30 min, compared to 120 min of RT-PCR. By adding fluorescent reagent during the reaction, final products of the RT-LAMP were fluorescently visualized under UV light or could be differentiated by naked-eye inspection under normal light. The RT-LAMP was extremely sensitive, about 2000-fold more sensitive than RT-PCR. This study presents great potential of the RT-LAMP for diagnosis and PLRV epidemiology because RT-LAMP method is speedy, sensitive, inexpensive, and convenient.

• Journal of applied mathematics & informatics
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• 제8권2호
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• pp.505-518
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• 2001

The huge and complicated plants such as nuclear power stations are likely to cause the operators to make mistakes due to a variety of inexplicable reasons and symptoms in case of emergency. That’s why the prevention system assisting the operators is being developed for. First of all. I suggest an improved fuzzy diagnosis. Secondly, I want to demonstrate that a classification system of nuclear plant’s accident investigating the causes of accidents foresees possible problems, and maintains the reliability of the diagnostic reports in spite of improper working in part. In the event of emergency in a nuclear plant, a lot of operational steps enable the operators to find out what caused the problems based on an emergent operating plan. Our system is able to classify their types within twenty to thirty seconds. As so, we expect the system to put down the accidents right after the rapid detection of the damage control-method concerned.

• Journal of Power Electronics
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• 제18권5호
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• pp.1608-1617
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• 2018

The rapid and correct isolation of faulty submodules (SMs) is of great importance for improving the reliability of modular multilevel converters (MMCs). Therefore, a fast diagnosis method containing fault detection and fault location determination was presented in this paper. An improved incremental predictive model of arm current was proposed to detect failures, and the multi-step prediction method was used to eliminate the negative impact of disturbances. Moreover, a control method was proposed to strengthen the fault characteristics to rapidly locate faulty arms and faulty SMs by detecting the variation rate of the SM capacitor voltage. The proposed method can rapidly and easily locate faulty SMs under different load conditions without the need for additional sensors. The experimental results have validated the effectiveness of the proposed method by using a single-phase MMC with four SMs per arm.

• Journal of Chest Surgery
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• 제25권3호
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• pp.335-340
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• 1992

Truamatic aortic rupture is usually fatal if left untreated. Prompt diagnosis is the cornerstone of suscessful management. But the usual screening tests are non specific and the accuracy of computerized tomography is low, Aortography which is a time consuming procedure may result in false-positive interpretations and significant morbidity. Recently transesophageal echocardiography provides a rapid, effective and relatively safe means of evaluating the distal aortic arch and also affords the opportunity to evaluate the heart during the same study, which may be of benefit in cases of suspected cardiac injury. The experience with the traumatic aortic ruptured patient and a critical review of the literature suggests that transesophageal echocardiography is a useful technique for the diagnosis of ruptured aorta following blunt chest trauma.

• Journal of Korean Neurosurgical Society
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• 제53권3호
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• pp.183-186
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• 2013

Septic internal jugular vein-sigmoid sinus thrombosis (IJV-SST) associated with a malpositioned central venous catheter is a rare condition. It is potentially life-threatening and necessitates early diagnosis and rapid administration of appropriate medications. Unfortunately, it is difficult to diagnose due to vague clinical presentations. Several studies such as CT, MRI, and cerebral angiography should be performed and carefully examined to help make the diagnosis. We report a case of septic IJV-SST due to a malpositioned central venous catheter.

• Journal of Chest Surgery
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• 제23권5호
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• pp.987-993
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• 1990

An unusual but often lethal complication of mitral valve replacement is rupture of the left ventricle. From March 1977 through June 1990, 424 mitral valve replacements were performed as isolated or combined procedures. Rupture of the posterior wall of the left ventricle was observed in 3 patients. Their was one type I and two type II rupture. Once the diagnosis was made, all of the patient were connected to the heart-lung machine again and total cardiopulmonary bypass is re-established. Repair was attempted in all of them from the outside of the heart. One of them was successively repaired but two were failed due to myocardial ischemia by circumflex coronary artery injury and failure of adequate closure of the ruptured site. From this results, we concluded that prevention is the best solution. But if we encountered this condition, early diagnosis and rapid treatment may improve the patient`s chances for survival.